##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53624
Sequences flagged as poor quality	0
Sequence length	56
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.42016634342831	39.0	38.0	39.0	33.0	40.0
2	37.2031553035954	39.0	38.0	39.0	33.0	40.0
3	35.50814933611816	38.0	36.0	39.0	25.0	40.0
4	34.63120990601223	39.0	35.0	39.0	17.0	40.0
5	36.71488512606296	39.0	36.0	39.0	30.0	40.0
6	36.97282933015068	39.0	38.0	40.0	31.0	40.0
7	37.202819633000146	39.0	38.0	40.0	33.0	40.0
8	36.89972773385051	39.0	37.0	39.0	31.0	40.0
9	36.83100850365508	39.0	37.0	39.0	31.0	40.0
10	36.2962852454125	38.0	36.0	39.0	29.0	40.0
11	35.506340444577056	38.0	35.0	39.0	29.0	40.0
12	33.32979635983888	38.0	31.0	39.0	19.0	40.0
13	32.74701626137551	37.0	30.0	39.0	17.0	40.0
14	32.328509622557064	37.0	29.0	39.0	16.0	40.0
15	32.90120095479636	37.0	30.0	39.0	18.0	40.0
16	32.456605251379976	36.0	30.0	39.0	18.0	40.0
17	32.50861554527823	36.0	30.0	39.0	18.0	40.0
18	32.5372594360734	36.0	30.0	38.0	19.0	40.0
19	33.53220572877816	36.0	31.0	38.0	24.0	40.0
20	33.83255631806654	36.0	31.0	39.0	24.0	40.0
21	35.32711099507683	38.0	35.0	39.0	27.0	40.0
22	34.94616216619424	38.0	34.0	39.0	26.0	40.0
23	35.182959122780844	38.0	35.0	39.0	27.0	40.0
24	34.793954199612116	38.0	34.0	39.0	26.0	40.0
25	35.10646352379531	38.0	35.0	39.0	27.0	40.0
26	35.5126249440549	38.0	35.0	39.0	29.0	40.0
27	35.504233179173504	38.0	35.0	39.0	29.0	40.0
28	35.47924436819335	38.0	35.0	39.0	29.0	40.0
29	35.310085036550795	38.0	35.0	39.0	28.0	40.0
30	35.166940176040576	38.0	35.0	39.0	27.0	40.0
31	34.9765403550649	38.0	35.0	39.0	27.0	40.0
32	33.9254624794868	38.0	33.0	39.0	20.0	40.0
33	35.10737729374907	38.0	35.0	39.0	28.0	40.0
34	34.88503282112487	38.0	35.0	39.0	27.0	40.0
35	34.8852006564225	38.0	35.0	39.0	27.0	40.0
36	32.577614500969716	35.0	31.0	38.0	23.0	39.0
37	32.38378338057586	35.0	31.0	38.0	22.0	39.0
38	31.57757720423691	35.0	31.0	38.0	15.0	39.0
39	29.60379680739967	34.0	27.0	38.0	5.0	39.0
40	25.251771594808293	31.0	11.0	38.0	4.0	39.0
41	21.824500223780397	26.0	9.0	33.0	4.0	37.0
42	25.810122333283605	30.0	21.0	34.0	4.0	37.0
43	21.82343726689542	26.0	8.0	33.0	4.0	37.0
44	22.860305087274355	28.0	10.0	33.0	4.0	37.0
45	25.515422199015365	30.0	21.0	34.0	4.0	38.0
46	25.55439728479785	30.0	16.0	36.0	4.0	38.0
47	24.745710875727287	30.0	10.0	36.0	4.0	38.0
48	24.731817842756975	30.0	11.0	35.0	4.0	38.0
49	22.02290019394301	26.0	5.0	35.0	4.0	38.0
50	24.513296285245413	30.0	8.0	35.0	4.0	38.0
51	26.113904221990154	32.0	4.0	38.0	4.0	40.0
52	25.88436147993436	32.0	4.0	38.0	4.0	40.0
53	25.513650604207072	32.0	4.0	38.0	4.0	40.0
54	24.622594360734	31.0	4.0	38.0	4.0	40.0
55	24.86334477099806	32.0	4.0	38.0	4.0	40.0
56	23.89392809189915	31.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.6117906799238426
1	2	1.850892648063983
1	3	3.852878471650037
1	4	5.304177364182408
1	5	1.9933304590873746
1	6	1.99699673599995
1	7	2.078236223777836
1	8	2.2936921026531607
1	9	2.4876057765476034
1	10	2.2210002702751197
1	11	-2.7744756626757834
1	12	-6.0503248698387
1	13	-20.822017822563247
1	14	-17.32951570050767
1	15	-10.879723794460212
1	16	-20.6245761725402
1	17	-21.47391101668688
1	18	-20.31586671684603
1	19	-5.906117444168707
1	20	-10.775110627972992
1	21	-6.897122014987538
1	22	-2.5133841549798923
1	23	-3.06317290623074
1	24	-3.2214747120483587
1	25	-3.5490088558709765
1	26	2.7290174046448925
1	27	3.0909118123753387
1	28	2.904556934957199
1	29	3.3339359963301476
1	30	3.140622431042516
1	31	1.5284507371006129
1	32	-10.844790101165103
1	33	1.3832925409982337
1	34	1.0561384994964484
1	35	1.3959457512004079
1	36	4.333525907812774
1	37	5.034605984221056
1	38	5.662257682172768
1	39	7.783925090498094
1	40	10.560741813217579
1	41	-1.7048305721050987
1	42	-6.973325159862217
1	43	-12.242699473675929
1	44	-15.724987306715693
1	45	-12.556448484211863
1	46	-18.67725296300398
1	47	-17.898939054570203
1	48	-16.653763653127786
1	49	-15.241894353492668
1	50	-16.414589612436068
1	51	-17.8976731336274
1	52	-17.743929911607836
1	53	-17.611441189273425
1	54	-18.004169322971844
1	55	-16.83059853285245
1	56	-15.51074900775391
2	1	0.7840372742438646
2	2	1.3844876607631278
2	3	3.0198145141344526
2	4	4.797827791335493
2	5	1.4486525556008871
2	6	1.5068097122179154
2	7	1.3139785465670286
2	8	1.7755669583446831
2	9	1.798388505709461
2	10	2.0725357124362844
2	11	-3.0637852909371546
2	12	-10.3844278483656
2	13	-13.257483073082753
2	14	-15.883891133433094
2	15	-6.885727026970024
2	16	-4.753414777943107
2	17	-3.4376607280699325
2	18	-11.039487127836566
2	19	-2.6731458440763483
2	20	-4.277996797448864
2	21	2.02622132373331
2	22	2.400261654302028
2	23	1.7516504601973395
2	24	2.145184258166374
2	25	1.7407625595065355
2	26	2.1120698174825776
2	27	1.7990623549752058
2	28	2.2107217929576635
2	29	1.9033195105301033
2	30	1.7185490068910525
2	31	-1.5853797870277688
2	32	-11.150262278491358
2	33	-2.1907088443631153
2	34	-1.237788999926316
2	35	-2.035594308831918
2	36	4.665781737875118
2	37	4.673026672283864
2	38	5.654176407640332
2	39	7.8478826060834095
2	40	10.311376770502275
2	41	7.876097619998344
2	42	3.183451566067127
2	43	-1.697097995957158
2	44	-9.440757336731856
2	45	1.0604991029504554
2	46	-11.636384803225639
2	47	-11.435304789966185
2	48	-8.77913885515965
2	49	-12.184863644649445
2	50	-8.537424985329164
2	51	-8.154195876642872
2	52	-8.05725246990846
2	53	-7.610748508599215
2	54	-14.957067037125618
2	55	-7.5101182742516635
2	56	-6.910194863214542
3	1	1.4545217193761957
3	2	1.9569996558103355
3	3	3.9579045930695997
3	4	5.426677814551709
3	5	2.278864597080535
3	6	2.3551304056090316
3	7	2.1245341881085906
3	8	2.410607973667389
3	9	2.4568005162341464
3	10	2.357011799729271
3	11	1.2956018008443024
3	12	2.068752773829104
3	13	-5.170963958394626
3	14	-4.506240614937504
3	15	-3.003665426598566
3	16	2.513416981846401
3	17	2.874674823702236
3	18	2.473900892593708
3	19	1.2875413560474698
3	20	1.168683283034035
3	21	3.25312118443599
3	22	3.9438307612514194
3	23	3.2946004679360783
3	24	3.2960896907619457
3	25	2.9644121493533078
3	26	2.230008217111113
3	27	2.341857587911278
3	28	2.4343713828044358
3	29	2.9853501559410347
3	30	2.493351669017656
3	31	2.855418850953967
3	32	1.231412384873444
3	33	1.7320585291084853
3	34	2.3571653415068994
3	35	2.693549786509358
3	36	5.473860982574077
3	37	5.6278324299065225
3	38	5.808177336289145
3	39	8.03252894565933
3	40	11.922118141807022
3	41	11.369210141279876
3	42	8.668361022807574
3	43	4.122279809336142
3	44	5.237001524125596
3	45	9.184318945080157
3	46	3.6381856966969757
3	47	-2.681861050606951
3	48	2.3384899948635685
3	49	-8.712800496529958
3	50	2.231960558704266
3	51	0.666729676677047
3	52	1.2479634409412554
3	53	-0.2032264531898349
3	54	-4.597215620936176
3	55	-0.5200238616220396
3	56	2.7191194844096636
4	1	1.8760458474220627
4	2	2.267678107793138
4	3	3.8142113298111227
4	4	5.672138875230161
4	5	2.080465170420233
4	6	2.447103649756187
4	7	2.4998328025376395
4	8	2.547612273715167
4	9	2.583293588379391
4	10	2.521249735706334
4	11	2.6127303244945637
4	12	3.1688483304464015
4	13	5.186178898748231
4	14	2.5952882909392265
4	15	5.503740211241858
4	16	6.097626250838278
4	17	5.86607472814562
4	18	5.837732712947268
4	19	4.717546133878336
4	20	4.573962786139624
4	21	3.758615689930494
4	22	3.621327177878271
4	23	3.21337734323469
4	24	3.6035431069110118
4	25	3.0409768889615236
4	26	2.7769504053576526
4	27	2.2339980561387094
4	28	2.043544818064831
4	29	2.461341555845472
4	30	2.5960750326105853
4	31	1.9767757549195721
4	32	3.3407052659054557
4	33	2.212230531019614
4	34	2.551861949246984
4	35	2.30809828149264
4	36	4.86862925777714
4	37	4.360871130336523
4	38	5.49618498081853
4	39	8.059762581588615
4	40	11.007641314286719
4	41	5.624107417916285
4	42	9.261528724670924
4	43	8.809331887692569
4	44	12.008262871473903
4	45	9.656488080292768
4	46	8.967258797509203
4	47	8.886342484987889
4	48	9.114768064619899
4	49	4.799980201033346
4	50	9.261105326979472
4	51	6.837537130093196
4	52	6.764088620109913
4	53	6.397824669600418
4	54	2.7346047326233105
4	55	6.460028694517472
4	56	5.8814462880408165
5	1	2.222241499595974
5	2	2.814417238227918
5	3	4.463124373289382
5	4	6.046595396969295
5	5	2.8261173443332765
5	6	3.09166886714749
5	7	2.8395067155811162
5	8	2.9900035780629963
5	9	3.24416315359678
5	10	2.7919019096193836
5	11	3.958925976668475
5	12	6.702543982620313
5	13	6.8611788987482285
5	14	5.896918725721836
5	15	7.0401532547201136
5	16	7.598169729099144
5	17	6.920966032493446
5	18	7.033928365121181
5	19	5.37298091648703
5	20	5.566897568748317
5	21	4.008615689930494
5	22	4.686001090921749
5	23	3.4220729954086053
5	24	5.01930397647623
5	25	4.120868193309349
5	26	2.673689535792434
5	27	2.9562806648343596
5	28	3.3033274267604824
5	29	3.3765589471498245
5	30	3.23140111956711
5	31	3.6702540157891335
5	32	4.186357439818501
5	33	2.1703827049326634
5	34	3.5942532535948146
5	35	3.357011324970898
5	36	4.5175423012554
5	37	4.779892869466959
5	38	5.979880632992444
5	39	7.488023451153836
5	40	5.352750009938891
5	41	2.2697595918293274
5	42	8.514789594236142
5	43	12.356071018127349
5	44	11.555545480169549
5	45	9.561379384640595
5	46	10.91291097142225
5	47	11.430364224118321
5	48	11.756072412445985
5	49	12.275523679294217
5	50	11.550779240022951
5	51	11.006015390962762
5	52	10.481479924457737
5	53	9.893476843513458
5	54	10.305256906536354
5	55	9.707311303213125
5	56	8.365685418475596
6	1	1.8895034043578818
6	2	2.5870362858469704
6	3	3.9881243732893807
6	4	5.735285873159768
6	5	2.6451649633809
6	6	2.4000022004808272
6	7	2.5567686203430213
6	8	2.7530988161582357
6	9	2.617972677406307
6	10	2.6454733381908113
6	11	3.7690450242875286
6	12	6.551353506429841
6	13	5.805226517795852
6	14	6.858228249531361
6	15	6.546700873767737
6	16	6.772574491003905
6	17	6.857275556302973
6	18	6.517856936549752
6	19	5.550361868867981
6	20	5.740111854462604
6	21	3.865758547073348
6	22	4.737786805207463
6	23	3.822072995408604
6	24	3.96275635742861
6	25	3.9488443837855414
6	26	2.2629752500781493
6	27	2.9306854267391174
6	28	2.65689885533191
6	29	2.621797042387918
6	30	3.3843773100432983
6	31	4.341682587217704
6	32	5.179214582675648
6	33	3.4078827049326605
6	34	4.435919920261476
6	35	3.8986779916375625
6	36	4.5425423012553985
6	37	4.305488107562201
6	38	4.759642537754349
6	39	7.014213927344308
6	40	3.4331071527960333
6	41	0.5340453061150434
6	42	7.323122927569475
6	43	12.319166256222587
6	44	10.693045480169552
6	45	8.266736527497738
6	46	11.055768114279395
6	47	10.866673747927848
6	48	11.434643841017415
6	49	12.236833203103743
6	50	10.675184001927708
6	51	10.190539200486572
6	52	10.874337067314883
6	53	10.91490541494203
6	54	11.096923573203018
6	55	10.10909701749884
6	56	11.081756847047028
7	1	1.5814081662626407
7	2	2.1260839048945854
7	3	3.258124373289384
7	4	5.462428730302626
7	5	1.7461173443332783
7	6	2.6166688671474887
7	7	2.638673382247781
7	8	2.9616702447296603
7	9	3.107496486930117
7	10	2.786901909619381
7	11	4.004759310001809
7	12	5.864210649286981
7	13	6.646178898748232
7	14	7.261085392388505
7	15	6.997653254720117
7	16	7.194003062432476
7	17	6.883466032493445
7	18	6.519761698454513
7	19	5.381314249820363
7	20	5.907730902081653
7	21	3.3586156899304953
7	22	4.1535010909217505
7	23	3.622072995408601
7	24	4.461803976476233
7	25	3.47503485997602
7	26	1.9220228691257688
7	27	2.965447331501025
7	28	2.424994093427145
7	29	2.2432256138164917
7	30	2.7305677862337774
7	31	2.0735873491224694
7	32	3.2863574398185023
7	33	1.3478827049326583
7	34	2.6692532535948104
7	35	1.538677991637563
7	36	0.4725423012553982
7	37	0.8673928694669613
7	38	1.6515472996591107
7	39	3.391356784487165
7	40	-3.7297499900611086
7	41	-10.233573741504005
7	42	4.46645626090281
7	43	8.65107101812735
7	44	6.806378813502885
7	45	3.9038793846405966
7	46	8.07291097142225
7	47	8.539530890784988
7	48	8.849405745779318
7	49	10.636357012627549
7	50	8.046612573356281
7	51	9.661015390962763
7	52	10.22481325779107
7	53	10.120143510180124
7	54	9.850256906536355
7	55	10.324811303213124
7	56	9.94651875180893
8	1	2.2597414995959753
8	2	2.5269172382279166
8	3	3.975624373289378
8	4	5.896595396969296
8	5	2.1386173443332765
8	6	2.479168867147493
8	7	2.602006715581119
8	8	2.9150035780629935
8	9	2.9191631535967844
8	10	2.2794019096193807
8	11	3.633925976668479
8	12	5.977543982620318
8	13	6.6736788987482285
8	14	6.959418725721836
8	15	6.365153254720116
8	16	6.585669729099148
8	17	6.183466032493449
8	18	5.858928365121184
8	19	4.860480916487035
8	20	4.26689756874832
8	21	3.258615689930494
8	22	2.948501090921745
8	23	3.197072995408604
8	24	3.9693039764762332
8	25	3.233368193309353
8	26	2.7736895357924354
8	27	2.8312806648343596
8	28	2.4658274267604767
8	29	3.026558947149823
8	30	3.1939011195671085
8	31	3.707754015789135
8	32	4.198857439818504
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115	22	-0.7931655757449221
115	23	-0.1334825601469518
115	24	0.5373595320317861
115	25	0.14170152664268443
115	26	0.9931339802368768
115	27	0.041002887056578174
115	28	-0.43278368435063186
115	29	0.9765589471498259
115	30	1.4327900084559957
115	31	0.7091429046780249
115	32	-6.446975893514832
115	33	-0.3587839617340052
115	34	0.1581421424836975
115	35	0.743122436082011
115	36	3.3036534123665113
115	37	3.111837313911405
115	38	4.349325077436891
115	39	7.004690117820502
115	40	8.901361121050005
115	41	8.953092925162661
115	42	2.3286784831250316
115	43	1.0866265736829064
115	44	-1.073621186497114
115	45	1.4905460513072626
115	46	-2.9093112507999734
115	47	-6.073802442548345
115	48	-6.221705365331793
115	49	-6.71475409848356
115	50	-0.7089429821992752
115	51	-0.0634290534816806
115	52	0.7537021466799594
115	53	-0.4176342675976521
115	54	0.3969235732030185
115	55	1.0670335254353454
115	56	4.075407640697819
116	1	0.7109319757864512
116	2	1.2060839048945837
116	3	2.1666958018608113
116	4	2.842428730302629
116	5	0.4308792490951845
116	6	1.435716486195112
116	7	1.390101953676357
116	8	1.5388131018725204
116	9	1.0227345821682121
116	10	2.0145209572384317
116	11	-1.4809549757124714
116	12	-4.079598874522542
116	13	-11.599535386966053
116	14	-13.022724131421022
116	15	-6.798537221470358
116	16	-13.072663604234187
116	17	-10.34510539607798
116	18	-6.244047825355008
116	19	-4.675828607322494
116	20	-5.640840526489775
116	21	1.115758547073348
116	22	1.7973106147312734
116	23	1.0601682335038447
116	24	0.9151373098095661
116	25	1.2226539075950669
116	26	1.048689535792434
116	27	1.3235425695962633
116	28	0.6926131410461949
116	29	1.0384637090545823
116	30	1.6581868338528238
116	31	-0.7892697937346753
116	32	-6.4041187506576875
116	33	0.07454937159932484
116	34	-0.7783657940242392
116	35	-0.19656010360053244
116	36	3.268732777445873
116	37	3.13882144089553
116	38	4.652499680611495
116	39	6.228499641630023
116	40	9.814059533748416
116	41	3.986426258495996
116	42	1.239789594236143
116	43	-11.240357553301221
116	44	-1.8307640436399737
116	45	0.052450813212022496
116	46	-5.539469980958703
116	47	-8.573802442548343
116	48	-8.886784730411158
116	49	-8.132214415943878
116	50	-4.634339807596099
116	51	-0.33327032332295303
116	52	-0.4351867422089306
116	53	-2.0255707755341597
116	54	-4.307838331558884
116	55	-2.1647125063106856
116	56	0.0817568470470249
117	1	-0.38594815557643614
117	2	0.40148620374515787
117	3	1.4967450629445551
117	4	1.35392298317619
117	5	0.267496654678105
117	6	0.09253093611300756
117	7	0.13476533627077458
117	8	0.5667277159940269
117	9	0.1286459122174719
117	10	0.12207432341248392
117	11	-9.356160230228074
117	12	-12.343145672552097
117	13	-8.555200411596598
117	14	-8.579374377726442
117	15	-7.425795021141951
117	16	-8.29105440883189
117	17	-5.6406718985410365
117	18	-3.6052957728098534
117	19	-3.3244328766164166
117	20	-3.0628438105620255
117	21	-0.7931084480005381
117	22	1.1355700564389863
117	23	0.9944867885120487
117	24	-1.8319891269720472
117	25	0.4911268139990099
117	26	-0.45131046420756604
117	27	-1.2019089903380547
117	28	0.51281018538117
117	29	-0.8786134666432801
117	30	-0.5043747425018594
117	31	-3.0142287428315555
117	32	-14.920539111905637
117	33	-2.643841432998375
117	34	-2.351436401577601
117	35	-2.7335059164084115
117	36	-2.4833197677101175
117	37	-1.9326071305330395
117	38	-1.4813262635592785
117	39	1.606988968395214
117	40	1.3764569064906134
117	41	-0.6629990288603267
117	42	-3.8636586816259246
117	43	-10.358584154286444
117	44	-6.634540726727
117	45	-4.548534408462853
117	46	-7.500882132026026
117	47	-10.096790948295471
117	48	-8.209444828933325
117	49	-6.709390113809231
117	50	-7.2591345530805
117	51	-5.343122540071722
117	52	-4.889209730714676
117	53	-5.320316259934817
117	54	-10.000777576222267
117	55	-6.458636972648945
117	56	-4.001986995317505
118	1	0.2617685266230012
118	2	0.6204983193090001
118	3	0.41380004896505795
118	4	0.7928791807530757
118	5	0.4315227497386829
118	6	0.8977499482285722
118	7	0.24660131017571274
118	8	1.2041927672521808
118	9	0.6617307211643464
118	10	1.6020370447545176
118	11	-7.366411861169361
118	12	-9.479212774136442
118	13	-11.28679407422474
118	14	-3.300716409413301
118	15	-18.781806204739343
118	16	-7.523114054684637
118	17	-8.700317751290335
118	18	-4.864382445689628
118	19	-3.989856921350807
118	20	-3.896953782603031
118	21	0.191048122362929
118	22	0.9762037936244496
118	23	-0.47522430188869436
118	24	-1.7425203478480924
118	25	0.8191790041201656
118	26	-0.23509424799134848
118	27	-0.3109490648953681
118	28	-0.5757266272935766
118	29	-0.06127889068801551
118	30	0.29525247091846296
118	31	-1.9797459842108651
118	32	-9.14607499261393
118	33	-1.1461713491213956
118	34	-0.9199359355943741
118	35	-0.7139346209750457
118	36	2.143893652606753
118	37	2.2565820586561465
118	38	2.9427184708302825
118	39	5.754239667370051
118	40	7.411871631560512
118	41	6.630570402640139
118	42	0.6722220266685746
118	43	1.4932331802895114
118	44	-1.7529004657763956
118	45	1.434690195451406
118	46	-4.573575515064238
118	47	-8.055784424530327
118	48	-6.892576236202665
118	49	-5.726165509894972
118	50	-3.4650991383554306
118	51	-5.3216873117399395
118	52	-5.322574129596315
118	53	-6.852469102432487
118	54	-12.069743093463646
118	55	-9.625458967057146
118	56	-6.076544311254132
119	1	0.34585261070709095
119	2	0.567195016005698
119	3	2.2381243732893807
119	4	2.453539841413736
119	5	1.0261173443332794
119	6	0.6500022004808272
119	7	0.45756227113667336
119	8	0.6538924669518806
119	9	0.816385375819003
119	10	-0.171986979269505
119	11	-7.94524068999819
119	12	-11.055789350713017
119	13	-3.202709990140658
119	14	-2.7489146076114963
119	15	-6.155680078613216
119	16	-11.850441382011965
119	17	-10.08320063417322
119	18	-2.859127190434375
119	19	-2.068685750179636
119	20	-3.6289357645850124
119	21	-1.1302731989583918
119	22	-1.0320544646338092
119	23	-1.5668158934802818
119	24	-5.77930713463488
119	25	-1.2416318066906484
119	26	0.8542450913479911
119	27	1.2243362203899153
119	28	0.8116607600938153
119	29	0.6098922804831588
119	30	1.0272344529004442
119	31	-2.146412650877533
119	32	-11.519198115737055
119	33	-0.25878396173400375
119	34	-1.3974134130718525
119	35	-0.8513220083624375
119	36	3.4869867456998414
119	37	3.7618373139114034
119	38	4.271547299659108
119	39	6.204690117820498
119	40	10.179138898827777
119	41	6.15309292516266
119	42	0.7120118164583644
119	43	-1.0578178707615393
119	44	-1.8902878531637803
119	45	-1.4038983931371831
119	46	-6.448200139688861
119	47	-7.934913553659456
119	48	-10.32726092088735
119	49	-7.092531876261338
119	50	-7.308942982199273
119	51	-10.007873497926127
119	52	-10.546297853320041
119	53	-11.01763426759765
119	54	-9.986409760130313
119	55	-12.29963314123132
119	56	-8.87459235930218
120	1	0.7347414995959767
120	2	1.0100054735220354
120	3	2.1498890791717358
120	4	2.8326248087339962
120	5	1.1437644031568084
120	6	1.0225512200886655
120	7	0.9755361273458192
120	8	0.3973565192394659
120	9	1.3000455065379555
120	10	0.6727842625605547
120	11	-9.587397552743287
120	12	-2.281279546791449
120	13	-7.313821101251769
120	14	-5.371463627219342
120	15	-5.587052627632826
120	16	-9.268742035606738
120	17	-6.9459457322124365
120	18	-2.4653363407611693
120	19	-2.769666142336497
120	20	-3.0456024312516803
120	21	-0.5943254865400931
120	22	0.3029128556276319
120	23	-0.6485152398855121
120	24	-6.153490141170828
120	25	-1.2857494537494745
120	26	1.283983653439492
120	27	0.41878066483435816
120	28	0.3606803679369506
120	29	1.4236177706792361
120	30	1.5027246489788766
120	31	-0.9209224547990971
120	32	-8.095995501357969
120	33	-1.2097643538908684
120	34	-8.701334981699304
120	35	-2.5915180867938084
120	36	-0.883928286979895
120	37	-0.5796659540624525
120	38	-0.1304134846546141
120	39	2.1556705099773623
120	40	-0.32739704888463805
120	41	1.5256419447705056
120	42	-2.6719751116462085
120	43	0.8016592534214659
120	44	-2.954013343359861
120	45	-10.964355909477051
120	46	-13.08708902857775
120	47	-14.348312246469913
120	48	-11.390986411083427
120	49	-15.095799850117547
120	50	-11.645544289388816
120	51	-11.677808138449002
120	52	-11.621461252012852
120	53	-11.612405509427715
120	54	-11.981507799346
120	55	-11.766953402669229
120	56	-7.493873405053813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	406.0
5	15.0
6	46.0
7	46.0
8	107.0
9	153.0
10	89.0
11	78.0
12	70.0
13	93.0
14	106.0
15	171.0
16	250.0
17	342.0
18	477.0
19	560.0
20	679.0
21	890.0
22	1091.0
23	1297.0
24	1471.0
25	1600.0
26	1673.0
27	1727.0
28	1817.0
29	1965.0
30	2195.0
31	2482.0
32	2914.0
33	3625.0
34	4367.0
35	5196.0
36	5858.0
37	5568.0
38	3252.0
39	948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.40325227510071	0.5053707295240937	0.05594509920930926	0.03543189616589587
8	99.3678203789348	0.48858719976130094	0.07272862897210204	0.07086379233179174
9	96.22092481020687	2.1823879427729382	0.820727089589823	0.7759601574303782
10	43.35931672385499	34.7139340593764	8.740489333134418	13.186259883634193
11	30.51675586968279	28.82158775152453	19.13544561102512	21.526210767767562
12	34.39504699388334	14.469267492167686	22.6838728927346	28.451812621214383
13	28.56183798299269	13.51447113232881	24.98881098015814	32.934879904520365
14	20.982242781466837	17.852346489591884	28.174662389017385	32.9907483399239
15	24.193126634292117	19.129622711991033	26.101979828165856	30.575270825550987
16	27.588008133873103	18.86275021920415	26.306363449806913	27.242878197115832
17	27.04785001399123	24.150732207816436	21.208842458725865	27.592575319466466
18	28.154724159797084	26.47431832593532	21.916147562385767	23.45480995188183
19	27.43653354721979	27.539124433418515	18.207084367014232	26.817257652347465
20	27.254071600470127	25.951905676921065	21.176053579090723	25.61796914351809
21	27.37327017046514	22.958707896601886	24.342571524488047	25.325450408444926
22	26.337087871102494	23.414888855736237	24.51327763687901	25.734745636282263
23	27.806579143667015	25.208861703714753	25.475533343279128	21.509025809339104
24	25.930553483514846	24.955243920632554	21.850290914515888	27.263911681336715
25	25.179493873897396	25.302575387427034	22.880107416593624	26.637823322081942
26	22.936979341775935	26.314019948447832	26.16832903731929	24.580671672456948
27	22.080465558726427	21.03967321358626	28.838154925111446	28.041706302575868
28	20.590542357687134	24.30897953085313	30.007096966980427	25.093381144479306
29	21.051355233458317	23.31971570876938	31.97903258902755	23.649896468744753
30	19.76121630444921	24.70105400615614	35.17022665796101	20.367503031433635
31	20.70782569698782	23.81157037147769	35.071754241722104	20.408849689812392
32	24.75661158566153	19.517699280092508	35.2586817859674	20.467007348278564
33	22.66816143497758	20.859491778774288	38.243647234678626	18.22869955156951
34	20.759682687820813	21.831077928138125	41.7974801679888	15.611759216052262
35	20.528751072801224	24.150154856524498	39.63767304750177	15.683421023172507
36	17.863479316462787	25.460827341488468	38.25567279858063	18.42002054346811
37	18.90002243326105	24.973827862110223	37.94959994017797	18.17654976445076
38	22.472497712033768	25.698063166545264	37.459143460152035	14.370295661268933
39	18.9361741963266	21.31021699649885	33.83010990245455	25.92349890472
40	13.036542106942436	16.615679135151368	27.535143859912537	42.812634897993654
41	13.998908687225997	18.98884226767268	30.092008956291043	36.92024008881028
42	16.41065889299996	26.439810143140114	39.64196285084277	17.507568113017154
43	10.239137756820478	14.385689158339881	31.34987463044048	44.02529845439916
44	12.262647847194886	15.827850568895387	28.560985210594385	43.348516373315334
45	17.589394080851463	23.428251330407992	39.760993371300536	19.221361217440013
46	16.79171332586786	24.089212392683837	41.215005599104146	17.904068682344157
47	16.73413801466336	22.17599761207395	37.02964386321661	24.06022051004608
48	17.09647322261616	25.109162157118863	40.082104870311625	17.71225974995335
49	12.968332055143	16.286638858233104	28.34028544172387	42.40474364490002
50	15.355721253574032	25.48447982657771	42.183850049522526	16.975948870325727
51	18.65748451447499	25.20538203866235	39.26492879465539	16.872204652207273
52	16.0030699537635	26.26682390820089	38.776885494468466	18.953220643567136
53	16.4881986881202	26.69357702154697	40.08334734914317	16.73487694118966
54	14.899258678318676	23.43846283121394	35.89714861912498	25.765129871342406
55	16.579788128468135	24.63053266819872	41.15240177120116	17.63727743213198
56	15.968977761166137	26.24743038684358	38.23397495795178	19.549616894038497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	4.0
7	7.0
8	16.0
9	25.0
10	59.5
11	94.0
12	116.5
13	172.0
14	205.0
15	252.0
16	299.0
17	350.0
18	401.0
19	504.5
20	608.0
21	767.0
22	1187.0
23	1448.0
24	1763.0
25	2078.0
26	2299.5
27	2521.0
28	2615.5
29	2627.5
30	2545.0
31	2230.0
32	1915.0
33	1720.0
34	1525.0
35	1405.0
36	1285.0
37	1227.0
38	1123.0
39	1077.0
40	1075.5
41	1074.0
42	1080.0
43	1086.0
44	1183.5
45	1281.0
46	1332.5
47	1493.5
48	1603.0
49	1694.0
50	1785.0
51	1987.0
52	2189.0
53	2388.0
54	2702.0
55	2817.0
56	2990.5
57	3164.0
58	3089.5
59	3015.0
60	2861.0
61	2707.0
62	2540.5
63	2057.5
64	1741.0
65	1564.5
66	1388.0
67	1175.0
68	962.0
69	779.5
70	597.0
71	499.0
72	340.5
73	280.0
74	202.5
75	125.0
76	90.5
77	56.0
78	39.0
79	14.0
80	6.0
81	3.5
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.024242876324034015
10	0.0
11	0.0018648366403103088
12	0.0
13	0.0
14	0.022378039683723705
15	0.15664627778606594
16	0.039161569446516484
17	0.03543189616589587
18	0.011189019841861853
19	0.024242876324034015
20	0.039161569446516484
21	0.011189019841861853
22	0.0
23	0.0
24	0.0
25	0.0018648366403103088
26	0.16037595106668656
27	0.020513203043413395
28	0.1491869312248247
29	0.03170222288527525
30	0.03543189616589587
31	0.20140235715351337
32	0.011189019841861853
33	0.19394301059227212
34	0.09137699537520513
35	0.04848575264806803
36	0.1473220945845144
37	0.24615843652096076
38	0.15478144114575565
39	0.39721020438609583
40	0.6396389676264359
41	0.8895270774280173
42	0.20513203043413397
43	0.3394002685364762
44	0.5128300760853349
45	0.12867372818141132
46	0.08205281217365358
47	0.039161569446516484
48	0.0634044457705505
49	0.30396837237058033
50	0.2107265403550649
51	0.34872445173802774
52	0.3785618379829927
53	0.2107265403550649
54	0.13240340146203192
55	0.1883485006713412
56	0.2125913769953752
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	53624.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.46598537968075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.06395581694386	83.21460540056691
2	4.005143447374523	7.086379233179175
3	0.9738822487826472	2.584663583470088
4	0.32673538649634265	1.1561987169923915
5	0.19604123189780562	0.8671490377442936
6	0.08221083918295073	0.4363717738326123
7	0.08221083918295073	0.5091004028047144
8	0.04426737494466578	0.31329255557213187
9	0.033727523767364403	0.26853647620468446
>10	0.18128544024958368	2.802849470386394
>50	0.008431880941841101	0.4848575264806803
>100	0.0021079702354602752	0.2759958227659257
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	148	0.2759958227659257	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	72	0.13426823810234223	No Hit
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC	66	0.12307921826048038	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	65	0.12121438162017008	No Hit
TTAACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA	57	0.1062956884976876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0018648366403103088	0.0	0.0	0.0	0.0
10	0.007459346561241235	0.0	0.0	0.0	0.0
11	0.01678352976279278	0.0	0.0	0.0	0.0
12	0.0205132030434134	0.0	0.0	0.0	0.0
13	0.022378039683723705	0.0	0.0	0.0	0.0
14	0.024242876324034015	0.0	0.0	0.0	0.0
15	0.02797254960465463	0.0	0.0	0.0	0.0
16	0.03170222288527525	0.0	0.0	0.0	0.0
17	0.03543189616589587	0.0	0.0	0.0	0.0
18	0.0410264060868268	0.0	0.0	0.0	0.0
19	0.042891242727137104	0.0	0.0	0.0	0.0
20	0.050350589288378336	0.0	0.0	0.0	0.0
21	0.05221542592868865	0.0	0.0	0.0	0.0
22	0.05221542592868865	0.0	0.0	0.0	0.0
23	0.054080262568998956	0.0	0.0	0.0	0.0
24	0.057809935849619576	0.0	0.0	0.0	0.0
25	0.06899895569148143	0.0	0.0	0.0	0.0
26	0.07459346561241235	0.0	0.0	0.0	0.0
27	0.07832313889303297	0.0	0.0	0.0	0.0
28	0.08018797553334328	0.0	0.0	0.0	0.0
29	0.08951215873489482	0.0	0.0	0.0	0.0
30	0.09137699537520513	0.0	0.0	0.0	0.0
31	0.09510666865582575	0.0	0.0	0.0	0.0
32	0.09510666865582575	0.0	0.0	0.0	0.0
33	0.09697150529613606	0.0	0.0	0.0	0.0
34	0.09697150529613606	0.0	0.0	0.0	0.0
35	0.09883634193644637	0.0	0.0	0.0	0.0
36	0.09883634193644637	0.0	0.0	0.0	0.0
37	0.09883634193644637	0.0	0.0	0.0	0.0
38	0.09883634193644637	0.0	0.0	0.0	0.0
39	0.09883634193644637	0.0	0.0	0.0	0.0
40	0.09883634193644637	0.0	0.0	0.0	0.0
41	0.09883634193644637	0.0	0.0	0.0	0.0
42	0.1044308518573773	0.0	0.0	0.0	0.0
43	0.10816052513799791	0.0	0.0	0.0	0.0
44	0.11002536177830823	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCCG	25	1.9285615E-5	50.561554	39
GCACCGA	15	0.008024632	50.56155	40
TTCGCCG	15	0.008176082	50.32328	36
ATATTTT	15	0.008298764	50.134274	47
CTGCAAT	25	2.0280879E-5	50.134274	49
ACCAACA	60	0.0	50.087242	42
CTCAGGC	15	0.008329648	50.087242	42
TCCTCCA	15	0.008391671	49.993446	50
GATATGG	20	4.150557E-4	49.993446	50
TTAAAAG	15	0.008391671	49.993446	33
GAGGAAT	25	2.085194E-5	49.9	20
CTCTCAT	20	4.1891172E-4	49.9	12
CGTGGTA	20	4.1891172E-4	49.9	14
GAATACC	25	2.085194E-5	49.9	23
TTTTGAA	15	0.008454037	49.899998	31
CCGCAGG	15	0.008454037	49.899998	28
TCTACCT	15	0.008454037	49.899998	11
GCGCCAT	15	0.008454037	49.899998	24
AGGCTAT	15	0.008454037	49.899998	20
GAATTCC	15	0.008454037	49.899998	12
>>END_MODULE