FastQCFastQC Report
Fri 17 Jun 2016
SRR1526302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91594
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1280.13974714500949845No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1230.13428827215756492No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1210.1321047230167915No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1180.12882939930563136No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1140.12446230102408455No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1080.11791165360176431No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1070.11681987903137761No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1040.11354455532021747No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1030.11245278074983077No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1020.11136100617944408No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG940.10262680961635041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA1050.050.4753539
TTAATTA150.00809744650.47534641
CGACCAG150.00809744650.47534640
CACCTGA150.00809744650.47534641
CCTCCTT150.00809744650.47534640
ATACCCG150.00809744650.47534639
TAACCAC150.00811520650.44739538
ACAGACC150.00811520650.44739538
TTCATCC150.00818654150.3359137
TTATTTC251.9916573E-550.3359137
TTCACCT150.00824034650.2526236
TGAGTCT150.00824034650.2526236
TATGCCT204.0833023E-450.19724335
AACGATT451.2914825E-1050.16960547
CGATTCA150.00829441550.169649
CACTGAG150.00829441550.169649
GCTGAAT204.0944264E-450.169649
TAATTAT150.00829441550.169642
CTCATCA150.00829441550.169644
ATATGCC402.570232E-950.1419934