FastQCFastQC Report
Fri 17 Jun 2016
SRR1526301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58215
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1350.23189899510435455No Hit
CCAGACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC960.1649059520742077No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG920.15803487073778236No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC810.13913939706261272No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG760.13055054539208108No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG700.1202439233874431No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC690.11852615305333676No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC670.11509061238512411No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC640.10993730138280512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA203.932533E-450.54965238
AGGGATC203.932533E-450.54965238
CCGTACG203.932533E-450.54965240
CCGTCTC150.00803565350.5496540
GATCACC150.00803565350.5496538
TTGGTTT150.00803565350.5496538
CCCCATT150.00803565350.5496540
TGCCTGT150.00809120450.4617437
CAGGCAT203.9665136E-450.4617437
GACCCGT203.9665136E-450.4617437
AAGATCA150.00817506350.3304436
TATGCCT204.0351745E-450.28682735
CTCCACG204.1047763E-450.1131344
GTGCGTA204.1047763E-450.1131344
TCTCAAC204.1047763E-450.1131343
CCTAAAC150.008316264550.11312543
CTTATAT150.008316264550.11312544
CGATTAA252.0458265E-550.0698949
ACTCCTC402.5956979E-950.02672646
ACCAAGC252.0563117E-550.02672648