##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526301_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58215 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.847393283517995 39.0 38.0 39.0 35.0 40.0 2 37.55605943485356 39.0 38.0 39.0 34.0 40.0 3 36.44038478055484 39.0 38.0 39.0 32.0 40.0 4 36.53661427467148 38.0 36.0 39.0 31.0 40.0 5 36.064107188868846 39.0 37.0 39.0 30.0 40.0 6 36.886386670102205 39.0 37.0 39.0 32.0 40.0 7 36.97407884565833 38.0 37.0 39.0 33.0 40.0 8 36.95167912050159 38.0 37.0 39.0 33.0 40.0 9 37.08959890062699 38.0 38.0 39.0 33.0 40.0 10 36.32331873228549 38.0 36.0 39.0 31.0 40.0 11 35.20092759598042 38.0 34.0 39.0 28.0 40.0 12 32.85381774456755 36.0 30.0 39.0 18.0 40.0 13 32.31208451430044 36.0 30.0 39.0 16.0 40.0 14 31.829356695009878 36.0 28.0 39.0 14.0 40.0 15 32.33116894271236 36.0 29.0 39.0 17.0 40.0 16 32.13103152108563 36.0 29.0 39.0 17.0 40.0 17 32.34173323026711 36.0 29.0 38.0 18.0 40.0 18 32.42578373271493 35.0 29.0 38.0 19.0 40.0 19 33.349978527870825 36.0 30.0 38.0 23.0 40.0 20 33.38953877866529 36.0 30.0 39.0 24.0 40.0 21 34.92230524778837 38.0 34.0 39.0 27.0 40.0 22 34.57114145838701 38.0 33.0 39.0 25.0 40.0 23 34.71641329554239 38.0 33.0 39.0 26.0 40.0 24 34.30294597612299 38.0 33.0 39.0 24.0 40.0 25 34.58742592115434 38.0 33.0 39.0 26.0 40.0 26 35.03713819462338 38.0 35.0 39.0 27.0 40.0 27 35.07142489049214 38.0 35.0 39.0 27.0 40.0 28 34.924503993816025 38.0 35.0 39.0 27.0 40.0 29 34.82569784419823 38.0 35.0 39.0 27.0 40.0 30 34.56768874001546 38.0 34.0 39.0 27.0 40.0 31 34.28269346388388 38.0 33.0 39.0 25.0 40.0 32 33.15305333676887 37.0 32.0 39.0 18.0 40.0 33 34.33657991926479 38.0 34.0 39.0 26.0 40.0 34 34.0574765953792 38.0 33.0 39.0 25.0 40.0 35 34.09339517306536 38.0 33.0 39.0 26.0 40.0 36 31.893566950098773 35.0 31.0 38.0 21.0 39.0 37 31.671356179678778 35.0 30.0 38.0 20.0 39.0 38 30.9495490852873 34.0 30.0 38.0 13.0 39.0 39 28.954479086146183 33.0 26.0 37.0 4.0 39.0 40 24.61166366056858 30.0 11.0 36.0 4.0 39.0 41 21.17921497895731 24.0 8.0 32.0 4.0 36.0 42 25.03775659194366 30.0 20.0 33.0 4.0 36.0 43 20.72891866357468 24.0 7.0 32.0 4.0 36.0 44 21.88530447479172 26.0 8.0 33.0 4.0 36.0 45 24.518165421283175 30.0 18.0 33.0 4.0 36.0 46 24.526874516877093 30.0 10.0 35.0 4.0 38.0 47 23.752366228635232 29.0 5.0 35.0 4.0 38.0 48 23.823516275873917 29.0 4.0 35.0 4.0 38.0 49 21.119934724727305 25.0 4.0 35.0 4.0 38.0 50 23.620905264966073 29.0 4.0 35.0 4.0 38.0 51 25.201872369664176 32.0 4.0 37.0 4.0 39.0 52 25.00322940822812 31.0 4.0 37.0 4.0 39.0 53 24.60886369492399 31.0 4.0 37.0 4.0 39.0 54 23.684462767328007 30.0 4.0 37.0 4.0 39.0 55 23.934656016490596 31.0 4.0 37.0 4.0 39.0 56 23.27492914197372 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.33103815363331535 1 2 0.7778832815902064 1 3 2.2870401962615006 1 4 1.7745372124342396 1 5 2.5571216418405385 1 6 1.3016490172233048 1 7 1.0379114220406578 1 8 1.1365840470751394 1 9 1.1865551028462136 1 10 1.9535568456011703 1 11 -4.23601893693996 1 12 -7.59054251820055 1 13 -20.152303385811734 1 14 -18.019189946114373 1 15 -11.941082153051713 1 16 -21.049452558556574 1 17 -21.056183788872843 1 18 -20.726143545522497 1 19 -5.853377177385092 1 20 -10.644194208186729 1 21 -6.758909006739859 1 22 -2.989218035791083 1 23 -3.8396287963206923 1 24 -3.5395920060615573 1 25 -3.2761348658999623 1 26 2.7714630352518626 1 27 2.83894564548482 1 28 2.533474498194458 1 29 1.7996887206030223 1 30 2.2458771922845457 1 31 1.5590694463223045 1 32 -11.042348635769347 1 33 1.5799597564711902 1 34 1.712214604435637 1 35 1.901147686584352 1 36 4.874902865069192 1 37 4.852047694450874 1 38 5.512898909814652 1 39 8.008010931326286 1 40 10.629178192378966 1 41 -2.311930945844903 1 42 -6.675339325796195 1 43 -12.395027195929984 1 44 -14.315934711301981 1 45 -13.744756323776022 1 46 -17.426953188534323 1 47 -16.749973583823063 1 48 -15.42363322257479 1 49 -13.027404819456674 1 50 -15.238262260406671 1 51 -17.238567672908516 1 52 -17.26790122825924 1 53 -17.262722615237706 1 54 -16.98337074532171 1 55 -16.62043220071747 1 56 -16.210558828694726 2 1 1.0490294381321732 2 2 1.528661451334969 2 3 2.818893277813693 2 4 2.4300674054203313 2 5 2.99372674663406 2 6 2.2514311296947653 2 7 1.920667180496764 2 8 1.986552920285348 2 9 1.7787941565918004 2 10 2.5287799209280024 2 11 -3.696072890042263 2 12 -10.560453288069816 2 13 -13.5845507400346 2 14 -15.411802521337451 2 15 -9.24093689469936 2 16 -3.451610682648713 2 17 -2.221155774762032 2 18 -9.47007382151337 2 19 -2.5912896073498146 2 20 -2.6786411888881183 2 21 3.357920210940158 2 22 3.932342453937075 2 23 3.2693149679457534 2 24 3.1547429181965896 2 25 3.543537265247579 2 26 2.549632780012182 2 27 3.142743113839252 2 28 2.9194466915955744 2 29 3.2228055498206984 2 30 2.9377219733594586 2 31 0.5057388798147286 2 32 -6.561128465609563 2 33 0.22200167388143655 2 34 1.1444619586585105 2 35 0.40166646641419135 2 36 5.147158519769338 2 37 5.128246075857803 2 38 6.417028397260182 2 39 8.387757766769322 2 40 10.07553635797349 2 41 4.7764691371598715 2 42 0.07481630815275864 2 43 -2.9787582604661935 2 44 -9.031643364549543 2 45 -3.2701765354381926 2 46 -12.118382945745363 2 47 -12.890251649811857 2 48 -10.277544822491784 2 49 -11.766977344876885 2 50 -10.419835200850747 2 51 -11.125681181935285 2 52 -11.007473753679452 2 53 -11.194866213494608 2 54 -14.824209093526731 2 55 -10.897253117920208 2 56 -10.150795392297132 3 1 1.2492472140951492 3 2 1.7718899799861134 3 3 3.0495996885936023 3 4 2.366640156202969 3 5 3.2618634010508316 3 6 2.4289185395219093 3 7 1.8047014802109373 3 8 2.096393671612425 3 9 2.0689806272601032 3 10 2.4028781923312934 3 11 1.8255005412902534 3 12 2.260153762426981 3 13 -4.413188279166221 3 14 -4.534437610489487 3 15 -2.7642797557310708 3 16 3.8199287211895907 3 17 3.8165466888285486 3 18 3.0464635406752762 3 19 2.0011971219274365 3 20 2.1365214625342333 3 21 3.7556335633288853 3 22 4.440713217514045 3 23 3.9557175812573107 3 24 4.6188779392936326 3 25 3.704827587828227 3 26 3.283537775248334 3 27 2.892709086345036 3 28 3.2167108810606635 3 29 3.1961279943558836 3 30 3.178364412450243 3 31 4.344720777187803 3 32 3.0642968780681272 3 33 3.777738709406144 3 34 3.8870779924137793 3 35 3.3666862023608317 3 36 5.955107342418035 3 37 6.797226612131112 3 38 6.694284420126657 3 39 8.749946415197254 3 40 11.475291360015142 3 41 9.850240744618699 3 42 7.579375992377614 3 43 2.7079165727990855 3 44 4.003473009480672 3 45 7.658910166617069 3 46 1.3054635222007391 3 47 -3.6380398626878616 3 48 0.4290020673952455 3 49 -7.972231189983731 3 50 0.27487012104935005 3 51 -0.07287051091309849 3 52 -0.6213297493239338 3 53 -0.47566109575947735 3 54 -3.659379911547692 3 55 -0.18662292872734199 3 56 3.3528115220896915 4 1 1.6581480504153419 4 2 2.09148376732184 4 3 3.380543535666483 4 4 2.586771577947289 4 5 2.9825921943602722 4 6 2.692359399736965 4 7 1.4955616952646977 4 8 1.9132992869051364 4 9 2.0080487276185224 4 10 2.5892581206467113 4 11 3.8652257503703 4 12 4.634108362188037 4 13 5.7746850780619745 4 14 3.952121529295461 4 15 6.826222036383623 4 16 7.389223822742757 4 17 6.877179902687569 4 18 6.129199502443498 4 19 5.065414565177139 4 20 5.204621820957172 4 21 3.355693300485399 4 22 4.722075224682506 4 23 3.9348095764783366 4 24 4.109618680034373 4 25 3.77501874672906 4 26 3.1682450631814305 4 27 3.246353052892225 4 28 2.958945050714192 4 29 3.184479248836169 4 30 2.9397144721874042 4 31 3.6213038118353538 4 32 4.504858407339334 4 33 3.3700326162161787 4 34 3.31300391833971 4 35 3.2274986276893856 4 36 5.338321201438351 4 37 5.490177627662774 4 38 6.3050968454552105 4 39 8.87838130169666 4 40 11.014120511747514 4 41 6.335306455490862 4 42 7.996640030609392 4 43 6.1171160949018315 4 44 11.265121755000386 4 45 8.958492006521489 4 46 7.843993988150558 4 47 8.171398608040931 4 48 7.865680681493213 4 49 4.661878726384252 4 50 7.816686130607295 4 51 5.108730444881406 4 52 5.393604539803906 4 53 4.822128629449601 4 54 -0.007050162443750452 4 55 4.3868537737816204 4 56 5.25543995697619 5 1 1.263787781055072 5 2 1.6070561578942275 5 3 1.9293650844880332 5 4 1.6629500291257386 5 5 2.8041410159090887 5 6 2.1203897027672625 5 7 0.7493495740525802 5 8 1.4743262229320706 5 9 1.3590588286286263 5 10 1.728147009535597 5 11 2.9228856830302306 5 12 5.512475365555041 5 13 6.669045347422244 5 14 5.963485165659094 5 15 6.013090723252308 5 16 6.931732240251172 5 17 5.852348252855922 5 18 5.107313980557976 5 19 4.709774834537409 5 20 5.173056164391511 5 21 3.2336394284315304 5 22 3.7915196691269486 5 23 3.2786647953335546 5 24 3.509029454445148 5 25 3.770809992520306 5 26 1.9102484301847937 5 27 2.395763827303 5 28 2.2236756904448285 5 29 2.511499450856377 5 30 2.7115999940729267 5 31 2.595209535741077 5 32 3.6454307979117218 5 33 2.8081639293474936 5 34 1.6181385984743883 5 35 1.950141725332486 5 36 3.014247127364275 5 37 3.5251102875954388 5 38 3.576982367340733 5 39 5.098920022235379 5 40 4.08819458582159 5 41 1.148016893201298 5 42 6.226858885828246 5 43 11.074607677393416 5 44 10.123707613586244 5 45 7.968172141201624 5 46 8.78970105885763 5 47 9.140674702317028 5 48 8.534451725264258 5 49 9.203966268471792 5 50 8.000187814108976 5 51 7.6179897041406655 5 52 7.1793789505783145 5 53 7.482061289382262 5 54 7.645727615334028 5 55 7.040894177822025 5 56 7.722190798727034 6 1 1.1488887911560823 6 2 1.639631915469991 6 3 2.9435064986294464 6 4 1.3960308372065526 6 5 3.006666268434344 6 6 1.9145816219591865 6 7 1.3455616952646992 6 8 1.4892252128310588 6 9 1.774715394285188 6 10 2.311480342868933 6 11 3.2781887133332646 6 12 5.452626880706553 6 13 6.517277670654568 6 14 7.502121529295458 6 15 6.465110925272516 6 16 7.07440900792794 6 17 5.853105828613497 6 18 6.09771802096202 6 19 4.596896046658625 6 20 5.182399598734946 6 21 3.1649525597446626 6 22 3.5442974469047286 6 23 3.0496243912931504 6 24 3.1299890504047454 6 25 3.165759487469799 6 26 2.1756524705888367 6 27 2.83894564548482 6 28 1.8607969025660438 6 29 1.9789236932806133 6 30 2.2156403981133295 6 31 2.9472297377612833 6 32 4.380784333265261 6 33 2.6311437273272915 6 34 3.020411325747112 6 35 3.320091220281981 6 36 4.214247127364278 6 37 3.786473923959072 6 38 4.647689438047799 6 39 5.830233153548509 6 40 2.8437501413771464 6 41 3.476047196231601 6 42 7.939232623201985 6 43 12.976375354161092 6 44 11.631788421667054 6 45 8.94923274726223 6 46 11.816216210372783 6 47 11.556583793226121 6 48 10.006421422233956 6 49 11.915582430087955 6 50 10.175945389866552 6 51 9.101323037474 6 52 9.886197132396497 6 53 8.457313814634787 6 54 9.892949837556252 6 55 9.142409329337177 6 56 9.251736253272487 7 1 1.3644175729499253 7 2 2.0609170560322383 7 3 2.999463660610701 7 4 1.9347189228825883 7 5 3.5563315964129245 7 6 1.9795079941144706 7 7 1.8078106912486334 7 8 1.9098410093504725 7 9 2.178062114499383 7 10 2.526339780619935 7 11 4.019955781606356 7 12 6.590377884722621 7 13 6.8214276037201635 7 14 8.123808276283413 7 15 6.823879332233691 7 16 7.081102448356319 7 17 6.796077716163698 7 18 6.681519895125341 7 19 5.395289620955811 7 20 5.768209505026782 7 21 2.8415255182453265 7 22 3.0761582233438176 7 23 3.327268300262361 7 24 3.651006453349858 7 25 2.8567902772957723 7 26 1.7329483206557725 7 27 2.754608296087227 7 28 1.9905157780680511 7 29 1.97330120332078 7 30 2.369321790348941 7 31 2.9843113977345084 7 32 4.236339888820815 7 33 2.444664476992614 7 34 3.0035438558675978 7 35 2.519555745047711 7 36 1.646777247846206 7 37 1.5937028396217237 7 38 2.44554753711072 7 39 4.718854304820262 7 40 -0.887709028636241 7 41 -8.482185735495307 7 42 4.880062609815106 7 43 9.702345903023208 7 44 8.559633133849115 7 45 5.591803028386728 7 46 8.32023227462981 7 47 9.314415118527322 7 48 8.134533872033153 7 49 11.558018842403886 7 50 8.535784747296272 7 51 10.187266812574403 7 52 9.184992313119391 7 53 9.121436973938671 7 54 9.80687219364728 7 55 10.825943465883359 7 56 9.545712156886946 8 1 1.478390706864893 8 2 1.705149156849295 8 3 3.082970100162008 8 4 2.6228507605781957 8 5 3.2511107128787913 8 6 2.180865146863397 8 7 1.7398145688279172 8 8 1.4608727224096043 8 9 1.793489340645344 8 10 2.271250457811462 8 11 3.244855379999933 8 12 6.727339524384718 8 13 6.7303044905779394 8 14 6.943500839640286 8 15 7.078137745195885 8 16 7.0935660960505444 8 17 6.857703529762919 8 18 6.014576258509912 8 19 5.521033977693104 8 20 5.4383382960529545 8 21 3.2116958547638177 8 22 3.884910473724652 8 23 2.77069718822802 8 24 3.124625065730413 8 25 3.6814682997303407 8 26 1.4396371449183363 8 27 2.5171065650250526 8 28 2.1929808106120205 8 29 2.6892685208668254 8 30 3.3819239230175455 8 31 2.5912910404432736 8 32 3.868523796866789 8 33 2.7617950683234582 8 34 2.5537446590804507 8 35 3.7415471589793015 8 36 0.5832126446056556 8 37 0.9703819699360849 8 38 0.9771913537566128 8 39 1.9850224255791602 8 40 -2.716403115327836 8 41 -10.314757401469548 8 42 3.9216081021291878 8 43 8.987486465272202 8 44 8.016079609406514 8 45 5.443485620825449 8 46 8.086331152901515 8 47 8.051986092076692 8 48 7.750099583153496 8 49 10.657728023957684 8 50 8.265600562280348 8 51 9.390978209887795 8 52 9.934472994465466 8 53 9.707505385516015 8 54 10.35693451188575 8 55 9.448156455773958 8 56 8.994264988904671 9 1 1.676666568933861 9 2 2.1443938202318904 9 3 3.0300144351373817 9 4 2.37777686895258 9 5 3.2034916652597403 9 6 2.632835590213155 9 7 1.805085504788508 9 8 1.8590664826723327 9 9 1.7540804736502693 9 10 2.668623200011787 9 11 4.006760141904692 9 12 6.402626880706556 9 13 6.60140465478155 9 14 7.868788195962125 9 15 7.318285528447117 9 16 7.459329642848573 9 17 7.374534400042069 9 18 6.84216246540646 9 19 5.54689604665862 9 20 5.426844043179393 9 21 3.952254147046247 9 22 4.142710145317423 9 23 3.8527989944677543 9 24 4.547449367865063 9 25 3.4244896461999588 9 26 2.817715962652329 9 27 3.077040883580061 9 28 2.9655588073279446 9 29 3.6646379789949037 9 30 2.9291324616053913 9 31 3.6980233885549296 9 32 4.7482446507255744 9 33 3.437492933676495 9 34 3.5537446590804507 9 35 4.038345188535949 9 36 3.133294746411895 9 37 3.048378685863831 9 38 3.461181501539862 9 39 6.36515378846914 9 40 -3.745138747511742 9 41 -2.9358575656731603 9 42 6.557486591455955 9 43 10.975581703367443 9 44 9.273058262936893 9 45 6.68732798535747 9 46 10.542406686563258 9 47 11.082774269416593 9 48 11.451659517472052 9 49 13.63145544596097 9 50 11.028326342247507 9 51 10.451323037474001 9 52 10.569530465729834 9 53 10.963663020983994 9 54 9.949299043905455 9 55 9.859075996003842 9 56 9.099355300891535 10 1 1.7291357047363292 10 2 2.0482738907786313 10 3 2.899062054185002 10 4 2.7812160223917317 10 5 3.2264193548540945 10 6 2.3528532268974587 10 7 2.010993794030135 10 8 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6.390149760334165 116 42 3.3029078368772 116 43 -9.768923791138054 116 44 -0.051117561238932296 116 45 0.9620532600827438 116 46 -4.844040199883629 116 47 -8.374185437543112 116 48 -6.0922965264839934 116 49 -5.90792184341632 116 50 -4.783028969107807 116 51 -2.8435487573977944 116 52 -2.5714951752958086 116 53 -2.8046519973310247 116 54 -8.344657000050587 116 55 -4.390924003996158 116 56 -2.4290329774967425 117 1 -0.19977021267533956 117 2 0.21089628328608256 117 3 1.4622804449895952 117 4 0.1860691513827959 117 5 1.8488118623040748 117 6 0.8590260664036293 117 7 0.6478605458394142 117 8 0.4723669752831725 117 9 0.42567324869131795 117 10 0.9609056302252554 117 11 -12.19192622919547 117 12 -12.387603004350918 117 13 -10.798431141605967 117 14 -7.401326746566607 117 15 -5.335655358252392 117 16 -12.837468386708075 117 17 -12.372181527708342 117 18 -3.8589869598808946 117 19 -3.6283913096632148 117 20 -4.940972048774633 117 21 -0.5124420762706663 117 22 -0.3334803308730514 117 23 0.40288109627399393 117 24 -4.082271485993722 117 25 0.16422692041999198 117 26 1.4396371449183363 117 27 1.8044628868641297 117 28 1.9975785117614464 117 29 1.9421420840852122 117 30 0.876176796580765 117 31 -0.7880193043843136 117 32 -11.453315283592978 117 33 0.2560479418866777 117 34 -1.055450743218401 117 35 -1.2469585881471374 117 36 1.03148850667462 117 37 1.9588877170625167 117 38 2.2070764112278773 117 39 3.6976661037400795 117 40 -0.8428398969370292 117 41 -5.1078608497454105 117 42 -5.457702242698399 117 43 -9.897571005992164 117 44 -6.167828436570495 117 45 -4.76341093089869 117 46 -6.672289536753656 117 47 -9.591692068842848 117 48 -9.05449811799593 117 49 -7.342271976042314 117 50 -8.194169552662183 117 51 -6.080286157928299 117 52 -6.306906315879363 117 53 -6.683299212185135 117 54 -9.40168617776942 117 55 -7.4254067626168485 117 56 -3.913780988106822 118 1 -0.13837408147264796 118 2 0.365648175632586 118 3 1.7120701842663024 118 4 0.6881717586157663 118 5 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0.6288950285980306 120 8 0.9447807683866145 120 9 0.7886042831740809 120 10 1.6281470095355957 120 11 -11.963477953333403 120 12 -2.2557064526267787 120 13 -9.235500107123208 120 14 -3.8978784707045406 120 15 -6.146000185838599 120 16 -9.647813214294281 120 17 -9.56356083805317 120 18 -3.574504201260204 120 19 -2.7697706200080425 120 20 -3.806489290153941 120 21 0.8177303375224341 120 22 1.1165196691269514 120 23 0.6468466135153719 120 24 -5.122788727373031 120 25 -0.4814627347524194 120 26 1.6034302483666139 120 27 2.3889456454848172 120 28 2.2691302358993752 120 29 2.6455903599472848 120 30 2.043418175891105 120 31 -2.513881373349829 120 32 -12.863660111179186 120 33 -2.4577451615616006 120 34 -12.804588674252884 120 35 -3.9521310019402414 120 36 -2.0857528726357266 120 37 -1.8885260760409288 120 38 -1.8411994508410885 120 39 -0.3419890686737155 120 40 -3.66180541417841 120 41 -1.1656194704350646 120 42 -5.21632293235357 120 43 -3.4958468680611308 120 44 -4.5376560227773926 120 45 -12.859100586071103 120 46 -14.042117122960553 120 47 -13.793416206773882 120 48 -13.601911911099378 120 49 -13.473306458800934 120 50 -13.040721276800113 120 51 -13.232010295859334 120 52 -13.763802867603502 120 53 -13.281575074254102 120 54 -14.004272384665972 120 55 -14.365924003996158 120 56 -11.123263746727513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 415.0 5 38.0 6 37.0 7 55.0 8 135.0 9 165.0 10 109.0 11 75.0 12 106.0 13 106.0 14 146.0 15 228.0 16 354.0 17 434.0 18 541.0 19 688.0 20 833.0 21 992.0 22 1259.0 23 1522.0 24 1721.0 25 1780.0 26 1924.0 27 2046.0 28 2164.0 29 2182.0 30 2629.0 31 2917.0 32 3409.0 33 4328.0 34 4969.0 35 5769.0 36 6241.0 37 4953.0 38 2357.0 39 588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.55166194279825 0.3366829854848407 0.06183973202782787 0.049815339689083575 8 99.38675599072404 0.43287812419479516 0.13055054539208108 0.049815339689083575 9 96.8007422552877 1.8040927131836224 0.6357279084551812 0.7594371230734867 10 46.57562483895903 30.90612385124109 11.150047238684188 11.368204071115692 11 32.71321824272095 29.495834406939792 17.710212144636262 20.080735205703 12 35.06484583011251 12.639354118354376 20.810787597698187 31.48501245383492 13 29.29141973718114 12.16009619513871 22.61616421884394 35.93231984883621 14 23.198116935553152 14.660756318402832 26.289022902599523 35.852103843444496 15 26.834471723758234 19.291846601173372 25.04860382292724 28.825077852141153 16 31.970649895178198 16.082413994569887 24.710451249269685 27.23648486098223 17 29.183561267266857 24.17016012645179 22.251048037935536 24.39523056834582 18 30.58580999828208 23.15753306991926 22.422264215770486 23.834392716028173 19 33.41980483782298 24.517248488180318 18.774051676745465 23.288894997251237 20 29.126430461527885 27.289597580672876 20.56943537578611 23.01453658201313 21 27.00005153665115 21.877308412499357 26.598065657693564 24.52457439315593 22 27.560380664444978 20.44010031951077 26.716940942041433 25.282578074002814 23 30.701709181482435 21.807094391479858 24.205101777892295 23.286094649145408 24 27.711071029803314 24.701537404449027 23.64682641930774 23.940565146439923 25 30.682813707807266 20.932749291419736 22.42892725242635 25.955509748346646 26 22.55821558267215 20.55143452145328 30.175722423971223 26.714627471903345 27 20.872568561413154 17.13004330194515 30.23919169702385 31.75819643961784 28 22.22336947169162 23.154362416107382 29.977628635346754 24.64463947685424 29 26.528403037905086 22.24131413450634 29.251864325234543 21.97841850235403 30 22.152725273214653 22.95003092996082 35.24812701903911 19.649116777785412 31 25.353858869717943 20.141543547887178 35.31356545097634 19.191032131418535 32 29.85138733785757 16.89373765140452 33.33390602181943 19.920968988918478 33 24.621844401232124 17.91399218736556 35.86240126654162 21.601762144860697 34 20.79630043493957 22.642644707662157 40.23620828963881 16.32484656775946 35 20.80089370112572 21.91802010827533 41.259774856062556 16.021311334536392 36 21.73643730965777 25.002150759648305 36.5405461208899 16.72086580980402 37 22.227388578907487 22.752634841909487 37.0720534545705 17.947923124612522 38 24.955264969029596 25.385409497591187 34.73158981417757 14.927735719201651 39 21.586579078949637 18.334798875842687 31.65744236969603 28.42117967551165 40 14.931125667571166 14.39361205689694 26.365820356383622 44.30944191914827 41 16.700752192450345 15.575929841588962 28.89354916981525 38.829768796145444 42 19.259170008778423 22.47448233127356 37.97614334647228 20.290204313475737 43 11.02259010174168 12.605621658906706 31.814105880324195 44.55768235902742 44 14.200631829716711 13.974485127833308 26.89246810640979 44.93241493604019 45 18.199325950890707 20.960863883348235 39.35793383313845 21.4818763326226 46 20.786053228801322 20.24963895192903 38.369094285124824 20.595213534144833 47 16.410009624639077 20.9679637013612 36.829712635776154 25.79231403822357 48 18.782223138791505 22.11452533168351 40.915652711899355 18.187598817625627 49 14.85107556536128 14.285714285714285 27.321773303916164 43.54143684500828 50 21.080178365442556 19.899109894461375 40.30955701323967 18.711154726856392 51 20.87103390034986 21.277769160505315 38.667424986643226 19.183771952501594 52 17.331930961946306 21.054537303653635 40.7659016845699 20.847630049830162 53 17.519705366055142 22.07173097442605 42.12301655594947 18.28554710356934 54 15.413727851367623 20.698434543265094 35.71477722346465 28.17306038190263 55 17.295548787831862 22.27498752559404 40.00068824308746 20.42877544348664 56 20.97953845359583 22.27365812524738 36.08046946256174 20.666333958595054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.5 11 2.0 12 3.5 13 12.0 14 19.0 15 36.0 16 53.0 17 65.0 18 77.0 19 118.0 20 159.0 21 219.5 22 349.5 23 419.0 24 522.5 25 626.0 26 784.0 27 942.0 28 1153.0 29 1611.5 30 1859.0 31 2036.5 32 2214.0 33 2195.0 34 2176.0 35 2092.5 36 2009.0 37 1804.0 38 1397.5 39 1196.0 40 1095.5 41 995.0 42 922.5 43 850.0 44 870.0 45 890.0 46 896.5 47 1080.0 48 1257.0 49 1449.0 50 1641.0 51 1675.0 52 1709.0 53 1827.0 54 2083.5 55 2222.0 56 2455.5 57 2689.0 58 2915.5 59 3142.0 60 3296.5 61 3451.0 62 3823.0 63 4179.0 64 4163.0 65 3841.5 66 3520.0 67 3181.5 68 2843.0 69 2495.0 70 2147.0 71 1867.0 72 1370.0 73 1153.0 74 949.5 75 746.0 76 637.5 77 529.0 78 411.5 79 237.0 80 180.0 81 138.5 82 97.0 83 71.5 84 46.0 85 28.5 86 11.0 87 9.5 88 4.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.024048784677488617 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02061324400927596 15 0.15803487073778236 16 0.03607317701623293 17 0.018895473675169628 18 0.00858885167053165 19 0.012024392338744309 20 0.029202095679807607 21 0.00687108133642532 22 0.00171777033410633 23 0.0 24 0.0 25 0.0 26 0.19239027741990894 27 0.03263763634802027 28 0.18036588508116463 29 0.029202095679807607 30 0.03263763634802027 31 0.24220561710899252 32 0.0171777033410633 33 0.17864811474705833 34 0.07901743536889118 35 0.0515331100231899 36 0.1649059520742077 37 0.2525122391136305 38 0.16318818174010136 39 0.37103839216696727 40 0.6115262389418534 41 0.8880872627329726 42 0.20269689942454694 43 0.38649832517392424 44 0.494717856222623 45 0.10134844971227347 46 0.08760628703942283 47 0.05325088035729623 48 0.04637979902087091 49 0.34183629648715963 50 0.22674568410203555 51 0.3298119041484154 52 0.37447393283517993 53 0.18723696641758997 54 0.14601047839903805 55 0.1649059520742077 56 0.18208365541527097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 58215.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.38323456153913 #Duplication Level Percentage of deduplicated Percentage of total 1 93.55512249443207 80.81594090870051 2 4.201797645561565 7.2592974319333505 3 0.9723989818644607 2.519969080133986 4 0.4215717467387846 1.4566692433221677 5 0.25851097677378304 1.1165507171691145 6 0.13919821826280623 0.7214635403246585 7 0.08351893095768374 0.505024478227261 8 0.06959910913140312 0.4809756935497724 9 0.03977091950365892 0.3091986601391394 >10 0.23464842507158767 3.244868161126857 >50 0.02187400572701241 1.3381430902688312 >100 0.001988545975182946 0.23189899510435455 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 135 0.23189899510435455 No Hit CCAGACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 96 0.1649059520742077 No Hit CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 92 0.15803487073778236 No Hit CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 81 0.13913939706261272 No Hit CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 76 0.13055054539208108 No Hit CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 70 0.1202439233874431 No Hit CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 69 0.11852615305333676 No Hit CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 67 0.11509061238512411 No Hit CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 64 0.10993730138280512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.00171777033410633 0.0 0.0 0.0 0.0 11 0.00171777033410633 0.0 0.0 0.0 0.0 12 0.00515331100231899 0.0 0.0 0.0 0.0 13 0.00687108133642532 0.0 0.0 0.0 0.0 14 0.008588851670531649 0.0 0.0 0.0 0.0 15 0.01374216267285064 0.0 0.0 0.0 0.0 16 0.017177703341063298 0.0 0.0 0.0 0.0 17 0.02061324400927596 0.0 0.0 0.0 0.0 18 0.03091986601391394 0.0 0.0 0.0 0.0 19 0.034355406682126596 0.0 0.0 0.0 0.0 20 0.034355406682126596 0.0 0.0 0.0 0.0 21 0.03607317701623293 0.0 0.0 0.0 0.0 22 0.03607317701623293 0.0 0.0 0.0 0.0 23 0.03607317701623293 0.0 0.0 0.0 0.0 24 0.04122648801855192 0.0 0.0 0.0 0.0 25 0.04466202868676458 0.0 0.0 0.0 0.0 26 0.04809756935497724 0.0 0.0 0.0 0.0 27 0.04981533968908357 0.0 0.0 0.0 0.0 28 0.0515331100231899 0.0 0.0 0.0 0.0 29 0.0515331100231899 0.0 0.0 0.0 0.0 30 0.0515331100231899 0.0 0.0 0.0 0.0 31 0.05325088035729623 0.0 0.0 0.0 0.0 32 0.05325088035729623 0.0 0.0 0.0 0.0 33 0.05325088035729623 0.0 0.0 0.0 0.0 34 0.05325088035729623 0.0 0.0 0.0 0.0 35 0.05325088035729623 0.0 0.0 0.0 0.0 36 0.05325088035729623 0.0 0.0 0.0 0.0 37 0.05325088035729623 0.0 0.0 0.0 0.0 38 0.05325088035729623 0.0 0.0 0.0 0.0 39 0.05325088035729623 0.0 0.0 0.0 0.0 40 0.05325088035729623 0.0 0.0 0.0 0.0 41 0.05325088035729623 0.0 0.0 0.0 0.0 42 0.05325088035729623 0.0 0.0 0.0 0.0 43 0.05325088035729623 0.0 0.0 0.0 0.0 44 0.06012196169372155 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCGTA 20 3.932533E-4 50.549652 38 AGGGATC 20 3.932533E-4 50.549652 38 CCGTACG 20 3.932533E-4 50.549652 40 CCGTCTC 15 0.008035653 50.54965 40 GATCACC 15 0.008035653 50.54965 38 TTGGTTT 15 0.008035653 50.54965 38 CCCCATT 15 0.008035653 50.54965 40 TGCCTGT 15 0.008091204 50.46174 37 CAGGCAT 20 3.9665136E-4 50.46174 37 GACCCGT 20 3.9665136E-4 50.46174 37 AAGATCA 15 0.008175063 50.33044 36 TATGCCT 20 4.0351745E-4 50.286827 35 CTCCACG 20 4.1047763E-4 50.11313 44 GTGCGTA 20 4.1047763E-4 50.11313 44 TCTCAAC 20 4.1047763E-4 50.11313 43 CCTAAAC 15 0.0083162645 50.113125 43 CTTATAT 15 0.0083162645 50.113125 44 CGATTAA 25 2.0458265E-5 50.06989 49 ACTCCTC 40 2.5956979E-9 50.026726 46 ACCAAGC 25 2.0563117E-5 50.026726 48 >>END_MODULE