FastQCFastQC Report
Fri 17 Jun 2016
SRR1526300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67530
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA1460.2162002073152673No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1210.17917962387087222No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA1050.15548645046645934No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG960.14215904042647715No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG940.13919739375092552No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACCAAAAAAAAAAAAAAA930.13771657041314972No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA680.10069598696875463No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAACCAAAAAAAAAAAAAAA680.10069598696875463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC505.456968E-1250.6470640
GCTTGTC505.456968E-1250.6470638
CATGTCT150.00798017150.64705739
TGCCTCA203.914329E-450.6088941
GATCCTC150.008051855550.5327337
GACCACC203.94357E-450.5327337
TCTTAGT203.987756E-450.4189236
ATGCTTG750.050.4189236
ATGCTGG150.00812401850.4189236
AGACCAC150.00812401850.4189236
ATGCATG150.00812401850.4189236
TATGCTT750.050.38109635
TATGCAT150.00814817950.38109235
AGAGTCC150.00814817950.38109235
CGATTAA402.5138434E-950.23036249
TTAGAGC150.00826978750.19282534
ACTCTTA150.00826978750.19282534
ATATGCC150.00826978750.19282534
CCCACAC150.00826978750.19282543
CCATATC150.00826978750.19282543