##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67530
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.654331408263	38.0	38.0	39.0	35.0	40.0
2	36.26706648896786	38.0	35.0	39.0	31.0	40.0
3	37.03317044276618	38.0	38.0	39.0	33.0	40.0
4	36.926417888345924	38.0	37.0	39.0	33.0	40.0
5	35.65045165111802	38.0	35.0	39.0	29.0	40.0
6	36.800621945801865	38.0	36.0	39.0	33.0	40.0
7	36.81907300459055	38.0	37.0	39.0	33.0	40.0
8	35.65840367244188	38.0	35.0	39.0	30.0	40.0
9	35.66121723678365	38.0	35.0	39.0	30.0	40.0
10	35.24791944321043	37.0	35.0	39.0	29.0	40.0
11	33.970842588479194	36.0	31.0	39.0	26.0	40.0
12	32.47678069006368	36.0	30.0	39.0	18.0	40.0
13	32.33790907744706	36.0	30.0	39.0	17.0	40.0
14	31.79662372278987	36.0	28.0	39.0	14.0	40.0
15	32.42364874870428	36.0	30.0	39.0	17.0	40.0
16	32.098682067229376	36.0	29.0	38.0	17.0	40.0
17	32.28827187916482	36.0	29.0	38.0	18.0	40.0
18	32.48123796831038	36.0	30.0	38.0	19.0	40.0
19	33.299851917666224	36.0	31.0	38.0	23.0	40.0
20	33.20753739078928	36.0	30.0	38.0	23.0	40.0
21	34.737760995113284	38.0	33.0	39.0	26.0	40.0
22	34.4837553679846	38.0	33.0	39.0	25.0	40.0
23	34.623026802902416	38.0	33.0	39.0	26.0	40.0
24	34.26742188656893	38.0	33.0	39.0	24.0	40.0
25	34.46420849992596	38.0	33.0	39.0	26.0	40.0
26	34.80564193691693	38.0	35.0	39.0	27.0	40.0
27	34.85455353176366	38.0	35.0	39.0	27.0	40.0
28	34.73941951725159	38.0	34.0	39.0	27.0	40.0
29	34.43659114467644	38.0	34.0	39.0	26.0	40.0
30	34.31381608174145	38.0	33.0	39.0	25.0	40.0
31	34.00630830741893	38.0	33.0	39.0	24.0	40.0
32	33.059025618243744	37.0	32.0	39.0	17.0	40.0
33	34.19463941951725	38.0	34.0	39.0	25.0	40.0
34	33.93278542869836	38.0	33.0	39.0	24.0	40.0
35	33.85502739523175	38.0	33.0	39.0	24.0	40.0
36	31.612720272471496	35.0	30.0	38.0	18.0	39.0
37	31.367051680734487	35.0	30.0	38.0	16.0	39.0
38	30.752139789723085	35.0	30.0	38.0	10.0	39.0
39	28.74226269806012	33.0	26.0	37.0	4.0	39.0
40	24.632874278098623	30.0	10.0	36.0	4.0	39.0
41	21.10139197393751	24.0	8.0	32.0	4.0	36.0
42	24.917221975418332	30.0	19.0	33.0	4.0	36.0
43	20.778024581667406	24.0	7.0	32.0	4.0	36.0
44	22.08123796831038	27.0	8.0	33.0	4.0	36.0
45	24.537020583444395	29.0	18.0	33.0	4.0	36.0
46	24.63004590552347	30.0	10.0	35.0	4.0	38.0
47	23.908810898859766	29.0	5.0	35.0	4.0	38.0
48	23.794269213682806	29.0	4.0	35.0	4.0	38.0
49	21.325085147341923	26.0	4.0	35.0	4.0	38.0
50	23.504857100547905	29.0	4.0	35.0	4.0	38.0
51	25.08624315119206	32.0	4.0	37.0	4.0	39.0
52	24.83309640159929	31.0	4.0	37.0	4.0	39.0
53	24.431778468828668	31.0	4.0	37.0	4.0	39.0
54	23.629542425588628	30.0	4.0	36.0	4.0	39.0
55	23.686554124092996	30.0	4.0	36.0	4.0	39.0
56	23.395468680586404	31.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.6540446043787611
1	2	1.6365710248997587
1	3	1.496010036467574
1	4	1.4497529220137508
1	5	1.7801129562986375
1	6	1.5480140938201714
1	7	1.7918001428628045
1	8	2.04914287487874
1	9	1.9300290052757205
1	10	1.5368284701232966
1	11	-5.57289214318773
1	12	-8.551938158873895
1	13	-21.520995095032852
1	14	-18.635290012129026
1	15	-13.145307653657131
1	16	-21.76289802010981
1	17	-22.019041908028655
1	18	-20.668349038195586
1	19	-8.328517267083257
1	20	-12.016581027173196
1	21	-9.904122687700198
1	22	-4.8361154028756985
1	23	-4.494779624023778
1	24	-5.037121688555487
1	25	-5.020745663530157
1	26	2.027399491226923
1	27	1.7739794596678848
1	28	2.010189560434874
1	29	1.9963422101106332
1	30	1.7353877625037413
1	31	0.3141800732553648
1	32	-13.815347941920258
1	33	-0.10810101392391402
1	34	0.14399398105508254
1	35	0.353925145981826
1	36	3.8016678868493834
1	37	4.020440899176737
1	38	4.240548019093797
1	39	5.988061299559444
1	40	8.729532685259848
1	41	-9.149247753102433
1	42	-15.417859904346845
1	43	-14.04056365991123
1	44	-15.978624338782733
1	45	-17.348259053410587
1	46	-18.385384220053034
1	47	-17.911940719487422
1	48	-17.51306398287812
1	49	-15.03716195221138
1	50	-17.20267829013271
1	51	-18.650966591513832
1	52	-18.535792759996323
1	53	-18.081728197053124
1	54	-17.589907226107435
1	55	-17.4923040104532
1	56	-16.830306867755805
2	1	0.33902958936374716
2	2	0.7509854393141708
2	3	0.7143283547858914
2	4	0.4959991682599991
2	5	0.9852180614037422
2	6	0.6879540337601071
2	7	1.1722806233432834
2	8	1.0734671992030655
2	9	1.2324314076781278
2	10	0.2668585001533259
2	11	-5.214633884929473
2	12	-12.92821443515017
2	13	-13.227301401339162
2	14	-15.807962684801698
2	15	-9.413475821825301
2	16	-5.539474596686389
2	17	-4.626849715836464
2	18	-11.824805494652043
2	19	-4.556745495311482
2	20	-5.327091537683707
2	21	0.993474909897401
2	22	1.6176383508780532
2	23	0.9736888444446947
2	24	1.3283437769099748
2	25	1.302077159292665
2	26	1.5733454371728683
2	27	1.50430978999821
2	28	0.6918712421165552
2	29	0.696942810711235
2	30	1.7834358105517936
2	31	-3.852486593411303
2	32	-10.858591185163501
2	33	-4.252845758668656
2	34	-3.1968468597857544
2	35	-2.8205493284926497
2	36	2.9731393583208536
2	37	2.30002047875632
2	38	3.3387462172919946
2	39	4.928301539799687
2	40	7.210914066641234
2	41	2.9021035982489174
2	42	-1.1430851295720679
2	43	-4.023746843094413
2	44	-10.310756470914864
2	45	-3.1434542486057815
2	46	-14.363462298131113
2	47	-14.194523302070007
2	48	-12.787238157052293
2	49	-13.446170961220389
2	50	-12.663338950793367
2	51	-12.82844406899131
2	52	-13.138495462699025
2	53	-13.06461107993601
2	54	-15.42564296184317
2	55	-12.75206377021296
2	56	-11.792769330218267
3	1	0.8538771803175322
3	2	1.1911034334144688
3	3	1.8285859752382052
3	4	1.7441800925471185
3	5	1.7042937742847641
3	6	1.7721231586372
3	7	2.2599895712292266
3	8	2.70090082752165
3	9	3.0187637577272923
3	10	2.2699066810776145
3	11	0.7227309134972728
3	12	0.9117069021162969
3	13	-6.047065413138572
3	14	-5.153587070249092
3	15	-2.4363863429659105
3	16	1.9009383826646449
3	17	2.6726586420789715
3	18	1.569393128750118
3	19	0.675309568601989
3	20	0.8706469087273092
3	21	3.400947869582751
3	22	3.652643267298899
3	23	3.220001528810471
3	24	3.6795728821213842
3	25	2.5965707679455647
3	26	1.9379472070843704
3	27	1.88208756777599
3	28	1.9607994623722078
3	29	1.6844551017633478
3	30	1.709197855782861
3	31	2.09736591396333
3	32	0.6051256289957934
3	33	1.8266036021966343
3	34	1.9850607115023493
3	35	1.7314664040540606
3	36	3.5587834094516317
3	37	3.664425591834
3	38	4.4549704060825555
3	39	6.211094066840001
3	40	9.282988796238087
3	41	7.848121297363964
3	42	4.610602186062149
3	43	0.7442964214090289
3	44	1.5391943648766784
3	45	4.152219301050071
3	46	-2.733865444640454
3	47	-5.885870401381705
3	48	-2.6012007922538665
3	49	-9.230536742931303
3	50	-2.719779462101137
3	51	-5.067972092590129
3	52	-5.101327321106105
3	53	-5.271395740702971
3	54	-6.922004810417407
3	55	-4.8267933670664505
3	56	-2.1905077766292784
4	1	0.9288193791535377
4	2	1.3257602140889446
4	3	1.617631658089195
4	4	1.4587619310227637
4	5	1.6539868301725136
4	6	1.3137798595859351
4	7	2.390899241961904
4	8	2.4455392712751376
4	9	2.578677653924373
4	10	2.140432073726899
4	11	4.309990739695152
4	12	2.848962742027009
4	13	4.4474733734356136
4	14	3.8692144923754768
4	15	5.251088742739263
4	16	5.9353001780883865
4	17	5.953931064944321
4	18	4.944263574417025
4	19	3.7795908410248558
4	20	4.123058612466444
4	21	3.4697511861736743
4	22	3.573794507034208
4	23	2.6718870426428936
4	24	3.390806239372438
4	25	2.4747498319653403
4	26	2.5589310227584505
4	27	2.2244299101183316
4	28	2.38406343430875
4	29	1.8071530209214473
4	30	2.5867391138550957
4	31	2.2195854786607683
4	32	2.680147553575239
4	33	2.3063134004905024
4	34	2.256606593667698
4	35	1.5296008216575032
4	36	3.3557219409034325
4	37	4.1961165748524145
4	38	4.136944415490195
4	39	6.443016254514401
4	40	8.747550703277867
4	41	2.292193688339008
4	42	5.573131086644146
4	43	4.243220123872554
4	44	7.6249792648208725
4	45	6.47606527091374
4	46	5.011012176343364
4	47	4.5204917129450095
4	48	4.491440521626384
4	49	0.6069821919327651
4	50	5.229754142299726
4	51	2.7499343093870685
4	52	2.937180212976653
4	53	2.6750285597036303
4	54	-0.2971144333146398
4	55	3.584272566123378
4	56	3.575098537649602
5	1	1.2239969096251855
5	2	1.4137305373533877
5	3	1.7816486162237979
5	4	1.6535155081292814
5	5	1.0943683882011328
5	6	1.8474311579431202
5	7	2.3180322573302092
5	8	3.0499377874383597
5	9	2.9043268325147267
5	10	2.387119938061822
5	11	4.394516441867914
5	12	6.2077332772681295
5	13	6.300149579052999
5	14	5.914259537420527
5	15	6.761687576867509
5	16	6.905623442529301
5	17	6.424784271091639
5	18	6.21506378639371
5	19	4.995542086387864
5	20	4.851993429636558
5	21	3.1411873281980576
5	22	3.342474952185242
5	23	2.816826099346656
5	24	3.8629842997857935
5	25	3.279201342299203
5	26	2.7184434763885506
5	27	2.5781659991485384
5	28	2.7735705885217854
5	29	3.021779412018425
5	30	3.240952150421073
5	31	3.245552622274971
5	32	4.794349991307087
5	33	3.3889843066908227
5	34	3.3456368003449626
5	35	3.395790540788397
5	36	4.328694913876408
5	37	5.0575368186255965
5	38	5.419138374154738
5	39	7.113922454832366
5	40	6.333136288863454
5	41	2.868770264915586
5	42	9.219659968467148
5	43	12.652070150369639
5	44	11.621269672875986
5	45	9.149356208910557
5	46	10.121505022130325
5	47	10.296326371132608
5	48	10.316029816803919
5	49	10.640103548583532
5	50	9.914438826984409
5	51	8.900967695714574
5	52	9.486994733379408
5	53	7.731467351436542
5	54	8.67108906430062
5	55	8.525714007564819
5	56	7.896773153441863
6	1	1.1176620679962213
6	2	1.111882875211606
6	3	0.9307813462388808
6	4	1.5079649802258075
6	5	1.5636368648225485
6	6	1.4024839482900262
6	7	2.4848874609501195
6	8	2.8734671992030627
6	9	3.1454655957123165
6	10	2.1865166198114494
6	11	3.8221182518226655
6	12	6.1728539409182055
6	13	5.736801162763406
6	14	7.186695434856425
6	15	6.447207938858462
6	16	6.936166428954639
6	17	6.456269942283193
6	18	6.129468009621458
6	19	5.330006641440654
6	20	5.08295119735903
6	21	2.9092868757093697
6	22	3.605672538912245
6	23	2.399970895726746
6	24	4.090736939303142
6	25	3.2230173302328353
6	26	1.1464223602497867
6	27	2.4878567985452236
6	28	2.123495173740487
6	29	2.3266436654120923
6	30	2.6114272635432485
6	31	1.843026227101518
6	32	3.7010241994518864
6	33	3.03924825842536
6	34	2.837341174402276
6	35	2.261929303985987
6	36	4.290233375414868
6	37	3.9344221881580275
6	38	3.9419513454971202
6	39	5.425951112449258
6	40	3.259418340145505
6	41	2.932872829018148
6	42	7.930632819145881
6	43	12.140569396221824
6	44	11.066593956435565
6	45	6.880691050539518
6	46	9.82927274460393
6	47	9.83026302271632
6	48	9.939898595084458
6	49	10.873700833651405
6	50	8.754182416728
6	51	9.422837982290744
6	52	9.059581460377899
6	53	9.692440202115275
6	54	9.483117721917512
6	55	7.714816571667381
6	56	8.12603408858515
7	1	1.1445851449193043
7	2	1.35765210598084
7	3	1.7465505770081151
7	4	1.5948880571488857
7	5	2.196329172514858
7	6	2.040176255982331
7	7	2.394502845565505
7	8	2.99346719920306
7	9	2.7535425187892386
7	10	2.3657473890422125
7	11	4.7198105595149755
7	12	6.644007787072052
7	13	6.05603193199417
7	14	7.490926204087188
7	15	6.318746400396918
7	16	6.293858736646943
7	17	6.1520391730524295
7	18	5.794083394236846
7	19	4.407698949132964
7	20	4.611797351205183
7	21	2.982363798786288
7	22	3.0287494619891646
7	23	2.130355511111361
7	24	3.6761215546877537
7	25	2.6876327148482204
7	26	2.473345437172867
7	27	2.842087567775991
7	28	2.509649019894333
7	29	2.9647205884890155
7	30	1.9556580327740178
7	31	3.2941800732553617
7	32	3.4814088148364988
7	33	2.922709796886899
7	34	3.354264251325354
7	35	2.535006227062908
7	36	0.37869491387640863
7	37	0.5255760343118752
7	38	1.255412883958659
7	39	2.6794126509107983
7	40	-3.680197044469878
7	41	-10.167896401751081
7	42	3.2702482037612626
7	43	7.384030934683363
7	44	5.838132417974023
7	45	3.198767973616441
7	46	5.545426590757778
7	47	6.251032253485551
7	48	6.237206287392151
7	49	8.757162372112944
7	50	6.201105493651077
7	51	7.748222597675358
7	52	8.601504537300976
7	53	8.222055586730658
7	54	8.416579260379052
7	55	8.62904734089815
7	56	8.628341780892843
8	1	0.8782586143070574
8	2	1.3809174121032868
8	3	1.4957342504775042
8	4	1.6767247918427657
8	5	1.7208189684332211
8	6	1.608135439655804
8	7	2.2598089680144824
8	8	2.8734671992030627
8	9	3.1170119065443416
8	10	2.6190126951646633
8	11	4.0685860697190535
8	12	6.44502819523532
8	13	6.608072748320701
8	14	7.050313959189232
8	15	6.163848441213247
8	16	6.483654655014295
8	17	6.051835091419775
8	18	5.325307884032764
8	19	4.663209153214595
8	20	4.178123881817427
8	21	2.194608696745476
8	22	3.059361706887124
8	23	2.1709677560093184
8	24	2.981631758769389
8	25	2.633142918929856
8	26	2.4121209473769483
8	27	1.7392304249188442
8	28	1.7553633056086184
8	29	1.7216593639992155
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115	12	-5.970398992588965
115	13	-1.722781627327862
115	14	-6.690260236590774
115	15	-3.023456989433587
115	16	-7.472242958268307
115	17	-10.785418454066217
115	18	-1.7613403345767153
115	19	-1.3495891864602605
115	20	-4.477185699642273
115	21	-0.002381963925571995
115	22	0.3762070891078082
115	23	-0.5206614380411807
115	24	1.2315452835013119
115	25	-0.3203333868466913
115	26	1.8377522168338842
115	27	1.3058163813353119
115	28	1.5120219012502645
115	29	2.374720588489012
115	30	2.3022682022655374
115	31	0.6785868529163821
115	32	-4.634862371604179
115	33	0.49931996637842246
115	34	0.812569336071121
115	35	-0.04804462039471957
115	36	2.7015762698086085
115	37	3.3335421360067876
115	38	3.029141697517982
115	39	5.991955023792155
115	40	8.508277531801305
115	41	5.43939173384214
115	42	2.029231254608721
115	43	1.0709800872257382
115	44	-0.8320370735513976
115	45	1.5516493295486455
115	46	-2.0491496804286626
115	47	-7.328798254989026
115	48	-4.81059032277734
115	49	-6.145041017717563
115	50	-1.3510978961794322
115	51	-0.04652316503650766
115	52	-0.9079869881227545
115	53	-0.5013342437778157
115	54	-0.7220648074175564
115	55	-0.9629865574069321
115	56	2.326816357164031
116	1	0.44868350557504044
116	2	1.2176521059808394
116	3	1.2906489376638532
116	4	1.3717733030505244
116	5	2.2258373692361673
116	6	0.677389370736428
116	7	0.8469618619589454
116	8	1.586581953301426
116	9	1.4763294040351411
116	10	1.1442719792061524
116	11	-1.8701894404850279
116	12	-0.7125495899771295
116	13	-5.1087221663664835
116	14	-10.058418058207891
116	15	-5.386335566816193
116	16	-10.801223230566169
116	17	-11.019928040062329
116	18	-3.8916543106811865
116	19	-3.9483666246375293
116	20	-4.466071501253833
116	21	0.8856424873108821
116	22	1.0369461833006426
116	23	1.5501915766851297
116	24	0.8372690956713598
116	25	1.5215671410777318
116	26	1.4979356011072937
116	27	2.4558580595792705
116	28	1.8876818067795824
116	29	1.5550484573414707
116	30	2.0794285245772954
116	31	0.12565548309142827
116	32	-4.812689545819239
116	33	0.9486114362311611
116	34	0.6519691693581464
116	35	1.1153340959153653
116	36	3.443449012237064
116	37	3.5244284933282692
116	38	5.310330916745546
116	39	6.577117568943589
116	40	9.18013082438258
116	41	4.349644581855475
116	42	1.985330170974379
116	43	-10.470559229251062
116	44	-2.189080696780074
116	45	-0.34582219031798545
116	46	-5.033589802684848
116	47	-8.463557910448877
116	48	-6.529678958509489
116	49	-4.835788447559185
116	50	-3.2057797522505638
116	51	-1.4863675662590694
116	52	-1.5089872659777122
116	53	-1.9045017903185233
116	54	-4.4722731986373425
116	55	-2.083575609921521
116	56	0.49047292843382806
117	1	-0.12359667326251866
117	2	-0.17628728795855153
117	3	0.44564148609902077
117	4	-0.14238467012383893
117	5	0.7539049300906129
117	6	-0.10800556219948731
117	7	0.23692708798974849
117	8	0.1310429567788205
117	9	0.5041485793952987
117	10	-0.938192004897175
117	11	-8.264128834424422
117	12	-11.287810394746131
117	13	-7.690937764975523
117	14	-10.116043492882504
117	15	-4.970041478390957
117	16	-10.748565505777297
117	17	-10.240991129977878
117	18	-3.7813711512177015
117	19	-3.5668465054124923
117	20	-3.289717800309969
117	21	-0.826727110304617
117	22	0.40753734077703996
117	23	-0.859644488888641
117	24	-6.172666324100124
117	25	-0.09691273969723113
117	26	-0.23877577494834412
117	27	0.30632999201841216
117	28	-0.181866131620815
117	29	0.07169028545870759
117	30	-0.4337359066199227
117	31	-1.3373350782597875
117	32	-11.93737906395138
117	33	-2.751835657658553
117	34	-0.8130084759473704
117	35	-1.5195392274825466
117	36	0.7226343078158024
117	37	-0.041393662657821295
117	38	1.3023825809283558
117	39	4.021230832728982
117	40	1.575560531287696
117	41	3.1414975376428558
117	42	-0.461266947753888
117	43	-5.764150883498454
117	44	-1.4945948547532488
117	45	-0.7909289960805275
117	46	-4.0291188637876765
117	47	-7.514725322272026
117	48	-6.530672500486636
117	49	-6.940110355159781
117	50	-3.873439960894377
117	51	-4.416322856870096
117	52	-4.405162129365692
117	53	-4.904005019329947
117	54	-9.451905588105795
117	55	-5.580346598495787
117	56	-1.6059006433495817
118	1	-0.022806159428526485
118	2	0.26113036685040214
118	3	0.9930723161385515
118	4	0.45445327454019235
118	5	0.6702422159931132
118	6	-0.07243243966983925
118	7	0.9510245846959435
118	8	0.5473802426813208
118	9	0.5957164318327131
118	10	0.06444304121612987
118	11	-9.174537266571985
118	12	-11.432079169449686
118	13	-11.68962024191887
118	14	-2.0712477089562853
118	15	-17.656470990907426
118	16	-7.745271698135664
118	17	-11.82926517477366
118	18	-3.4967861709805454
118	19	-3.7862140943453007
118	20	-4.812115692273078
118	21	-0.09154924469196857
118	22	-0.05820705974996798
118	23	-0.12051405410603167
118	24	-2.554748010529636
118	25	-0.1206281547169965
118	26	1.2124758719554762
118	27	1.2299136547325134
118	28	0.10008380250302906
118	29	1.7225466754455354
118	30	0.7882667284261871
118	31	-2.098863405005506
118	32	-7.580330315598282
118	33	-0.4059858552870139
118	34	-0.5857357486746437
118	35	-0.3910807294588352
118	36	2.437390566050322
118	37	3.133836903877093
118	38	4.061934623089098
118	39	5.926369172649931
118	40	7.260672520747512
118	41	3.462973163466309
118	42	-0.46192570928221244
118	43	2.075770065118146
118	44	-1.3088241037651045
118	45	-0.4299276785574726
118	46	-5.857182104894399
118	47	-7.134619920427493
118	48	-9.025402408260023
118	49	-6.951968062669664
118	50	-6.63628581069675
118	51	-7.809603489281164
118	52	-7.673278071394677
118	53	-8.45011832631282
118	54	-11.79907291353399
118	55	-9.858343963449675
118	56	-7.942527784324547
119	1	0.38208514491930146
119	2	1.2801521059808394
119	3	0.9532172436747786
119	4	0.9598880571488877
119	5	1.933829172514855
119	6	0.3768429226489971
119	7	0.9111695122321706
119	8	0.2068005325363984
119	9	0.44354251878923634
119	10	-1.7734192776244484
119	11	-9.01602277381836
119	12	-8.918492212927948
119	13	-2.3481347346724952
119	14	-3.7315737959128086
119	15	-5.695420266269746
119	16	-8.818641263353054
119	17	-9.125460826947574
119	18	-1.5484166057631548
119	19	-2.195634384200371
119	20	-2.159035982128149
119	21	-0.18846953454704618
119	22	-0.4712505380108354
119	23	-0.8388111555553053
119	24	-7.585545111978913
119	25	-0.5590339518184422
119	26	1.4525121038395312
119	27	1.0487542344426544
119	28	0.9696490198943337
119	29	1.3538872551556764
119	30	0.4639913661073507
119	31	-1.7691532600779674
119	32	-11.787757851830168
119	33	-1.0681235364464357
119	34	-1.2940690820079759
119	35	-0.835827106270429
119	36	3.391194913876408
119	37	2.8847427009785385
119	38	3.8554128839586603
119	39	5.547745984244134
119	40	8.833136288863457
119	41	2.6604369315822503
119	42	-0.12225179623873572
119	43	-1.6543023986499676
119	44	-2.0343675820259755
119	45	-2.6753986930502265
119	46	-9.55374007590889
119	47	-10.00146774651445
119	48	-11.534460379274515
119	49	-8.921170961220389
119	50	-8.46056117301559
119	51	-11.440944068991309
119	52	-11.28016212936569
119	53	-12.464611079936008
119	54	-11.686754072954281
119	55	-13.328452659101849
119	56	-10.244158219107156
120	1	-0.04454528986330786
120	2	1.0219999320677928
120	3	0.7756810117907236
120	4	0.932714144105411
120	5	1.4745900420800737
120	6	0.49278495163450486
120	7	1.0814593673046389
120	8	0.721293286159586
120	9	0.7478903448761898
120	10	0.34705173686830193
120	11	-12.022363353528505
120	12	-6.1712096042322955
120	13	-8.472228937571046
120	14	-11.614725969825852
120	15	-8.482557947429164
120	16	-13.13657604596175
120	17	-12.524917348686703
120	18	-6.083742692719678
120	19	-5.916648876953996
120	20	-7.790376561838293
120	21	-2.982853592518058
120	22	-2.0799461901847494
120	23	-3.055296662801684
120	24	-9.641704532268768
120	25	-3.3162803286300395
120	26	-1.244045867174961
120	27	-0.4874776496153146
120	28	-0.6173075018447989
120	29	-1.8861489767283786
120	30	-2.2552115324433757
120	31	-3.8162547093533306
120	32	-10.64554770690263
120	33	-3.5581597683304906
120	34	-12.694431400848558
120	35	-3.8693415990240503
120	36	-1.584348564384463
120	37	-2.2139891830794305
120	38	-1.6989349421282967
120	39	-1.3345003925674632
120	40	-5.413240522730749
120	41	-2.9283311843597772
120	42	-5.940186578847431
120	43	-5.9459690653166355
120	44	-6.308824103765106
120	45	-10.125579852470516
120	46	-15.487616887503092
120	47	-14.134619920427493
120	48	-11.634098060433937
120	49	-14.34327241049575
120	50	-10.266720593305445
120	51	-11.287864358846381
120	52	-10.564582419220763
120	53	-11.515335717617168
120	54	-12.62515987005573
120	55	-11.510517876493154
120	56	-8.20339734954194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	497.0
5	70.0
6	119.0
7	74.0
8	165.0
9	207.0
10	131.0
11	129.0
12	133.0
13	178.0
14	221.0
15	296.0
16	399.0
17	525.0
18	643.0
19	817.0
20	963.0
21	1218.0
22	1539.0
23	1806.0
24	1898.0
25	2079.0
26	2231.0
27	2335.0
28	2551.0
29	2611.0
30	2942.0
31	3489.0
32	4150.0
33	4904.0
34	5831.0
35	6641.0
36	7027.0
37	5407.0
38	2578.0
39	726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.2714349178143	0.564193691692581	0.09625351695542722	0.06811787353768696
8	98.66725899600178	0.9121871760698949	0.19546868058640607	0.22508514734192211
9	95.91226173373421	2.3608169552274174	1.0767339563678373	0.6501873546705372
10	42.418184510587885	38.92047978676144	9.33659114467644	9.324744557974233
11	28.39308720956062	30.986124068890962	19.531446680587024	21.08934204096139
12	34.466163186731826	18.35184362505553	20.453131941359395	26.728861246853253
13	22.807641048422923	13.090478305938102	22.150155486450466	41.951725159188506
14	26.167130754191597	17.598791397594642	24.598613661946796	31.635464186266958
15	28.27560631906846	16.299043239635097	21.275680486538604	34.14966995475785
16	26.362491667283905	23.401229538552702	23.918228279386714	26.31805051477668
17	34.35574644549763	19.982227488151658	22.15936018957346	23.50266587677725
18	31.383553732470492	23.090820240193104	21.336018599416548	24.189607427919857
19	31.439107833118584	24.841160527835786	21.3681669406555	22.35156469839013
20	26.65985245755933	30.415074215625275	20.84407311942642	22.081000207388975
21	25.98154648183528	23.277202648065046	29.195361443106588	21.545889426993085
22	34.81319136963379	22.177138710776113	19.99585363325386	23.01381628633624
23	28.32815045165112	29.914112246409	20.695986968754628	21.061750333185252
24	31.9398785724863	24.18480675255442	23.414778616911004	20.460536058048277
25	28.497386308104666	28.909061292185577	21.63218765271217	20.961364746997585
26	25.8637826931921	22.472443514768496	29.97314818935719	21.690625602682214
27	31.83346416458077	18.408697068886354	25.666128530592296	24.091710235940578
28	31.056066449124884	23.053989913972114	26.465440522100263	19.42450311480273
29	27.656548597840096	22.06716738515325	29.468319926521787	20.80796409048487
30	21.961010873107575	23.606790507510446	32.034189553521166	22.398009065860812
31	20.763202422299404	29.655356665776118	31.075785911479205	18.505655000445277
32	21.655928312226912	25.219580833888767	31.64037621269348	21.484114641190846
33	20.754772959902688	27.436175104953197	33.28685229413597	18.522199641008143
34	18.761117040199217	28.151310328471478	36.01772797343769	17.06984465789162
35	18.2973994220938	29.584352078239608	37.63206638512262	14.486182114543972
36	19.170930663700407	29.824249165739715	34.47534297367446	16.529477196885427
37	19.878284102716343	27.53896393053288	38.01840581861363	14.564346148137153
38	22.29537366548043	29.18594306049822	34.1785290628707	14.340154211150653
39	17.74452402888816	24.583172348203405	31.751716349154457	25.920587273753977
40	12.568696662347529	17.76506858496046	27.389303427013985	42.27693132567803
41	14.430988397270298	19.969537234757418	29.295025908283183	36.3044484596891
42	18.849441937782	29.274519116599386	36.26959154595108	15.606447399667536
43	12.014260249554368	16.391859774212715	28.954248366013076	42.639631610219844
44	12.155789285767417	18.938097859236226	26.1205909043574	42.78552195063895
45	18.66168468187951	28.065180967631925	37.58136500452234	15.691769345966222
46	18.275059657027672	28.158117061168834	36.30704471683291	17.259778564970578
47	16.583956743944896	26.461743574550034	34.30264424857418	22.651655432930895
48	18.768054753122083	29.209072189384177	36.37912389079004	15.643749166703703
49	12.300585247021777	19.62211461335076	25.547369359199074	42.52993078042839
50	17.30760667903525	30.33617810760668	36.244897959183675	16.1113172541744
51	16.930139957804656	31.720797551481294	35.25391495557603	16.095147535138025
52	16.89332798263997	30.882418513399028	36.011652621096594	16.212600882864407
53	15.694335198349682	31.842804351375015	36.42124634540894	16.041614104866355
54	14.957594448727834	28.628195243461242	31.341557440246724	25.072652867564198
55	16.735147964102946	33.17659274642142	34.49529036564562	15.592968923830009
56	18.888081071857798	31.571138181224683	33.986675964805556	15.554104782111963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	13.5
13	67.0
14	109.0
15	138.5
16	168.0
17	191.0
18	214.0
19	249.5
20	285.0
21	349.0
22	489.5
23	566.0
24	700.5
25	835.0
26	956.5
27	1078.0
28	1308.5
29	1805.5
30	2072.0
31	2291.5
32	2511.0
33	2528.0
34	2545.0
35	2656.0
36	2767.0
37	2872.0
38	2881.0
39	2785.0
40	2530.0
41	2275.0
42	2031.5
43	1788.0
44	1724.5
45	1661.0
46	1602.0
47	1675.5
48	1808.0
49	1890.0
50	1972.0
51	1962.0
52	1952.0
53	2027.5
54	2187.5
55	2272.0
56	2509.0
57	2746.0
58	2976.0
59	3206.0
60	3399.5
61	3593.0
62	3597.0
63	3453.0
64	3305.0
65	3157.5
66	3010.0
67	2794.0
68	2578.0
69	2375.5
70	2173.0
71	1932.5
72	1486.5
73	1281.0
74	1063.0
75	845.0
76	710.0
77	575.0
78	459.5
79	258.0
80	172.0
81	128.5
82	85.0
83	62.5
84	40.0
85	28.5
86	17.0
87	9.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.016289056715533837
10	0.0
11	0.004442470013327411
12	0.0
13	0.0
14	0.020731526728861246
15	0.1702946838442174
16	0.03702058344439508
17	0.014808233377758034
18	0.0014808233377758032
19	0.01332741003998223
20	0.035539760106619286
21	0.01332741003998223
22	0.0014808233377758032
23	0.0
24	0.0
25	0.0014808233377758032
26	0.18214127054642382
27	0.019250703391085443
28	0.16289056715533837
29	0.039982230119946685
30	0.035539760106619286
31	0.23100844069302534
32	0.02221235006663705
33	0.1762179771953206
34	0.09773434029320303
35	0.06663705019991116
36	0.15548645046645934
37	0.23693173404412854
38	0.13327410039982232
39	0.3494743077150896
40	0.5730786317192359
41	0.8337035391677773
42	0.22804679401747371
43	0.3109729009329187
44	0.4605360580482748
45	0.1288316303864949
46	0.090330223604324
47	0.03702058344439508
48	0.039982230119946685
49	0.3080112542573671
50	0.2295276173552495
51	0.33170442766177993
52	0.36872501110617506
53	0.22064267732859472
54	0.1273508070487191
55	0.1702946838442174
56	0.19694950392418184
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	67530.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.21353472530727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.57952593610443	80.67821708870132
2	4.154929577464789	7.164223308159337
3	1.0048093438680865	2.598844957796535
4	0.4362761937478529	1.5045165111802161
5	0.228443833734112	0.9847475196209092
6	0.14256269323256612	0.7374500222123501
7	0.07729302645139127	0.46645935139937805
8	0.07557540364136035	0.5212498148970828
9	0.06011679835108211	0.46645935139937805
>10	0.21813809687392646	3.2652154597956464
>50	0.017176228100309172	1.061750333185251
>100	0.005152868430092752	0.5508662816525989
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA	146	0.2162002073152673	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	121	0.17917962387087222	No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA	105	0.15548645046645934	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	96	0.14215904042647715	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	94	0.13919739375092552	No Hit
GAGGAGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACCAAAAAAAAAAAAAAA	93	0.13771657041314972	No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	68	0.10069598696875463	No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAACCAAAAAAAAAAAAAAA	68	0.10069598696875463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.00444247001332741	0.0	0.0	0.0	0.0
6	0.00444247001332741	0.0	0.0	0.0	0.0
7	0.00444247001332741	0.0	0.0	0.0	0.0
8	0.00444247001332741	0.0	0.0	0.0	0.0
9	0.00444247001332741	0.0	0.0	0.0	0.0
10	0.019250703391085443	0.0	0.0	0.0	0.0
11	0.04146305345772249	0.0	0.0	0.0	0.0
12	0.045905523471049904	0.0	0.0	0.0	0.0
13	0.045905523471049904	0.0	0.0	0.0	0.0
14	0.05330964015992892	0.0	0.0	0.0	0.0
15	0.05330964015992892	0.0	0.0	0.0	0.0
16	0.05627128683548053	0.0	0.0	0.0	0.0
17	0.05775211017325633	0.0	0.0	0.0	0.0
18	0.06219458018658374	0.0	0.0	0.0	0.0
19	0.06219458018658374	0.0	0.0	0.0	0.0
20	0.06959869687546276	0.0	0.0	0.0	0.0
21	0.07107952021323856	0.0	0.0	0.0	0.0
22	0.07700281356434177	0.0	0.0	0.0	0.0
23	0.09033022360432401	0.0	0.0	0.0014808233377758032	0.0
24	0.09477269361765141	0.0	0.0	0.0014808233377758032	0.0
25	0.09773434029320302	0.0	0.0	0.0014808233377758032	0.0
26	0.09921516363097882	0.0	0.0	0.0014808233377758032	0.0
27	0.09921516363097882	0.0	0.0	0.0014808233377758032	0.0
28	0.10365763364430623	0.0	0.0	0.0014808233377758032	0.0
29	0.10958092699540944	0.0	0.0	0.0014808233377758032	0.0
30	0.10958092699540944	0.0	0.0	0.0014808233377758032	0.0
31	0.11550422034651266	0.0	0.0	0.0014808233377758032	0.0
32	0.11550422034651266	0.0	0.0	0.0014808233377758032	0.0
33	0.11550422034651266	0.0	0.0	0.0014808233377758032	0.0
34	0.11550422034651266	0.0	0.0	0.0014808233377758032	0.0
35	0.11550422034651266	0.0	0.0	0.0014808233377758032	0.0
36	0.11698504368428847	0.0	0.0	0.0014808233377758032	0.0
37	0.11698504368428847	0.0	0.0	0.0014808233377758032	0.0
38	0.11846586702206427	0.0	0.0	0.0014808233377758032	0.0
39	0.11846586702206427	0.0	0.0	0.0014808233377758032	0.0
40	0.11994669035984007	0.0	0.0	0.0014808233377758032	0.0
41	0.11994669035984007	0.0	0.0	0.0014808233377758032	0.0
42	0.11994669035984007	0.0	0.0	0.0014808233377758032	0.0
43	0.1258699837109433	0.0	0.0	0.0014808233377758032	0.0
44	0.1332741003998223	0.0	0.0	0.0014808233377758032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTC	50	5.456968E-12	50.64706	40
GCTTGTC	50	5.456968E-12	50.64706	38
CATGTCT	15	0.007980171	50.647057	39
TGCCTCA	20	3.914329E-4	50.60889	41
GATCCTC	15	0.0080518555	50.53273	37
GACCACC	20	3.94357E-4	50.53273	37
TCTTAGT	20	3.987756E-4	50.41892	36
ATGCTTG	75	0.0	50.41892	36
ATGCTGG	15	0.008124018	50.41892	36
AGACCAC	15	0.008124018	50.41892	36
ATGCATG	15	0.008124018	50.41892	36
TATGCTT	75	0.0	50.381096	35
TATGCAT	15	0.008148179	50.381092	35
AGAGTCC	15	0.008148179	50.381092	35
CGATTAA	40	2.5138434E-9	50.230362	49
TTAGAGC	15	0.008269787	50.192825	34
ACTCTTA	15	0.008269787	50.192825	34
ATATGCC	15	0.008269787	50.192825	34
CCCACAC	15	0.008269787	50.192825	43
CCATATC	15	0.008269787	50.192825	43
>>END_MODULE