SRR1526299_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23069
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG	213	0.9233170055052234	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG	163	0.7065759244007109	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACCCGCACGGATGAG	118	0.5115089514066496	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG	75	0.3251116216567688	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG	65	0.2817634054358663	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG	58	0.25141965408123457	No Hit
GCTCAAGGGAATTGCCGGGTACGCACAGTTCTGAGTCGGAGACCCGCACGGATGAG	55	0.23841518921496382	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	51	0.2210759027266028	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG	51	0.2210759027266028	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACCCGCACGGATGAG	49	0.21240625948242228	No Hit
GCTCAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	45	0.19506697299406128	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	45	0.19506697299406128	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	44	0.19073215137197105	No Hit
GCTCAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT	34	0.14738393515106854	No Hit
GCTCAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	32	0.13871429190688803	No Hit
GCTCAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT	31	0.1343794702847978	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACCCGCAGGGATGAG	30	0.1300446486627075	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	28	0.12137500541852701	No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCTACACGCAGGGATGAG	26	0.11270536217434654	No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG	25	0.10837054055225627	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	24	0.10403571893016603	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	24	0.10403571893016603	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	24	0.10403571893016603	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGACA	15	0.008008654	50.493393	38
TGGTCCA	15	0.008008654	50.493393	39
GACACGC	55	0.0	50.493393	41
GCGCTTG	20	3.903357E-4	50.493393	38
CCACACG	55	0.0	50.493393	40
GTCCACG	15	0.008008654	50.493393	41
CTTGCCT	30	9.3711606E-7	50.493393	41
AGACCCG	85	0.0	50.493393	40
AGACACG	15	0.008008654	50.493393	40
GAGACCC	85	0.0	50.493393	39
GAGACAC	15	0.008008654	50.493393	39
CGACACG	45	1.1277734E-10	50.49339	40
GACCCGC	90	0.0	50.49339	41
ACGCAGG	120	0.0	50.38242	44
TCGGCCA	55	0.0	50.38242	36
GAGCCCT	20	3.9461552E-4	50.38242	36
CCGCACG	70	0.0	50.38242	44
GCGTGGT	20	3.9461552E-4	50.38242	36
TGCCTGT	20	3.9461552E-4	50.38242	37
TCCACGC	20	3.9461552E-4	50.38242	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content