FastQCFastQC Report
Fri 17 Jun 2016
SRR1526298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174148
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG14080.8085077060890736No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG10690.6138456944667754No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10560.6063807795668053No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG8070.46339894802122333No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG6610.37956221145232794No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC6290.3611870363139399No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC5310.3049130624526265No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4690.26931116062199967No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG4380.25151020970668625No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3600.20672072030686542No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG2950.16939614580701473No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG2500.14355605576865654No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2240.12862622596871626No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC2180.1251808806302685No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC2050.11771596573029837No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2030.11656751728414913No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2000.11484484461492524No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG1770.10163768748420883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATCC203.9046397E-450.6801438
GCTCTAA150.00798099250.68013839
TATTCCC150.00813930450.42902836
TATGATT150.00816748250.3849735
TCAAGCC150.00816748250.3849735
TTATTCC251.9877385E-550.3849735
CAATACC252.0187752E-550.2532734
CATCACT150.00826193450.23867444
CACGATT950.050.18039747
CGTGCAC204.1004198E-450.18039348
CGATTCA1250.050.18039349
ACGATTC1250.050.18039348
TACGAAG204.1004198E-450.18039347
ATACCCC150.0082999450.18039346
CCGATTG204.1062938E-450.16584443
CCGATTC204.1062938E-450.16584443
CTTGAAC150.00830946350.16584443
TTCACAT150.00831899250.15130245
CTCGAAG204.1121745E-450.15130245
CATATTC150.00838593250.0497533
Produced by FastQC (version 0.11.5)