##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526298_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174148 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.84450008039139 39.0 36.0 39.0 33.0 40.0 2 36.54948664354457 39.0 37.0 40.0 31.0 40.0 3 35.65605691710499 39.0 36.0 40.0 26.0 40.0 4 35.861118129407174 39.0 37.0 40.0 27.0 40.0 5 35.769357098559844 39.0 36.0 40.0 27.0 40.0 6 34.722620989043804 38.0 35.0 39.0 21.0 40.0 7 35.64996439809817 38.0 35.0 39.0 28.0 40.0 8 35.60294691871282 38.0 35.0 39.0 28.0 40.0 9 35.690866389507775 38.0 36.0 39.0 28.0 40.0 10 35.32581482417254 38.0 35.0 39.0 27.0 40.0 11 34.37492247972988 38.0 33.0 39.0 25.0 40.0 12 32.120621540299055 37.0 29.0 39.0 15.0 40.0 13 31.660777040218665 36.0 28.0 39.0 13.0 40.0 14 30.994809013023406 36.0 26.0 39.0 11.0 40.0 15 31.652772354549004 36.0 28.0 39.0 14.0 40.0 16 32.21904357213405 37.0 30.0 39.0 15.0 40.0 17 32.26200128626226 36.0 30.0 39.0 16.0 40.0 18 32.57708385970554 36.0 30.0 39.0 18.0 40.0 19 33.433395732365575 37.0 31.0 39.0 23.0 40.0 20 33.52279095941383 37.0 31.0 39.0 23.0 40.0 21 34.907854238923214 38.0 35.0 39.0 26.0 40.0 22 34.628006063807796 38.0 34.0 39.0 25.0 40.0 23 34.87700691365965 38.0 35.0 39.0 26.0 40.0 24 34.47920734088247 38.0 34.0 39.0 24.0 40.0 25 34.712457220295384 38.0 35.0 39.0 26.0 40.0 26 35.03921377219377 38.0 35.0 39.0 27.0 40.0 27 35.01362059857133 38.0 35.0 39.0 27.0 40.0 28 34.77362358453729 38.0 35.0 39.0 27.0 40.0 29 34.69127983094839 38.0 35.0 39.0 26.0 40.0 30 34.42424259824976 38.0 35.0 39.0 25.0 40.0 31 34.12224659485036 38.0 34.0 39.0 23.0 40.0 32 33.0533396880814 38.0 33.0 39.0 15.0 40.0 33 34.317310563428805 38.0 34.0 39.0 25.0 40.0 34 33.97701380435032 38.0 34.0 39.0 23.0 40.0 35 34.090853756574866 38.0 34.0 39.0 24.0 40.0 36 32.1574407974826 36.0 31.0 38.0 18.0 39.0 37 31.950834922020352 35.0 31.0 38.0 17.0 39.0 38 31.216890231297516 35.0 30.0 38.0 10.0 39.0 39 29.462118428003766 34.0 27.0 38.0 4.0 39.0 40 25.40196269839447 31.0 12.0 38.0 4.0 39.0 41 22.1232629717252 26.0 9.0 33.0 4.0 37.0 42 25.859091117899716 30.0 21.0 34.0 4.0 37.0 43 21.715414475044216 26.0 8.0 33.0 4.0 37.0 44 23.076681902749385 28.0 10.0 33.0 4.0 37.0 45 25.57501091026024 30.0 20.0 35.0 4.0 38.0 46 25.333595562395203 30.0 15.0 36.0 4.0 38.0 47 24.603291453246662 30.0 10.0 36.0 4.0 38.0 48 24.615465006775846 30.0 11.0 35.0 4.0 38.0 49 22.113610262535314 27.0 5.0 35.0 4.0 38.0 50 24.496962353859935 30.0 7.0 35.0 4.0 38.0 51 25.96961205411489 32.0 4.0 38.0 4.0 40.0 52 25.942635000114844 32.0 4.0 38.0 4.0 40.0 53 25.546035555963893 32.0 4.0 38.0 4.0 40.0 54 24.568964329191264 31.0 4.0 38.0 4.0 39.0 55 24.815289294163584 31.0 4.0 37.0 4.0 39.0 56 24.267060201667547 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.9862383021837076 1 2 2.616221346389189 1 3 3.6764597771223606 1 4 3.5487519326702994 1 5 3.4168061492947714 1 6 5.000406102191015 1 7 3.6552848285463284 1 8 3.56642930484513 1 9 3.51775259667712 1 10 3.9045487858294834 1 11 -2.0676625988795756 1 12 -5.482620667686376 1 13 -19.837899926687136 1 14 -16.115879041850086 1 15 -9.410791000384823 1 16 -20.242922573345474 1 17 -20.419811112783858 1 18 -19.762214005474814 1 19 -5.4172446537832215 1 20 -9.587757956813658 1 21 -7.662298992067004 1 22 -2.4821243753630107 1 23 -2.491879911047679 1 24 -2.9267373960637464 1 25 -2.898135665512598 1 26 3.0751750384464316 1 27 3.1734174023143567 1 28 3.3172226096760227 1 29 2.612280152137707 1 30 2.9595111998509864 1 31 1.9162117709457291 1 32 -11.012990773310204 1 33 1.3306625979871214 1 34 1.4200673225573084 1 35 1.3229560955244821 1 36 4.22267735650993 1 37 4.332606423668359 1 38 4.719392206085541 1 39 6.7796516273849505 1 40 9.642182731940501 1 41 -1.4708258399553884 1 42 -7.175527755685174 1 43 -13.63768497671953 1 44 -16.09925771779946 1 45 -15.06021347386926 1 46 -18.029278258831024 1 47 -17.874966154791675 1 48 -16.115898205458336 1 49 -14.719865786815951 1 50 -15.95560542880579 1 51 -17.245168950074046 1 52 -17.353163011255123 1 53 -17.410297121450366 1 54 -18.028852819387467 1 55 -16.853892757840917 1 56 -16.12504226668942 2 1 1.979876731265172 2 2 2.5920084285053875 2 3 3.6758755512216794 2 4 3.3985409622061624 2 5 3.561564344685877 2 6 4.98054242156784 2 7 3.595304302743017 2 8 3.720275458691283 2 9 3.5359285135872156 2 10 3.7388233720028055 2 11 -1.2685713947250825 2 12 -9.177914403486444 2 13 -11.575387755314207 2 14 -13.473620035034116 2 15 -7.497516089641557 2 16 -1.7140683052506986 2 17 -1.3387984545560094 2 18 -9.216547014238202 2 19 -1.4463261208393696 2 20 -2.7620520171836667 2 21 3.128223565544161 2 22 3.676330671699631 2 23 3.0857896767484903 2 24 2.9703090174383675 2 25 2.999754629846052 2 26 3.3597579660673347 2 27 3.151411560055344 2 28 3.2142041091891684 2 29 3.5772265980968143 2 30 3.365028888912981 2 31 -0.32520335401370204 2 32 -8.54606445068768 2 33 -0.27933415631343195 2 34 -0.061269905615368714 2 35 -0.1744798019114171 2 36 4.589051910226257 2 37 4.890217588874464 2 38 5.350745662755454 2 39 7.314932380387219 2 40 10.143773124670133 2 41 6.760397788736594 2 42 2.4501781839448142 2 43 -2.3242802379983374 2 44 -9.70036125561186 2 45 1.099474938983711 2 46 -10.639794325043294 2 47 -11.915991795817314 2 48 -7.8161254044197115 2 49 -11.440800548257043 2 50 -7.871931297030391 2 51 -6.765000173702738 2 52 -6.733558986587809 2 53 -7.142332175004409 2 54 -14.248391930065818 2 55 -7.973074841579965 2 56 -6.870774171914997 3 1 2.4256929789623243 3 2 3.2534008335686764 3 3 4.211057104677437 3 4 3.992883388069359 3 5 3.973595603971553 3 6 5.411457023101093 3 7 3.928325637502624 3 8 4.142810669958891 3 9 3.9583448682553097 3 10 4.008954708545268 3 11 2.9901199941142877 3 12 3.387531011620233 3 13 -4.086292848319495 3 14 -5.103708583200753 3 15 -1.0353124882865927 3 16 3.7438416014468707 3 17 3.358592643084684 3 18 3.328811635550828 3 19 1.944474378358347 3 20 1.8178939030635561 3 21 3.910344561562468 3 22 4.346622464651432 3 23 4.150120811234736 3 24 4.235938660274286 3 25 3.6603150387127528 3 26 3.4600432219061616 3 27 3.358430042256572 3 28 3.2731272683975803 3 29 3.509716049573605 3 30 3.436253706170959 3 31 3.666952110418464 3 32 2.963860075371631 3 33 3.53223356223414 3 34 4.200764325085295 3 35 3.796177475083887 3 36 5.536220151742864 3 37 5.936705376358859 3 38 6.540916221975753 3 39 8.987957875127819 3 40 11.214537372605001 3 41 11.13105209431691 3 42 8.611110019684997 3 43 3.556031166292385 3 44 4.703468898307438 3 45 8.45657959221958 3 46 2.3268069704936423 3 47 -3.214113861545016 3 48 2.4457305413816783 3 49 -9.215730720004828 3 50 1.6854021551595402 3 51 0.4811221297381607 3 52 0.9899211346459253 3 53 -0.1678269155998784 3 54 -2.73582284466735 3 55 -0.272563758202061 3 56 3.440540976358612 4 1 2.6455463457383246 4 2 3.4451816554864862 4 3 4.549712323662575 4 4 4.22021311988491 4 5 4.21457573131093 4 6 5.695558894604083 4 7 4.2665949795825 4 8 4.370960390198135 4 9 4.346344096501497 4 10 4.181875796718138 4 11 4.868761707407742 4 12 5.164060048857351 4 13 6.811063894879428 4 14 4.762536834510993 4 15 7.882447496278331 4 16 7.272637665502433 4 17 6.914207152056328 4 18 6.202099306783701 4 19 5.249703010424721 4 20 5.287988442905345 4 21 4.1236862555620775 4 22 4.5088837033163 4 23 4.085341725763001 4 24 4.20545438475817 4 25 3.573782142706577 4 26 3.376404402689495 4 27 3.5347275533505282 4 28 3.1698569616254417 4 29 3.78368865231333 4 30 3.4769154850634933 4 31 4.008115529287842 4 32 4.8082069787094674 4 33 3.7473117023074565 4 34 3.935522187327237 4 35 3.562963120463017 4 36 5.065353858090543 4 37 5.589367927005206 4 38 6.25430615058853 4 39 8.216831114564435 4 40 9.911431063517597 4 41 6.664333977396211 4 42 9.126930972800952 4 43 8.209417236136105 4 44 11.446667817852099 4 45 9.562020456583848 4 46 9.289039268390614 4 47 9.486976240712366 4 48 9.00245444645596 4 49 4.893183036506844 4 50 9.476112168665232 4 51 7.977890410656549 4 52 7.938020690887484 4 53 6.900714469698212 4 54 1.9337218205844344 4 55 7.338713494354373 4 56 6.835196581220661 5 1 2.7264026423480416 5 2 3.6925900227578623 5 3 4.6622235628861475 5 4 4.5814417653601325 5 5 4.68539341788204 5 6 5.919206220991136 5 7 4.287222260483759 5 8 4.307148045563871 5 9 4.346185425109951 5 10 4.486114967395665 5 11 5.408032876815902 5 12 7.740010754945047 5 13 8.286919398132188 5 14 8.329403603432556 5 15 8.565726176132358 5 16 7.696924966502063 5 17 7.436302143329399 5 18 6.959120515859709 5 19 5.791883155344589 5 20 6.084452715397013 5 21 4.2026252728572615 5 22 4.522053428814793 5 23 4.217633998466233 5 24 4.680212410886064 5 25 4.0076558402789075 5 26 3.4236355869069826 5 27 3.3072853361822894 5 28 3.3440120364654504 5 29 3.714349254206809 5 30 4.007763848103636 5 31 4.3602470149809704 5 32 5.3109969506775165 5 33 3.9651146324391533 5 34 4.108712011201995 5 35 4.008274380842771 5 36 4.9567432905758615 5 37 5.109757200819139 5 38 6.040073326766663 5 39 7.86451451224783 5 40 6.8258675156252835 5 41 3.422095652966103 5 42 9.15768677752936 5 43 13.110221171186616 5 44 12.192198573656828 5 45 10.068684655028871 5 46 11.12347889392613 5 47 11.198551318725798 5 48 10.499743810183677 5 49 11.155374288424124 5 50 11.407804134603772 5 51 10.345307240402143 5 52 10.572388914296805 5 53 10.4570031458499 5 54 10.665395274860629 5 55 10.718110014161855 5 56 9.767285225638073 6 1 2.822340866286268 6 2 3.2154008335686797 6 3 4.588972597635184 6 4 4.30741859933697 6 5 4.123370251858873 6 6 5.683175332960246 6 7 4.058156623418121 6 8 4.08427545869128 6 9 4.31631669924122 6 10 4.2015744268551245 6 11 5.19254252932555 6 12 8.19184087077516 6 13 7.81922827844107 6 14 9.049249163278116 6 15 8.377926948333126 6 16 7.561897939475042 6 17 7.545522220549472 6 18 6.810811635550827 6 19 5.785319448780886 6 20 5.508344607288905 6 21 4.454316392548378 6 22 4.6211576759190365 6 23 4.490684191516422 6 24 4.349262603936253 6 25 4.033864334487397 6 26 3.4812263204977114 6 27 3.424289197186148 6 28 3.0827610712144846 6 29 3.5617160495736044 6 30 3.575408635748424 6 31 3.6524168991508574 6 32 5.0555220472026186 6 33 3.807585674910193 6 34 3.9144262969162824 6 35 3.282853531421921 6 36 3.6196004334330105 6 37 3.523888474950411 6 38 4.225648616341953 6 39 6.334831114564437 6 40 1.605157090914858 6 41 1.531840826711278 6 42 6.556574808417388 6 43 11.818622715588162 6 44 10.07222946168772 6 45 7.239171141515357 6 46 10.245285843733075 6 47 10.854674870849351 6 48 10.442153076592948 6 49 12.123621392671229 6 50 10.257317648117287 6 51 10.032164383259286 6 52 10.335554937462827 6 53 10.430933647780403 6 54 11.099557437022789 6 55 10.192055960107798 6 56 10.3634705538234 7 1 2.6250396614669924 7 2 3.511432962082729 7 3 4.435928420928356 7 4 4.282888478855035 7 5 4.173988725754455 7 6 5.549247622116873 7 7 4.051763049120929 7 8 4.190154976763573 7 9 4.186581759482188 7 10 4.2507953103892575 7 11 5.205120842578566 7 12 7.325720388847451 7 13 7.689316631854727 7 14 8.792863621109444 7 15 8.064834578855212 7 16 7.444998341081462 7 17 6.907096517738232 7 18 6.580819667679343 7 19 5.438733103399354 7 20 5.614898824156377 7 21 3.9528384809017965 7 22 4.2310050654772695 7 23 3.8002424244481503 7 24 4.013824852932238 7 25 3.8874065031620972 7 26 3.574897003228635 7 27 3.393188795579725 7 28 3.418921713784762 7 29 3.7988726760796254 7 30 3.389786145788584 7 31 3.6781518389098906 7 32 4.897481886560048 7 33 3.465417000211403 7 34 4.008884128241583 7 35 3.267287266361677 7 36 1.890765092067543 7 37 2.134892491014668 7 38 2.8316566484704673 7 39 4.764019869584516 7 40 -1.6229392946273116 7 41 -9.508576843971452 7 42 4.562068784321003 7 43 9.82731347864037 7 44 7.835249542009002 7 45 5.069227366414953 7 46 7.137542871845529 7 47 7.790835513419633 7 48 7.725992434022665 7 49 9.833918581426246 7 50 7.539791543699618 7 51 8.945995708560496 7 52 8.97306497762347 7 53 10.111544089547468 7 54 10.739204023368174 7 55 9.63995957456563 7 56 9.107518746594483 8 1 2.496559353681228 8 2 3.200879825165316 8 3 4.323023017803251 8 4 4.331384985891589 8 5 4.2798744535395485 8 6 5.287477853968646 8 7 4.267803682241649 8 8 4.413552769615656 8 9 4.267375522770635 8 10 4.281708880636636 8 11 5.291634966300343 8 12 8.070126585060876 8 13 8.179833320457877 8 14 8.834627314538626 8 15 8.384145435728087 8 16 7.762334914264958 8 17 7.673186086095697 8 18 6.79729903050881 8 19 5.778596759705255 8 20 5.7200420862805075 8 21 4.200467653052584 8 22 4.664115659112319 8 23 4.036734611684494 8 24 4.722002099734574 8 25 3.955645847092441 8 26 3.585797749069144 8 27 3.5002219702953923 8 28 3.67287871827331 8 29 3.425682436128227 8 30 3.765996871042539 8 31 3.97130765545338 8 32 4.966345576614383 8 33 3.5538041623051555 8 34 4.02047671708435 8 35 3.4193577331025935 8 36 1.238255895617879 8 37 1.463720407723521 8 38 2.472505759199098 8 39 3.836579013724098 8 40 -1.4139689595053113 8 41 -9.387352450599646 8 42 5.121347917661087 8 43 9.637815992899085 8 44 8.130649629754945 8 45 5.714835007061573 8 46 8.745554751296105 8 47 9.158237896059436 8 48 9.06447240432404 8 49 10.789033157377112 8 50 9.089384875008044 8 51 10.273878668973573 8 52 10.280428887042657 8 53 10.841286588956873 8 54 11.302347352989173 8 55 10.844728229015363 8 56 10.04925206642844 9 1 2.559211834028204 9 2 3.382433091633196 9 3 4.584069371828733 9 4 4.375096018691806 9 5 4.37824121960081 9 6 5.7126269458634695 9 7 4.35580178470844 9 8 4.232372232884835 9 9 4.2332199250476705 9 10 4.21976797524222 9 11 5.447639303519104 9 12 8.170356999807417 9 13 8.191421826828165 9 14 9.046507227794248 9 15 8.418572109623447 9 16 7.561026971733103 9 17 7.4812641560333475 9 18 6.925585829099212 9 19 5.581287190716363 9 20 5.895699445998588 9 21 4.286026069967733 9 22 4.8457705791448475 9 23 4.281651933451904 9 24 4.240198087807222 9 25 4.3123482054551445 9 26 4.133032772110617 9 27 3.8981279068635644 9 28 3.4997288131499644 9 29 4.126651533444573 9 30 3.4808925067161667 9 31 4.409739479796016 9 32 5.230102692363904 9 33 4.1366179329747155 9 34 4.096039200142087 9 35 4.04595030561547 9 36 3.8294391431104238 9 37 3.5492110555955705 9 38 4.164326035696792 9 39 5.567637566177339 9 40 0.3366409618825976 9 41 -1.3598043345790458 9 42 7.611961905191581 9 43 12.412687231717193 9 44 10.479423010074814 9 45 8.13581630280568 9 46 10.726511650184694 9 47 11.331320032139672 9 48 11.153249850786498 9 49 13.739460102348644 9 50 11.288253131988256 9 51 10.863164383259289 9 52 10.78103880843057 9 53 10.958933647780402 9 54 10.863847759603438 9 55 10.30115273430135 9 56 10.159986682855656 10 1 2.7829768495498683 10 2 3.290011712229763 10 3 4.604922388430161 10 4 4.294079687203073 10 5 4.176274017549247 10 6 5.516530981495812 10 7 3.997436958146153 10 8 4.27257671392141 10 9 4.210274858237035 10 10 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115 26 2.372504098275492 115 27 2.4946780860750337 115 28 2.5384832934367054 115 29 2.509716049573605 115 30 2.656519746859537 115 31 2.0430280102619705 115 32 -4.182755730575163 115 33 1.8339745637990816 115 34 2.220815185805165 115 35 1.9921868647552543 115 36 3.9537671000996717 115 37 4.154721808283746 115 38 4.774093060786395 115 39 7.4529422256755495 115 40 9.602545979803743 115 41 8.970840826711278 115 42 3.3899636973062783 115 43 1.2587893822548288 115 44 1.0732850172432755 115 45 2.770448919293134 115 46 -2.8294919340447002 115 47 -4.999325129150648 115 48 -5.053291367851497 115 49 -7.538989718439883 115 50 -1.3634045741049334 115 51 0.8145532721481779 115 52 1.123332715240604 115 53 0.8686558700026232 115 54 0.7095018814672329 115 55 1.1710004045522417 115 56 3.2551927760456216 116 1 1.7703408662862685 116 2 2.2634008335686744 116 3 3.3169725976351785 116 4 3.062085266003635 116 5 2.839370251858874 116 6 3.7845086662935756 116 7 3.1168232900847883 116 8 2.8669421253579515 116 9 3.1603166992412213 116 10 3.1589077601884554 116 11 0.693875862658885 116 12 -1.1174924625581717 116 13 -5.459438388225596 116 14 -7.884084170055214 116 15 -3.2554063850002066 116 16 -8.336768727191629 116 17 -8.639811112783857 116 18 -3.127855031115839 116 19 -3.241347217885785 116 20 -3.6236553927110933 116 21 2.155649725881716 116 22 2.5358243425857054 116 23 1.9640175248497584 116 24 2.339929270602923 116 25 1.975197667820737 116 26 2.2438929871643793 116 27 2.4416225305194814 116 28 2.578761071214487 116 29 2.8163827162402697 116 30 2.2620753024150915 116 31 0.8444168991508576 116 32 -6.405811286130717 116 33 1.4942523415768605 116 34 1.4677596302496099 116 35 1.3855201980885852 116 36 3.739600433433008 116 37 3.763888474950413 116 38 4.382981949675287 116 39 6.366831114564434 116 40 8.687823757581526 116 41 6.76917416004461 116 42 2.7419081417507236 116 43 -9.052043951078506 116 44 -0.39310387164561433 116 45 0.915171141515355 116 46 -5.600047489600254 116 47 -8.722658462483981 116 48 -8.187180256740387 116 49 -6.307045273995438 116 50 -4.712015685216045 116 51 -1.6451689500740443 116 52 -1.4377783958705095 116 53 -3.6210663522195965 116 54 -5.2791092296438755 116 55 -3.369277373225536 116 56 -0.8778627795099325 117 1 1.1094317753771747 117 2 1.5374917426595829 117 3 2.5946998703624544 117 4 2.449509508427873 117 5 2.417097524586147 117 6 3.0311753329602453 117 7 2.161520259781753 117 8 1.902093640509463 117 9 2.245316699241222 117 10 2.3887562450369444 117 11 -9.109912016128995 117 12 -10.746431856497566 117 13 -10.219135357922568 117 14 -10.86075083672188 117 15 -9.54843668803051 117 16 -12.523283878706778 117 17 -10.872841415814161 117 18 -2.296188364449172 117 19 -3.2442260057645704 117 20 -3.5463826654383652 117 21 1.0382254834574738 117 22 1.416884948646313 117 23 1.0697751006073304 117 24 -1.7858283051546557 117 25 1.2177734253964942 117 26 2.38449904777044 117 27 2.475107379004328 117 28 2.0946701621235775 117 29 2.3051705950281516 117 30 2.7613177266575164 117 31 1.6421441718781296 117 32 -10.456114316433748 117 33 1.9848584021829225 117 34 1.439880842370826 117 35 1.9883989859673719 117 36 2.8496004334330074 117 37 3.179343020404957 117 38 3.2728304345237724 117 39 5.694103841837162 117 40 4.4352480000057675 117 41 4.970840826711278 117 42 2.313574808417389 117 43 -6.549922738957294 117 44 -1.3288614474031917 117 45 1.373352959697172 117 46 -3.0630777926305583 117 47 -7.828870583696103 117 48 -5.074756014316144 117 49 -5.3426513346014985 117 50 -1.1468641700645286 117 51 -0.7959265258316215 117 52 -0.5408086989008112 117 53 -0.9956118067650515 117 54 -4.83153347206812 117 55 -2.3586713126194745 117 56 1.7886523720052168 118 1 1.5412499571953617 118 2 2.1397644699323095 118 3 3.2121241127866966 118 4 2.968448902367271 118 5 3.1254308579194827 118 6 3.9251147268996363 118 7 2.854702077963573 118 8 2.8111845496003767 118 9 2.5294076083321286 118 10 2.854665335946031 118 11 -5.8636998949168735 118 12 -7.7123409474066555 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0.32504599237051224 119 34 -0.11351021102022685 119 35 0.26202813459651964 119 36 3.488489322321893 119 37 3.3918249828869165 119 38 4.305839092532427 119 39 6.609688257421581 119 40 8.27417296393073 119 41 7.191078921949373 119 42 1.9504795703221518 119 43 -0.19458363361818698 119 44 0.8024516839099398 119 45 0.8646949510391657 119 46 -5.069571299124064 119 47 -7.503293383118903 119 48 -7.686227875788006 119 49 -6.161013527963693 119 50 -4.475507748708107 119 51 -6.051518156423253 119 52 -6.825079983172095 119 53 -9.691860003013248 119 54 -10.989902880437528 119 55 -10.939118643066804 119 56 -7.706116747763902 120 1 1.6060785712043 120 2 2.2165155876670397 120 3 3.2543496468155055 120 4 3.1449267960582787 120 5 3.0193702518588736 120 6 4.129535988697953 120 7 2.7976976070246806 120 8 2.8350295570519393 120 9 2.776054404159254 120 10 3.1504924596420096 120 11 -8.152572224772808 120 12 -0.9966181456182817 120 13 -3.9229356559851603 120 14 -4.397636082623521 120 15 -7.350597641830806 120 16 -9.13338074904955 120 17 -9.06844499256528 120 18 -2.535942462809828 120 19 -2.10320514138305 120 20 -3.2859504746783053 120 21 0.6583819663188706 120 22 1.0314527578862496 120 23 0.5418317325000288 120 24 -4.175917723932599 120 25 -0.05551271469292374 120 26 2.083455828694433 120 27 1.8486170660386065 120 28 1.6984332023620254 120 29 2.501519328262127 120 30 2.1232774882074423 120 31 -0.30476342871799744 120 32 -9.690838608535088 120 33 -0.7792995709914408 120 34 -6.580983539149294 120 35 -0.4722940095616863 120 36 0.8209119088428452 120 37 1.236347491343853 120 38 1.493583042571462 120 39 2.8274868522693524 120 40 -1.000482253347439 120 41 0.6942014824489817 120 42 -3.590113716172773 120 43 -3.427344497526594 120 44 -4.493541030115562 120 45 -10.182533776517431 120 46 -12.615238746430856 120 47 -12.880472670134255 120 48 -12.151879710292299 120 49 -14.789558935197626 120 50 -10.04589546663681 120 51 -10.815114305265302 120 52 -10.59296965270111 120 53 -11.59024668008845 120 54 -12.393754038387046 120 55 -11.967091580875808 120 56 -8.232398298635616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5075.0 5 724.0 6 401.0 7 214.0 8 215.0 9 155.0 10 174.0 11 220.0 12 263.0 13 343.0 14 495.0 15 709.0 16 1000.0 17 1232.0 18 1449.0 19 1715.0 20 2035.0 21 2558.0 22 3018.0 23 3549.0 24 4084.0 25 4404.0 26 4962.0 27 5287.0 28 5537.0 29 5795.0 30 6657.0 31 7723.0 32 9429.0 33 11197.0 34 13531.0 35 16147.0 36 19142.0 37 19722.0 38 11702.0 39 3285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.59517192273239 0.35774169097549213 0.03100810804602981 0.016078278246089532 8 99.59115235317087 0.33419849782943245 0.03904724716907458 0.03560190183062682 9 99.04889353465852 0.5697449357601099 0.1602407631825309 0.22112076639883294 10 52.839538783104025 25.436984633759792 13.531019592530491 8.192456990605692 11 36.05250766598142 30.674836057101512 18.049797296521312 15.22285898039576 12 34.471254335392885 12.297585961366195 18.315455819188276 34.91570388405265 13 25.56963042929003 12.783953878310403 21.835450306635735 39.810965385763836 14 21.542172826742483 14.55673300594469 33.264983774159276 30.636110393153558 15 30.22244464769531 17.32215070092786 25.224199124487317 27.23120552688951 16 36.89102324753135 17.434212415916544 21.276057972346525 24.39870636420558 17 28.493561393633765 20.565632430818006 28.59177742294924 22.349028752598993 18 28.672581617710396 22.66345077095357 28.69957217101674 19.964395440319294 19 34.622872862812216 26.451449870490872 17.522498980582245 21.40317828611467 20 26.668542939852042 33.79876395717502 17.881266369526262 21.651426733446673 21 23.74506842470985 25.03316430544469 30.206795954816144 21.014971315029314 22 23.00598348512168 21.880491082195398 25.46369138538927 29.64983404729365 23 23.971564416473345 23.637940142866988 23.195787491099523 29.194707949560144 24 24.35973999127179 24.997128878884627 27.779245239681188 22.86388589016239 25 32.7342157397571 24.262539837491744 19.37925292141606 23.623991501335095 26 21.75806349553322 23.07710007535708 24.13957742508873 31.02525900402096 27 21.406497713852445 20.43172575419893 24.79838246444409 33.36339406750454 28 19.377958779846647 31.390820664147213 23.988334570052977 25.242885985953166 29 31.15630707729774 25.288042916384274 23.313383800673154 20.24226620564484 30 24.276194852941178 21.39705882352941 34.97874540441177 19.34800091911765 31 23.308681764553537 27.968116025438118 29.61238525510057 19.110816954907772 32 34.96054490529629 19.42316306957191 24.982483546019456 20.633808479112346 33 26.327902052839914 21.4817499769861 26.74733038755408 25.443017582619902 34 20.822531972984624 28.125305359965513 33.367437850265844 17.68472481678402 35 20.50820971837621 26.61408004044536 37.78883386379566 15.088876377382771 36 22.335591777575832 33.26412294525669 26.741858672770984 17.65842660439649 37 22.258612254295336 25.52680806976142 35.005036406020665 17.209543269922584 38 30.818734975097485 26.195953484627154 26.492713281726267 16.492598258549098 39 20.990870527480634 20.38569715971966 23.977545186278128 34.64588712652158 40 14.37525635026545 14.918285644962825 24.269943328538503 46.43651467623322 41 16.405123125314983 17.209738804024816 27.130435790046864 39.254702280613344 42 24.625512887650988 23.301356382825674 30.327269797607197 21.74586093191614 43 11.78141874953175 13.157636915842069 28.33637814879062 46.724566185835556 44 13.610447468347125 14.605334533661116 21.3871865009291 50.39703149706266 45 20.32723175274801 22.402612335004367 36.064480522467 21.205675389780616 46 18.56550994960552 24.503094347428846 29.229372453699714 27.702023249265917 47 16.03641899072292 26.315305741448142 30.347818594364824 27.30045667346411 48 19.514324943843462 30.210206414771324 31.632647199067026 18.642821442318187 49 15.239598757785448 18.20858372560656 20.852610897609487 45.6992066189985 50 24.86951217406817 22.16480310293432 31.550143579768775 21.41554114322873 51 19.96623801622419 30.28035121681416 31.906803097345133 17.846607669616517 52 18.64390170794833 24.85257921525452 35.6801530985745 20.82336597822265 53 20.52618558598986 22.12959606942934 37.486551948313455 19.85766639626734 54 16.533101846528847 25.103479200680663 29.152390369535723 29.211028583254766 55 20.172673622694905 28.904713150114464 29.809495323662382 21.11311790352825 56 25.23461819520924 22.838088990925986 27.987893643587487 23.939399170277284 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 4.5 9 6.0 10 37.0 11 68.0 12 223.5 13 645.0 14 911.0 15 1105.5 16 1300.0 17 1385.5 18 1471.0 19 1555.5 20 1640.0 21 1723.0 22 1976.5 23 2147.0 24 2460.0 25 2773.0 26 3108.0 27 3443.0 28 3649.5 29 3980.0 30 4104.0 31 4013.5 32 3923.0 33 3632.0 34 3341.0 35 3041.0 36 2741.0 37 2589.0 38 2411.5 39 2386.0 40 2530.0 41 2674.0 42 2881.0 43 3088.0 44 4351.5 45 5615.0 46 7621.0 47 8878.0 48 8129.0 49 7519.5 50 6910.0 51 7072.0 52 7234.0 53 7732.0 54 8342.5 55 8455.0 56 9202.5 57 9950.0 58 11071.5 59 12193.0 60 12098.0 61 12003.0 62 11116.0 63 9517.5 64 8806.0 65 7695.5 66 6585.0 67 5845.0 68 5105.0 69 4446.0 70 3787.0 71 3303.0 72 2312.5 73 1806.0 74 1426.0 75 1046.0 76 819.5 77 593.0 78 447.5 79 233.5 80 165.0 81 104.5 82 44.0 83 27.0 84 10.0 85 7.0 86 4.0 87 2.5 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.020097847807611917 10 0.0 11 0.0011484484461492524 12 0.0 13 0.0 14 0.024691641592208928 15 0.17628683648391025 16 0.0373245744998507 17 0.024117417369134298 18 0.0074649148999701405 19 0.016652502469164162 20 0.02526586581528355 21 0.008613363346119392 22 0.0011484484461492524 23 0.0 24 0.0 25 0.0017226726692238783 26 0.1774352849300595 27 0.032156556492179064 28 0.1728414911454625 29 0.024117417369134298 30 0.03904724716907458 31 0.22567011966832812 32 0.014929829799940281 33 0.1952362358453729 34 0.09934079059191034 35 0.049383283184417856 36 0.1619312309070446 37 0.23715460412982062 38 0.15504054023014907 39 0.36980039966005923 40 0.6000643131129844 41 0.8722465948503572 42 0.21648253209913407 43 0.3611870363139399 44 0.49498128029032773 45 0.11714174150722373 46 0.06948113099202977 47 0.03617612605370145 48 0.046512162069044724 49 0.3370696189448056 50 0.21648253209913407 51 0.3330500493832832 52 0.38185910834462644 53 0.19006821783770128 54 0.11484484461492524 55 0.1688219215839401 56 0.20384959919149231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 174148.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.53327566471324 #Duplication Level Percentage of deduplicated Percentage of total 1 90.25106087851685 65.46205077734281 2 5.907997339387971 8.5705279928844 3 1.6475250674533175 3.5850116964635035 4 0.7099678398923546 2.0598517217597263 5 0.36090586397201485 1.3088842260246827 6 0.23112604767314446 1.0058597597503105 7 0.14741484346157482 0.7484737034508271 8 0.12051592497359395 0.6993131846478072 9 0.08366295994262642 0.5461513682799946 >10 0.47309232837705745 6.270602614211711 >50 0.03187147765946631 1.5257501654444408 >100 0.02788754295203302 3.766695720940963 >500 0.003983934707433289 1.8985186717746165 >1k 0.002987951030574966 2.552308397024247 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 1408 0.8085077060890736 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 1069 0.6138456944667754 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1056 0.6063807795668053 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 807 0.46339894802122333 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 661 0.37956221145232794 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 629 0.3611870363139399 No Hit AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 531 0.3049130624526265 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 469 0.26931116062199967 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 438 0.25151020970668625 No Hit AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 360 0.20672072030686542 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 295 0.16939614580701473 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 250 0.14355605576865654 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 224 0.12862622596871626 No Hit AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 218 0.1251808806302685 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 205 0.11771596573029837 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 203 0.11656751728414913 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 200 0.11484484461492524 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 177 0.10163768748420883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.742242230746262E-4 0.0 0.0 0.0 0.0 7 5.742242230746262E-4 0.0 0.0 0.0 0.0 8 5.742242230746262E-4 0.0 0.0 0.0 0.0 9 5.742242230746262E-4 0.0 0.0 0.0 0.0 10 0.0017226726692238785 0.0 0.0 0.0 0.0 11 0.006316466453820888 0.0 0.0 0.0 0.0 12 0.006890690676895514 0.0 0.0 0.0 0.0 13 0.009187587569194019 0.0 0.0 0.0 0.0 14 0.010910260238417898 0.0 0.0 0.0 0.0 15 0.014929829799940281 0.0 0.0 0.0 0.0 16 0.01665250246916416 0.0 0.0 0.0 0.0 17 0.01952362358453729 0.0 0.0 0.0 0.0 18 0.021820520476835796 0.0 0.0 0.0 0.0 19 0.023543193146059672 0.0 0.0 0.0 0.0 20 0.025840090038358177 0.0 0.0 0.0 0.0 21 0.028136986930656683 0.0 0.0 0.0 0.0 22 0.03043388382295519 0.0 0.0 0.0 0.0 23 0.031008108046029815 0.0 0.0 0.0 0.0 24 0.03387922916140294 0.0 0.0 0.0 0.0 25 0.0373245744998507 0.0 0.0 0.0 0.0 26 0.041344144061373087 0.0 0.0 0.0 0.0 27 0.044789489399820845 0.0 0.0 0.0 0.0 28 0.047086386292119344 0.0 0.0 0.0 0.0 29 0.04766061051519397 0.0 0.0 0.0 0.0 30 0.048234834738268596 0.0 0.0 0.0 0.0 31 0.05225440429979098 0.0 0.0 0.0 0.0 32 0.05225440429979098 0.0 0.0 0.0 0.0 33 0.05225440429979098 0.0 0.0 0.0 0.0 34 0.05282862852286561 0.0 0.0 0.0 0.0 35 0.05282862852286561 0.0 0.0 0.0 0.0 36 0.05282862852286561 0.0 0.0 0.0 0.0 37 0.053402852745940234 0.0 0.0 0.0 0.0 38 0.053402852745940234 0.0 0.0 0.0 0.0 39 0.053402852745940234 0.0 0.0 0.0 0.0 40 0.053402852745940234 0.0 0.0 0.0 0.0 41 0.05397707696901486 0.0 0.0 0.0 0.0 42 0.056273973861313366 0.0 0.0 0.0 0.0 43 0.057996646530537245 0.0 0.0 0.0 0.0 44 0.059719319199761124 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAATCC 20 3.9046397E-4 50.68014 38 GCTCTAA 15 0.007980992 50.680138 39 TATTCCC 15 0.008139304 50.429028 36 TATGATT 15 0.008167482 50.38497 35 TCAAGCC 15 0.008167482 50.38497 35 TTATTCC 25 1.9877385E-5 50.38497 35 CAATACC 25 2.0187752E-5 50.25327 34 CATCACT 15 0.008261934 50.238674 44 CACGATT 95 0.0 50.180397 47 CGTGCAC 20 4.1004198E-4 50.180393 48 CGATTCA 125 0.0 50.180393 49 ACGATTC 125 0.0 50.180393 48 TACGAAG 20 4.1004198E-4 50.180393 47 ATACCCC 15 0.00829994 50.180393 46 CCGATTG 20 4.1062938E-4 50.165844 43 CCGATTC 20 4.1062938E-4 50.165844 43 CTTGAAC 15 0.008309463 50.165844 43 TTCACAT 15 0.008318992 50.151302 45 CTCGAAG 20 4.1121745E-4 50.151302 45 CATATTC 15 0.008385932 50.04975 33 >>END_MODULE