FastQCFastQC Report
Fri 17 Jun 2016
SRR1526297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12374
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC440.35558428963956684No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG320.25860675610150313No Hit
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC180.14546630030709554No Hit
TGTCACGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC170.13738483917892355No Hit
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC140.11314045579440762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTG150.00786583250.58024641
GAAGCTG150.00812649150.1673479
ACCCTGC203.9568738E-450.16734742
TCTCTTT150.00812649150.16734746
GCCGAAT150.00812649150.16734710
GGAGTCC203.9568738E-450.1673479
GGAGAGT204.0378733E-449.9634138
GGGGCGT150.00825920349.9634138
CGACTCA150.00825920349.96341332
CGGGGGG1200.049.7611356
TGTCTCC150.008393520549.76113526
CGGGGAG600.049.7611356
CGGGCTG150.008393520549.7611356
CGGGTTC150.008393520549.7611356
GGGAGCT150.008393520549.7611357
CGGGCAG150.008393520549.7611356
CGGGAGA309.978612E-749.7611356
ACGGGTG309.978612E-749.7611355
GTCTCCG150.008393520549.76113527
ACGGGGT600.049.7611355
Produced by FastQC (version 0.11.5)