##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12374
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.46985614999192	39.0	38.0	39.0	33.0	40.0
2	36.68942944884435	39.0	36.0	39.0	31.0	40.0
3	36.946258283497656	39.0	38.0	39.0	32.0	40.0
4	36.92799418134799	39.0	38.0	39.0	32.0	40.0
5	34.9726038467755	39.0	35.0	39.0	20.0	40.0
6	36.91870050105059	38.0	38.0	39.0	33.0	40.0
7	36.610635202844676	38.0	36.0	39.0	32.0	40.0
8	36.62437368676257	38.0	36.0	39.0	31.0	40.0
9	36.710845320834004	38.0	36.0	39.0	32.0	40.0
10	36.37328268951026	38.0	36.0	39.0	31.0	40.0
11	35.37966704380152	38.0	34.0	39.0	29.0	40.0
12	33.229109422983676	37.0	31.0	39.0	20.0	40.0
13	32.49943429772103	36.0	30.0	39.0	17.0	40.0
14	32.31558105705511	36.0	29.0	39.0	16.0	40.0
15	32.85243251979958	37.0	30.0	39.0	18.0	40.0
16	32.59641183125909	36.0	30.0	38.0	19.0	40.0
17	32.544852109261356	36.0	30.0	38.0	19.0	40.0
18	32.537417165023435	36.0	30.0	38.0	19.0	40.0
19	33.55293357038953	36.0	31.0	38.0	24.0	40.0
20	33.83845159204784	36.0	31.0	38.0	25.0	40.0
21	35.20050105058995	38.0	35.0	39.0	27.0	40.0
22	34.80200420235979	38.0	34.0	39.0	26.0	40.0
23	35.048650395991594	38.0	34.0	39.0	27.0	40.0
24	34.62994989494101	38.0	33.0	39.0	26.0	40.0
25	34.96217876192016	38.0	34.0	39.0	27.0	40.0
26	35.25585905931793	38.0	35.0	39.0	28.0	40.0
27	35.21359301761758	38.0	35.0	39.0	28.0	40.0
28	35.26337481816712	38.0	35.0	39.0	28.0	40.0
29	34.99757556166155	38.0	35.0	39.0	27.0	40.0
30	34.94520769355099	38.0	35.0	39.0	27.0	40.0
31	34.68352998222078	38.0	35.0	39.0	26.0	40.0
32	33.512607079359945	38.0	33.0	39.0	19.0	40.0
33	34.90003232584451	38.0	35.0	39.0	27.0	40.0
34	34.64376919347018	38.0	35.0	39.0	26.0	40.0
35	34.682641021496686	38.0	35.0	39.0	27.0	40.0
36	32.59043155002424	35.0	31.0	38.0	23.0	39.0
37	32.25529335703895	35.0	31.0	38.0	22.0	39.0
38	31.57281396476483	35.0	31.0	38.0	17.0	39.0
39	29.437368676256668	34.0	27.0	38.0	6.0	39.0
40	25.069338936479717	31.0	11.0	37.0	4.0	39.0
41	21.627121383546147	25.0	9.0	33.0	4.0	37.0
42	25.599806044932922	30.0	20.0	34.0	4.0	37.0
43	21.77315338613221	26.0	8.0	33.0	4.0	37.0
44	22.807499595926945	28.0	9.0	33.0	4.0	37.0
45	25.242847906901567	30.0	19.0	34.0	4.0	37.0
46	25.319783416841766	30.0	14.0	36.0	4.0	38.0
47	24.549054469048002	30.0	9.0	36.0	4.0	38.0
48	24.532568288346532	30.0	10.0	35.0	4.0	38.0
49	22.002181994504607	27.0	5.0	35.0	4.0	38.0
50	24.33368352998222	30.0	4.0	35.0	4.0	38.0
51	25.716583158235007	32.0	4.0	38.0	4.0	39.0
52	25.497333117827704	32.0	4.0	38.0	4.0	39.0
53	25.152173913043477	32.0	4.0	38.0	4.0	39.0
54	24.152173913043477	31.0	4.0	38.0	4.0	39.0
55	24.4022951349604	31.0	4.0	37.0	4.0	39.0
56	23.37732342007435	30.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.0374059372532969
1	2	1.962833658479454
1	3	1.7129184393091066
1	4	1.6647909526002564
1	5	3.6777896706932935
1	6	1.74158561153844
1	7	2.133641452738317
1	8	1.958536742840593
1	9	2.1088766137303594
1	10	1.94461073296673
1	11	-3.7851751475648605
1	12	-9.243112357798257
1	13	-22.417279515235876
1	14	-20.556179808457614
1	15	-14.161114662960902
1	16	-22.70832606888233
1	17	-23.235042264557922
1	18	-21.421045643372203
1	19	-6.699809723287984
1	20	-11.80091723854672
1	21	-7.058447281411635
1	22	-4.706170088968996
1	23	-6.792956203477306
1	24	-6.197846993781301
1	25	-5.866339173016744
1	26	0.08896700888388409
1	27	-0.38427696953750967
1	28	-0.17129743362160355
1	29	-0.4677931495011194
1	30	0.13391076060672447
1	31	0.6337938754730672
1	32	-10.926780446985052
1	33	-0.7492098025530041
1	34	-0.4281590825580608
1	35	-0.3373527020010343
1	36	2.798026044743594
1	37	4.191316331349103
1	38	4.465761580349181
1	39	7.108985986661828
1	40	9.011699014522716
1	41	-3.397346690323033
1	42	-8.16605535183062
1	43	-14.739053708262471
1	44	-16.3684917403036
1	45	-16.88484810219145
1	46	-17.226973203900148
1	47	-17.32542516292436
1	48	-16.328487384371336
1	49	-13.911302853157078
1	50	-15.550070410431665
1	51	-16.82436956712772
1	52	-17.324933893010932
1	53	-16.53676613457703
1	54	-17.030112589409264
1	55	-16.282646111910022
1	56	-14.674471008442389
2	1	1.148517048364404
2	2	2.073944769590561
2	3	1.7684739948646637
2	4	1.1092353970446993
2	5	3.8166785595821793
2	6	1.79714116709399
2	7	2.07808589718276
2	8	2.347425631729486
2	9	2.331098835952581
2	10	1.4723885107445085
2	11	-3.4796195920093034
2	12	-11.576445691131589
2	13	-14.583946181902544
2	14	-16.22284647512428
2	15	-9.216670218516459
2	16	-6.819437179993443
2	17	-3.1239311534468115
2	18	-12.615490087816646
2	19	-5.366476389954652
2	20	-4.995361682991167
2	21	1.0248860519216976
2	22	1.293829911031004
2	23	-0.07073398125508845
2	24	1.4688196728853669
2	25	-0.8107836174611904
2	26	0.22785589777276982
2	27	0.8379452526847118
2	28	0.9953692330450608
2	29	-0.24557092727889795
2	30	0.661688538384503
2	31	-6.0050950134158185
2	32	-18.510113780318385
2	33	-5.582543135886336
2	34	-4.233714638113614
2	35	-4.809574924223256
2	36	3.3258038225213724
2	37	3.3024274424602176
2	38	4.6046504692380665
2	39	5.33120820888405
2	40	4.6783656811893835
2	41	5.9359866430103025
2	42	1.3061668703916034
2	43	0.8165018472930861
2	44	-9.007380629192488
2	45	0.5318185644752162
2	46	-14.671417648344594
2	47	-14.075425162924361
2	48	-10.939598495482446
2	49	-12.911302853157078
2	50	-10.466737077098333
2	51	-10.407702900461056
2	52	-10.630489448566488
2	53	-9.675655023465918
2	54	-16.69677925607593
2	55	-12.254868334132246
2	56	-11.341137675109056
3	1	1.904072603919964
3	2	2.879500325146118
3	3	2.8962517726424437
3	4	2.4814576192669193
3	5	4.494456337359956
3	6	2.558252278205103
3	7	2.9003081194049827
3	8	2.64187007617393
3	9	3.125543280397025
3	10	2.8612773996333942
3	11	2.33149151910181
3	12	2.0235543088684125
3	13	-3.7339461819025423
3	14	-2.6728464751242775
3	15	-1.3111146629609003
3	16	3.0083405977843327
3	17	3.581624402108744
3	18	2.828954356627797
3	19	2.5501902767120157
3	20	0.9824160947866147
3	21	4.058219385255036
3	22	3.5938299110310012
3	23	4.140377129856027
3	24	3.8188196728853683
3	25	4.06699416031659
3	26	3.338967008883884
3	27	3.0490563637958275
3	28	3.3287025663783965
3	29	4.065540183832219
3	30	3.8172440939400616
3	31	4.217127208806403
3	32	2.9398862196816182
3	33	4.23412353078033
3	34	4.755174250775276
3	35	4.562647297998964
3	36	6.714692711410258
3	37	7.141316331349106
3	38	8.415761580349184
3	39	10.525652653328493
3	40	12.561699014522713
3	41	14.319319976343632
3	42	11.617277981502717
3	43	5.4109462917375275
3	44	8.898174926363069
3	45	11.515151897808547
3	46	3.8563601294331846
3	47	0.5912415037423067
3	48	4.271512615628666
3	49	-7.311302853157079
3	50	3.7832629229016668
3	51	1.0589637662056113
3	52	1.2250661069890683
3	53	1.0632338654229727
3	54	-6.996779256075932
3	55	-2.2993127785766916
3	56	-0.6411376751090572
4	1	1.8707392705866255
4	2	2.367595563241359
4	3	2.189108915499588
4	4	1.640981428790731
4	5	4.475408718312337
4	6	1.8130141829670094
4	7	2.538403357500222
4	8	2.149012933316783
4	9	2.132686137539878
4	10	1.3136583520143432
4	11	1.8695867571970481
4	12	0.8997447850588856
4	13	3.6779585800022154
4	14	1.3962011439233422
4	15	5.267456765610529
4	16	5.529769169212905
4	17	5.884005354489695
4	18	6.3527638804373225
4	19	4.562095038616775
4	20	3.9014637138342323
4	21	3.8106003376359823
4	22	3.43668705388815
4	23	3.4689485584274564
4	24	3.8973911014567975
4	25	4.157470350792778
4	26	3.17230034221722
4	27	1.091913506652972
4	28	2.400131137806966
4	29	3.056016374308406
4	30	2.169625046321009
4	31	3.5980795897587825
4	32	3.3470290768244695
4	33	2.750790197446996
4	34	2.607555203156224
4	35	3.6983615837132504
4	36	3.7861212828388275
4	37	5.929411569444344
4	38	5.525285389872991
4	39	7.347081224757062
4	40	8.98788949071319
4	41	6.98360569062935
4	42	8.13156369578843
4	43	8.094279625070861
4	44	8.833889212077352
4	45	8.865151897808548
4	46	8.606360129433185
4	47	9.448384360885164
4	48	9.981036425152475
4	49	6.612506670652447
4	50	8.342786732425477
4	51	8.878011385253231
4	52	7.7583994403224
4	53	7.094186246375351
4	54	7.707982648685974
4	55	9.729258649994737
4	56	10.492195658224276
5	1	2.204072603919961
5	2	1.4072781029238968
5	3	2.5462517726424423
5	4	1.2759020637113636
5	5	4.816678559582179
5	6	2.630474500427326
5	7	2.5225303416272027
5	8	2.6807589650628145
5	9	2.497765502619245
5	10	1.19461073296673
5	11	1.2426026302129216
5	12	6.423554308868411
5	13	6.138276040319678
5	14	6.499375747097943
5	15	6.283329781483541
5	16	5.513896153339893
5	17	6.54273551321986
5	18	6.495621023294461
5	19	5.744634721156459
5	20	4.004638317008833
5	21	3.0248860519216976
5	22	3.849385466586561
5	23	3.3737104631893615
5	24	4.024375228440924
5	25	3.7447719380943667
5	26	1.1167447866616627
5	27	1.893500808240269
5	28	1.5509247886006179
5	29	1.8099846282766592
5	30	1.4950218717178387
5	31	0.8282383199175172
5	32	2.323219553014951
5	33	1.306345753002553
5	34	2.4329520285530535
5	35	-0.3095749242232557
5	36	4.825803822521372
5	37	3.8024274424602176
5	38	4.660206024793624
5	39	6.664541542217378
5	40	6.845032347856048
5	41	7.269319976343635
5	42	8.972833537058271
5	43	12.316501847293086
5	44	12.214841593029732
5	45	9.309596342252991
5	46	10.384137907210963
5	47	12.146797059297864
5	48	10.949290393406443
5	49	11.75536381350959
5	50	11.088818478457224
5	51	7.758963766205611
5	52	6.202843884766846
5	53	4.324344976534082
5	54	6.4698874105907365
5	55	6.189576110312199
5	56	4.714417880446501
6	1	1.3040726039199626
6	2	1.7295003251461196
6	3	2.446251772642441
6	4	1.9314576192669222
6	5	4.194456337359959
6	6	2.608252278205107
6	7	2.95030811940498
6	8	2.6918700761739274
6	9	2.7755432803970237
6	10	1.4612773996333956
6	11	1.8314915191018102
6	12	4.973554308868408
6	13	4.816053818097455
6	14	5.027153524875722
6	15	5.1888853370391
6	16	4.108340597784334
6	17	6.531624402108747
6	18	4.0289543566278
6	19	3.3001902767120157
6	20	3.6824160947866105
6	21	2.7082193852550347
6	22	2.8438299110310012
6	23	2.340377129856023
6	24	0.06881967288536828
6	25	0.9669941603165881
6	26	1.1889670088838855
6	27	0.7990563637958275
6	28	-1.5712974336216021
6	29	-0.8344598161677865
6	30	-0.38275590605994125
6	31	-0.48287279119359994
6	32	1.7898862196816125
6	33	0.7841235307803345
6	34	0.1551742507752749
6	35	-0.23735270200103287
6	36	-0.23530728858974115
6	37	0.741316331349104
6	38	-0.9342384196508178
6	39	1.475652653328492
6	40	0.861699014522717
6	41	-4.180680023656365
6	42	1.0672779815027127
6	43	6.1609462917375275
6	44	2.7981749263630675
6	45	1.6151518978085484
6	46	5.156360129433185
6	47	5.591241503742307
6	48	6.221512615628665
6	49	9.23869714684292
6	50	5.883262922901668
6	51	7.408963766205609
6	52	6.9250661069890675
6	53	6.163233865422971
6	54	5.65322074392407
6	55	6.900687221423311
6	56	5.708862324890944
7	1	1.4649421691373519
7	2	2.3034133686243763
7	3	1.915816990033747
7	4	1.2662402279625695
7	5	4.703151989533872
7	6	1.8212957564659717
7	7	2.126395075926723
7	8	2.465783119652187
7	9	2.6668476282231097
7	10	2.448233921372527
7	11	3.6314915191018073
7	12	5.206163004520583
7	13	6.4812712094018075
7	14	6.559762220527894
7	15	5.925841858778231
7	16	6.610514510827812
7	17	5.062059184717441
7	18	5.741997834888664
7	19	5.8436685375815784
7	20	4.891111746960526
7	21	3.51039329829851
7	22	3.946003824074481
7	23	1.9099423472473305
7	24	3.034037064189718
7	25	2.619168073360065
7	26	2.1433148349708375
7	27	2.383838972491475
7	28	3.915659088117529
7	29	0.6481488794843884
7	30	2.5650701808965835
7	31	1.3475619914150982
7	32	2.3159731762033573
7	33	1.2580365742585897
7	34	2.9160438159926656
7	35	1.5865603414772238
7	36	1.127736189671129
7	37	1.734794592218666
7	38	2.8027181020883134
7	39	3.8626091750676217
7	40	0.9899598840879342
7	41	-7.9480713280041915
7	42	5.852060590198366
7	43	7.152250639563615
7	44	8.461218404623938
7	45	6.560804071721591
7	46	7.171577520737532
7	47	10.982545851568393
7	48	10.388903919976492
7	49	11.262610190321183
7	50	8.663697705510362
7	51	9.867659418379525
7	52	9.903326976554286
7	53	11.495842561075143
7	54	11.151046830880592
7	55	10.691991569249396
7	56	9.767557977064858
8	1	0.9099549568611351
8	2	2.3059709133814152
8	3	2.7521341255836163
8	4	2.0667517369139787
8	5	4.506221043242313
8	6	2.0258993370286333
8	7	2.241484589993213
8	8	2.527164193820987
8	9	2.0990726921617266
8	10	2.5377479878686913
8	11	1.8667856367488653
8	12	5.482377838280179
8	13	6.739583229862159
8	14	6.541859407228664
8	15	4.662414748803801
8	16	5.5465758919019805
8	17	5.343389107991101
8	18	3.181895533098384
8	19	3.5060726296531897
8	20	4.047121977139554
8	21	2.4464546793726782
8	22	3.411476969854533
8	23	2.687435953385439
8	24	2.409996143473599
8	25	-0.7388881926245858
8	26	2.956614067707413
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115	35	1.7126472979989629
115	36	1.1146927114102567
115	37	3.7913163313491047
115	38	3.215761580349181
115	39	7.875652653328494
115	40	8.011699014522716
115	41	9.969319976343634
115	42	2.8172779815027127
115	43	4.760946291737529
115	44	-3.351825073636931
115	45	5.365151897808548
115	46	-1.4936398705668168
115	47	-4.108758496257693
115	48	-4.128487384371336
115	49	-6.111302853157078
115	50	-0.9667370770983332
115	51	3.158963766205609
115	52	1.1250661069890668
115	53	2.4132338654229706
115	54	1.2032207439240672
115	55	2.30068722142331
115	56	5.458862324890944
116	1	2.1131635130108677
116	2	3.0385912342370247
116	3	2.955342681733349
116	4	2.7405485283578273
116	5	5.003547246450864
116	6	2.3627977327505576
116	7	2.027580846677708
116	8	3.0191428034466554
116	9	3.0028160076697503
116	10	2.088550126906121
116	11	0.9951278827381742
116	12	0.05991794523204774
116	13	-3.447582545538907
116	14	-7.495573747851552
116	15	-1.3429328447790851
116	16	-5.132568493124754
116	17	-3.5229210524367076
116	18	0.6471361748096136
116	19	0.16382664034838257
116	20	0.00968882205933852
116	21	3.8582193852550333
116	22	3.5665571837582775
116	23	3.7676498571287524
116	24	3.6506378547035467
116	25	2.4215396148620414
116	26	2.5662397361566107
116	27	2.4036018183412793
116	28	3.0105207481965763
116	29	2.410994729286763
116	30	3.1263350030309667
116	31	0.3989453906245828
116	32	-1.782841053045658
116	33	2.993214439871238
116	34	4.109719705320728
116	35	3.321738207089872
116	36	5.941965438682985
116	37	7.327679967712736
116	38	6.079397943985548
116	39	9.957470835146673
116	40	12.602608105431809
116	41	10.360229067252725
116	42	2.5990961633208975
116	43	-7.602690071898834
116	44	-0.4518250736369325
116	45	1.7742428068994585
116	46	-7.393639870566815
116	47	-9.954213041712238
116	48	-7.010305566189517
116	49	-6.638575580429805
116	50	-1.6485552589165167
116	51	2.077145584387427
116	52	1.7887024706254309
116	53	-1.559493407304302
116	54	-3.742233801530478
116	55	1.3006872214233098
116	56	5.88613505216367
117	1	0.7755011753485306
117	2	2.129500325146118
117	3	2.0462517726424423
117	4	1.8314576192669207
117	5	3.3801706230742425
117	6	0.19396656391938905
117	7	1.1574509765478354
117	8	0.8632986476024982
117	9	0.8469718518255931
117	10	0.6469916853476789
117	11	-9.082794195183908
117	12	-9.719302833988731
117	13	-10.369660467616828
117	14	-12.651417903695705
117	15	-6.732543234389475
117	16	-10.184516545072807
117	17	-3.925518455034112
117	18	-1.3139027862293453
117	19	-0.9140954375736996
117	20	-0.2890124766419575
117	21	2.5725050995407486
117	22	3.0081156253167194
117	23	2.4689485584274564
117	24	-4.95975175568606
117	25	-0.46157726825484247
117	26	2.2675384374553147
117	27	1.949056363795826
117	28	2.5429882806641118
117	29	2.8655401838322163
117	30	3.360101236797206
117	31	0.3599843516635488
117	32	-10.652970923175527
117	33	0.36983781649461633
117	34	2.2266028222038443
117	35	1.984075869427535
117	36	0.5004069971245428
117	37	1.548459188491961
117	38	-2.4270955625079615
117	39	4.63279551047135
117	40	-0.6311581283344267
117	41	0.41217711920077704
117	42	-0.5827220184972859
117	43	-8.810482279691042
117	44	-12.737539359351217
117	45	-4.7062766736200246
117	46	-5.536497013423958
117	47	-13.123044210543407
117	48	-7.685630241514193
117	49	-9.911302853157078
117	50	-4.1810227913840485
117	51	-2.4553219480801047
117	52	-1.5749338930109325
117	53	-4.001051848862744
117	54	-6.911064970361645
117	55	-2.69931277857669
117	56	-0.4125662465376294
118	1	0.9818503816977397
118	2	1.9072781029238968
118	3	1.8240295504202209
118	4	1.6092353970446993
118	5	3.3166785595821793
118	6	1.4638078337606615
118	7	1.4114192305160955
118	8	1.847425631729486
118	9	1.7199877248414666
118	10	1.25016628852228
118	11	-3.92406403645375
118	12	-6.465334580020478
118	13	-8.528390626346987
118	14	-0.7784020306798354
118	15	-15.050003551849791
118	16	-4.263881624437886
118	17	-5.512820042335697
118	18	-1.72660119892776
118	19	-0.1998097232879843
118	20	-1.2731394607689452
118	21	2.8582193852550333
118	22	1.8493854665865612
118	23	2.8181549076338044
118	24	-1.3089581048924117
118	25	3.1892163825388096
118	26	3.17230034221722
118	27	2.7268341415736046
118	28	2.4953692330450608
118	29	1.6433179616099949
118	30	2.7727996494956173
118	31	-1.5606505689713757
118	32	-5.843447113651717
118	33	0.8619013085581102
118	34	0.5440631396641606
118	35	-2.031797146445477
118	36	4.770248266965815
118	37	3.6913163313491033
118	38	6.715761580349181
118	39	9.108985986661828
118	40	10.17836568118938
118	41	9.047097754121413
118	42	-1.138277574052843
118	43	-5.794609263818026
118	44	-1.3407139625258218
118	45	-2.5792925466358945
118	46	-4.171417648344594
118	47	-8.07542516292436
118	48	-7.050709606593557
118	49	-8.800191742045968
118	50	-1.6889592993205547
118	51	-3.4077029004610573
118	52	-2.5749338930109325
118	53	-4.953432801243697
118	54	-6.97455703385371
118	55	-4.365979445243358
118	56	-2.618915452886835
119	1	0.5374059372532969
119	2	0.6850558807016753
119	3	1.379585105975778
119	4	1.1647909526002564
119	5	1.650011892915515
119	6	0.9082522782051043
119	7	0.9669747860716456
119	8	0.2918700761739288
119	9	1.2755432803970237
119	10	1.1390551774111728
119	11	-4.92406403645375
119	12	-4.243112357798257
119	13	-1.1950572930136545
119	14	-2.4450686973464997
119	15	-4.605559107405345
119	16	-6.152770513326775
119	17	-7.6239311534468115
119	18	-5.282156754483314
119	19	-2.1998097232879843
119	20	-2.6064727941022774
119	21	-0.47511394807830243
119	22	-0.4839478667467745
119	23	-0.18184509236619562
119	24	-3.642291438225744
119	25	1.1892163825388096
119	26	1.1723003422172198
119	27	1.6157230304624903
119	28	0.49536923304506075
119	29	0.754429072721102
119	30	0.4394663161622816
119	31	0.6615716532508458
119	32	-4.287891558096163
119	33	-0.8047653581085541
119	34	1.4329520285530535
119	35	0.6348695202211871
119	36	5.881359378076922
119	37	6.24687188690466
119	38	5.826872691460295
119	39	8.886763764439607
119	40	9.845032347856051
119	41	8.158208865232524
119	42	0.19505575928049268
119	43	2.316501847293086
119	44	0.4370638152519568
119	45	0.30959634225299126
119	46	-2.0603065372334832
119	47	-8.742091829591027
119	48	-3.4951540510380035
119	49	-3.1335250753793
119	50	0.7554851451238882
119	51	-3.8521473449055
119	52	-4.13048944856649
119	53	-6.286766134577029
119	54	-3.8634459227425992
119	55	-5.365979445243358
119	56	-2.3966932306646136
120	1	-0.5459273960800388
120	2	1.7545003251461182
120	3	-0.5787482273575577
120	4	1.3314576192669207
120	5	2.969456337359958
120	6	1.5332522782051043
120	7	1.1753081194049813
120	8	1.9168700761739288
120	9	0.5255432803970237
120	10	-0.013722600366605775
120	11	-6.743508480898193
120	12	1.173554308868411
120	13	-6.458946181902544
120	14	-6.347846475124278
120	15	-5.161114662960902
120	16	-7.666659402215664
120	17	-10.193375597891254
120	18	-4.921045643372203
120	19	-5.199809723287984
120	20	-2.967583905213388
120	21	-0.7667806147449667
120	22	0.7938299110310041
120	23	-0.7096228701439742
120	24	-3.031180327114633
120	25	-1.0330058396834119
120	26	-0.4110329911161159
120	27	2.574056363795826
120	28	-1.1712974336216035
120	29	-1.2594598161677837
120	30	-2.15775590605994
120	31	-2.907872791193597
120	32	-9.885113780318385
120	33	-7.540876469219668
120	34	-14.219825749224725
120	35	-7.212352702001034
120	36	-3.660307288589742
120	37	-3.6836836686508967
120	38	-2.909238419650819
120	39	0.7756526533284926
120	40	2.1366990145227156
120	41	-2.2306800236563653
120	42	-4.207722018497286
120	43	-3.864053708262471
120	44	-7.4518250736369325
120	45	-13.259848102191452
120	46	-12.393639870566815
120	47	-14.408758496257693
120	48	-11.828487384371336
120	49	-13.536302853157078
120	50	-9.591737077098333
120	51	-10.74103623379439
120	52	-9.574933893010932
120	53	-10.661766134577029
120	54	-10.946779256075931
120	55	-10.69931277857669
120	56	-5.0911376751090565
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	88.0
5	3.0
6	6.0
7	13.0
8	15.0
9	25.0
10	23.0
11	13.0
12	18.0
13	21.0
14	28.0
15	46.0
16	68.0
17	78.0
18	123.0
19	113.0
20	147.0
21	216.0
22	238.0
23	307.0
24	341.0
25	449.0
26	368.0
27	386.0
28	463.0
29	440.0
30	536.0
31	620.0
32	727.0
33	879.0
34	1025.0
35	1260.0
36	1386.0
37	1155.0
38	587.0
39	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.23226119282367	0.6788427347664457	0.08081461128171974	0.008081461128171973
8	99.57976402133507	0.21819945046064326	0.06465168902537578	0.13738483917892355
9	98.45619139993534	1.0184287099903007	0.2828968638861946	0.24248302618816686
10	54.89736544367222	28.390172943268144	8.000646516890255	8.711815096169387
11	34.580572167447876	28.010344270244058	15.904315500242443	21.504768062065622
12	32.56828834653305	14.69209633101665	21.318894456117665	31.420720866332637
13	25.860675610150313	16.162922256343947	24.923226119282365	33.053176014223375
14	21.75424413904608	18.45594179466451	26.103476151980598	33.68633791430881
15	22.457901554404145	19.57577720207254	26.797279792746114	31.1690414507772
16	25.553759094583672	19.094583670169765	26.38641875505255	28.965238480194017
17	25.87712206952304	23.775262732417136	19.814066289409862	30.53354890864996
18	29.84157775622373	24.854510184287097	18.98642095053346	26.317491108955704
19	26.99054239754264	26.12561636084391	19.14154070002425	27.7423005415892
20	30.017790716480675	23.038977842471294	20.34611030244218	26.597121138605857
21	27.808307742039762	23.06449005980281	22.902860837239373	26.224341360918054
22	27.800226280911588	22.531113625343462	23.75141425569743	25.91724583804752
23	28.083077420397608	25.34346209794731	24.203976078875062	22.369484402780024
24	25.60206885404881	24.72927105220624	22.183610796832067	27.485049296912884
25	24.890900274769677	26.83853240665912	23.121060287700015	25.14950703087118
26	24.412955465587043	25.88663967611336	26.12145748987854	23.57894736842105
27	23.95698576972833	20.68240620957309	26.96474773609314	28.395860284605433
28	21.954972465176546	22.602850664075152	29.51085195983155	25.931324910916747
29	21.172190784155216	20.767987065481	30.90541632983023	27.15440582053355
30	19.34831824062096	22.93014230271669	33.974773609314354	23.746765847347994
31	21.19248217757615	23.35547634478289	35.42611795204148	20.02592352559948
32	23.528460543337644	19.647477360931436	35.26034928848642	21.5637128072445
33	23.236984859525545	18.91344830378107	36.750060723828035	21.099506112865356
34	22.4739098778416	20.046921770083326	39.446646711431114	18.03252164064396
35	21.68216740800647	22.51516376870198	39.81399110392236	15.988677719369187
36	21.082874716736807	19.96600841696342	38.353836192942694	20.597280673357073
37	21.641549181656135	22.03856749311295	37.24679954626479	19.073083778966133
38	23.210236475542597	23.485584710074505	38.11143505021056	15.192743764172336
39	20.987754440029192	18.83058957099992	34.66872110939907	25.512934879571812
40	12.724465751198505	15.34898838059641	27.423417567238157	44.503128300966935
41	15.718818647791066	17.606378651045482	29.924334879179888	36.75046782198357
42	19.439267482375822	23.05323717689004	39.2836885179483	18.223806822785836
43	11.397535667963684	14.259079118028534	30.577172503242544	43.76621271076524
44	13.053366907643571	15.344001299650719	29.234018357566406	42.36861343513931
45	19.20679886685552	20.93889113719142	39.506272764063134	20.348037231889922
46	19.348054679284964	20.488554557955187	42.247027420529	17.916363342230852
47	18.004689142210363	19.597380548144557	38.2892715660118	24.108658743633278
48	19.232012934519	22.635408245755862	39.781729991915924	18.350848827809216
49	14.298451811623572	15.749371808381293	28.726594796141686	41.22558158385345
50	16.628849270664507	22.22852512155592	43.36304700162075	17.779578606158832
51	21.78426601784266	22.49797242497972	37.364152473641525	18.35360908353609
52	19.540882543802727	23.669695003244644	37.78390655418559	19.005515898767033
53	18.87709632990359	25.76359069918172	38.52385967755003	16.83545329336466
54	17.675623179022338	22.466817740369052	33.352217546131435	26.50534153447718
55	20.73990123856553	22.229417955152595	39.56124018457055	17.469440621711325
56	19.044534412955468	23.554655870445345	37.10121457489878	20.299595141700404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	3.0
12	2.5
13	16.5
14	31.0
15	51.5
16	72.0
17	87.0
18	102.0
19	104.0
20	106.0
21	120.0
22	145.0
23	156.0
24	187.0
25	218.0
26	272.0
27	326.0
28	391.0
29	495.0
30	534.0
31	530.5
32	527.0
33	492.5
34	458.0
35	410.0
36	362.0
37	312.5
38	247.5
39	232.0
40	224.5
41	217.0
42	206.5
43	196.0
44	182.5
45	169.0
46	177.5
47	202.5
48	219.0
49	235.0
50	251.0
51	272.0
52	293.0
53	324.5
54	406.0
55	456.0
56	484.5
57	513.0
58	589.0
59	665.0
60	678.5
61	692.0
62	733.5
63	764.5
64	754.0
65	741.0
66	728.0
67	666.0
68	604.0
69	531.5
70	459.0
71	382.5
72	262.0
73	218.0
74	181.5
75	145.0
76	117.0
77	89.0
78	78.0
79	41.5
80	16.0
81	15.5
82	15.0
83	8.0
84	1.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.016162922256343946
10	0.0
11	0.0
12	0.0
13	0.0
14	0.03232584451268789
15	0.17779214481978342
16	0.03232584451268789
17	0.03232584451268789
18	0.016162922256343946
19	0.02424438338451592
20	0.06465168902537578
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.19395506707612736
27	0.04848876676903184
28	0.2101179893324713
29	0.03232584451268789
30	0.04848876676903184
31	0.2424438338451592
32	0.04848876676903184
33	0.18587360594795538
34	0.10505899466623565
35	0.07273315015354777
36	0.14546630030709554
37	0.25860675610150313
38	0.2101179893324713
39	0.34750282851139486
40	0.5414578955875222
41	0.6707612736382738
42	0.2666882172296751
43	0.307095522870535
44	0.5091320510748344
45	0.1535477614352675
46	0.08889607240989171
47	0.04040730564085987
48	0.03232584451268789
49	0.299014061742363
50	0.2747696783578471
51	0.35558428963956684
52	0.37174721189591076
53	0.2505252949733312
54	0.14546630030709554
55	0.16971068369161144
56	0.19395506707612736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	12374.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.27202198157427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.68744466088188	86.4231453046711
2	3.7896228085709227	6.917730725715209
3	0.734903488578006	2.0122838209148215
4	0.318753320347087	1.1637304024567643
5	0.16823091907207366	0.7677388071763375
6	0.1416681423764831	0.7758202683045095
7	0.03541703559412077	0.22628091158881525
8	0.026562776695590578	0.19395506707612736
9	0.017708517797060386	0.14546630030709554
>10	0.07968833008677174	1.3738483917892355
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	44	0.35558428963956684	No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	32	0.25860675610150313	No Hit
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC	18	0.14546630030709554	No Hit
TGTCACGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC	17	0.13738483917892355	No Hit
TGTCACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC	14	0.11314045579440762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.008081461128171973	0.0	0.0	0.0	0.0
14	0.008081461128171973	0.0	0.0	0.0	0.0
15	0.008081461128171973	0.0	0.0	0.0	0.0
16	0.02424438338451592	0.0	0.0	0.0	0.0
17	0.03232584451268789	0.0	0.0	0.0	0.0
18	0.04848876676903184	0.0	0.0	0.0	0.0
19	0.04848876676903184	0.0	0.0	0.0	0.0
20	0.07273315015354777	0.0	0.0	0.0	0.0
21	0.08081461128171974	0.0	0.0	0.0	0.0
22	0.08889607240989171	0.0	0.0	0.0	0.0
23	0.08889607240989171	0.0	0.0	0.0	0.0
24	0.10505899466623565	0.0	0.0	0.0	0.0
25	0.11314045579440762	0.0	0.0	0.0	0.0
26	0.12122191692257961	0.0	0.0	0.0	0.0
27	0.12122191692257961	0.0	0.0	0.0	0.0
28	0.12122191692257961	0.0	0.0	0.0	0.0
29	0.12122191692257961	0.0	0.0	0.0	0.0
30	0.12122191692257961	0.0	0.0	0.0	0.0
31	0.12122191692257961	0.0	0.0	0.0	0.0
32	0.12122191692257961	0.0	0.0	0.0	0.0
33	0.12122191692257961	0.0	0.0	0.0	0.0
34	0.12122191692257961	0.0	0.0	0.0	0.0
35	0.12122191692257961	0.0	0.0	0.0	0.0
36	0.12122191692257961	0.0	0.0	0.0	0.0
37	0.12122191692257961	0.0	0.0	0.0	0.0
38	0.12122191692257961	0.0	0.0	0.0	0.0
39	0.12122191692257961	0.0	0.0	0.0	0.0
40	0.12122191692257961	0.0	0.0	0.0	0.0
41	0.12122191692257961	0.0	0.0	0.0	0.0
42	0.12122191692257961	0.0	0.0	0.0	0.0
43	0.12930337805075157	0.0	0.0	0.0	0.0
44	0.12930337805075157	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCCTG	15	0.007865832	50.580246	41
GAAGCTG	15	0.008126491	50.167347	9
ACCCTGC	20	3.9568738E-4	50.167347	42
TCTCTTT	15	0.008126491	50.167347	46
GCCGAAT	15	0.008126491	50.167347	10
GGAGTCC	20	3.9568738E-4	50.167347	9
GGAGAGT	20	4.0378733E-4	49.963413	8
GGGGCGT	15	0.008259203	49.963413	8
CGACTCA	15	0.008259203	49.963413	32
CGGGGGG	120	0.0	49.761135	6
TGTCTCC	15	0.0083935205	49.761135	26
CGGGGAG	60	0.0	49.761135	6
CGGGCTG	15	0.0083935205	49.761135	6
CGGGTTC	15	0.0083935205	49.761135	6
GGGAGCT	15	0.0083935205	49.761135	7
CGGGCAG	15	0.0083935205	49.761135	6
CGGGAGA	30	9.978612E-7	49.761135	6
ACGGGTG	30	9.978612E-7	49.761135	5
GTCTCCG	15	0.0083935205	49.761135	27
ACGGGGT	60	0.0	49.761135	5
>>END_MODULE