FastQCFastQC Report
Fri 17 Jun 2016
SRR1526296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74178
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3190.4300466445576856No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2560.3451158025290517No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2310.311413087438324No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1780.23996333144598128No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1430.1927795303189625No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1240.16716546685000944No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1230.16581735824638033No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG950.1280703173447653No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC900.12132977432661976No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC880.11863355711936154No Hit
CCATCTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC880.11863355711936154No Hit
CCATCTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT760.10245625387581224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCAA150.00814080950.39768240
ACAAACC150.00816280250.36332738
GCTCATA150.00816280250.36332741
AACAACA150.00825121750.22637642
CGATTAA600.050.22637649
GCCAGCT150.00825121750.22637642
GTCTCCA252.0137606E-550.2263742
GTGCGCT252.0137606E-550.2263749
ACAACAC150.00827343250.19225343
CTCCACG301.0074236E-650.19225344
CCGACAC204.080227E-450.19225343
CAACGAT507.2759576E-1250.1581846
GTCCTCA252.0300373E-550.1581846
TATATTT150.00829569150.1581846
CACGATT301.0121621E-650.1581847
TTATATT150.00829569150.1581845
ACGATTA600.050.1581848
AACGATT507.2759576E-1250.1581847
TTGAAAC150.00829569150.1581848
CCACGAT204.0939398E-450.15817646