FastQCFastQC Report
Fri 17 Jun 2016
SRR1526295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9634
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA400.4151961801951422No Hit
CGTGGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC280.29063732613659954No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAACCAAAAAAAAAAAAAAA250.25949761262196386No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAACCAAAACAAAAAAA210.21797799460244968No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAACCAAAAAAAAAAAAAAA210.21797799460244968No Hit
CGTGGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC200.2075980900975711No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACAAAAAAAAAAAAAAAA140.14531866306829977No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACAAAAAAAAAAAAAAA110.1141789495536641No Hit
CGTGGCGGGGAGACCACACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA100.10379904504878555No Hit
CGTGGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC100.10379904504878555No Hit
CGTGGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC100.10379904504878555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAC150.00779444750.60846743
CAACACA150.00779444750.60846744
CTTCTCT203.7448306E-450.60846744
GAGTGCA150.00812830850.07853710
GAAGCTG150.00812830850.0785379
ATGGTAT150.00812830850.07853714
TCGTCTT150.00812830850.07853711
GTCTTAA150.00812830850.07853713
CTTAATC203.946683E-450.07853715
CGTCTTA150.00812830850.07853712
TCTTAAT150.00812830850.07853714
AAGCATG251.9168248E-550.07853710
GAAGCAT354.5318302E-850.0785339
GATGGGG150.00829917249.8177125
GCGGGGT402.3101165E-949.817715
GGATGTC150.00829917249.817718
GCGGGCT505.456968E-1249.817715
GCGGGAG451.1277734E-1049.817715
GCGGGAC451.1277734E-1049.817715
GGATGGC204.0508E-449.817718
Produced by FastQC (version 0.11.5)