FastQCFastQC Report
Fri 17 Jun 2016
SRR1526294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18663
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC570.30541713550876065No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC530.2839843540695494No Hit
ATCTCTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC330.176820446873493No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC270.14467127471467608No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC270.14467127471467608No Hit
ATCTCTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC220.117880297915662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTGC150.00797367650.5108739
GCAGCAT150.00797367650.5108742
CTTGCCT203.877195E-450.51086841
TTGCCTA203.877195E-450.51086842
CCGCTGC150.00806001950.37398543
AGACCAC150.00806001950.37398537
CGCTGCA150.00814705750.2378444
CGACAAG150.00814705750.2378449
GACAGTG150.00832322849.9677439
CGCCTGG150.00832322849.96774334
TGCTGTG150.00832322849.96774313
GGACATG150.00832322849.9677438
GGACAGT204.0908187E-449.9677438
CTGGTGA204.0908187E-449.96774313
GTCCTTT150.00832322849.9677439
GCTGTGC150.00832322849.96774314
GGCTTTT150.00832322849.9677439
GTCTTAA309.972828E-749.96774313
GGAAGTG204.0908187E-449.9677438
TGGTGAG204.0908187E-449.96774314