FastQCFastQC Report
Fri 17 Jun 2016
SRR1526293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32536
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT600.1844111138431276No Hit
ATGGCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA490.15060240963855423No Hit
ATGGCGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT480.14752889107450207No Hit
ATGGCGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC440.13523481681829358No Hit
ATGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA430.13216129825424147No Hit
ATGGCGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT410.1260142611261372No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC350.10757314974182443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCA150.007943233550.64576739
TTCATTT150.00799267750.5665138
ACAAACC203.8997046E-450.5665138
CAACCTG150.00799267750.5665141
CAACACA354.82014E-850.33021544
CACGTGG204.0220353E-450.2519543
CGTGGCT204.0220353E-450.2519545
TCACGTG204.0220353E-450.2519542
AGACTCT204.0220353E-450.2519537
TGGCTGA204.0220353E-450.2519547
ACAAAAC309.815249E-750.25194548
CTGAGTC505.456968E-1250.25194549
ATCTCCA150.00819274450.25194545
GTTCTGA505.456968E-1250.25194546
CCCACAC150.00819274450.25194543
CCCGTCG150.00819274450.25194542
CCCAACA150.00819274450.25194543
CCCCAAC150.00819274450.25194542
AGTTCTG505.456968E-1250.25194545
ACAACAC150.00819274450.25194543