FastQCFastQC Report
Fri 17 Jun 2016
SRR1526292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70353
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2810.3994143817605504No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2060.29280911972481627No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1230.1748326297386039No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1190.16914701576336474No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG990.140718945887169No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC890.1265049109490711No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG810.1151336829985928No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC750.10660526203573409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCT150.00816784850.3527341
CGATTAA355.0549716E-850.17251649
AAGTGCC150.00828454850.17251234
TACCAAG252.0341276E-550.13662747
CAACGAT402.557499E-950.13662746
AACGATT402.557499E-950.13662747
CCGACCG252.0341276E-550.13662744
CTACACG150.00830803650.13662346
CGACTCC150.00830803650.13662344
CTCTCAC150.00830803650.13662347
GCCCGCG150.00830803650.13662346
TCGCCTA252.0427646E-550.1007942
ACCTATT150.00833157450.1007942
TTAGTTG150.00833157450.1007942
TGTAGCG150.00833157450.1007942
CACAATT150.00833157450.1007942
CGCCTAG204.1302538E-450.06543
CCTATTC150.00835516150.06543
ATGATTT150.00837879750.02926633
CATGATT150.008402484549.99358432