##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526292_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70353 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.63583642488593 39.0 38.0 39.0 34.0 40.0 2 37.26756499367475 39.0 38.0 39.0 33.0 40.0 3 36.38763094679687 38.0 36.0 39.0 30.0 40.0 4 35.7810327917786 39.0 37.0 39.0 27.0 40.0 5 37.08707517803079 39.0 38.0 39.0 32.0 40.0 6 36.69458303128509 38.0 36.0 39.0 31.0 40.0 7 37.080749932483336 38.0 37.0 39.0 33.0 40.0 8 36.905036032578565 38.0 37.0 39.0 33.0 40.0 9 36.99766889827015 38.0 37.0 39.0 33.0 40.0 10 36.213480590735294 38.0 35.0 39.0 30.0 40.0 11 34.55399201171237 37.0 32.0 39.0 27.0 40.0 12 32.48801046152971 35.0 29.0 39.0 18.0 40.0 13 32.335010589456026 36.0 29.0 39.0 16.0 40.0 14 31.635523716117294 36.0 28.0 39.0 14.0 40.0 15 32.397808195812544 36.0 30.0 39.0 16.0 40.0 16 32.10026580245334 36.0 29.0 39.0 17.0 40.0 17 32.27219876906457 36.0 29.0 38.0 18.0 40.0 18 32.494818984265066 36.0 29.0 38.0 19.0 40.0 19 33.346040680567995 36.0 30.0 38.0 23.0 40.0 20 33.235768197518226 36.0 30.0 39.0 23.0 40.0 21 34.88468153454721 38.0 34.0 39.0 27.0 40.0 22 34.60941253393601 38.0 33.0 39.0 25.0 40.0 23 34.78283797421574 38.0 33.0 39.0 26.0 40.0 24 34.356487996247495 38.0 33.0 39.0 24.0 40.0 25 34.594118232342616 38.0 33.0 39.0 26.0 40.0 26 34.93916393046494 38.0 35.0 39.0 27.0 40.0 27 34.94196409534775 38.0 35.0 39.0 27.0 40.0 28 34.756271942916435 38.0 34.0 39.0 27.0 40.0 29 34.648955979133795 38.0 34.0 39.0 27.0 40.0 30 34.37661506971984 38.0 33.0 39.0 26.0 40.0 31 33.95427344960414 38.0 33.0 39.0 24.0 40.0 32 32.9526530496212 37.0 32.0 39.0 17.0 40.0 33 34.14256677042912 38.0 33.0 39.0 25.0 40.0 34 33.72139070117834 37.0 33.0 39.0 23.0 40.0 35 33.7985302687874 37.0 33.0 39.0 24.0 40.0 36 31.609639958495016 35.0 30.0 38.0 19.0 39.0 37 31.386493824001818 35.0 30.0 38.0 17.0 39.0 38 30.673048768353873 34.0 30.0 38.0 10.0 39.0 39 28.828834591275424 33.0 26.0 37.0 4.0 39.0 40 24.674228533253736 30.0 11.0 36.0 4.0 39.0 41 21.16687277017327 24.0 8.0 32.0 4.0 36.0 42 24.81820249314173 29.0 19.0 33.0 4.0 36.0 43 20.820718377325772 24.0 7.0 32.0 4.0 36.0 44 21.84269327534007 26.0 8.0 33.0 4.0 36.0 45 24.238326723807088 29.0 17.0 33.0 4.0 36.0 46 24.125083507455262 29.0 4.0 35.0 4.0 38.0 47 23.530808920728326 29.0 4.0 35.0 4.0 38.0 48 23.486432703651584 28.0 4.0 35.0 4.0 38.0 49 21.019999147157904 25.0 4.0 34.0 4.0 38.0 50 23.207382769746847 28.0 4.0 34.0 4.0 38.0 51 24.821187440478727 31.0 4.0 37.0 4.0 39.0 52 24.63660398277259 31.0 4.0 37.0 4.0 39.0 53 24.204724745213422 31.0 4.0 36.0 4.0 39.0 54 23.411638451807313 30.0 4.0 36.0 4.0 39.0 55 23.55858314499737 30.0 4.0 36.0 4.0 39.0 56 22.999900501755434 30.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.142873188612164 1 2 1.7457692967229548 1 3 2.4190176701074932 1 4 3.2673795333559497 1 5 1.7820712235101865 1 6 2.2115245246328996 1 7 1.645738012518585 1 8 1.8154616126078622 1 9 1.6290371231963476 1 10 2.1399012985490344 1 11 -4.684624063268686 1 12 -7.122522199788577 1 13 -21.264016427921927 1 14 -17.671052997146308 1 15 -11.55300467454995 1 16 -20.566185256913293 1 17 -21.411608382281912 1 18 -20.419538638442138 1 19 -6.171192531259766 1 20 -10.84769824514547 1 21 -8.427226456058243 1 22 -2.8998932555750443 1 23 -3.309486689723542 1 24 -3.6006054310268745 1 25 -3.823475134088511 1 26 2.7713752449364435 1 27 2.354947884514104 1 28 2.2433367874122894 1 29 1.8637374106729965 1 30 2.709968845064232 1 31 0.6146946506281594 1 32 -12.362661825102123 1 33 0.7038588583348613 1 34 0.2398168471992861 1 35 0.529129814763337 1 36 3.5659698328323266 1 37 3.331971857134125 1 38 4.196203034711424 1 39 5.878852550141492 1 40 7.681509011391196 1 41 -8.232301394109742 1 42 -11.975044518423552 1 43 -13.638638718740403 1 44 -15.397616845163041 1 45 -16.192193847120883 1 46 -17.910298088427147 1 47 -17.238726364230466 1 48 -16.59468041485459 1 49 -14.511666046592378 1 50 -16.33860328356037 1 51 -17.998969594367697 1 52 -17.759773328952974 1 53 -17.627470298655076 1 54 -17.160635801620977 1 55 -17.056613149559187 1 56 -16.380832423273816 2 1 0.9088678122680776 2 2 1.4364951031745719 2 3 2.07264670236556 2 4 2.991035447334447 2 5 1.361506707381153 2 6 1.8552073203318287 2 7 1.3164369372497688 2 8 1.4664024728229208 2 9 1.369225295239353 2 10 1.7619443093017253 2 11 -4.205322987999871 2 12 -10.999538328820837 2 13 -14.383640083835907 2 14 -14.683418588544159 2 15 -9.909321878851024 2 16 -4.549249773042327 2 17 -3.278006231744275 2 18 -11.756232186829234 2 19 -3.0121871549156793 2 20 -4.121757384930419 2 21 2.382585371898749 2 22 3.121746529371194 2 23 2.2690079339323717 2 24 2.439313923811838 2 25 2.4070356185996644 2 26 2.052154814828917 2 27 2.3580392823635705 2 28 2.3371539917133646 2 29 2.545323432178371 2 30 2.0746193826986428 2 31 -1.3444451343180752 2 32 -8.194785481016101 2 33 -0.6575658728479326 2 34 -0.8900218624781324 2 35 -0.13874652932268816 2 36 4.461668757563512 2 37 4.656165405521218 2 38 5.582896583098517 2 39 7.378986958743642 2 40 9.850191807090123 2 41 5.34619322954617 2 42 0.8871866643721482 2 43 -2.7961656004608333 2 44 -9.788611468818955 2 45 -0.07687126647572384 2 46 -11.08381959380349 2 47 -10.598538192187457 2 48 -8.594277189048139 2 49 -10.767714433689152 2 50 -8.29868392872166 2 51 -8.625985723399955 2 52 -8.60910128594222 2 53 -9.32155632016045 2 54 -14.239130425276889 2 55 -9.89223142912908 2 56 -9.60247220822005 3 1 1.5320681038664006 3 2 2.05021844926533 3 3 2.963297331124444 3 4 3.723311736745785 3 5 2.068723765883064 3 6 2.41216011785324 3 7 1.613958351501637 3 8 1.9826226295570208 3 9 1.904672716416684 3 10 2.5644775697354802 3 11 1.353087801138095 3 12 1.6836218680080322 3 13 -4.346643546565996 3 14 -7.331222488671731 3 15 -2.242199589804187 3 16 2.7596622007138265 3 17 2.804917041446906 3 18 2.0857579717273573 3 19 0.9747820450114162 3 20 1.1656492124816502 3 21 3.0505277812298957 3 22 3.6812508122215633 3 23 3.1210217848527293 3 24 3.1263013486341436 3 25 2.6182621540470805 3 26 2.552519312733054 3 27 3.1365156811242727 3 28 2.9073198382597525 3 29 2.413101817452656 3 30 1.599587489132034 3 31 2.858762447238327 3 32 0.7235669884572005 3 33 2.8888164854535034 3 34 3.024138881097592 3 35 2.1293416791701176 3 36 5.352410510798432 3 37 5.11925999272734 3 38 6.215482695728369 3 39 8.044742380649968 3 40 10.491890367323396 3 41 9.223630809280088 3 42 7.047201244288313 3 43 1.9215307727850188 3 44 3.333950951447129 3 45 6.844670559658777 3 46 0.06999852174234533 3 47 -4.130887381179619 3 48 0.5131585681962569 3 49 -7.887725368626276 3 50 0.5747441740667476 3 51 -1.0275712892829496 3 52 -0.5515106170885637 3 53 -1.6045889427228701 3 54 -3.633517157553179 3 55 -1.8258928105761356 3 56 1.185481136048221 4 1 1.9303731886121653 4 2 2.558479380756573 4 3 3.2708033843932114 4 4 4.165698861087044 4 5 2.325453576451359 4 6 2.6366295666497095 4 7 1.8988892730227889 4 8 2.2155666546246735 4 9 2.4316631736165135 4 10 2.497464323759118 4 11 3.951720474546441 4 12 3.703633262396295 4 13 5.262244076279753 4 14 4.299955406215034 4 15 6.4435289388954295 4 16 6.885285331322002 4 17 6.43944203788616 4 18 6.6482134623982 4 19 5.134374695630989 4 20 5.089591670820912 4 21 3.80754245150478 4 22 4.066808425097221 4 23 3.6770679321251976 4 24 4.039205493342877 4 25 3.7085626810375416 4 26 3.026312219726364 4 27 3.5386663719090663 4 28 3.0263199806895997 4 29 2.991153377059547 4 30 3.0186873324591943 4 31 3.7020896086113524 4 32 4.442695317755025 4 33 3.4447202028726736 4 34 4.061455502661474 4 35 3.5178903189650157 4 36 6.044121093336532 4 37 6.201299588226558 4 38 6.808703034711421 4 39 8.790932382074264 4 40 10.505563633239934 4 41 6.594589362192778 4 42 9.828001700063844 4 43 8.403798256049512 4 44 12.878958785089058 4 45 9.397512035232058 4 46 8.178672499808147 4 47 8.993311450895582 4 48 8.885256559935325 4 49 4.753565045844596 4 50 8.687762262658119 4 51 7.2200430106743205 4 52 7.181718267685682 4 53 6.686395247563414 4 54 1.2351625177067547 4 55 6.143912060524848 4 56 5.393642366642151 5 1 1.7494208076597815 5 2 2.3142216776753344 5 3 2.73747005105988 5 4 3.7102366762130927 5 5 2.0576664616054217 5 6 2.6002150008233755 5 7 1.7778808696614448 5 8 1.9601044697507248 5 9 1.9593942660534864 5 10 2.0851393937871308 5 11 3.6665664129217888 5 12 6.284025419259045 5 13 6.463364524459028 5 14 6.617042240948926 5 15 6.978542944497672 5 16 6.737386171658137 5 17 6.732439236765707 5 18 5.9798661234626245 5 19 4.813926516359281 5 20 4.886230326283101 5 21 3.163844972513189 5 22 3.5245115063297163 5 23 3.1952752150383645 5 24 3.4761802832588415 5 25 2.135453437340061 5 26 2.1053038163650157 5 27 2.2972097892760104 5 28 2.315955835031339 5 29 2.0107612201968053 5 30 2.243897416492807 5 31 2.4623136982472076 5 32 3.995076270135975 5 33 1.936596953572952 5 34 2.141007323389765 5 35 1.90948695762048 5 36 3.1707317375942345 5 37 3.3486385238007905 5 38 3.618226844235231 5 39 5.784209692998633 5 40 5.099961392343577 5 41 0.5867462249378761 5 42 6.714836433957402 5 43 10.606599376497691 5 44 10.008930773884579 5 45 7.461377581450545 5 46 8.26886857823952 5 47 8.220202207198106 5 48 8.15472434705017 5 49 8.740119667693335 5 50 8.481039573582486 5 51 7.8385304056323015 5 52 7.84558381390417 5 53 8.355863034678258 5 54 8.244126103140932 5 55 8.599934469488431 5 56 7.347143767202375 6 1 2.132393390632366 6 2 2.7049864684401257 6 3 3.4858105994004234 6 4 3.922935088911508 6 5 2.569066173005133 6 6 2.487660888269268 6 7 2.470232962013533 6 8 2.440335349981602 6 9 2.566032072691293 6 10 3.084850793498532 6 11 5.166133512488891 6 12 7.681139416373043 6 13 7.191791652886156 6 14 7.73854296244965 6 15 7.551414517369242 6 16 7.587602621874581 6 17 7.182078486404961 6 18 7.017961361557862 6 19 5.037014539447306 6 20 5.932983573036349 6 21 4.084768493436705 6 22 4.26967745149566 6 23 3.967280987044134 6 24 4.438662245740801 6 25 3.5761460780327 6 26 3.388420699481898 6 27 2.921740813807034 6 28 3.381468100543607 6 29 3.7668939763295626 6 30 4.219943592538982 6 31 3.9419673779008875 6 32 5.168525043584751 6 33 4.091863908839912 6 34 4.767847150229592 6 35 4.396932845066367 6 36 4.171020337882833 6 37 4.346618321780586 6 38 5.626884852893241 6 39 6.730241439030376 6 40 2.149312041694227 6 41 4.155577393769047 6 42 8.1139706330916 6 43 13.351765321663638 6 44 11.836347801301606 6 45 8.986341506414469 6 46 11.083009992380937 6 47 11.870995857991755 6 48 11.358476150801977 6 49 12.233914761488432 6 50 11.488543181086094 6 51 11.095096062197957 6 52 12.460302428622786 6 53 11.547782226597452 6 54 11.456535915550742 6 55 11.71219998175394 6 56 10.694041314099923 7 1 1.9609854335101247 7 2 2.400616235498468 7 3 3.066721751740147 7 4 3.918399941519219 7 5 2.2420202031020224 7 6 2.481167381775755 7 7 2.020738012518585 7 8 2.2743901840364344 7 9 2.0859248782983855 7 10 2.7735747679367933 7 11 4.294457569384377 7 12 6.769059432864488 7 13 6.957922347588276 7 14 7.738130676323081 7 15 7.298270835654133 7 16 7.004733110433641 7 17 6.976656923840537 7 18 6.191430749312968 7 19 5.956103387107582 7 20 5.55357726505861 7 21 3.818947013329513 7 22 4.297300621975978 7 23 3.484390861296866 7 24 4.088425181218021 7 25 3.7415758863196515 7 26 2.2808140204466483 7 27 2.7271417620651306 7 28 2.854051073126577 7 29 2.4971557780199305 7 30 2.962264763431584 7 31 2.612653834301632 7 32 4.463103481020326 7 33 3.031154776702209 7 34 3.658694398219694 7 35 3.264588998436807 7 36 1.3292351389547754 7 37 1.0758494081545287 7 38 1.6760499734869327 7 39 2.7801280603455716 7 40 -1.8605828253434993 7 41 -9.336383026762803 7 42 4.125720787698896 7 43 9.015442913912658 7 44 8.087842338510427 7 45 4.091989826348502 7 46 6.563426401368773 7 47 7.352855268422594 7 48 7.0479216259617345 7 49 9.395221708509663 7 50 7.105529369500854 7 51 8.989550813795567 7 52 9.335379732271516 7 53 8.869468476855129 7 54 8.606711137154537 7 55 8.346192972889792 7 56 7.875034923664963 8 1 1.819262077501051 8 2 2.4120571755108315 8 3 2.475709589299413 8 4 3.7512179171943316 8 5 2.043813647752607 8 6 2.598771999380375 8 7 1.8035662953468687 8 8 2.1070020166482664 8 9 2.091284597943819 8 10 2.791921500569238 8 11 4.347951694307071 8 12 6.2063919416255615 8 13 6.616034077128578 8 14 6.9203611442678294 8 15 6.682727648682377 8 16 7.163360197632159 8 17 6.576017880344352 8 18 5.8361431797396826 8 19 5.370347872780634 8 20 5.5491451891979615 8 21 3.6908290994973143 8 22 4.0676572494754595 8 23 3.5228365425996913 8 24 3.41846022553878 8 25 3.4347319366185616 8 26 1.883370194431393 8 27 2.4873973794636015 8 28 2.118841837917344 8 29 2.484065693501279 8 30 3.5128728854682763 8 31 3.4975229334564446 8 32 3.946302821362522 8 33 2.0514598684358702 8 34 2.404210786593225 8 35 2.3363267844603044 8 36 -0.35423218736969275 8 37 -0.5220685469062829 8 38 0.7682989943073792 8 39 2.336302045090985 8 40 -2.4971526047704202 8 41 -9.511089272897621 8 42 4.0129605320815 8 43 8.59418956408788 8 44 7.755539720493523 8 45 4.875230395303358 8 46 7.386040295411238 8 47 7.7194807064766024 8 48 8.550395342721167 8 49 10.557147084720754 8 50 7.882482575025488 8 51 9.579944547046441 8 52 9.803736772057132 8 53 10.244751923567147 8 54 10.678758137772963 8 55 9.025331294885255 8 56 7.582930202988813 9 1 1.88135358076903 9 2 2.2881712575072726 9 3 2.7133804152055347 9 4 3.9163991411990864 9 5 2.237218282333714 9 6 2.4377500148289784 9 7 2.0403458556558434 9 8 2.1049223969215873 9 9 2.018497907510067 9 10 2.5506856122745276 9 11 4.178611230848958 9 12 6.31987976099574 9 13 6.238434552470228 9 14 7.161299944030162 9 15 6.947730619567697 9 16 6.963716703871022 9 17 6.117313186345541 9 18 5.468941753714727 9 19 4.907483939328465 9 20 4.938331166619232 9 21 3.017626485118228 9 22 3.542998901287696 9 23 3.1140427220411624 9 24 3.2913063336790103 9 25 3.1679464345389405 9 26 2.7672085782697806 9 27 2.0316635707886164 9 28 2.225199532510331 9 29 2.730649175378879 9 30 2.5018806097701187 9 31 3.33234170945169 9 32 4.760622488623369 9 33 2.884496113236821 9 34 3.445209004062029 9 35 2.85682589319471 9 36 2.4110678720480117 9 37 2.176089504192948 9 38 3.416546171966324 9 39 5.106058432494432 9 40 -2.2143243219421365 9 41 -2.3773994333254294 9 42 6.33500450118429 9 43 11.017243634200774 9 44 9.376157664640882 9 45 7.495551250918332 9 46 9.688476421376777 9 47 10.35465598871071 9 48 9.910466643968938 9 49 11.906226110270367 9 50 10.043504559576885 9 51 8.271863738965635 9 52 8.281158043596047 9 53 8.218608132717474 9 54 8.357011257202554 9 55 8.306377046519245 9 56 7.2017656159418735 10 1 1.973383941300341 10 2 2.2859574687659645 10 3 3.040388637849432 10 4 3.9802827591623995 10 5 2.383012083725241 10 6 2.4466051697941893 10 7 2.1658993028411686 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2.7116900839005993 116 4 2.9018622919766415 116 5 1.964398809717082 116 6 2.1412659039432427 116 7 1.3828069780358234 116 8 1.6857202332975234 116 9 1.5992957438860032 116 10 2.003694401997315 116 11 -0.582037856372132 116 12 -1.4177808204782316 116 13 -6.682119876197788 116 14 -10.51415644542217 116 15 -4.278435709032706 116 16 -8.06704732587881 116 17 -9.548677347799153 116 18 -3.37859036258007 116 19 -3.6198994278114895 116 20 -4.178301693421332 116 21 1.8594114749762412 116 22 1.9936412271835735 116 23 1.8422374482074915 116 24 2.106722155180023 116 25 2.3812662452218305 116 26 2.5545649001088577 116 27 2.6821030569279003 116 28 2.1717850632743634 116 29 2.5805477555005822 116 30 3.403503327822854 116 31 1.5077980989040256 116 32 -6.251885963033157 116 33 1.5586002376452015 116 34 1.2061961575441131 116 35 1.2459401595909227 116 36 3.5970043155909472 116 37 4.416454615754812 116 38 5.308703034711421 116 39 6.875835308762181 116 40 9.205215907942918 116 41 6.419422743821293 116 42 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1.3547937554021345 118 7 0.6938149355955048 118 8 0.39526930491555845 118 9 1.1357678924271113 118 10 1.6091320677798038 118 11 -8.698085601730227 118 12 -11.81963758440396 118 13 -9.77363181253731 118 14 -3.0672068433001556 118 15 -17.52512005916533 118 16 -10.002723718451755 118 17 -13.47506992074345 118 18 -6.020500176903678 118 19 -4.649077146644384 118 20 -5.673659783607011 118 21 -0.36472645605824283 118 22 -0.6873932555750457 118 23 -0.6902559204927741 118 24 -2.776566969488414 118 25 -0.09751359562697104 118 26 1.1646444757056784 118 27 1.5886017306679534 118 28 0.6260290951045988 118 29 0.25892971836530165 118 30 1.0301611527565413 118 31 -2.554536118602609 118 32 -10.257854132794428 118 33 -1.2663334493574467 118 34 -0.4197985374160993 118 35 -1.0564471083135878 118 36 1.7813544482169412 118 37 1.5550487802110453 118 38 2.481779957788344 118 39 3.918275627064567 118 40 6.099778242160426 118 41 2.8484678366594878 118 42 -0.8260060568850918 118 43 0.02289974279805662 118 44 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0.752057063118265 119 27 1.3106297026959268 119 28 1.4092458783213857 119 29 1.451237410672995 119 30 1.677014299609688 119 31 -2.3080326220991125 119 32 -13.450161825102121 119 33 -1.5040956871196869 119 34 -1.4897286073461657 119 35 -1.0879156397821177 119 36 1.6432425601050547 119 37 2.268335493497755 119 38 3.2405212165296007 119 39 3.7049889137778536 119 40 6.732645375027559 119 41 6.367698605890258 119 42 2.6670009361219016 119 43 -1.0977296278313133 119 44 -0.49193502698122415 119 45 -0.6853756653027006 119 46 -7.565979906608964 119 47 -8.737590000594103 119 48 -9.888998596672772 119 49 -8.619620592046923 119 50 -9.407921465378552 119 51 -10.061469594367697 119 52 -10.138182419862064 119 53 -11.43201575320053 119 54 -10.692453983439156 119 55 -10.941840422286461 119 56 -6.566059696001087 120 1 0.4405772702448161 120 2 0.7577590926413222 120 3 1.6789666496993334 120 4 2.5306448394783985 120 5 0.9052855092244698 120 6 1.0015755450410637 120 7 0.6227788288451137 120 8 0.7437779391384751 120 9 0.26959834768614144 120 10 1.5286768087531186 120 11 -11.899419981636033 120 12 -3.261552812033475 120 13 -6.521669489146415 120 14 -4.8741142216361055 120 15 -4.273157735774436 120 16 -11.964654644668396 120 17 -10.21722062717987 120 18 -5.431018230278873 120 19 -4.094917021055686 120 20 -5.477034979839349 120 21 -1.4157468642215107 120 22 -1.3557606025138185 120 23 -2.1380581182949676 120 24 -5.9319829820472805 120 25 -2.227811868782389 120 26 1.4440793265690957 120 27 0.523060129412066 120 28 0.578540869044943 120 29 0.9359312882240189 120 30 1.003081089962194 120 31 -3.8873461656983714 120 32 -11.618529172040898 120 33 -3.95864114166514 120 34 -10.780081111984387 120 35 -3.490513042379522 120 36 -1.558519963086038 120 37 -2.4241505918454713 120 38 -4.232113291819193 120 39 -2.321912755980957 120 40 -4.63609302942513 120 41 -0.38740343492606755 120 42 -5.384483293933755 120 43 -2.1172101473118303 120 44 -3.136647457407939 120 45 -7.8773979287535365 120 46 -11.140655231284288 120 47 -11.106328405046794 120 48 -10.411262047507652 120 49 -13.533349720061766 120 50 -10.588348181519553 120 51 -11.296163471918717 120 52 -11.0523753697693 120 53 -11.957062135389767 120 54 -10.546350087335261 120 55 -11.541562129151025 120 56 -8.06374058653912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 482.0 5 34.0 6 76.0 7 84.0 8 190.0 9 270.0 10 172.0 11 121.0 12 126.0 13 150.0 14 205.0 15 279.0 16 441.0 17 589.0 18 711.0 19 910.0 20 1055.0 21 1271.0 22 1630.0 23 1859.0 24 2127.0 25 2252.0 26 2390.0 27 2500.0 28 2474.0 29 2636.0 30 2889.0 31 3449.0 32 4142.0 33 5041.0 34 5738.0 35 6855.0 36 7388.0 37 5992.0 38 3028.0 39 797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.26655579719414 0.6396315722144045 0.045484911801913205 0.048327718789532785 8 99.61053544269612 0.2984947337000554 0.03979929782667406 0.051170525777152365 9 95.68975321278289 2.482088024564995 1.0064824292050494 0.8216763334470603 10 50.82938893863801 30.641195116057595 9.772149019942292 8.757266925362101 11 38.72866057341047 24.507114326733095 18.82560304757573 17.938622052280706 12 36.60824698307108 13.069805125580999 20.969965744175802 29.351982147172116 13 26.97681690901596 13.084019160519098 24.151066763322106 35.78809716714284 14 22.167725792923047 14.870416968766436 29.850300678125137 33.111556560185385 15 26.899851885610115 15.855360601572292 27.969408681781932 29.27537883103566 16 33.719640521017006 18.07206643535635 23.405949604686878 24.802343438939765 17 31.16772340848995 20.506951750021322 23.171078445310055 25.15424639617867 18 31.32382834155425 22.480774424654225 23.410425165958294 22.78497206783323 19 30.918835923172068 24.65204225251994 20.395513157707672 24.03360866660032 20 28.519429554534987 26.200395273776856 22.315906214898124 22.964268956790036 21 27.236800955278195 21.691354163705114 27.489835953714497 23.58200892730219 22 26.590951088115304 20.417620218618072 27.860300493241034 25.131128200025586 23 25.98325586684292 22.526402569897517 26.912853751794525 24.57748781146504 24 26.445212002331104 24.52347447870027 24.441033076059302 24.59028044290933 25 29.554241528314762 23.87849670229702 22.48123720718672 24.0860245622015 26 22.85787510322636 21.258934419227156 27.37420622490532 28.508984252641174 27 23.373428880168344 17.940624466814537 29.659330034692598 29.026616618324518 28 22.45191417873261 23.310411594698103 26.920941357365567 27.31673286920372 29 26.91870219239628 22.986038047032732 27.41206244490574 22.683197315665254 30 25.46173204612344 19.158858572789445 32.7015767847241 22.677832596363015 31 23.843776261626335 20.962297206831227 33.421168829319015 21.772757702223426 32 30.767262336333008 17.029897214995522 29.56454983579989 22.638290612871582 33 29.230396764362414 18.943860548577288 31.239853029137826 20.585889657922472 34 21.91509943949697 21.21375935357214 37.15110820269155 19.720033004239337 35 22.27594641485822 24.529992320600698 36.27236269518473 16.92169856935635 36 24.74270828884998 24.637371710011248 30.929097094703277 19.690822906435496 37 24.538330008549444 24.006839555428897 32.15303505272157 19.301795383300085 38 26.56863861527074 23.98792916927632 31.152138017422992 18.291294198029952 39 22.73609406661149 18.43945318074146 27.550729187476815 31.273723565170236 40 15.878281759423441 14.165189040782474 23.93753932391466 46.01898987587943 41 16.078161512766474 16.78064025920032 26.46337791922928 40.67782030880392 42 20.764902784701945 21.371697172566055 35.226835695463286 22.636564347268713 43 13.577921429284928 12.316451902906802 26.96034362201578 47.14528304579248 44 15.518596310743424 13.691114064040462 23.67868318401989 47.11160644119622 45 22.60754486075733 20.020491511675893 33.83945469810596 23.532508929460818 46 21.182294037323622 21.724226217569413 32.756315430132 24.337164314974967 47 18.17393159354334 20.881746426793715 33.21481902865676 27.729502951006186 48 21.347307943911943 23.46340908444495 34.77061349867744 20.41866947296567 49 16.40653978942563 15.074043427397497 23.321539646760066 45.197877136416814 50 21.25368542494552 22.025666225127832 33.48858408466151 23.232064265265137 51 21.097894916994694 24.71475840036511 33.43174168520737 20.755604997432826 52 20.364399041205342 24.19101700719096 34.636457025453716 20.808126926149985 53 22.732322944561563 21.34066434960971 35.0863198678138 20.84069283801493 54 17.88301945879774 20.076582539750323 31.330514868115756 30.70988313333618 55 23.011873238233534 22.483699211298084 32.64286324421287 21.861564306255516 56 22.017120312210686 21.985785298181145 32.15684598840605 23.840248401202125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.5 14 1.0 15 8.5 16 16.0 17 32.5 18 49.0 19 66.5 20 84.0 21 120.5 22 214.0 23 271.0 24 323.0 25 375.0 26 426.0 27 477.0 28 607.0 29 867.5 30 998.0 31 1185.0 32 1372.0 33 1555.5 34 1739.0 35 1799.5 36 1860.0 37 1818.5 38 1612.5 39 1448.0 40 1396.0 41 1344.0 42 1264.5 43 1185.0 44 1240.0 45 1295.0 46 1340.0 47 1485.0 48 1585.0 49 1734.0 50 1883.0 51 2220.5 52 2558.0 53 2569.0 54 2602.5 55 2625.0 56 2777.0 57 2929.0 58 3218.0 59 3507.0 60 3674.0 61 3841.0 62 4230.0 63 5015.0 64 5411.0 65 5323.0 66 5235.0 67 4807.0 68 4379.0 69 4007.5 70 3636.0 71 3224.5 72 2523.0 73 2233.0 74 1955.0 75 1677.0 76 1343.0 77 1009.0 78 796.0 79 461.5 80 340.0 81 257.5 82 175.0 83 130.5 84 86.0 85 69.5 86 53.0 87 34.0 88 9.5 89 4.0 90 3.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.012792631444288091 10 0.0 11 0.0028428069876195753 12 0.0 13 0.0 14 0.017056841925717454 15 0.19473227865194093 16 0.041220701320483846 17 0.015635438431907667 18 0.005685613975239151 19 0.01989964891333703 20 0.031270876863815335 21 0.009949824456668515 22 0.0028428069876195753 23 0.0 24 0.0 25 0.0014214034938097877 26 0.16914701576336474 27 0.02984947337000554 28 0.1620399982943158 29 0.027006666382385965 30 0.028428069876195755 31 0.207524910096229 32 0.017056841925717454 33 0.19188947166432135 34 0.08386280613477748 35 0.04974912228334257 36 0.14498315636859835 37 0.24590280442909326 38 0.14356175287478856 39 0.3908859607976916 40 0.5984108708939206 41 0.8528420962858727 42 0.2103677170838486 43 0.3923073642915015 44 0.5202336787343823 45 0.11371227950478302 46 0.0696487711966796 47 0.05401333276477193 48 0.04974912228334257 49 0.36814350489673503 50 0.20468210310860946 51 0.33687262803291973 52 0.37667192585959375 53 0.2117891205776584 54 0.14498315636859835 55 0.15777578781288645 56 0.20468210310860946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 70353.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.64164996517562 #Duplication Level Percentage of deduplicated Percentage of total 1 93.47879583299155 80.9915710772817 2 4.395045525387581 7.615879919832843 3 0.9695677138872939 2.5201483945247536 4 0.45279304404888854 1.569229457166006 5 0.20999097695020918 0.9096982360382642 6 0.11647937002706917 0.6055178883629696 7 0.08038717086375195 0.4875413983767572 8 0.05905996226724633 0.4093642062172189 9 0.04265441719301124 0.33260841755149034 >10 0.17061766877204496 2.4334427814023565 >50 0.0180460995816586 1.0887950762582974 >100 0.006562218029694037 1.0362031469873352 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 281 0.3994143817605504 No Hit CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 206 0.29280911972481627 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 123 0.1748326297386039 No Hit CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 119 0.16914701576336474 No Hit CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 99 0.140718945887169 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 89 0.1265049109490711 No Hit CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 81 0.1151336829985928 No Hit CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 75 0.10660526203573409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0014214034938097879 0.0 0.0 0.0 0.0 11 0.0042642104814293634 0.0 0.0 0.0 0.0 12 0.0056856139752391515 0.0 0.0 0.0 0.0 13 0.007107017469048939 0.0 0.0 0.0 0.0 14 0.007107017469048939 0.0 0.0 0.0 0.0 15 0.011371227950478303 0.0 0.0 0.0 0.0 16 0.014214034938097878 0.0 0.0 0.0 0.0 17 0.015635438431907664 0.0 0.0 0.0 0.0 18 0.01847824541952724 0.0 0.0 0.0 0.0 19 0.01847824541952724 0.0 0.0 0.0 0.0 20 0.01989964891333703 0.0 0.0 0.0 0.0 21 0.01989964891333703 0.0 0.0 0.0 0.0 22 0.021321052407146816 0.0 0.0 0.0 0.0 23 0.021321052407146816 0.0 0.0 0.0 0.0 24 0.024163859394766393 0.0 0.0 0.0 0.0 25 0.02558526288857618 0.0 0.0 0.0 0.0 26 0.02558526288857618 0.0 0.0 0.0 0.0 27 0.029849473370005545 0.0 0.0 0.0 0.0 28 0.03269228035762512 0.0 0.0 0.0 0.0 29 0.03411368385143491 0.0 0.0 0.0 0.0 30 0.035535087345244694 0.0 0.0 0.0 0.0 31 0.035535087345244694 0.0 0.0 0.0 0.0 32 0.035535087345244694 0.0 0.0 0.0 0.0 33 0.035535087345244694 0.0 0.0 0.0 0.0 34 0.035535087345244694 0.0 0.0 0.0 0.0 35 0.035535087345244694 0.0 0.0 0.0 0.0 36 0.035535087345244694 0.0 0.0 0.0 0.0 37 0.03695649083905448 0.0 0.0 0.0 0.0 38 0.038377894332864274 0.0 0.0 0.0 0.0 39 0.038377894332864274 0.0 0.0 0.0 0.0 40 0.038377894332864274 0.0 0.0 0.0 0.0 41 0.038377894332864274 0.0 0.0 0.0 0.0 42 0.041220701320483846 0.0 0.0 0.0 0.0 43 0.041220701320483846 0.0 0.0 0.0 0.0 44 0.041220701320483846 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGCT 15 0.008167848 50.35273 41 CGATTAA 35 5.0549716E-8 50.172516 49 AAGTGCC 15 0.008284548 50.172512 34 TACCAAG 25 2.0341276E-5 50.136627 47 CAACGAT 40 2.557499E-9 50.136627 46 AACGATT 40 2.557499E-9 50.136627 47 CCGACCG 25 2.0341276E-5 50.136627 44 CTACACG 15 0.008308036 50.136623 46 CGACTCC 15 0.008308036 50.136623 44 CTCTCAC 15 0.008308036 50.136623 47 GCCCGCG 15 0.008308036 50.136623 46 TCGCCTA 25 2.0427646E-5 50.10079 42 ACCTATT 15 0.008331574 50.10079 42 TTAGTTG 15 0.008331574 50.10079 42 TGTAGCG 15 0.008331574 50.10079 42 CACAATT 15 0.008331574 50.10079 42 CGCCTAG 20 4.1302538E-4 50.065 43 CCTATTC 15 0.008355161 50.065 43 ATGATTT 15 0.008378797 50.029266 33 CATGATT 15 0.0084024845 49.993584 32 >>END_MODULE