FastQCFastQC Report
Fri 17 Jun 2016
SRR1526291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83493
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2970.3557184434623262No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2570.30781023558861226No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2400.2874492472422838No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1420.17007413795168458No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1280.1533062651958847No Hit
GATCTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1280.1533062651958847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAA150.0080789350.50060738
AACCATA203.9615243E-450.50060338
TTCGTTT203.9615243E-450.50060338
ACCAACC402.408342E-950.50060338
CAAACCA800.050.46994439
AGATCCA150.00809836650.46994439
GCGCCCT150.00809836650.46994439
TCGTCTC203.9734284E-450.46994440
ACTAAAA150.00809836650.46994439
CGTCTCA402.4338078E-950.4393241
AGCCATC150.00813734150.40873337
TGCCCGT203.9973218E-450.4087337
TTTCGTT203.9973218E-450.4087337
AAACCAT203.9973218E-450.4087337
ATGCTCG204.009311E-450.3781836
TATGCCT309.835258E-750.3781835
GTTGTCG150.00815688150.3781836
TGATAAC150.00825510450.22598334
CCGATTC204.0940396E-450.1653643
ATAGAAT150.00829463850.1653642