##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526291_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83493 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.87316301965434 39.0 38.0 40.0 35.0 40.0 2 36.704082977016036 39.0 37.0 40.0 32.0 40.0 3 37.17148743008396 39.0 38.0 40.0 33.0 40.0 4 37.05157318577605 39.0 38.0 40.0 33.0 40.0 5 37.499239457200005 39.0 38.0 40.0 35.0 40.0 6 37.53600900674308 39.0 38.0 40.0 35.0 40.0 7 37.40118333273448 39.0 38.0 40.0 34.0 40.0 8 37.20206484375936 39.0 38.0 40.0 33.0 40.0 9 37.16371432335645 39.0 38.0 40.0 33.0 40.0 10 36.859377432838684 39.0 37.0 40.0 33.0 40.0 11 35.33503407471285 38.0 34.0 39.0 28.0 40.0 12 33.23005521420957 38.0 31.0 39.0 18.0 40.0 13 32.6959026505216 37.0 30.0 39.0 16.0 40.0 14 32.335477225635685 37.0 29.0 39.0 15.0 40.0 15 32.74239756626304 37.0 30.0 39.0 17.0 40.0 16 32.27986777334627 36.0 29.0 39.0 17.0 40.0 17 32.359311559052856 36.0 29.0 39.0 17.0 40.0 18 32.501646844645656 36.0 29.0 39.0 18.0 40.0 19 33.414801240822584 36.0 31.0 39.0 23.0 40.0 20 33.5572083887272 36.0 31.0 39.0 23.0 40.0 21 35.03921286814464 38.0 35.0 39.0 26.0 40.0 22 34.72232402716396 38.0 34.0 39.0 25.0 40.0 23 34.985807193417415 38.0 34.0 39.0 26.0 40.0 24 34.53448792114309 38.0 33.0 39.0 24.0 40.0 25 34.89458996562586 38.0 34.0 39.0 26.0 40.0 26 35.271831171475455 38.0 35.0 39.0 27.0 40.0 27 35.28844334255567 38.0 35.0 39.0 27.0 40.0 28 35.17209825973435 38.0 35.0 39.0 27.0 40.0 29 35.03079300061083 38.0 35.0 39.0 27.0 40.0 30 34.79591103445798 38.0 35.0 39.0 26.0 40.0 31 34.53083492029272 38.0 34.0 39.0 25.0 40.0 32 33.478291593307226 38.0 33.0 39.0 18.0 40.0 33 34.6555759165439 38.0 35.0 39.0 26.0 40.0 34 34.44255207023343 38.0 34.0 39.0 25.0 40.0 35 34.380019881906264 38.0 34.0 39.0 25.0 40.0 36 32.103074509240294 35.0 31.0 38.0 19.0 39.0 37 31.845412190243493 35.0 31.0 38.0 17.0 39.0 38 31.144934305869953 35.0 30.0 38.0 10.0 39.0 39 29.19168073970273 34.0 27.0 38.0 4.0 39.0 40 25.148707077240008 31.0 11.0 38.0 4.0 39.0 41 21.60666163630484 25.0 8.0 33.0 4.0 37.0 42 25.30164205382487 30.0 20.0 34.0 4.0 37.0 43 21.139065550405423 25.0 7.0 33.0 4.0 36.0 44 22.4364557507815 27.0 8.0 33.0 4.0 37.0 45 24.976788473285186 30.0 18.0 34.0 4.0 37.0 46 24.96052363671206 30.0 10.0 36.0 4.0 38.0 47 24.154300360509264 30.0 5.0 35.0 4.0 38.0 48 24.100487466015114 29.0 4.0 35.0 4.0 38.0 49 21.441330410932654 26.0 4.0 35.0 4.0 38.0 50 23.813289736864167 29.0 4.0 35.0 4.0 38.0 51 25.33147689027823 32.0 4.0 38.0 4.0 40.0 52 25.15074317607464 32.0 4.0 38.0 4.0 40.0 53 24.716838537362413 31.0 4.0 38.0 4.0 40.0 54 23.843112596265556 30.0 4.0 37.0 4.0 40.0 55 24.012935216125904 31.0 4.0 37.0 4.0 40.0 56 23.58243206017271 31.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6595103571736018 1 2 1.7060944264407283 1 3 1.6666844524855264 1 4 1.3906209762447332 1 5 1.0324034256786163 1 6 1.137467905299836 1 7 1.3827630930618042 1 8 1.4091718622016032 1 9 1.56338514147307 1 10 1.7378815108616266 1 11 -3.479873962232624 1 12 -7.416440078614915 1 13 -21.17999564200784 1 14 -17.948447144378292 1 15 -11.203022869517401 1 16 -21.45977664151619 1 17 -21.16736555931319 1 18 -20.836784842344443 1 19 -5.842170330264498 1 20 -10.367240383496753 1 21 -8.951566126550567 1 22 -3.525517392831965 1 23 -3.8063143248888416 1 24 -4.127389377385327 1 25 -4.186200177322771 1 26 2.5252189396129907 1 27 2.602895307620777 1 28 2.4281725752754255 1 29 2.289272311565739 1 30 2.737244799153771 1 31 1.862915153258335 1 32 -11.889211652571227 1 33 0.49998034231042254 1 34 0.618923570910205 1 35 0.3963057111430004 1 36 3.1054363034814045 1 37 3.2084239594097603 1 38 4.0081725176026985 1 39 6.367717859656057 1 40 7.5244306427878875 1 41 -5.429985590257541 1 42 -10.763647518869153 1 43 -12.708052279873204 1 44 -16.059785214538604 1 45 -14.552457286072721 1 46 -18.55566188949136 1 47 -18.169516649700643 1 48 -16.77865791834834 1 49 -14.456868992643898 1 50 -16.458184845626576 1 51 -17.822788430058527 1 52 -17.618649177705997 1 53 -17.441522301602884 1 54 -17.791997972938375 1 55 -16.866420677415633 1 56 -16.01231969355167 2 1 0.48362909557805267 2 2 1.0749255952718997 2 3 1.4232707233575113 2 4 0.9767063194729317 2 5 0.6687670620422494 2 6 0.6992489813666225 2 7 1.0410191227464054 2 8 1.1498026599752578 2 9 1.1361124142003405 2 10 1.1425197297855618 2 11 -2.9038071718801177 2 12 -11.02664416024757 2 13 -12.141591189317673 2 14 -14.896684621187195 2 15 -9.019534928886603 2 16 -5.037327661924351 2 17 -3.418942553747325 2 18 -11.27667352509027 2 19 -2.975379977759861 2 20 -3.876145763830703 2 21 2.0239440775310698 2 22 2.6930354828637704 2 23 1.901106825389455 2 24 2.3611078396833207 2 25 2.280590175181864 2 26 2.2129740416538013 2 27 2.400854491294247 2 28 2.70646571071142 2 29 2.023595131602846 2 30 2.1570963761482034 2 31 -2.1066581306006604 2 32 -10.45711517761761 2 33 -2.540279397949316 2 34 -1.6285717908708683 2 35 -1.8686293537920662 2 36 3.1698147821456004 2 37 3.5097226607084657 2 38 4.171808881239063 2 39 6.351762386557727 2 40 8.66079427915152 2 41 5.494689734417783 2 42 1.3554619430974526 2 43 -2.804156175977102 2 44 -10.31711360043842 2 45 -0.6593218500801434 2 46 -12.256032947005277 2 47 -11.917568597752592 2 48 -9.661218215194351 2 49 -11.99137734143796 2 50 -9.891580021878896 2 51 -9.945793995921235 2 52 -9.960949734292269 2 53 -10.117403563198431 2 54 -14.774187212270473 2 55 -9.403897486321014 2 56 -9.29079835774462 3 1 0.7791495171854308 3 2 1.2826989813061545 3 3 1.4281455586671257 3 4 1.0275627094597581 3 5 0.9961715416206474 3 6 0.9755335663468614 3 7 1.1703998869097774 3 8 1.4156788128079327 3 9 1.4474431124875622 3 10 1.2622825756353677 3 11 0.31885422469432 3 12 0.22552975279590726 3 13 -6.570708448869716 3 14 -7.934102275996153 3 15 -4.6840935586626244 3 16 1.7786143670611558 3 17 1.8888308322869314 3 18 1.663510928137665 3 19 0.4367412343613495 3 20 0.23953276054347583 3 21 2.6137991499948328 3 22 3.1445506343789233 3 23 2.4791041903433353 3 24 2.1439800399468254 3 25 2.7738708075929353 3 26 1.811524766291484 3 27 2.1023037666565685 3 28 1.6675987805401462 3 29 1.7596952633551552 3 30 1.7126958491389814 3 31 2.5548701961450604 3 32 0.843411831605053 3 33 2.1200631580454115 3 34 2.379201595163387 3 35 1.4822270361947574 3 36 4.590499894135057 3 37 4.839893938705831 3 38 5.711071068327339 3 39 7.714212979443108 3 40 10.06790890365745 3 41 9.19113242216482 3 42 6.9078993607522605 3 43 1.2008504116381822 3 44 2.6357190741333874 3 45 6.612878413424468 3 46 -1.38515020655732 3 47 -6.207818927133356 3 48 -1.560822958277356 3 49 -10.707978131951794 3 50 -2.416481207649646 3 51 -4.048165537423213 3 52 -3.760027468152611 3 53 -4.140723721301196 3 54 -7.889602232033319 3 55 -4.289668237309158 3 56 -1.2135915066247271 4 1 0.9127827005937235 4 2 1.085897382105756 4 3 1.9756218205361904 4 4 1.2992768523882958 4 5 1.0944723911958576 4 6 1.346897884187939 4 7 1.4707996870097304 4 8 1.8833449797244768 4 9 1.549592038024791 4 10 1.0763755291586037 4 11 2.5783244895618864 4 12 3.1423213569938113 4 13 4.008252070870412 4 14 2.1967323067124944 4 15 4.962283252931577 4 16 6.224741303592889 4 17 5.881684405500327 4 18 4.996994186508477 4 19 4.266626291832615 4 20 4.364920066890299 4 21 3.0032544223586513 4 22 3.6560448872524844 4 23 3.1559657595587325 4 24 3.6545247675830055 4 25 3.33294127236055 4 26 2.916422317515874 4 27 2.1698200085356305 4 28 2.5826412593007646 4 29 2.714817702135761 4 30 2.7059991974648128 4 31 3.309642809838209 4 32 3.619373850595558 4 33 2.7866798496995884 4 34 2.7651586166526414 4 35 2.598768765330192 4 36 4.109940173995128 4 37 4.6956160776363625 4 38 5.373689758982007 4 39 7.098470850507571 4 40 9.324430642787885 4 41 5.195630173289256 4 42 7.404051284790242 4 43 6.922489592047977 4 44 10.123325271034936 4 45 7.667950877192585 4 46 6.621396520079362 4 47 6.825064631087532 4 48 6.106493383551729 4 49 3.9371493043300667 4 50 6.797761670911072 4 51 4.5065571012574495 4 52 4.0522164099083575 4 53 3.2496810763000035 4 54 -1.128732666815928 4 55 3.395789034055163 4 56 2.438982206518702 5 1 1.3997244756078544 5 2 1.7810358784313607 5 3 2.293315432746482 5 4 1.6032338902627998 5 5 1.8848624420720554 5 6 1.7324829605022458 5 7 1.8730608515094502 5 8 2.105391333596721 5 9 1.6945326824566749 5 10 1.3937864958064239 5 11 3.5261481187309087 5 12 5.917618134834399 5 13 5.938438616941639 5 14 5.644895110556472 5 15 6.316381613587318 5 16 6.605295288895324 5 17 6.362577565477711 5 18 5.501120811720458 5 19 5.261877846383207 5 20 4.551126963442023 5 21 2.8731244053999205 5 22 3.245643530553785 5 23 3.4365762739736567 5 24 3.9912121615909157 5 25 3.1145693108003485 5 26 2.568711746571836 5 27 2.7057725240811337 5 28 2.7414880654059033 5 29 2.736411823108064 5 30 2.781239446192913 5 31 3.0079469364634264 5 32 4.119486908820541 5 33 2.9027906467600744 5 34 2.7381942299935105 5 35 2.5333080530633723 5 36 3.631364707629949 5 37 4.341913420768076 5 38 5.106533173340406 5 39 7.371732580298417 5 40 6.049020806722314 5 41 2.704674831288127 5 42 8.557697412546037 5 43 12.028481677972227 5 44 10.484376712527304 5 45 8.492541041127012 5 46 8.550226367450758 5 47 9.366783116107317 5 48 9.310167775863771 5 49 10.071066771825826 5 50 9.41602057424629 5 51 8.55375891018905 5 52 9.100153097302364 5 53 9.429839357814984 5 54 8.977824710233254 5 55 9.3938670442304 5 56 9.059276157903664 6 1 1.2218686338175928 6 2 1.7060944264407283 6 3 2.268725268812055 6 4 1.7908477336143491 6 5 1.7228796161548061 6 6 1.4379214200390606 6 7 1.7818560635833478 6 8 2.1268589370315354 6 9 1.9919565700445006 6 10 1.3637318509976808 6 11 3.566611298538355 6 12 6.3193875857841775 6 13 6.183949936223449 6 14 6.1014394769369 6 15 6.363870554518883 6 16 6.527751703155015 6 17 6.796353261548493 6 18 5.809473661056916 6 19 4.841503139123255 6 20 5.241603153918213 6 21 3.569975823562814 6 22 3.573122062950347 6 23 3.1506017748844 6 24 3.568755747331224 6 25 3.27525106984276 6 26 2.9764661051458674 6 27 2.9135529039926595 6 28 2.1696691739148832 6 29 2.9967553047630204 6 30 3.3596937787456014 6 31 4.06246163851911 6 32 4.6550060345036 6 33 3.4047422470723276 6 34 3.112120849821771 6 35 2.5629723778096647 6 36 3.239223151553972 6 37 2.938582689568495 6 38 4.024045533475718 6 39 5.634157768953113 6 40 3.087922706279951 6 41 0.665252504980554 6 42 6.034538422173931 6 43 10.942741370920444 6 44 8.894863311538494 6 45 6.618744527986237 6 46 8.734587543615213 6 47 7.901911921727926 6 48 7.873269519293384 6 49 9.290296540235921 6 50 7.680137149838274 6 51 8.085374835247595 6 52 7.572960799618265 6 53 8.017661371866506 6 54 8.783965745882483 6 55 7.6709942885707605 6 56 7.55797509102883 7 1 1.1968381087870625 7 2 1.3049955253418304 7 3 1.760178260265043 7 4 1.701104143870758 7 5 1.348642741917935 7 6 1.440973923091569 7 7 1.5748963566236398 7 8 1.4888857990583944 7 9 1.9138124919004227 7 10 0.6457831330489654 7 11 3.191763923690978 7 12 5.697287463684056 7 13 5.276746029019542 7 14 5.707666583164009 7 15 5.767411458059783 7 16 6.188313363716677 7 17 5.5222384874337145 7 18 4.962098813682072 7 19 4.22306602068614 7 20 4.046853459168517 7 21 2.6957389493259356 7 22 3.020619010447298 7 23 1.9625675868502128 7 24 1.8953735739490511 7 25 2.1653609599526504 7 26 1.1992988279785877 7 27 1.825640816080572 7 28 0.6586801629258758 7 29 1.2513340593417723 7 30 1.7803286993805258 7 31 1.3213138973713683 7 32 2.8216727011702716 7 33 0.9664028087328873 7 34 0.5425237802247054 7 35 0.19423000906729726 7 36 -1.3749404626096435 7 37 -0.6664234154376132 7 38 -0.3166138071836251 7 39 2.1829978177931615 7 40 -2.2277061093488655 7 41 -9.945248105520056 7 42 2.7811806688161766 7 43 6.893901322080396 7 44 5.83137124804643 7 45 2.8696602789019856 7 46 5.034953843981512 7 47 5.097272117088124 7 48 5.214539360563226 7 49 7.688342938282318 7 50 5.39381236351349 7 51 6.222737472610234 7 52 6.46795469461216 7 53 6.36503621924135 7 54 6.9994724613892 7 55 5.999443617020088 7 56 5.872993406047144 8 1 1.1691141987102114 8 2 1.0128171155163628 8 3 2.4914143444423047 8 4 1.6475237373491751 8 5 1.7466891399643316 8 6 1.7269036796095563 8 7 2.006412552845717 8 8 2.1114527745665512 8 9 1.9667464860108836 8 10 1.7895021591209357 8 11 3.7272088709006326 8 12 6.16579281454235 8 13 6.077507359192637 8 14 6.6920090380946995 8 15 6.3521992193181305 8 16 6.414773178411782 8 17 6.522310311034953 8 18 5.756252372541514 8 19 4.929738433240907 8 20 4.811631165122698 8 21 2.3953726489596363 8 22 3.2523937716338196 8 23 3.1888837543428536 8 24 2.9968603224946264 8 25 3.068901863493558 8 26 2.118856394594978 8 27 1.6857284408740796 8 28 2.0758316389008797 8 29 2.23344998263417 8 30 2.321878652695183 8 31 2.3154961856713 8 32 3.4211124770806336 8 33 1.894938325503702 8 34 2.5504961999618274 8 35 1.946725879210227 8 36 -0.99420355246097 8 37 -0.6949373851280534 8 38 0.30229016466152103 8 39 2.3713193002322903 8 40 -3.9041407857835395 8 41 -10.337548615467625 8 42 3.5550799721272455 8 43 7.409594778950325 8 44 5.685712984741109 8 45 3.3923206250917453 8 46 6.447939551084868 8 47 7.162416123408601 8 48 7.337788660283113 8 49 9.352854896911925 8 50 6.483591865057697 8 51 8.182013490709782 8 52 7.6280495017657906 8 53 7.752955489513564 8 54 8.83765388780592 8 55 8.304047509859277 8 56 7.438460618573178 9 1 1.4499203264710516 9 2 0.681442087629101 9 3 2.0961588971306426 9 4 1.8228044691109062 9 5 1.5722264345281758 9 6 1.458219214673143 9 7 1.7810834831647497 9 8 2.062594293120874 9 9 1.9704647874907693 9 10 1.3430828834460584 9 11 3.6923312030193145 9 12 5.993709822053319 9 13 5.262753138920459 9 14 6.3335647754338815 9 15 6.04900891664839 9 16 6.959132515066802 9 17 6.061910221795717 9 18 5.572642645464843 9 19 4.637331204862825 9 20 4.489180060787156 9 21 2.7619971748762 9 22 2.930686327594259 9 23 2.8044857473524445 9 24 3.3541890947114794 9 25 3.076487198512517 9 26 2.297929793866196 9 27 2.9256332523561923 9 28 1.9216708595448893 9 29 1.8730722032038116 9 30 2.80786065611602 9 31 2.891089254757347 9 32 3.8069234386333974 9 33 2.5416996849147253 9 34 2.808918152813767 9 35 1.9810086188547587 9 36 1.5753658682276637 9 37 2.136995387981191 9 38 2.547995526452258 9 39 4.193435757434639 9 40 -1.502118029778483 9 41 -2.8358010137720804 9 42 5.938808684851274 9 43 9.303325722655241 9 44 8.79302316545056 9 45 4.9192783108209035 9 46 8.014683062648785 9 47 8.526437838289244 9 48 8.273536410710719 9 49 10.686981467894302 9 50 7.673745802738964 9 51 7.591695947763398 9 52 7.927224038837256 9 53 7.6276487296414714 9 54 7.853568218139824 9 55 7.172047807323395 9 56 7.274929719487883 10 1 1.1901226020715612 10 2 1.6614515692978742 10 3 2.3222966973834858 10 4 2.0309270986937165 10 5 1.4877605685357622 10 6 1.8734372930549341 10 7 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0.5695143335310462 119 35 0.8186114755540288 119 36 3.5859232350961605 119 37 3.5755918792092594 119 38 4.639751464971116 119 39 6.563564619412592 119 40 9.085834151559819 119 41 4.13392418417855 119 42 -0.12795113505246647 119 43 -1.2293555380185701 119 44 -3.0809094537079567 119 45 -3.8302947368425038 119 46 -7.111694828982948 119 47 -8.158238454211922 119 48 -10.171425551717476 119 49 -6.229335874133335 119 50 -6.860798522676344 119 51 -10.910865766256164 119 52 -11.874825332378393 119 53 -13.31692509429891 119 54 -12.041013368570313 119 55 -12.920484408171093 119 56 -10.420303939881782 120 1 -0.10898454078558473 120 2 0.2641301407264436 120 3 0.5031002688120552 120 4 -0.06505504416342944 120 5 -0.17072157432138368 120 6 -0.71808056408792 120 7 -0.26551496816268383 120 8 -0.1402541582065595 120 9 -0.17098985852693005 120 10 -0.8341848156689835 120 11 -12.199261717334664 120 12 -4.9422691602475695 120 13 -8.57795482568131 120 14 -6.614582348459923 120 15 -11.156466747068421 120 16 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-12.428767199562067 120 54 -12.503732666815928 120 55 -12.501624759048287 120 56 -8.934832448653712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 694.0 5 62.0 6 47.0 7 108.0 8 226.0 9 294.0 10 197.0 11 195.0 12 153.0 13 159.0 14 224.0 15 289.0 16 419.0 17 535.0 18 698.0 19 877.0 20 1152.0 21 1374.0 22 1669.0 23 2101.0 24 2250.0 25 2447.0 26 2613.0 27 2831.0 28 3162.0 29 3102.0 30 3366.0 31 3874.0 32 4538.0 33 5453.0 34 6406.0 35 7852.0 36 8924.0 37 8751.0 38 4900.0 39 1551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.55924448756183 0.33176433952546924 0.08144395338531374 0.027547219527385525 8 99.30533098583115 0.5186063502329537 0.11857281448744207 0.057489849448456753 9 96.07984089329787 2.2272542112956173 0.7260441377327295 0.9668607576737833 10 41.759189393122774 36.004215922292886 11.408141999928139 10.828452684656199 11 27.695200680312848 35.269669784767224 19.969817106035382 17.06531242888455 12 37.3097145868516 15.536631813445439 19.093816248068702 28.059837351634272 13 25.212892098738816 14.126932796761405 22.857005976548933 37.803169127950845 14 23.210820135611087 19.273067062797995 25.54448091621343 31.971631885377484 15 30.582885390669993 18.42844115953158 21.382463044730272 29.60621040506815 16 28.222086433509457 20.750512203012114 25.498723985478595 25.52867737799983 17 30.43306379155436 22.579215333932314 24.50074872716382 22.48697214734951 18 27.58087891818084 24.836804848542922 24.637976260345674 22.944339972930567 19 29.706030331344785 25.906226790292052 20.60662689570905 23.781115982654114 20 26.41403189284388 28.97791941725472 20.18162866761714 24.426420022284255 21 24.072299547229477 25.92410703591021 28.094530819538605 21.909062597321707 22 26.53396093085648 24.33976501024038 24.472710287089935 24.653563771813207 23 25.4919574096032 26.771106559831363 23.206735894027045 24.530200136538394 24 23.057022744421687 24.46911717149941 29.301857640760304 23.1720024433186 25 26.967530212113587 23.77684356772424 25.70395122944438 23.551674990717785 26 23.22305024540687 24.073874068473916 29.528026784749972 23.175048901369237 27 25.032947547504374 20.39513095152517 28.931540986749095 25.640380514221363 28 26.733386132910937 26.494588043295654 26.962584299325602 19.809441524467804 29 30.031749835260293 23.29119990415144 27.897921284370696 18.779128976217578 30 23.168539864362913 25.779433966785685 33.44436722662896 17.607658942222436 31 21.339977186768326 30.492885873806806 31.177282824037945 16.989854115386922 32 23.964734490482865 24.890693690779937 31.567662102753918 19.57690971598328 33 19.8612960896536 27.5644025293066 33.86727139651801 18.707029984521796 34 17.997194681884118 30.87408437533718 35.65631256518768 15.472408377591023 35 18.24696802646086 29.70615023249125 38.41738171707972 13.62950002396817 36 17.20977108028987 33.38292460526947 33.729663579210055 15.6776407352306 37 17.509755658281804 29.7184366932821 37.890376418322624 14.881431230113465 38 22.396420603123577 31.12779790322194 33.80394885205 12.67183264160449 39 17.65823393612928 25.358310888803388 30.67405732974221 26.309397845325126 40 11.730141720147861 19.295372722784794 27.560173869068404 41.41431168799894 41 14.224127520830818 21.421325926820433 30.21253471802922 34.142011834319526 42 17.65150696770012 31.093586835187786 36.41568542724425 14.839220769867847 43 10.221864333445508 16.913850295658865 29.775010816787656 43.08927455410797 44 11.157129269555377 19.11412369520461 25.627565947097846 44.10118108814217 45 15.667346204580884 30.023983691090063 39.684614462165726 14.624055642163327 46 15.559258238135765 30.038478597029595 36.931062177097445 17.471200987737195 47 14.954233957923996 28.654094982508266 33.9938659126851 22.39780514688264 48 17.58474830143913 32.335566127041574 35.97713683150995 14.102548740009347 49 11.69533701179271 20.64865904528352 25.387380239700914 42.26862370322286 50 17.975990396158466 30.47418967587035 36.881152460984396 14.668667466986795 51 16.67287652792635 33.78565161476425 35.21952861143496 14.321943245874449 52 14.908841639407349 32.84587261881855 37.192130075043295 15.053155666730806 53 14.675100218440193 33.08768813461677 37.521303919921266 14.71590772702177 54 13.952679610020507 30.587967237885096 31.405820911631032 24.053532240463372 55 15.151151631477926 34.66290786948177 35.26391554702496 14.922024952015356 56 18.459673838695352 31.982527930111722 33.59173436693747 15.966063864255457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 4.5 7 6.0 8 13.0 9 20.0 10 68.0 11 116.0 12 197.5 13 312.0 14 345.0 15 406.5 16 468.0 17 526.5 18 585.0 19 650.5 20 716.0 21 838.0 22 1211.0 23 1462.0 24 1786.0 25 2110.0 26 2509.5 27 2909.0 28 3258.0 29 3754.5 30 3902.0 31 3917.5 32 3933.0 33 3775.5 34 3618.0 35 3420.5 36 3223.0 37 2934.5 38 2451.0 39 2256.0 40 2137.5 41 2019.0 42 1973.5 43 1928.0 44 2131.0 45 2334.0 46 2706.5 47 2972.5 48 2866.0 49 2843.5 50 2821.0 51 2955.5 52 3090.0 53 3372.0 54 3830.0 55 4006.0 56 3977.0 57 3948.0 58 3967.0 59 3986.0 60 3807.0 61 3628.0 62 3462.0 63 3041.5 64 2787.0 65 2471.5 66 2156.0 67 1901.0 68 1646.0 69 1380.0 70 1114.0 71 982.5 72 700.0 73 549.0 74 421.0 75 293.0 76 208.5 77 124.0 78 103.5 79 63.0 80 43.0 81 30.5 82 18.0 83 13.0 84 8.0 85 4.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.03233804031475693 10 0.0 11 0.0023954103936856984 12 0.0 13 0.0 14 0.02275639874001413 15 0.17845807432958452 16 0.03593115590528548 17 0.021558693543171283 18 0.004790820787371397 19 0.01796557795264274 20 0.031140335117914076 21 0.008383936377899944 22 0.0 23 0.0 24 0.0 25 0.0 26 0.19402824188854154 27 0.03233804031475693 28 0.190435126298013 29 0.033535745511599775 30 0.041919681889499724 31 0.2491226809433126 32 0.014372462362114188 33 0.17965577952642736 34 0.09581641574742793 35 0.05868755464529961 36 0.17366725354221313 37 0.2491226809433126 38 0.15210855999904183 39 0.38925418897392594 40 0.5293856970045393 41 0.818032649443666 42 0.21558693543171284 43 0.3461368018875834 44 0.5198040554297966 45 0.12336363527481345 46 0.08383936377899945 47 0.029942629921071228 48 0.047908207873713964 49 0.3664977902339118 50 0.23115710299066988 51 0.34972991747811194 52 0.4084174721234115 53 0.2095984094474986 54 0.1245613404716563 55 0.15929479118009893 56 0.19163283149485585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 83493.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.77374151126442 #Duplication Level Percentage of deduplicated Percentage of total 1 94.12974465148378 81.67990130909179 2 3.8053830227743273 6.60414645539147 3 0.9454796411318152 2.461284179512055 4 0.39061421670117324 1.3558022828261052 5 0.20427881297446515 0.8863018456637083 6 0.1090407177363699 0.5677122633035105 7 0.09523809523809523 0.5784916100750962 8 0.0676328502415459 0.46950043716239687 9 0.037267080745341616 0.2910423628328123 >10 0.19737750172532784 3.14876696249985 >50 0.00966183574879227 0.5293856970045393 >100 0.008281573498964802 1.4276645946366762 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 297 0.3557184434623262 No Hit GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 257 0.30781023558861226 No Hit GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 240 0.2874492472422838 No Hit GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 142 0.17007413795168458 No Hit GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 128 0.1533062651958847 No Hit GATCTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 128 0.1533062651958847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.008383936377899944 0.0 0.0 0.0 0.0 11 0.016767872755799888 0.0 0.0 0.0 0.0 12 0.019163283149485587 0.0 0.0 0.0 0.0 13 0.022756398740014134 0.0 0.0 0.0 0.0 14 0.029942629921071228 0.0 0.0 0.0 0.0 15 0.03593115590528547 0.0 0.0 0.0 0.0 16 0.04191968188949972 0.0 0.0 0.0 0.0 17 0.043117387086342565 0.0 0.0 0.0 0.0 18 0.05509443905477106 0.0 0.0 0.0 0.0 19 0.063478375432671 0.0 0.0 0.0 0.0 20 0.0706646066137281 0.0 0.0 0.0 0.0 21 0.08024624818847088 0.0 0.0 0.0 0.0 22 0.09102559496005654 0.0 0.0 0.0 0.0 23 0.10420035212532787 0.0 0.0 0.0 0.0 24 0.11378199370007067 0.0 0.0 0.0 0.0 25 0.12815445606218484 0.0 0.0 0.0 0.0 26 0.13534068724324194 0.0 0.0 0.0 0.0 27 0.1461200340148276 0.0 0.0 0.0 0.0 28 0.15210855999904183 0.0 0.0 0.0 0.0 29 0.16288790677062748 0.0 0.0 0.0 0.0 30 0.1688764327548417 0.0 0.0 0.0 0.0 31 0.17486495873905597 0.0 0.0 0.0 0.0 32 0.17486495873905597 0.0 0.0 0.0 0.0 33 0.17486495873905597 0.0 0.0 0.0 0.0 34 0.17486495873905597 0.0 0.0 0.0 0.0 35 0.1760626639358988 0.0 0.0 0.0 0.0 36 0.1760626639358988 0.0 0.0 0.0 0.0 37 0.1760626639358988 0.0 0.0 0.0 0.0 38 0.1760626639358988 0.0 0.0 0.0 0.0 39 0.1760626639358988 0.0 0.0 0.0 0.0 40 0.1760626639358988 0.0 0.0 0.0 0.0 41 0.1808534847232702 0.0 0.0 0.0 0.0 42 0.18444660031379875 0.0 0.0 0.0 0.0 43 0.1880397159043273 0.0 0.0 0.0 0.0 44 0.19881906267591296 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAAA 15 0.00807893 50.500607 38 AACCATA 20 3.9615243E-4 50.500603 38 TTCGTTT 20 3.9615243E-4 50.500603 38 ACCAACC 40 2.408342E-9 50.500603 38 CAAACCA 80 0.0 50.469944 39 AGATCCA 15 0.008098366 50.469944 39 GCGCCCT 15 0.008098366 50.469944 39 TCGTCTC 20 3.9734284E-4 50.469944 40 ACTAAAA 15 0.008098366 50.469944 39 CGTCTCA 40 2.4338078E-9 50.43932 41 AGCCATC 15 0.008137341 50.408733 37 TGCCCGT 20 3.9973218E-4 50.40873 37 TTTCGTT 20 3.9973218E-4 50.40873 37 AAACCAT 20 3.9973218E-4 50.40873 37 ATGCTCG 20 4.009311E-4 50.37818 36 TATGCCT 30 9.835258E-7 50.37818 35 GTTGTCG 15 0.008156881 50.37818 36 TGATAAC 15 0.008255104 50.225983 34 CCGATTC 20 4.0940396E-4 50.16536 43 ATAGAAT 15 0.008294638 50.16536 42 >>END_MODULE