FastQCFastQC Report
Fri 17 Jun 2016
SRR1526290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160575
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG18531.153977891950802No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG13610.8475790129223105No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13500.8407286314806165No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG9110.567336135762105No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG7800.48575432041102284No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG5300.3300638330997976No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA4710.2933208780943484No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG3210.19990658570761327No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG3180.19803829985987853No Hit
TAGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA2490.15506772536198038No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2470.15382220146349057No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2350.14634905807255177No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2210.13763039078312317No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG2180.13576210493538846No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG1980.12330686595049042No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG1880.11707924645804142No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA1790.11147438891483731No Hit
TAGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACCAAAAAAAAAAAAAAA1660.10337848357465358No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1630.10151019772691888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG150.0080022450.64426438
CGGCCAT150.0080022450.64426440
GCTTACC203.9173928E-450.64426438
ACTAACA150.0080022450.64426438
CCAACGG150.0080022450.64426439
ATGCCAT451.1823431E-1050.64426440
TCAACAA150.0080022450.64426440
GAACCGA150.00805252450.56410237
AAATCCG204.003999E-450.42045635
ATATGCG150.0082047950.3251434
TTAAGAC204.0415788E-450.3251434
TTGAAAC150.0082047950.3251434
ATTCTGA150.0082047950.3251434
CGATTTA150.008297170550.18284649
AGATTTA150.008297170550.18284649
CGATCAA150.008297170550.18284649
TCCTATG150.00831780550.15133747
ACGATTC1650.050.15133748
AACGATT3600.050.15133747
CACAAAC204.111202E-450.15133747
Produced by FastQC (version 0.11.5)