##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526290_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 160575 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.65784212984587 39.0 38.0 39.0 35.0 40.0 2 36.015164253464114 38.0 36.0 39.0 29.0 40.0 3 36.829655924023044 38.0 37.0 39.0 32.0 40.0 4 36.88483263272614 39.0 37.0 39.0 32.0 40.0 5 37.17403705433598 39.0 38.0 39.0 33.0 40.0 6 36.999489335201616 38.0 37.0 39.0 33.0 40.0 7 36.86685349525144 38.0 37.0 39.0 33.0 40.0 8 36.80820800249105 38.0 36.0 39.0 33.0 40.0 9 36.77594270590067 38.0 36.0 39.0 33.0 40.0 10 36.46791219056516 38.0 36.0 39.0 32.0 40.0 11 34.67598318542737 38.0 33.0 39.0 27.0 40.0 12 32.94832321345166 36.0 31.0 39.0 19.0 40.0 13 32.79832477035653 37.0 31.0 39.0 18.0 40.0 14 31.96079090767554 36.0 29.0 39.0 15.0 40.0 15 32.68036742955005 36.0 30.0 39.0 18.0 40.0 16 32.31366028335669 36.0 29.0 39.0 17.0 40.0 17 32.430733302195236 36.0 30.0 39.0 18.0 40.0 18 32.73677097929316 36.0 30.0 38.0 20.0 40.0 19 33.54564533706991 36.0 31.0 38.0 24.0 40.0 20 33.57142145414915 36.0 31.0 38.0 24.0 40.0 21 35.080778452436554 38.0 35.0 39.0 27.0 40.0 22 34.84919196637085 38.0 34.0 39.0 26.0 40.0 23 35.005480305153355 38.0 34.0 39.0 27.0 40.0 24 34.62912657636618 38.0 33.0 39.0 25.0 40.0 25 34.80775961388759 38.0 34.0 39.0 27.0 40.0 26 35.170649229332085 38.0 35.0 39.0 27.0 40.0 27 35.22484820177487 38.0 35.0 39.0 28.0 40.0 28 34.884085318387044 38.0 35.0 39.0 27.0 40.0 29 34.794818620582284 38.0 35.0 39.0 27.0 40.0 30 34.45073641600498 38.0 34.0 39.0 25.0 40.0 31 33.999177954226994 38.0 33.0 39.0 23.0 40.0 32 33.15126264985209 38.0 32.0 39.0 18.0 40.0 33 34.39004203643157 38.0 34.0 39.0 25.0 40.0 34 34.136584150708394 38.0 33.0 39.0 24.0 40.0 35 34.17841507083917 38.0 34.0 39.0 25.0 40.0 36 32.141036898645496 35.0 31.0 38.0 19.0 39.0 37 31.93761793554414 35.0 31.0 38.0 17.0 39.0 38 31.187806321033786 35.0 30.0 38.0 11.0 39.0 39 29.408027401525768 34.0 27.0 38.0 4.0 39.0 40 25.411097617935543 31.0 12.0 37.0 4.0 39.0 41 22.244757901292232 26.0 9.0 33.0 4.0 37.0 42 25.85759302506617 30.0 21.0 34.0 4.0 37.0 43 21.773084228553635 26.0 8.0 33.0 4.0 37.0 44 23.40406040790908 28.0 10.0 33.0 4.0 37.0 45 25.602945664019927 30.0 20.0 35.0 4.0 38.0 46 25.450823602677875 30.0 16.0 36.0 4.0 38.0 47 24.68480772224817 30.0 10.0 35.0 4.0 38.0 48 24.552227930873425 30.0 11.0 35.0 4.0 38.0 49 22.288712439669936 27.0 5.0 35.0 4.0 38.0 50 24.471642534641134 30.0 9.0 35.0 4.0 38.0 51 25.81365094192745 32.0 4.0 38.0 4.0 39.0 52 25.732947220924803 32.0 4.0 38.0 4.0 39.0 53 25.255326171570918 32.0 4.0 38.0 4.0 39.0 54 24.438330997976024 31.0 4.0 37.0 4.0 39.0 55 24.518997353261717 31.0 4.0 37.0 4.0 39.0 56 24.10537754943173 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9097066993871152 1 2 2.885208174492803 1 3 1.7487757044152517 1 4 1.2508130982978969 1 5 1.4694710089575054 1 6 1.3844786564373806 1 7 1.8687097529753132 1 8 1.9095512498704537 1 9 1.845289977824308 1 10 1.7546818513726024 1 11 -3.827643812980906 1 12 -6.749781186450541 1 13 -21.51397957763119 1 14 -18.050519581457365 1 15 -11.197336079582424 1 16 -21.03191635313049 1 17 -21.11041987782336 1 18 -20.114687231868075 1 19 -6.173297475710793 1 20 -10.25436089026751 1 21 -8.557855279302945 1 22 -3.0554592495208475 1 23 -3.6852110899982833 1 24 -3.315855740624304 1 25 -3.9142797919313708 1 26 2.968537683937363 1 27 2.6406424200830827 1 28 2.410562202551503 1 29 2.1012992381234525 1 30 2.297358139833044 1 31 1.3336029227085646 1 32 -11.201183383401599 1 33 0.47512599363737706 1 34 0.3571854633204268 1 35 0.4952009774787811 1 36 3.571565090710365 1 37 3.5845831676131823 1 38 4.3888708163004395 1 39 6.204899106829828 1 40 8.452712433338672 1 41 -3.3664706579862056 1 42 -7.812127813653511 1 43 -12.994226861846684 1 44 -16.412002556334347 1 45 -13.863925960919069 1 46 -17.832284808097665 1 47 -17.928267088576156 1 48 -15.78890293185906 1 49 -14.453692889843241 1 50 -15.875383711336163 1 51 -17.21562870963112 1 52 -17.004859353348788 1 53 -16.778687165624333 1 54 -18.007247060363426 1 55 -16.20459897058055 1 56 -15.601272247462745 2 1 0.7272677851046225 2 2 2.6249519812757143 2 3 1.5463040606028216 2 4 1.0536458955441006 2 5 1.3843742311232958 2 6 1.2703203132287584 2 7 1.7661760359164589 2 8 1.7243754697226024 2 9 1.6670833303439139 2 10 1.488190118400425 2 11 -3.221103550016064 2 12 -11.098322465159416 2 13 -12.217523395700148 2 14 -15.412209102364761 2 15 -7.996388051818755 2 16 -3.560667552272754 2 17 -2.3692088852157234 2 18 -9.775710876131946 2 19 -1.9109815793166867 2 20 -3.0686490229913943 2 21 2.4908440040332067 2 22 3.011213060168224 2 23 2.578995218900765 2 24 2.9123198702328708 2 25 2.4616019303190697 2 26 2.5725103717089297 2 27 2.383066662507325 2 28 2.3705813888276737 2 29 2.936466553737702 2 30 2.3403015403183502 2 31 -1.4781740650460762 2 32 -9.570011327648537 2 33 -1.3052475744456942 2 34 -1.087682207804228 2 35 -1.336890890925936 2 36 3.9487729232254623 2 37 3.776163778188213 2 38 4.837745033331082 2 39 6.987191302529844 2 40 8.695136675762914 2 41 4.371636672299896 2 42 0.6884647036987843 2 43 -4.414462740183122 2 44 -10.983866446633991 2 45 -2.650337562973128 2 46 -12.068163144534662 2 47 -12.73631968154495 2 48 -9.39386314855112 2 49 -11.981682280961124 2 50 -9.160694924021678 2 51 -8.58679850817522 2 52 -9.119920580141859 2 53 -8.817736880651701 2 54 -14.356437286648625 2 55 -8.802025752364875 2 56 -7.9753482025442874 3 1 1.1250425249846288 3 2 2.936222582020001 3 3 1.6275298523549324 3 4 1.850258639525272 3 5 1.7292895497228287 3 6 1.7399413354813618 3 7 2.217493724076249 3 8 2.224185601111685 3 9 2.2663846138557275 3 10 1.9813168746850778 3 11 0.820211859720402 3 12 1.2195555632449242 3 13 -5.853165531342281 3 14 -6.120456994823179 3 15 -2.9979829481504936 3 16 3.010571150544898 3 17 2.346861594012651 3 18 2.2486302797881557 3 19 1.1843704370879635 3 20 0.7035086439031133 3 21 3.0737026658392423 3 22 3.789712338667499 3 23 3.1583223245436542 3 24 3.358966790564004 3 25 2.980247027911531 3 26 2.7437718587598923 3 27 2.509063472714665 3 28 2.497679436978352 3 29 2.736952617381654 3 30 2.7787081629354944 3 31 2.806363035280498 3 32 1.917647212641583 3 33 2.6661202390273857 3 34 2.833978003329662 3 35 2.4216721834266153 3 36 4.525024157371433 3 37 4.983310432703284 3 38 5.716988596785171 3 39 7.503823792846546 3 40 10.25346431303792 3 41 9.06974558093512 3 42 6.991039322062875 3 43 3.2806082706773623 3 44 3.444363218437779 3 45 7.350611780081476 3 46 0.9953289189574015 3 47 -4.296394110040431 3 48 0.6887296971996193 3 49 -10.504770304049147 3 50 -0.739604318720545 3 51 -3.094886911000465 3 52 -2.895647776921688 3 53 -3.49765637636067 3 54 -5.0333948320272235 3 55 -3.05447505744576 3 56 -0.033372050041819534 4 1 1.157525417609449 4 2 2.7748782098536253 4 3 1.9841658243072828 4 4 1.6023917813686381 4 5 1.8442539269580465 4 6 1.7729874338734106 4 7 2.197795602367485 4 8 2.1335798733090314 4 9 2.134453662882791 4 10 1.8849101274799835 4 11 3.590325345682494 4 12 2.673837335090269 4 13 4.350686796105549 4 14 3.877712586433333 4 15 5.443176129318459 4 16 6.157525615358615 4 17 5.907490694854644 4 18 5.512224658994601 4 19 4.467465815984646 4 20 4.287496703940846 4 21 3.042881230227742 4 22 3.5253998721078403 4 23 2.8467287338252873 4 24 3.5729145813893837 4 25 2.441399910117049 4 26 2.5038462019211707 4 27 2.217421446300314 4 28 1.9787416430553435 4 29 2.5621617075227405 4 30 2.9461351573879213 4 31 2.900840802732837 4 32 3.6780876395515563 4 33 2.508394463915451 4 34 2.938818756898982 4 35 2.8872039904723152 4 36 3.9347563530291154 4 37 4.455882311839325 4 38 5.2696483360209 4 39 7.165604649482518 4 40 9.171813556934179 4 41 5.351207663106841 4 42 7.74362302852003 4 43 7.6105060488306115 4 44 10.036222079072513 4 45 7.9646891082333156 4 46 7.499876805527762 4 47 7.889886197469917 4 48 7.4187006921026075 4 49 2.589932745710083 4 50 8.18330689189025 4 51 6.667031931048477 4 52 6.9181000758563265 4 53 5.833494534624659 4 54 3.4752503764997122 4 55 6.404988207458075 4 56 6.625832140209088 5 1 1.5050878265229954 5 2 3.367735464644973 5 3 2.2300875017084394 5 4 1.8999330347182735 5 5 2.1235230414018176 5 6 2.199232869756379 5 7 2.3555847675846024 5 8 2.266765901234372 5 9 2.4383102438302444 5 10 2.069167253770864 5 11 4.331726000721439 5 12 6.3621610308889345 5 13 6.232309662919519 5 14 6.646051326188555 5 15 6.57810161472112 5 16 6.887580619824789 5 17 6.407216456063175 5 18 6.130421578117666 5 19 5.07627593304543 5 20 4.971416908287928 5 21 3.6351743366480633 5 22 3.414936486904203 5 23 3.393795945835379 5 24 4.064616899098937 5 25 3.5055561165326665 5 26 2.8384331983012174 5 27 3.0088432569031696 5 28 2.5730876227146595 5 29 2.90041566831443 5 30 2.9503349285944154 5 31 3.1365294555151877 5 32 4.3778353398634025 5 33 3.1600328448096207 5 34 2.5795972816509902 5 35 2.609878053750961 5 36 3.484189854039883 5 37 3.7332239193487666 5 38 5.180904663101586 5 39 5.948266489546455 5 40 4.528026240870052 5 41 2.6887407676466673 5 42 7.458085598842693 5 43 11.434444744707626 5 44 10.20708068155163 5 45 8.51846341754333 5 46 9.09036777131843 5 47 9.440447995222726 5 48 9.482198725418705 5 49 9.345797538150496 5 50 9.87852573444584 5 51 8.538280736150885 5 52 8.341556111460356 5 53 8.681143132872663 5 54 8.281151995292124 5 55 8.341881943844076 5 56 8.54736850427284 6 1 1.6551177129545493 6 2 3.5421491631787774 6 3 2.1224436073306023 6 4 2.1782108730502614 6 5 2.3071312127038084 6 6 2.2061067490151984 6 7 2.777422958928085 6 8 2.7467419920891274 6 9 2.7887198637451576 6 10 2.4171859591609746 6 11 4.352277317482454 6 12 6.710267637548334 6 13 5.903136989666123 6 14 7.479056494783187 6 15 6.770482332698691 6 16 6.8356485499257005 6 17 6.656680258320478 6 18 6.292195074126948 6 19 5.02293744283763 6 20 4.884623194986702 6 21 3.6174886012660394 6 22 3.819124103373383 6 23 3.6687159556803195 6 24 3.844371478755072 6 25 3.389579181825731 6 26 2.5575710626519736 6 27 2.8969449410914834 6 28 2.550532157013741 6 29 2.571981365723097 6 30 3.078354337194675 6 31 3.5613387097608182 6 32 4.341795819452727 6 33 3.149976939602354 6 34 3.372677693732143 6 35 2.953958782276679 6 36 3.763856532426715 6 37 3.0969769519425583 6 38 4.109071745834264 6 39 5.695110833978788 6 40 2.8014519291369915 6 41 0.14028958801163327 6 42 6.2838301225936135 6 43 10.850709995577848 6 44 9.14294791547449 6 45 6.7672415899001415 6 46 9.57936253240278 6 47 9.814397575054656 6 48 9.838884495961231 6 49 10.46581793124497 6 50 9.593655300916783 6 51 9.896267445478973 6 52 9.635533116488311 6 53 9.839804923948925 6 54 10.606808617773751 6 55 9.265073814734691 6 56 8.63381061249688 7 1 1.665231492979835 7 2 2.9015230012095685 7 3 2.1811927707127694 7 4 1.9352942373694404 7 5 1.9690260591379598 7 6 2.1251965088129197 7 7 2.4403961127914044 7 8 2.4439607025821743 7 9 2.394803004966249 7 10 1.8867330700407194 7 11 4.19272500098063 7 12 5.62340811497824 7 13 6.084255454751272 7 14 6.921794651992975 7 15 6.074676576767999 7 16 6.43767874253372 7 17 6.733946563072472 7 18 5.765497669501382 7 19 4.823933947634238 7 20 5.0309755412349375 7 21 3.4549988265951086 7 22 3.9439474840530906 7 23 3.281417673535607 7 24 3.602495967488615 7 25 2.9531993201465454 7 26 2.462382544766946 7 27 2.4503769333574184 7 28 2.808545096702886 7 29 2.5323814549623336 7 30 2.73392807111302 7 31 2.8062632282407733 7 32 4.207453586984521 7 33 2.5621878416622934 7 34 3.215839737310638 7 35 2.435478823921656 7 36 0.5474142032826705 7 37 0.8192941974248242 7 38 1.8346610966188237 7 39 3.093117824385569 7 40 -3.1313583631215067 7 41 -9.602887328772779 7 42 3.3910659372734706 7 43 8.022607072992887 7 44 6.528201131805268 7 45 3.778284945295397 7 46 6.14766773058825 7 47 6.418324101417415 7 48 6.524613654881435 7 49 8.161474955116468 7 50 6.637865905512605 7 51 7.633904093058355 7 52 7.737860773214717 7 53 8.123176679730886 7 54 8.37222097740787 7 55 7.64434056568286 7 56 8.022819313322344 8 1 1.3398250760760106 8 2 2.7295219096341867 8 3 2.0740039671573527 8 4 2.075093855318052 8 5 2.0767606503819636 8 6 2.0390205312681857 8 7 2.4911348754127403 8 8 2.583117070754952 8 9 2.3447854617905435 8 10 2.368468674890437 8 11 4.343596312040624 8 12 6.226445033616891 8 13 6.725185632911966 8 14 7.518574504630301 8 15 6.553605273504971 8 16 6.987501361534925 8 17 6.62954517521235 8 18 5.879430849494366 8 19 5.330307968140261 8 20 5.011580585872572 8 21 3.452308872987068 8 22 3.7689445601904694 8 23 3.504683823003461 8 24 3.8662868528342145 8 25 3.5045423477088207 8 26 2.2034452713662986 8 27 2.477646825369426 8 28 2.280495507992896 8 29 2.777030786372414 8 30 2.7504381486928438 8 31 3.0451428040857706 8 32 3.6777081579564097 8 33 2.3462898259903895 8 34 1.8484856380552444 8 35 1.656990655819051 8 36 -0.05885845665261513 8 37 0.675420664229236 8 38 1.5722931577470476 8 39 2.846889607162069 8 40 -1.7631465974983342 8 41 -9.564019448378252 8 42 4.159963835990869 8 43 8.081376711603468 8 44 6.3517362766339325 8 45 4.221152669750584 8 46 7.374812871128579 8 47 7.7884656163681 8 48 7.54693247286124 8 49 9.573895523515361 8 50 7.690904773392997 8 51 8.92775228611126 8 52 8.493971646085171 8 53 9.37778663645772 8 54 9.484126945986095 8 55 9.13322988773658 8 56 7.793530006862557 9 1 1.5525203103571528 9 2 3.3390933954171302 9 3 1.6675161818149462 9 4 2.092534784432992 9 5 2.1293237260727906 9 6 2.2087041516126007 9 7 2.7990043187447426 9 8 2.3460926414397747 9 9 2.5135479768085673 9 10 2.1134426436529523 9 11 4.185967157054641 9 12 6.040061373224418 9 13 5.8390422608655115 9 14 7.14991210975645 9 15 6.632399826969127 9 16 6.638322346717146 9 17 6.693922427915588 9 18 5.860147709726643 9 19 5.320836602501494 9 20 4.478320673978303 9 21 3.594379357568563 9 22 3.809192858148023 9 23 2.9355608754664217 9 24 3.873936031849034 9 25 3.0989756676928053 9 26 2.4608942100927678 9 27 3.07700605644672 9 28 2.4594702777165622 9 29 2.481416048687194 9 30 2.7862611362779433 9 31 3.000108763236753 9 32 4.232513924876713 9 33 2.8442473750492567 9 34 2.7082773881553663 9 35 2.5868464828114384 9 36 1.9462476707002097 9 37 2.346365798390231 9 38 2.963502609088657 9 39 4.599312514651054 9 40 -0.1291057484795104 9 41 -2.2480602973970747 9 42 5.971798037032116 9 43 10.517860492140112 9 44 8.043406280638738 9 45 6.054712942077376 9 46 8.545978269606753 9 47 9.267720722495454 9 48 8.981894427206456 9 49 10.776398527119685 9 50 9.173143459816707 9 51 8.701080279703572 9 52 8.947756187534907 9 53 8.665091402176582 9 54 8.533966753755415 9 55 8.967671217332093 9 56 8.34232856513248 10 1 1.535064057689631 10 2 3.265725917824298 10 3 2.2483953964969743 10 4 2.129901408419336 10 5 2.307407379508561 10 6 2.0280383385524203 10 7 2.648236072906073 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114 41 9.33550613616936 114 42 5.946273561507642 114 43 2.855622729902347 114 44 3.5399097771422348 114 45 4.8938737812382165 114 46 -1.7673084436799584 114 47 -5.421090466315736 114 48 -2.9628608175487905 114 49 -13.803671402950245 114 50 -1.553080316407069 114 51 -2.693325314702026 114 52 -2.640355700576979 114 53 -3.6342092971241193 114 54 -4.8328164630278 114 55 -2.9854756358147583 114 56 0.07414674695066381 115 1 0.6747864500145937 115 2 2.3717679672221337 115 3 1.602957552038923 115 4 1.3799524392419542 115 5 1.1131840686287902 115 6 1.0197713452884898 115 7 1.3638923292849228 115 8 1.568688161861381 115 9 1.439371428718971 115 10 1.3276718926648101 115 11 -1.0305018811535263 115 12 -2.8422838178164227 115 13 -0.7941593201621586 115 14 -7.101932422522864 115 15 -1.0925672349544584 115 16 -9.404760657235421 115 17 -8.812994568131842 115 18 -1.8281922112219746 115 19 -2.5624967308318354 115 20 -2.668780775297062 115 21 0.20669819814827406 115 22 -0.07157130522483612 115 23 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1.4247782715273374 116 6 1.2526435455519902 116 7 1.465670985411407 116 8 1.4415471868943186 116 9 1.5256691889297755 116 10 1.2331396133499197 116 11 -1.4458510247635346 116 12 -3.5250901419270946 116 13 -5.144291072467823 116 14 -9.457663647819306 116 15 -5.428206233636935 116 16 -9.972283713888913 116 17 -10.45759272359956 116 18 -3.9701553205763886 116 19 -4.236739155074261 116 20 -4.773194477536851 116 21 1.3216520848412898 116 22 1.4001019490571096 116 23 1.2810154209209657 116 24 1.3996936076066078 116 25 1.5323090010261353 116 26 1.5169548161533797 116 27 1.7542787837194496 116 28 1.4412884595347464 116 29 1.7192948365659788 116 30 1.9892914393082464 116 31 0.7213208844488719 116 32 -8.105364863002073 116 33 0.9927322235341052 116 34 0.9931258730038479 116 35 0.11260405856901201 116 36 2.688671913124452 116 37 2.8645476165720467 116 38 3.042290487876535 116 39 5.229615544954086 116 40 7.664833645459883 116 41 5.258000308663533 116 42 2.696040461274542 116 43 -10.060927386647768 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1.0841804157344441 118 9 0.963234497184736 118 10 0.8786464054084391 118 11 -5.792246009089556 118 12 -6.582038940255202 118 13 -6.648732033805338 118 14 -0.454006385541355 118 15 -16.68264719497445 118 16 -6.847414330398841 118 17 -13.680685722554628 118 18 -3.3113256445053345 118 19 -3.6084737423260904 118 20 -3.613058636366528 118 21 0.5857586679133888 118 22 0.649318249997549 118 23 0.46283360273914553 118 24 -2.370421334922124 118 25 0.151170025059578 118 26 1.6232766552338376 118 27 1.5630562131865275 118 28 1.5102539767761272 118 29 1.8953658919473853 118 30 1.9386122334566238 118 31 -0.4422109859795498 118 32 -8.941310526533943 118 33 0.7085106979332707 118 34 1.1540454132337388 118 35 0.2445267335951371 118 36 2.6821410876281107 118 37 2.772071127543832 118 38 4.130587248587091 118 39 5.604484928444158 118 40 8.340643467821433 118 41 8.056328417336466 118 42 2.5601992909506137 118 43 0.5098667617325887 118 44 1.3068859044631864 118 45 3.179948051795243 118 46 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119 29 1.1208770301835713 119 30 1.4197687790776214 119 31 -0.7114358868034714 119 32 -9.531484466112826 119 33 -0.17232543267399336 119 34 -0.24433725383980942 119 35 0.21423988586855813 119 36 2.658033672309216 119 37 2.266196852292083 119 38 3.7638646364418307 119 39 5.34975499252716 119 40 8.337668185551063 119 41 7.379413556182975 119 42 2.7538977956805475 119 43 1.133226542019436 119 44 1.2140418397698696 119 45 2.9198778656493793 119 46 -4.467376517199362 119 47 -7.055127226016213 119 48 -9.550607584056618 119 49 -5.351799381166719 119 50 -3.924080997142262 119 51 -5.175830420215981 119 52 -4.66036931528086 119 53 -10.142310045932831 119 54 -8.844593181884166 119 55 -10.492827575910061 119 56 -7.379835553934294 120 1 0.3164933743302143 120 2 2.051719658043389 120 3 0.9141491784479427 120 4 0.8272149191131248 120 5 0.8229264196754826 120 6 0.22991627282471683 120 7 0.5321693019097253 120 8 0.6528266481737859 120 9 0.2723021855627721 120 10 0.2684931487034561 120 11 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49 -15.422759722038563 120 50 -11.322311085637839 120 51 -11.134778306155017 120 52 -10.810156270377549 120 53 -11.63591869883352 120 54 -11.095494525705863 120 55 -11.340746291085415 120 56 -7.683260660456744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1228.0 5 84.0 6 153.0 7 171.0 8 380.0 9 460.0 10 322.0 11 307.0 12 287.0 13 343.0 14 416.0 15 566.0 16 699.0 17 1087.0 18 1339.0 19 1597.0 20 1966.0 21 2620.0 22 3235.0 23 3886.0 24 4381.0 25 4845.0 26 5074.0 27 5217.0 28 5545.0 29 5866.0 30 6665.0 31 7910.0 32 9382.0 33 11361.0 34 13166.0 35 15549.0 36 17234.0 37 16370.0 38 8495.0 39 2369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.20846956250972 0.572940993305309 0.1581815351082049 0.0604079090767554 8 98.30172816440916 1.271679900358088 0.20675696714930716 0.21983496808345007 9 93.31381587143392 3.871309331007849 1.7054939578921142 1.1093808396661269 10 38.907675541024446 35.74529036275884 17.274170948155067 8.072863148061654 11 26.311561168821463 36.820242632588496 16.862217572179457 20.00597862641058 12 28.5436711816908 18.25813482796201 17.62540868752919 35.572785302818 13 20.069126576366184 13.839638798069437 21.357621049353885 44.733613576210495 14 24.726862752426157 16.879695033075457 29.36614717643981 29.027295038058575 15 31.95344514648188 17.144959988024475 19.000542640977503 31.901052224516146 16 33.528737493926066 23.95123409875282 20.159853478027934 22.36017492929318 17 31.157068552029155 19.885383249758622 28.40315196063164 20.55439623758059 18 27.324987543597405 22.626432486297958 30.023044344793227 20.02553562531141 19 38.71570489863287 23.966864937248918 17.61452461773224 19.702905546385974 20 21.365338250777178 38.45573989047889 19.700094071032975 20.47882778771096 21 21.08734647101323 23.604848152669476 36.28391340093176 19.023891975385535 22 26.81301572473922 22.246925112875605 20.63272614043282 30.30733302195236 23 21.327105713840886 29.854585084851315 19.529191966370853 29.289117234936946 24 27.127510509107893 22.58321656546785 29.12906741398101 21.16020551144325 25 32.34002802428771 27.555970730188385 19.643780165031917 20.460221080491984 26 23.295380104189412 21.81614000062389 23.546807249586674 31.341672645600028 27 28.89571354708495 20.60126207725707 18.82214428545621 31.680880090201768 28 28.11747532843437 33.05490748942647 19.813980761793072 19.01363642034609 29 35.89579063436392 25.22566859577755 20.189007182771316 18.689533587087208 30 23.37253778204154 24.85890136177317 31.51389806012733 20.25466279605796 31 20.375224685440383 38.679598562013176 23.37352706211304 17.571649690433393 32 30.599915294352126 26.476120481327385 21.513366052965942 21.410598171354543 33 20.714548665056874 29.740623576611817 22.71056785778909 26.83425990054221 34 19.186731405705597 38.73060789458922 24.57881188489083 17.50384881481435 35 19.589273252915962 31.45623566942478 34.58777788854551 14.366713189113748 36 20.200338054874663 40.03143536103887 22.854255936231123 16.913970647855347 37 20.45644655854776 30.21836023821117 33.70787919647428 15.617314006766794 38 31.074154444777015 30.65137683328345 22.613613688516413 15.660855033423127 39 18.199149893736717 25.712589073634206 21.142642830353793 34.945618202275284 40 13.005506932219 17.67919457200674 24.483607635778142 44.831690859996115 41 15.128256664070546 20.397331859407583 26.911288093862275 37.5631233826596 42 26.31762892324207 30.07907333786845 26.61282772996486 16.990470008924614 43 11.601629877759168 16.094417918656102 26.251781116416268 46.05217108716847 44 11.890379402945834 18.746674512209626 18.935092738073628 50.42785334677091 45 18.85177426996833 28.45689633674971 34.90561332635097 17.785716066931 46 17.975994615614717 30.106441320171502 25.790831588393658 26.12673247582012 47 16.384751010846742 33.37632157698322 26.044022453569582 24.194904958600453 48 19.664163992647747 38.09402162061124 25.370883828156643 16.87093055858438 49 12.507029580474638 23.568152563766105 18.183806345992824 45.74101150976643 50 26.45746347369669 29.89446223170876 26.608499191771674 17.03957510282287 51 18.604520374177504 38.740619005067764 26.51236322962426 16.14249739113047 52 17.682286457291458 31.51380276055211 33.544833966793355 17.259076815363073 53 17.93012648117758 31.137956208934174 33.82919113196598 17.102726177922264 54 16.214008194625475 33.46014006947346 23.25552388851817 27.070327847382895 55 17.693032191263885 39.66929261553239 26.00313117893253 16.634544014271192 56 26.503821556699425 29.902979254406485 25.621587895804087 17.97161129309 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 8.0 7 16.0 8 33.0 9 50.0 10 136.5 11 223.0 12 473.0 13 876.0 14 1029.0 15 938.0 16 847.0 17 821.5 18 796.0 19 883.5 20 971.0 21 1024.0 22 1198.0 23 1319.0 24 1499.0 25 1679.0 26 1935.5 27 2192.0 28 2478.5 29 3038.5 30 3312.0 31 3549.0 32 3786.0 33 4157.0 34 4528.0 35 4839.0 36 5150.0 37 4977.0 38 4465.5 39 4127.0 40 3790.0 41 3453.0 42 3360.0 43 3267.0 44 3455.0 45 3643.0 46 5029.5 47 8568.5 48 10721.0 49 9398.0 50 8075.0 51 7420.5 52 6766.0 53 6848.5 54 7246.0 55 7561.0 56 7697.0 57 7833.0 58 8113.0 59 8393.0 60 8472.5 61 8552.0 62 8576.0 63 8162.5 64 7725.0 65 7180.0 66 6635.0 67 5780.5 68 4926.0 69 4355.5 70 3785.0 71 3280.5 72 2454.5 73 2133.0 74 1748.5 75 1364.0 76 1052.5 77 741.0 78 577.0 79 344.5 80 276.0 81 207.5 82 139.0 83 89.5 84 40.0 85 25.5 86 11.0 87 7.5 88 3.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02179666822357154 10 0.0 11 0.0018682858477347035 12 0.0 13 0.0 14 0.020551144325081736 15 0.15444496341273548 16 0.03300638330997976 17 0.024910477969796042 18 0.009341429238673517 19 0.012455238984898021 20 0.036120193056204265 21 0.01183247703565312 22 0.0 23 0.0 24 0.0 25 0.0 26 0.18060096528102135 27 0.028647049665265455 28 0.1675229643468784 29 0.03238362136073486 30 0.030515335513000154 31 0.21858944418496032 32 0.01183247703565312 33 0.19056515646893973 34 0.08469562509730655 35 0.049198193990347194 36 0.15319943951424567 37 0.23727230266230734 38 0.12891172349369454 39 0.3705433598007162 40 0.5966059473766153 41 0.8488245368208003 42 0.214230110540246 43 0.3506149774248793 44 0.5131558461777985 45 0.10649229332087809 46 0.06912657636618402 47 0.04110228865016347 48 0.049820955939592085 49 0.3344231667445119 50 0.2167211583372256 51 0.3387825003892262 52 0.378016503191655 53 0.19554725206289897 54 0.14074420052934766 55 0.1569360112097151 56 0.18682858477347034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 160575.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.9625904940649 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4846133900186 66.74954383283772 2 5.039049008333204 7.353241385490787 3 1.3591262357502025 2.9749611290634577 4 0.6375632554422008 1.8607306688356875 5 0.3395020614204972 1.2385474939657302 6 0.23562211857169876 1.0314960089214515 7 0.16173672459228128 0.826051128299458 8 0.11281588239065729 0.6585071222476891 9 0.06988589325526498 0.4589150229806247 >10 0.490199547940402 6.529704175446235 >50 0.03793805038327156 1.8808228624351986 >100 0.025957613420133176 3.4950379914655634 >500 0.0029951092407845973 1.6178573111807033 >1k 0.0029951092407845973 3.3245838668296854 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 1853 1.153977891950802 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 1361 0.8475790129223105 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1350 0.8407286314806165 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 911 0.567336135762105 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 780 0.48575432041102284 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 530 0.3300638330997976 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 471 0.2933208780943484 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 321 0.19990658570761327 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 318 0.19803829985987853 No Hit TAGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA 249 0.15506772536198038 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 247 0.15382220146349057 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 235 0.14634905807255177 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 221 0.13763039078312317 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 218 0.13576210493538846 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 198 0.12330686595049042 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 188 0.11707924645804142 No Hit TAGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA 179 0.11147438891483731 No Hit TAGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACCAAAAAAAAAAAAAAA 166 0.10337848357465358 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 163 0.10151019772691888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 6.227619492449011E-4 0.0 0.0 0.0 0.0 10 0.0037365716954694066 0.0 0.0 0.0 0.0 11 0.011832477035653122 0.0 0.0 0.0 0.0 12 0.014946286781877626 0.0 0.0 0.0 0.0 13 0.019305620426591934 0.0 0.0 0.0 0.0 14 0.024287716020551145 0.0 0.0 0.0 0.0 15 0.02677876381753075 0.0 0.0 0.0 0.0 16 0.031760859411489956 0.0 0.0 0.0 0.0 17 0.03549743110695937 0.0 0.0 0.0 0.0 18 0.03736571695469407 0.0 0.0 0.0 0.0 19 0.03923400280242877 0.0 0.0 0.0 0.0 20 0.05044371788883699 0.0 0.0 0.0 0.0 21 0.0548030515335513 0.0 0.0 0.0 0.0 22 0.061030671026000315 0.0 0.0 0.0 0.0 23 0.07597695780787794 0.0 0.0 0.0 0.0 24 0.08345010119881675 0.0 0.0 0.0 0.0 25 0.08905495874202086 0.0 0.0 0.0 0.0 26 0.09279153043749026 0.0 0.0 0.0 0.0 27 0.09839638798069437 0.0 0.0 0.0 0.0 28 0.10400124552389849 0.0 0.0 0.0 0.0 29 0.10400124552389849 0.0 0.0 0.0 0.0 30 0.107115055270123 0.0 0.0 0.0 0.0 31 0.1089833411178577 0.0 0.0 0.0 0.0 32 0.1096061030671026 0.0 0.0 0.0 0.0 33 0.1096061030671026 0.0 0.0 0.0 0.0 34 0.1096061030671026 0.0 0.0 0.0 0.0 35 0.1096061030671026 0.0 0.0 0.0 0.0 36 0.1096061030671026 0.0 0.0 0.0 0.0 37 0.1102288650163475 0.0 0.0 0.0 0.0 38 0.1102288650163475 0.0 0.0 0.0 0.0 39 0.1102288650163475 0.0 0.0 0.0 0.0 40 0.1102288650163475 0.0 0.0 0.0 0.0 41 0.1114743889148373 0.0 0.0 0.0 0.0 42 0.1145881986610618 0.0 0.0 0.0 0.0 43 0.12268410400124552 0.0 0.0 0.0 0.0 44 0.12766619959520473 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCG 15 0.00800224 50.644264 38 CGGCCAT 15 0.00800224 50.644264 40 GCTTACC 20 3.9173928E-4 50.644264 38 ACTAACA 15 0.00800224 50.644264 38 CCAACGG 15 0.00800224 50.644264 39 ATGCCAT 45 1.1823431E-10 50.644264 40 TCAACAA 15 0.00800224 50.644264 40 GAACCGA 15 0.008052524 50.564102 37 AAATCCG 20 4.003999E-4 50.420456 35 ATATGCG 15 0.00820479 50.32514 34 TTAAGAC 20 4.0415788E-4 50.32514 34 TTGAAAC 15 0.00820479 50.32514 34 ATTCTGA 15 0.00820479 50.32514 34 CGATTTA 15 0.0082971705 50.182846 49 AGATTTA 15 0.0082971705 50.182846 49 CGATCAA 15 0.0082971705 50.182846 49 TCCTATG 15 0.008317805 50.151337 47 ACGATTC 165 0.0 50.151337 48 AACGATT 360 0.0 50.151337 47 CACAAAC 20 4.111202E-4 50.151337 47 >>END_MODULE