FastQCFastQC Report
Fri 17 Jun 2016
SRR1526289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17443
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1170.6707561772630855No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC700.40130711460184604No Hit
TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG370.21211947486097574No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC370.21211947486097574No Hit
TCGTAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC340.19492059852089663No Hit
TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG330.18918763974087024No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC270.15478988706071203No Hit
TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG270.15478988706071203No Hit
TCGTAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG260.14905692828068567No Hit
TCGTAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG240.13759101072063293No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC240.13759101072063293No Hit
TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG230.13185805194060654No Hit
TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG230.13185805194060654No Hit
TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG230.13185805194060654No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC230.13185805194060654No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTCGCCTAGCAAGCGC200.11465917560052744No Hit
TCGTAAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG180.1031932580404747No Hit
TCGTAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG180.1031932580404747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACA354.650974E-850.32267439
TACGCCC150.008083235550.32267438
GCGCTTG150.008083235550.32267438
GCACCCG150.008083235550.32267440
TACCCAC203.94198E-450.32267438
CGCACAG354.650974E-850.32267440
GCACAGT309.4550523E-750.32267441
GACCAGT150.008083235550.32267441
GTACGCC150.008083235550.32267437
GTTCTGA650.050.17681546
AACAATA150.00817692250.1768132
GGGTCCG150.00817692250.1768135
GAAATGG309.647956E-750.1768127
GCAAGCG402.3519533E-950.1768149
TTCCGCA150.00817692250.1768131
GGGTACG505.456968E-1250.1768135
GAAAAAT150.00817692250.1768125
ATGAATT354.7597496E-850.1768125
AGGAATT550.050.1768125
AAATAAC150.00817692250.1768128
Produced by FastQC (version 0.11.5)