##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526289_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17443 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88717537120908 38.0 36.0 39.0 33.0 40.0 2 35.953276385942786 38.0 35.0 39.0 29.0 40.0 3 36.14802499570028 38.0 35.0 39.0 30.0 40.0 4 35.845840738405094 38.0 35.0 39.0 28.0 40.0 5 34.22048959467981 38.0 35.0 39.0 17.0 40.0 6 33.261193602018004 38.0 33.0 39.0 15.0 40.0 7 35.716505188327694 38.0 35.0 39.0 29.0 40.0 8 35.739666341799 38.0 35.0 39.0 29.0 40.0 9 35.68927363412257 38.0 35.0 39.0 28.0 40.0 10 35.39500085994381 38.0 35.0 39.0 27.0 40.0 11 34.5003726423207 38.0 33.0 39.0 26.0 40.0 12 32.190792868199274 36.0 29.0 39.0 18.0 40.0 13 31.536375623459268 35.0 28.0 38.0 16.0 40.0 14 31.09109671501462 35.0 27.0 38.0 14.0 40.0 15 31.92398096657685 36.0 28.0 38.0 17.0 40.0 16 32.55753024135756 36.0 30.0 38.0 19.0 40.0 17 32.644728544401765 36.0 30.0 38.0 19.0 40.0 18 32.59519578054234 36.0 30.0 38.0 20.0 39.0 19 33.51940606547039 36.0 31.0 38.0 25.0 40.0 20 33.71868371266411 36.0 31.0 38.0 25.0 40.0 21 35.232987444820274 38.0 35.0 39.0 28.0 40.0 22 34.96829673794645 38.0 35.0 39.0 27.0 40.0 23 35.122857306655966 38.0 35.0 39.0 27.0 40.0 24 34.734392019721376 38.0 33.0 39.0 26.0 40.0 25 35.00882875652124 38.0 35.0 39.0 27.0 40.0 26 35.29799919738577 38.0 35.0 39.0 28.0 40.0 27 35.25351143725277 38.0 35.0 39.0 28.0 40.0 28 35.12108008943416 38.0 35.0 39.0 28.0 40.0 29 34.94547956200195 38.0 35.0 39.0 27.0 40.0 30 34.80599667488391 38.0 35.0 39.0 27.0 40.0 31 34.39586080376082 38.0 34.0 39.0 25.0 40.0 32 33.42911196468497 38.0 33.0 39.0 19.0 40.0 33 34.68457260792295 38.0 35.0 39.0 27.0 40.0 34 34.38055380381815 38.0 34.0 39.0 26.0 40.0 35 34.396548758814426 38.0 34.0 39.0 27.0 40.0 36 32.390357163332 35.0 31.0 38.0 22.0 39.0 37 32.09264461388523 35.0 31.0 38.0 19.0 39.0 38 31.14143209310325 35.0 30.0 38.0 12.0 39.0 39 29.360029811385655 34.0 27.0 38.0 5.0 39.0 40 25.401364444189646 31.0 12.0 37.0 4.0 39.0 41 21.954193659347588 26.0 9.0 33.0 4.0 37.0 42 25.470962563779167 30.0 19.0 34.0 4.0 37.0 43 21.992833801524966 26.0 8.0 33.0 4.0 37.0 44 22.798601158057675 28.0 10.0 33.0 4.0 37.0 45 25.16958092071318 30.0 19.0 34.0 4.0 37.0 46 25.224445336238034 30.0 15.0 36.0 4.0 38.0 47 24.50908673966634 30.0 10.0 36.0 4.0 38.0 48 24.452731754858682 30.0 10.0 35.0 4.0 38.0 49 22.13696038525483 27.0 5.0 35.0 4.0 38.0 50 24.349251848879206 30.0 4.0 35.0 4.0 38.0 51 25.599610158802957 32.0 4.0 38.0 4.0 39.0 52 25.448661354124862 32.0 4.0 38.0 4.0 39.0 53 25.128762254199394 32.0 4.0 38.0 4.0 39.0 54 24.283953448374707 31.0 4.0 37.0 4.0 39.0 55 24.407326721320874 31.0 4.0 37.0 4.0 39.0 56 23.020008026142293 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.779556436489365 1 2 1.7536329333104206 1 3 0.3276314535178102 1 4 1.4336897407522002 1 5 3.3845159453537477 1 6 5.819023877533887 1 7 2.422171233100407 1 8 2.0233498374194525 1 9 1.4661043771400344 1 10 1.8840244424856252 1 11 -2.3941192016134707 1 12 -4.632957299942351 1 13 -18.69700974479241 1 14 -16.44296392139628 1 15 -10.973056600733308 1 16 -21.4886258492617 1 17 -22.217966472762246 1 18 -21.676607945328875 1 19 -6.889784364071641 1 20 -11.692643998809643 1 21 -13.857239126088889 1 22 -7.889865410108811 1 23 -6.03555039053273 1 24 -6.875447032214478 1 25 -7.562531163079907 1 26 -0.6547795551195037 1 27 -0.7946853761855337 1 28 -0.25598265506950213 1 29 -0.39700883132493203 1 30 -1.252564920303378 1 31 0.06047834348670733 1 32 -8.947571308775952 1 33 -1.0897792048447812 1 34 -1.7316238014049468 1 35 -1.0357273647174239 1 36 1.5861194993623577 1 37 2.1167210393554967 1 38 2.270411719629415 1 39 4.4858738980007935 1 40 6.146340140600508 1 41 -2.506993405375539 1 42 -5.836855088542443 1 43 -14.006230115203891 1 44 -16.073081751805187 1 45 -15.118687118251106 1 46 -17.255249369948846 1 47 -16.787770481777148 1 48 -16.283873872801323 1 49 -13.706305835988083 1 50 -15.923576764881869 1 51 -15.576135215459225 1 52 -17.088290253925187 1 53 -16.775959061326382 1 54 -17.4226392586022 1 55 -16.32566877984106 1 56 -15.829405553877617 2 1 1.381005711851678 2 2 2.364744044421535 2 3 0.9073415984453419 2 4 2.8370713832642807 2 5 4.630892756947951 2 6 5.389072186712632 2 7 2.9728958707815707 2 8 2.1247991127817656 2 9 2.2583749085410005 2 10 2.528951978717508 2 11 -2.316824515623132 2 12 -12.492860681584865 2 13 -15.385415541893861 2 14 -14.986442182265845 2 15 -8.091414088656013 2 16 -4.587659665686825 2 17 -3.1334254099603136 2 18 -12.531680409096992 2 19 -2.491233639433961 2 20 -5.1805667041236525 2 21 2.7587029028966157 2 22 2.5062698555916683 2 23 1.8581694162305524 2 24 2.762234127205808 2 25 1.495439851412847 2 26 2.5481189956051367 2 27 2.574879841205771 2 28 2.0797661372010268 2 29 2.1537158063562316 2 30 3.017966480662807 2 31 -0.1544975019239203 2 32 -5.16254715418658 2 33 0.603457510131058 2 34 0.5147530101892528 2 35 0.35799244204586245 2 36 4.991916600811631 2 37 4.157783841287859 2 38 3.4926339418516363 2 39 7.150125105730265 2 40 10.204311155093261 2 41 3.831170845832194 2 42 -2.1895120933733594 2 43 -3.4699982311459188 2 44 -12.077912669679582 2 45 -4.618687118251106 2 46 -12.390515070431936 2 47 -13.208060336849611 2 48 -10.281458413864124 2 49 -12.725629507485667 2 50 -9.235170967780418 2 51 -10.078550674396423 2 52 -9.588290253925187 2 53 -10.10204601784812 2 54 -15.64486148082442 2 55 -10.91262530158019 2 56 -9.054043235037037 3 1 1.9062231031560302 3 2 2.924744044421537 3 3 2.4742981201844714 3 4 2.409245296307759 3 5 4.917849278687086 3 6 6.361246099756109 3 7 2.6755045664337445 3 8 3.1300165040861145 3 9 3.590548821584477 3 10 3.210691109152286 3 11 0.9614363539420836 3 12 0.011487144502090985 3 13 -6.223676411459078 3 14 -5.702963921396282 3 15 -4.437501045177751 3 16 1.415818595182742 3 17 1.2909224161266408 3 18 1.7100587213377878 3 19 -0.2564510307383081 3 20 -0.3370884432540855 3 21 -0.06390579275555552 3 22 1.292356812113411 3 23 2.6355607205783826 3 24 1.057886301118856 3 25 1.9841355035867636 3 26 2.638553778213833 3 27 0.14531462381446403 3 28 2.288461789374942 3 29 1.296324502008403 3 30 0.6318795241410626 3 31 2.4715894545978188 3 32 0.10353980233515614 3 33 -0.2208903159558986 3 34 0.3617095319283834 3 35 1.3753837463936875 3 36 2.492786166029024 3 37 2.9856099282443793 3 38 1.7726339418516375 3 39 6.421429453556353 3 40 8.693006807267178 3 41 9.088562150180017 3 42 6.900922689235337 3 43 3.5404365514627756 3 44 3.5846960259725904 3 45 7.901312881748893 3 46 1.0425284078289323 3 47 -4.521103815110479 3 48 -0.19276276169021145 3 49 -9.915194724876972 3 50 -0.5569100982152015 3 51 -2.607246326570337 3 52 -3.268290253925187 3 53 -3.2359590613263833 3 54 -5.884861480824421 3 55 -5.065668779841058 3 56 -0.5827388872109509 4 1 1.7526747160592535 4 2 3.138937592808631 4 3 2.7362336040554425 4 4 2.908600135017437 4 5 5.653333149654827 4 6 6.71866545459482 4 7 3.820020695465999 4 8 3.6255003750538606 4 9 4.071839144165125 4 10 2.5990782059264816 4 11 4.709823450716279 4 12 5.037293596114996 4 13 5.620194556282858 4 14 2.798971562474687 4 15 6.114757019338377 4 16 4.681625046795645 4 17 4.009632093545996 4 18 4.570703882628109 4 19 3.362903807971371 4 20 3.3145244599717216 4 21 2.8793200136960593 4 22 3.558163263726314 4 23 2.3658833012235405 4 24 2.4256282366027264 4 25 2.362200019715793 4 26 2.5817795846654477 4 27 3.156927527040274 4 28 1.6884617893749407 4 29 2.1298728891051795 4 30 0.6112343628507446 4 31 0.768363648146206 4 32 3.082894641044838 4 33 1.4926580711408803 4 34 2.4081611448316096 4 35 1.9302224560711068 4 36 4.41407648860967 4 37 4.24754541211535 4 38 5.234569425722604 4 39 6.864010098717639 4 40 7.780748742751047 4 41 5.01630408566389 4 42 8.037696882783724 4 43 8.789468809527293 4 44 10.059534735650011 4 45 9.44582901078115 4 46 7.780592923957965 4 47 6.298251023599196 4 48 6.972398528632368 4 49 2.30803108157464 4 50 6.597928611462219 4 51 3.7966246411716007 4 52 4.637516197687717 4 53 3.3692022289961976 4 54 -0.2900227711470009 4 55 3.4969118653202322 4 56 1.627583693434211 5 1 2.6499268068597317 5 2 4.068447748125237 5 3 3.4387425646289174 5 4 3.1559119629744217 5 5 6.680812241650045 5 6 7.226431284941292 5 7 3.792541603470781 5 8 4.060386874456491 5 9 4.317956228991882 5 10 3.5321725906337704 5 11 5.050325242830972 5 12 5.996672329687275 5 13 7.247434699652036 5 14 6.871850893418536 5 15 6.712128584451875 5 16 6.307670447034596 5 17 6.578329823534048 5 18 6.2863550176340866 5 19 4.499104524817248 5 20 4.7518004456347995 5 21 3.8279460590962984 5 22 3.4434679232245244 5 23 3.482968127985785 5 24 4.013441856674412 5 25 2.8078392072904634 5 26 2.8637389633990153 5 27 2.4090183275181687 5 28 2.5403136412267955 5 29 2.8622504279343275 5 30 3.4141017463632863 5 31 3.4864042694126383 5 32 5.367243506038861 5 33 3.44725783219225 5 34 2.2683761985950497 5 35 1.5383467093566523 5 36 4.28982320306606 5 37 5.135239557874009 5 38 5.196337645555339 5 39 8.504392416519313 5 40 7.775969770230137 5 41 0.8263399279577968 5 42 9.422404170716817 5 43 10.512288403314628 5 44 12.000992322268889 5 45 8.492423992860004 5 46 6.81882470412523 5 47 6.915933221926558 5 48 7.697607608680158 5 49 8.330731201048955 5 50 6.465312124007021 5 51 6.349790710466703 5 52 6.893191227556294 5 53 5.687003901636579 5 54 5.651434815471877 5 55 7.359516405344127 5 56 6.85577963130757 6 1 2.0616077185406425 6 2 3.7878209674984546 6 3 2.5327596586460146 6 4 3.4507837578462173 6 5 5.7563108171486235 6 6 7.310476868986875 6 7 3.870889181818356 6 8 3.9592472733168833 6 9 4.0643949754306306 6 10 2.0122295706907494 6 11 5.31528250778824 6 12 7.311487144502092 6 13 6.174785127002462 6 14 8.441651463219106 6 15 6.774806647129942 6 16 6.378895518259661 6 17 5.2093839545881835 6 18 4.2977510290300955 6 19 4.789702815415538 6 20 4.593680787515147 6 21 3.3607095918598304 6 22 3.33235681211341 6 23 2.2294068744245337 6 24 3.227117070349621 6 25 1.3349047343559945 6 26 2.3323999320599853 6 27 1.089930008429853 6 28 1.9576925586057072 6 29 2.2440168097007103 6 30 2.4226487549102984 6 31 4.056204839213208 6 32 2.471232110027465 6 33 -0.7393518544174356 6 34 1.1401710703899255 6 35 0.8446145156244569 6 36 1.3297092429521022 6 37 1.4671483897828423 6 38 1.0310954803131764 6 39 2.8491217612486572 6 40 -0.37930088504051795 6 41 -1.989899388281522 6 42 6.863999612312259 6 43 7.81428270530893 6 44 7.2431575644341315 6 45 4.381312881748894 6 46 7.214836100136626 6 47 7.2250500310433665 6 48 6.7503141613867115 6 49 8.443266813584568 6 50 6.050782209477106 6 51 6.351215211891205 6 52 5.911709746074813 6 53 4.416348630981311 6 54 6.739753903790962 6 55 5.789715835543557 6 56 6.260338035865974 7 1 2.529556436489365 7 2 3.5730773777548706 7 3 2.95263145351781 7 4 3.6559119629744217 7 5 5.509515945353748 7 6 7.416246099756108 7 7 3.8805045664337428 7 8 3.815016504086117 7 9 3.9522154882511416 7 10 4.1756911091522895 7 11 4.661436353942086 7 12 7.603153811168756 7 13 7.7196569218742574 7 14 9.55703607860372 7 15 7.874165621488913 7 16 6.914151928516077 7 17 7.170922416126643 7 18 6.281725388004453 7 19 4.6102156359283555 7 20 4.8212448900792495 7 21 3.3510942072444436 7 22 4.70735681211341 7 23 4.533894053911716 7 24 4.2078863011188545 7 25 3.0624688369200967 7 26 2.928553778213832 7 27 3.038647957147802 7 28 2.8134617893749407 7 29 3.8113245020084037 7 30 3.941879524141065 7 31 3.1715894545978216 7 32 5.205206469001823 7 33 3.2991096840441045 7 34 3.6017095319283854 7 35 3.8253837463936904 7 36 2.0444528326956863 7 37 2.4222765949110467 7 38 2.700967275184972 7 39 4.583096120223015 7 40 0.06300680726717545 7 41 -9.826437849819982 7 42 6.899256022568668 7 43 11.243769884796109 7 44 10.038029359305924 7 45 6.922979548415562 7 46 10.397528407828933 7 47 10.12889618488952 7 48 11.910570571643124 7 49 12.36313860845636 7 50 10.909756568451463 7 51 11.854420340096329 7 52 13.161709746074813 7 53 12.474040938673618 7 54 13.146805185842243 7 55 13.090997886825605 7 56 12.08726111278905 8 1 2.253915410848336 8 2 3.903205582883075 8 3 3.1096827355690877 8 4 3.5277068347692975 8 5 6.179387740225543 8 6 7.348938407448415 8 7 3.6401199510491296 8 8 3.80540111947073 8 9 4.333625744661397 8 10 3.4353064937676763 8 11 4.584513277019006 8 12 7.119179452194398 8 13 6.790169742387075 8 14 8.55703607860372 8 15 6.544037416360709 8 16 6.725049364413508 8 17 7.093999339203563 8 18 6.451597182876249 8 19 5.405087430800151 8 20 5.2090654028997605 8 21 3.3607095918598304 8 22 4.75543373519033 8 23 4.07556072057838 8 24 4.034809378041935 8 25 3.9118278112790676 8 26 2.640092239752292 8 27 2.7053146238144663 8 28 1.9576925586057072 8 29 2.090170655854557 8 30 2.345725677987218 8 31 1.1331279161362815 8 32 3.586616725412078 8 33 1.9144942994287177 8 34 2.1401710703899255 8 35 2.383076054085997 8 36 0.09894001218286874 8 37 1.4286868513213058 8 38 2.108018557236253 8 39 3.3491217612486572 8 40 -3.263916269655901 8 41 -9.528360926743058 8 42 5.863999612312259 8 43 9.35274424377047 8 44 8.397003718280285 8 45 6.7274667279027405 8 46 8.59945148475201 8 47 8.41735772335106 8 48 9.827237238309788 8 49 10.904805275123028 8 50 11.127705286400186 8 51 12.735830596506585 8 52 11.565555899920966 8 53 11.454810169442851 8 54 13.278215442252499 8 55 12.366638912466634 8 56 11.952645728173664 9 1 2.9027448422864666 9 2 4.190831000943277 9 3 2.9508198593149118 9 4 4.054462687612109 9 5 6.587414496078388 9 6 7.519506969321327 9 7 4.103330653390266 9 8 4.385668677999163 9 9 4.040983604193173 9 10 4.528951978717508 9 11 5.074479832202954 9 12 5.98540018798035 9 13 8.266758371149617 9 14 7.62225346990807 9 15 7.256411998300507 9 16 6.455818595182741 9 17 6.127444155257081 9 18 5.55527611264214 9 19 4.856592447522559 9 20 6.167259382832867 9 21 3.2369637724618343 9 22 2.6801828990699335 9 23 2.8146911553609897 9 24 3.197016735901464 9 25 2.8432659383693704 9 26 0.9394233434312227 9 27 0.40096679772751287 9 28 -0.22458168888592667 9 29 1.240672328095357 9 30 2.017966480662807 9 31 2.410719889380431 9 32 2.141800671900377 9 33 2.7338922927397533 9 34 2.3843182275805574 9 35 0.9666880942197764 9 36 2.296264426898585 9 37 3.4621316673748197 9 38 3.579590463590769 9 39 5.715342497034609 9 40 -3.621775801428477 9 41 -7.168829154167808 9 42 5.54961834140925 9 43 8.35608872537582 9 44 5.400348199885634 9 45 3.6856607078358508 9 46 6.739919712176757 9 47 6.400635315324301 9 48 6.5446285426576125 9 49 8.622196579470852 9 50 6.547437727871756 9 51 8.53014497777749 9 52 7.672579311292203 9 53 7.4196931125866605 9 54 7.050790693088622 9 55 5.783026872332854 9 56 5.511174156267312 10 1 2.1862231031560313 10 2 2.8447440444215317 10 3 2.114298120184472 10 4 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2.191879524141065 115 31 0.028732311740675698 115 32 -3.6936030548076992 115 33 0.6562525411869586 115 34 0.7445666747855313 115 35 2.64681231782226 115 36 2.252786166029022 115 37 1.934181356815813 115 38 2.778348227565921 115 39 3.184286596413493 115 40 5.170149664410033 115 41 3.7628478644657335 115 42 0.44092268923533595 115 43 3.4461508371770613 115 44 -1.3667325454559816 115 45 -2.6186871182511062 115 46 -5.54890016359964 115 47 -1.8353895293961955 115 48 -6.101334190261641 115 49 -8.738051867734114 115 50 -2.685481526786628 115 51 0.3841822448582377 115 52 -1.3025759682109026 115 53 -0.9188162041835248 115 54 -0.5734329093958479 115 55 -0.18281163698391723 115 56 3.1229753985033355 116 1 1.1605088174417446 116 2 2.0076011872786808 116 3 1.7085838344701898 116 4 1.9892452963077574 116 5 3.9321349929727987 116 6 3.1841032426132543 116 7 1.469790280719458 116 8 1.6900165040861168 116 9 2.2676916787273314 116 10 2.4078339662951436 116 11 -1.9099922174864865 116 12 -1.545655712640766 116 13 -6.0779621257447936 116 14 -10.371535349967708 116 15 -1.7746439023206086 116 16 -12.615609976245832 116 17 -13.543363298159072 116 18 -3.438512707233638 116 19 -5.270736745024024 116 20 -3.708517014682659 116 21 -0.5953343641841258 116 22 1.6894996692562643 116 23 1.1469892920069498 116 24 0.9578863011188545 116 25 -0.753007353556093 116 26 0.178553778213832 116 27 -0.7232568047569643 116 28 -0.31153821062505926 116 29 -0.06367549799159633 116 30 -1.450977618716081 116 31 -0.256981973973609 116 32 -6.122174483379126 116 33 -0.27231888738446486 116 34 -0.11257618235732991 116 35 -2.138901967892025 116 36 2.609929023171876 116 37 2.7198956425300977 116 38 4.492633941851636 116 39 5.041429453556351 116 40 8.955863950124321 116 41 4.048562150180018 116 42 0.6552084035210513 116 43 -13.125277734251508 116 44 -1.0810182597416933 116 45 -5.975829975393964 116 46 -10.69175730645678 116 47 -11.978246672253338 116 48 -11.387048475975925 116 49 -12.738051867734114 116 50 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-4.224653890288824 118 53 -4.094140879508199 118 54 -4.463043299006237 118 55 -4.462032416204696 118 56 -5.0263752508473125 119 1 0.529556436489365 119 2 2.114744044421535 119 3 1.0776314535178102 119 4 2.072578629641093 119 5 1.551182612020419 119 6 1.9579127664227727 119 7 0.42217123310040705 119 8 0.2733498374194525 119 9 -1.4227845117488584 119 10 -1.1993088908477105 119 11 -12.671896979391246 119 12 -13.105179522164576 119 13 -4.613676411459078 119 14 -5.942963921396281 119 15 -5.167501045177751 119 16 -8.960848071483927 119 17 -10.829077583873357 119 18 -5.176607945328879 119 19 -3.9731176974049767 119 20 -4.137088443254086 119 21 -2.1905724594222242 119 22 -3.250976521219922 119 23 -2.4244392794216196 119 24 -7.2921136988811455 119 25 -2.145864496413239 119 26 -1.2381128884528323 119 27 -1.461352042852198 119 28 -1.3115382106250593 119 29 -1.8136754979915963 119 30 -1.1414538091922708 119 31 -2.9950772120688463 119 32 -14.50312686433151 119 33 -2.7842236492892276 119 34 -3.3149571347382825 119 35 -2.5079495869396418 119 36 1.6694528326956863 119 37 0.6722765949110503 119 38 2.159300608518304 119 39 2.2914294535563506 119 40 3.8963401406005076 119 41 4.63189548351335 119 42 1.690922689235336 119 43 -0.5062301152038913 119 44 -3.4619706406940765 119 45 -1.285353784917774 119 46 -8.310804925504401 119 47 -13.287770481777146 119 48 -12.006096095023546 119 49 -10.928528058210306 119 50 -7.923576764881867 119 51 -7.270579659903669 119 52 -7.254956920591853 119 53 -10.692625727993049 119 54 -12.39486148082442 119 55 -10.909002113174394 119 56 -7.079405553877615 120 1 1.7462231031560265 120 2 3.164744044421532 120 3 2.2942981201844717 120 4 2.7892452963077545 120 5 5.317849278687085 120 6 5.3412460997561055 120 7 2.9555045664337456 120 8 2.690016504086117 120 9 3.0105488215844787 120 10 0.35069110915228663 120 11 -9.738563646057912 120 12 -4.888512855497908 120 13 -6.663676411459079 120 14 -4.942963921396281 120 15 -8.917501045177751 120 16 -11.14418140481726 120 17 -12.329077583873357 120 18 -6.009941278662211 120 19 -5.456451030738307 120 20 -6.737088443254088 120 21 -4.423905792755555 120 22 -3.367643187886589 120 23 -4.124439279421619 120 24 -8.742113698881145 120 25 -3.9958644964132404 120 26 -3.5214462217861673 120 27 -3.0946853761855344 120 28 -3.11153821062506 120 29 -2.863675497991597 120 30 -3.7081204758589337 120 31 -5.92841054540218 120 32 -11.936460197664843 120 33 -4.500890315955896 120 34 -7.598290468071614 120 35 -5.42461625360631 120 36 -2.0472138339709787 120 37 -1.094390071755619 120 38 -0.20736605814836295 120 39 0.24142945355634993 120 40 -4.986993192732825 120 41 -4.2514378498199825 120 42 -7.859077310764665 120 43 -1.0395634485372227 120 44 -6.095303974027409 120 45 -10.118687118251106 120 46 -15.377471592171068 120 47 -14.82110381511048 120 48 -13.672762761690212 120 49 -14.495194724876972 120 50 -13.156910098215201 120 51 -13.687246326570335 120 52 -13.688290253925187 120 53 -13.475959061326382 120 54 -16.44486148082442 120 55 -12.92566877984106 120 56 -10.362738887210948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 186.0 5 22.0 6 12.0 7 23.0 8 16.0 9 20.0 10 23.0 11 22.0 12 30.0 13 30.0 14 47.0 15 78.0 16 97.0 17 145.0 18 182.0 19 185.0 20 254.0 21 313.0 22 402.0 23 423.0 24 495.0 25 561.0 26 574.0 27 609.0 28 641.0 29 644.0 30 701.0 31 849.0 32 995.0 33 1219.0 34 1459.0 35 1683.0 36 1853.0 37 1600.0 38 877.0 39 173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.58149400905808 0.38984119704179326 0.022931835120105486 0.005732958780026371 8 99.70761910221866 0.18918763974087024 0.04586367024021097 0.05732958780026372 9 99.604198933058 0.26960362530832327 0.07457121551081282 0.05162622612287042 10 65.28120162816029 22.014561715301266 4.873014963022416 7.8312216935160235 11 41.29450209252995 25.225018632116036 17.766439259301727 15.714040016052286 12 41.51808748495098 11.442985724932637 18.37413288998452 28.66479390013186 13 28.38961187869059 16.16121080089434 21.77377744654016 33.67539987387491 14 25.3454899936923 13.670508630082 28.269969608349104 32.7140317678766 15 21.235632183908045 19.310344827586206 29.712643678160923 29.74137931034483 16 30.559219959850875 14.161170060223688 28.391167192429023 26.888442787496412 17 36.26354722174437 17.523940592923907 21.658351969723036 24.554160215608693 18 36.04151138122814 20.514878734017543 21.60999942663838 21.833610458115935 19 32.13691875465856 24.964164898801673 19.024138524167192 23.874777822372568 20 27.348319761440532 24.148411515082007 20.403716022479642 28.09955270099782 21 27.78510406513388 19.144544464193565 29.26437704260077 23.805974428071785 22 25.839926613920422 19.550510262584567 29.406031418415317 25.20353170507969 23 28.91704408645302 22.524795046723614 26.348678553001204 22.209482313822164 24 27.60419652582698 26.21108754228057 21.383936249498365 24.800779682394083 25 29.52408256880734 26.370412844036693 20.11467889908257 23.990825688073393 26 25.498419994254522 20.43665613329503 25.389255960930768 28.675667911519675 27 28.000458741900335 16.061700785595505 28.063535753196856 27.874304719307304 28 22.973516401447693 23.07117826161889 24.943988050784167 29.01131728614925 29 25.018640665328363 24.754803556065387 26.64181244622885 23.584743332377403 30 26.82381280110117 17.74489561826107 33.02936453314981 22.40192704748796 31 22.99246996608611 17.76168304880152 37.328274989940795 21.91757199517158 32 35.23913292808808 14.502809955270099 27.669457506594792 22.588599610047027 33 31.46222349899454 18.71301350186728 29.342143062338412 20.48261993679977 34 24.995694356736898 15.20179114759745 37.2753889431081 22.527125552557553 35 28.536179491593504 21.415045618867275 34.30883112411775 15.739943765421472 36 29.52090992647059 20.43313419117647 29.36580882352941 20.68014705882353 37 27.896946345390766 22.226695037092416 29.817700845362012 20.058657772154813 38 29.315792497271215 20.646866203251566 32.87757798586776 17.159763313609467 39 23.18590186592951 19.235199262842663 26.503109882515552 31.075788988712276 40 17.287372250129916 12.858710087187482 22.166406836422425 47.68751082626018 41 20.087968053706813 14.39319405058163 24.092829446148503 41.42600844956305 42 21.21996090605956 19.834425664022078 34.511900655398414 24.43371277451995 43 12.90879778903731 12.563334868724091 25.875172731460154 48.65269461077844 44 16.477239946922058 12.42139271909075 24.450470201350026 46.65089713263716 45 23.560149296583404 19.40855584266437 32.50645994832041 24.52483491243181 46 25.358741820686486 17.403283205142923 32.15474687177132 25.083228102399264 47 18.392980041293875 17.60724936912136 36.4762560220234 27.52351456756137 48 21.119972459693614 20.276550576625166 38.407252280681625 20.1962246829996 49 17.107989868754316 12.894312687082662 23.929311535804743 46.06838590835828 50 22.28867165037378 16.802760207015528 37.998849913743534 22.909718228867167 51 26.400000000000002 22.58992805755396 31.712230215827336 19.297841726618707 52 20.892343120322394 26.41335636154289 30.88658606793322 21.807714450201495 53 26.093453646761304 18.76544629001667 34.95603195585954 20.18506810736249 54 19.93340222758066 17.763233436674707 33.585945573544606 28.717418762200026 55 25.19820751465012 17.982304952315292 32.80477996093301 24.014707572101575 56 26.36933157077993 19.04707167078568 29.53618024024369 25.0474165181907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 4.0 13 16.5 14 26.0 15 32.5 16 39.0 17 54.5 18 70.0 19 75.5 20 81.0 21 100.0 22 136.0 23 153.0 24 204.5 25 256.0 26 296.0 27 336.0 28 367.0 29 427.5 30 457.0 31 429.0 32 401.0 33 362.5 34 324.0 35 278.0 36 232.0 37 210.0 38 201.5 39 215.0 40 229.5 41 244.0 42 254.0 43 264.0 44 292.5 45 321.0 46 405.5 47 567.0 48 644.0 49 630.5 50 617.0 51 635.0 52 653.0 53 693.0 54 812.0 55 891.0 56 992.0 57 1093.0 58 1264.5 59 1436.0 60 1376.5 61 1317.0 62 1230.5 63 1108.5 64 1073.0 65 979.5 66 886.0 67 813.5 68 741.0 69 666.0 70 591.0 71 491.5 72 323.5 73 255.0 74 212.0 75 169.0 76 129.0 77 89.0 78 73.0 79 42.0 80 27.0 81 18.0 82 9.0 83 5.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.05732958780026372 10 0.0 11 0.0 12 0.0 13 0.0 14 0.022931835120105486 15 0.24651722754113398 16 0.04586367024021097 17 0.022931835120105486 18 0.011465917560052743 19 0.011465917560052743 20 0.02866479390013186 21 0.011465917560052743 22 0.005732958780026371 23 0.0 24 0.0 25 0.017198876340079116 26 0.21785243364100212 27 0.022931835120105486 28 0.2063865160809494 29 0.04586367024021097 30 0.040130711460184605 31 0.2637161038812131 32 0.02866479390013186 33 0.21785243364100212 34 0.13759101072063293 35 0.09172734048042194 36 0.20065355730092302 37 0.30957977412142407 38 0.2063865160809494 39 0.45290374362208335 40 0.71088688872327 41 0.9402052399243249 42 0.2809149802212922 43 0.4299719085019779 44 0.6306254658029009 45 0.16052284584073842 46 0.1203921343805538 47 0.040130711460184605 48 0.08026142292036921 49 0.40704007338187237 50 0.30384681534139774 51 0.38984119704179326 52 0.41850599094192514 53 0.25225018632116036 54 0.1433239695006593 55 0.21211947486097574 56 0.25225018632116036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 17443.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.48741615547785 #Duplication Level Percentage of deduplicated Percentage of total 1 93.88837332626277 81.20162816029352 2 3.81810950550179 6.604368514590381 3 1.19978788280525 3.1129966175543196 4 0.35794776614079277 1.2383190964856963 5 0.16571655839851518 0.7166198475032964 6 0.0795439480312873 0.4127730321618988 7 0.07291528569534668 0.4414378260620306 8 0.05965796102346547 0.4127730321618988 9 0.06628662335940606 0.5159662902023734 >10 0.27840381810950554 4.271054291119647 >50 0.0066286623359406065 0.40130711460184604 >100 0.0066286623359406065 0.6707561772630855 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 117 0.6707561772630855 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 70 0.40130711460184604 No Hit TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 37 0.21211947486097574 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 37 0.21211947486097574 No Hit TCGTAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 34 0.19492059852089663 No Hit TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 33 0.18918763974087024 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 27 0.15478988706071203 No Hit TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 27 0.15478988706071203 No Hit TCGTAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 26 0.14905692828068567 No Hit TCGTAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 24 0.13759101072063293 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 24 0.13759101072063293 No Hit TCGTAAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 23 0.13185805194060654 No Hit TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 23 0.13185805194060654 No Hit TCGTAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 23 0.13185805194060654 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 23 0.13185805194060654 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTCGCCTAGCAAGCGC 20 0.11465917560052744 No Hit TCGTAAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 18 0.1031932580404747 No Hit TCGTAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 18 0.1031932580404747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011465917560052743 0.0 0.0 0.0 0.0 11 0.011465917560052743 0.0 0.0 0.0 0.0 12 0.011465917560052743 0.0 0.0 0.0 0.0 13 0.017198876340079116 0.0 0.0 0.0 0.0 14 0.022931835120105486 0.0 0.0 0.0 0.0 15 0.022931835120105486 0.0 0.0 0.0 0.0 16 0.022931835120105486 0.0 0.0 0.0 0.0 17 0.022931835120105486 0.0 0.0 0.0 0.0 18 0.022931835120105486 0.0 0.0 0.0 0.0 19 0.022931835120105486 0.0 0.0 0.0 0.0 20 0.022931835120105486 0.0 0.0 0.0 0.0 21 0.022931835120105486 0.0 0.0 0.0 0.0 22 0.022931835120105486 0.0 0.0 0.0 0.0 23 0.022931835120105486 0.0 0.0 0.0 0.0 24 0.022931835120105486 0.0 0.0 0.0 0.0 25 0.022931835120105486 0.0 0.0 0.0 0.0 26 0.022931835120105486 0.0 0.0 0.0 0.0 27 0.022931835120105486 0.0 0.0 0.0 0.0 28 0.022931835120105486 0.0 0.0 0.0 0.0 29 0.022931835120105486 0.0 0.0 0.0 0.0 30 0.02866479390013186 0.0 0.0 0.0 0.0 31 0.02866479390013186 0.0 0.0 0.0 0.0 32 0.02866479390013186 0.0 0.0 0.0 0.0 33 0.02866479390013186 0.0 0.0 0.0 0.0 34 0.02866479390013186 0.0 0.0 0.0 0.0 35 0.02866479390013186 0.0 0.0 0.0 0.0 36 0.02866479390013186 0.0 0.0 0.0 0.0 37 0.03439775268015823 0.0 0.0 0.0 0.0 38 0.03439775268015823 0.0 0.0 0.0 0.0 39 0.03439775268015823 0.0 0.0 0.0 0.0 40 0.0401307114601846 0.0 0.0 0.0 0.0 41 0.0401307114601846 0.0 0.0 0.0 0.0 42 0.0401307114601846 0.0 0.0 0.0 0.0 43 0.0401307114601846 0.0 0.0 0.0 0.0 44 0.04586367024021097 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCACA 35 4.650974E-8 50.322674 39 TACGCCC 15 0.0080832355 50.322674 38 GCGCTTG 15 0.0080832355 50.322674 38 GCACCCG 15 0.0080832355 50.322674 40 TACCCAC 20 3.94198E-4 50.322674 38 CGCACAG 35 4.650974E-8 50.322674 40 GCACAGT 30 9.4550523E-7 50.322674 41 GACCAGT 15 0.0080832355 50.322674 41 GTACGCC 15 0.0080832355 50.322674 37 GTTCTGA 65 0.0 50.176815 46 AACAATA 15 0.008176922 50.17681 32 GGGTCCG 15 0.008176922 50.17681 35 GAAATGG 30 9.647956E-7 50.17681 27 GCAAGCG 40 2.3519533E-9 50.17681 49 TTCCGCA 15 0.008176922 50.17681 31 GGGTACG 50 5.456968E-12 50.17681 35 GAAAAAT 15 0.008176922 50.17681 25 ATGAATT 35 4.7597496E-8 50.17681 25 AGGAATT 55 0.0 50.17681 25 AAATAAC 15 0.008176922 50.17681 28 >>END_MODULE