FastQCFastQC Report
Fri 17 Jun 2016
SRR1526288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116854
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC2490.21308641552706797No Hit
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG2340.2002498844712205No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG2200.18826912215242952No Hit
AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2160.18484604720420353No Hit
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2080.17799989730775156No Hit
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG2060.17628835983363858No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1940.1660191349889606No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1680.14376914782549163No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1420.12151916066202269No Hit
AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGAGCCACGCAGGGATGAGA1380.11809608571379669No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1370.1172403169767402No Hit
AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1340.11467301076557071No Hit
AATAAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG1320.11296147329145773No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1290.11039416708028822No Hit
AATAAAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1260.10782686086911873No Hit
AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGACCCACGCAGGGATGAGA1210.10354801718383624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAACC750.050.50065238
ACCGGTG150.00808705550.5006539
TACACAC354.8388756E-850.5006538
CGGCGAA309.764117E-750.45683337
GTACGAC150.00811486850.45683337
AGATCCC150.00818471150.3476236
AAAAGTA150.00821277450.30406635
TTAAGAC150.00828324350.1955134
GCCTTGA252.0387839E-550.1522242
TCGCCTA150.00831155750.15221842
GCCTTCA150.00831155750.15221842
ACCAGCT150.00831155750.15221842
AGTTATG204.1236955E-450.1090145
TTCTCAA204.1236955E-450.1090145
TCGCAAC150.008339941550.10900548
CTCCATC150.008339941550.10900549
CTGAATC150.008339941550.10900549
ACGGATG252.0492173E-550.10900548
GCATATT150.008339941550.10900549
CACGGAT402.6066118E-950.0874347