##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526288_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 116854 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.90727745733993 39.0 38.0 39.0 35.0 40.0 2 37.757064370924404 39.0 38.0 39.0 35.0 40.0 3 37.07156793948003 39.0 37.0 39.0 32.0 40.0 4 36.644462320502505 39.0 36.0 39.0 31.0 40.0 5 36.929185137008574 39.0 37.0 39.0 33.0 40.0 6 33.05563352559604 38.0 32.0 39.0 14.0 40.0 7 34.43850445855512 38.0 35.0 39.0 25.0 40.0 8 35.597651770585514 38.0 35.0 39.0 28.0 40.0 9 35.61662416348606 38.0 35.0 39.0 28.0 40.0 10 35.3536464305886 38.0 35.0 39.0 27.0 40.0 11 33.90614784260701 38.0 33.0 39.0 23.0 40.0 12 31.682124702620364 36.0 28.0 39.0 14.0 40.0 13 30.99623461755695 35.0 27.0 39.0 12.0 40.0 14 30.742096975713284 36.0 26.0 39.0 11.0 40.0 15 31.280298492135486 36.0 27.0 39.0 13.0 40.0 16 31.667653653276737 36.0 28.0 39.0 14.0 40.0 17 31.85902065825731 36.0 29.0 38.0 15.0 40.0 18 31.878566416211683 36.0 29.0 38.0 17.0 40.0 19 32.86373594399849 36.0 30.0 38.0 22.0 40.0 20 33.05868006229996 36.0 30.0 39.0 22.0 40.0 21 34.62029541136803 38.0 35.0 39.0 25.0 40.0 22 34.34425008985572 38.0 34.0 39.0 23.0 40.0 23 34.54768343402879 38.0 34.0 39.0 25.0 40.0 24 34.24285860988926 38.0 33.0 39.0 23.0 40.0 25 34.45372002669998 38.0 34.0 39.0 25.0 40.0 26 34.89420986872508 38.0 35.0 39.0 27.0 40.0 27 34.84068153422219 38.0 35.0 39.0 26.0 40.0 28 34.73339380765742 38.0 35.0 39.0 27.0 40.0 29 34.702329402502265 38.0 35.0 39.0 26.0 40.0 30 34.51121057045544 38.0 35.0 39.0 25.0 40.0 31 34.320682218837185 38.0 35.0 39.0 24.0 40.0 32 33.14467626268677 38.0 33.0 39.0 15.0 40.0 33 34.324969620209835 38.0 35.0 39.0 25.0 40.0 34 34.09986821161449 38.0 34.0 39.0 23.0 40.0 35 34.10160542215072 38.0 34.0 39.0 24.0 40.0 36 31.922835332979616 35.0 31.0 38.0 16.0 39.0 37 31.71571362554983 35.0 31.0 38.0 11.0 39.0 38 30.88685025758639 35.0 30.0 38.0 4.0 39.0 39 29.15133414346107 34.0 27.0 38.0 4.0 39.0 40 25.19766546288531 31.0 11.0 37.0 4.0 39.0 41 21.494069522652197 25.0 8.0 33.0 4.0 37.0 42 25.322710390744007 30.0 19.0 34.0 4.0 37.0 43 21.823745870915843 26.0 7.0 33.0 4.0 37.0 44 22.784200797576464 28.0 9.0 33.0 4.0 37.0 45 25.114262241771783 30.0 18.0 34.0 4.0 38.0 46 25.359987676930185 31.0 12.0 36.0 4.0 38.0 47 24.53257911581974 30.0 6.0 36.0 4.0 38.0 48 24.502515960086946 30.0 7.0 36.0 4.0 38.0 49 21.98546904684478 27.0 4.0 35.0 4.0 38.0 50 24.382699779211666 30.0 4.0 35.0 4.0 38.0 51 25.71435295325791 32.0 4.0 38.0 4.0 40.0 52 25.550481797798962 32.0 4.0 38.0 4.0 40.0 53 25.200917384086125 32.0 4.0 38.0 4.0 40.0 54 24.238845054512467 31.0 4.0 38.0 4.0 39.0 55 24.493487599911 31.0 4.0 38.0 4.0 39.0 56 23.695183733547847 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.19491703884187928 1 2 0.18256747823750175 1 3 1.4947841743436712 1 4 1.720781911596923 1 5 1.5264131469153241 1 6 5.811634167091373 1 7 3.638796973962336 1 8 3.2718939538638168 1 9 3.1244241004969666 1 10 2.993213705003974 1 11 -2.174723931844383 1 12 -5.320262816870763 1 13 -19.42367706508967 1 14 -16.570151467195778 1 15 -9.810125701554472 1 16 -20.169303747224035 1 17 -20.1117129369052 1 18 -19.363346587529016 1 19 -5.324439171497225 1 20 -9.736257640140142 1 21 -6.1329262442366606 1 22 -1.7100866288416903 1 23 -2.240967860834097 1 24 -2.5572403853811316 1 25 -2.4257970615240403 1 26 2.7973264309878303 1 27 2.7605418568385076 1 28 2.894225161595969 1 29 2.646441150439884 1 30 2.6220152587214116 1 31 1.822574155504249 1 32 -9.724491572461652 1 33 1.1886495531457513 1 34 1.4941192123417721 1 35 1.6883090480901117 1 36 4.028194897766646 1 37 4.256441243575246 1 38 4.738618567256513 1 39 6.901155465723107 1 40 9.552745458214229 1 41 -2.411908429246374 1 42 -6.773122424659441 1 43 -13.618540428183739 1 44 -15.641024683526487 1 45 -14.269979261506457 1 46 -18.176137060270364 1 47 -17.479723859571386 1 48 -16.309594035655273 1 49 -14.282900581520675 1 50 -16.046776018846607 1 51 -17.443988706434684 1 52 -17.24054630036899 1 53 -17.033130703977182 1 54 -17.41914475531585 1 55 -16.484409900422502 1 56 -15.379303664278485 2 1 0.06648857331185809 2 2 0.5609032825369837 2 3 1.6645395486950463 2 4 1.8378130457703818 2 5 1.7977252299397861 2 6 6.020492580731105 2 7 3.816521214140245 2 8 3.499377274842317 2 9 3.2916776216237267 2 10 3.2913048836548313 2 11 -1.6215363855137674 2 12 -9.287646063720281 2 13 -12.296267873095601 2 14 -14.577935010561236 2 15 -5.244521550331346 2 16 -2.4197670533767415 2 17 -1.9144371770831086 2 18 -9.401245030820345 2 19 -1.5280158949961162 2 20 -3.456420723905893 2 21 2.714460709062081 2 22 3.2067962473629095 2 23 2.545633325229659 2 24 2.681640266953927 2 25 2.745440892515994 2 26 2.178812717125709 2 27 2.4046300703299863 2 28 2.4617751986604617 2 29 2.544513796844626 2 30 2.8055771564234178 2 31 -2.5306504553185825 2 32 -10.121730267791527 2 33 -2.3771399205384576 2 34 -1.7445761175322048 2 35 -2.1845597436074407 2 36 3.4359969733782094 2 37 3.26941381585101 2 38 4.085727536863626 2 39 6.121040565797237 2 40 8.557285858510742 2 41 6.102083416565339 2 42 2.5922408073642806 2 43 -0.1746931338916724 2 44 -8.802903853281862 2 45 1.6061326732600385 2 46 -11.085792360492752 2 47 -10.704334682403116 2 48 -7.821269350703458 2 49 -11.646132605241949 2 50 -8.71430752366499 2 51 -8.309444599688947 2 52 -8.743696782207387 2 53 -8.49977266098237 2 54 -14.847980930260249 2 55 -8.516933992342445 2 56 -7.5510049244712185 3 1 0.8140085512672215 3 2 1.1777743213906575 3 3 2.450982094851014 3 4 2.7493933709288285 3 5 2.326796599463073 3 6 7.007748023001707 3 7 4.699891288540904 3 8 4.043776558391507 3 9 3.87610170539336 3 10 3.839906426779656 3 11 3.6342503325903905 3 12 4.622108689655306 3 13 -2.24360184939761 3 14 -3.097361849910918 3 15 -0.7883352731199942 3 16 4.056638292448554 3 17 4.117768664746599 3 18 3.8611206346522344 3 19 2.3204616470264803 3 20 2.135064533740838 3 21 4.218559634385862 3 22 4.9228681680022035 3 23 4.218511528591463 3 24 4.289452336394547 3 25 4.4791140807221055 3 26 3.8514153624825624 3 27 3.487331916568614 3 28 3.5366925312589714 3 29 3.8536898784097957 3 30 3.7336220723782247 3 31 4.116910856337526 3 32 4.20711376637767 3 33 4.047288296601252 3 34 4.628253863178735 3 35 4.603949487585723 3 36 6.63202204915666 3 37 6.812263085622298 3 38 7.103156580677354 3 39 9.5245608193106 3 40 11.323853783558963 3 41 11.808097470023593 3 42 9.462074094771278 3 43 4.418514361286064 3 44 4.996317695354126 3 45 9.109749372075136 3 46 3.1185683449357384 3 47 -1.0618711938387726 3 48 2.8721034792772535 3 49 -8.136488075068348 3 50 1.9973210241443233 3 51 0.8591968993465997 3 52 0.844550668881066 3 53 0.1074194029470874 3 54 -4.6322190123765346 3 55 -0.03584416043282701 3 56 2.978376447807644 4 1 0.48926900925018657 4 2 1.1029686732865827 4 3 2.112439601858256 4 4 2.3360187873971725 4 5 2.0725790311093775 4 6 7.040827508960771 4 7 4.8179832024443385 4 8 3.972064675161299 4 9 3.914152369098474 4 10 3.6524824487797645 4 11 4.8852419238961176 4 12 4.970461325966056 4 13 6.932134572412107 4 14 4.645882107992723 4 15 7.392107902087581 4 16 7.212542888143723 4 17 6.704050329562293 4 18 6.344435458281193 4 19 5.0337198838449275 4 20 5.481909951266886 4 21 4.227153425702163 4 22 4.499260362195443 4 23 4.244663096627846 4 24 4.546472802839098 4 25 3.750757213621789 4 26 3.0676351520007756 4 27 3.460783712009942 4 28 3.091692795683322 4 29 3.854721133367754 4 30 3.0444793361150673 4 31 3.5222469396840808 4 32 4.432429753473759 4 33 3.1937000581962565 4 34 3.5821118144188873 4 35 3.456056949640832 4 36 5.07628139655737 4 37 5.51423040277286 4 38 5.662149652759428 4 39 7.4989645424054245 4 40 9.287416108377837 4 41 5.842181768505796 4 42 8.212721934424355 4 43 8.163291980409234 4 44 10.810533148313034 4 45 8.183285783307884 4 46 8.495082174329152 4 47 8.430434057628784 4 48 8.032133094804252 4 49 5.923245385188672 4 50 8.747149148317998 4 51 6.7243140393328495 4 52 7.0459098100307855 4 53 6.630477206109603 4 54 0.9585049814888187 4 55 6.838395619566114 4 56 6.825988695943455 5 1 0.8195485488320529 5 2 1.2611786803337992 5 3 2.037856562158936 5 4 2.571748174289354 5 5 2.1701242507476195 5 6 7.19674717069439 5 7 4.261135657224699 5 8 3.718012525760315 5 9 3.5271427379027998 5 10 3.5393158995667022 5 11 5.570936074706552 5 12 7.626674621714237 5 13 8.222244978801587 5 14 7.973166580626032 5 15 7.869615536571956 5 16 7.102723766696698 5 17 7.3996693100649935 5 18 7.1640854465358395 5 19 5.65910160806386 5 20 5.610286742434376 5 21 4.20084381799721 5 22 4.70909122900305 5 23 4.170021330125614 5 24 4.453978088251361 5 25 4.423531467791385 5 26 3.51219705005105 5 27 3.5378920042345143 5 28 3.201218283115786 5 29 3.649470957187347 5 30 3.852853778210438 5 31 3.6303042570437185 5 32 4.9258523515475225 5 33 3.6537658322155195 5 34 3.733392058722387 5 35 3.8160522514966004 5 36 4.725623656364746 5 37 5.1222289933295855 5 38 5.443744672726542 5 39 7.408525265919636 5 40 5.803154891558478 5 41 2.99146530151026 5 42 9.707142888147633 5 43 12.843054724125466 5 44 12.040272401308101 5 45 9.506224472525645 5 46 10.290125632163306 5 47 10.436424191525894 5 48 10.973406291891404 5 49 11.81806568117176 5 50 10.392791619541864 5 51 9.69177939051913 5 52 9.291798933826886 5 53 9.537865037916038 5 54 9.112545385529224 5 55 9.034014600068815 5 56 8.805023227303568 6 1 1.2978605425608265 6 2 1.6669795687581512 6 3 2.835740021787892 6 4 3.1490933532807333 6 5 2.850356808887156 6 6 7.732349470145653 6 7 5.4671717168013885 6 8 4.510539309141549 6 9 4.453194475556309 6 10 4.161278272182329 6 11 6.048333513954006 6 12 9.08787138157836 6 13 8.928672990298839 6 14 9.335134179267268 6 15 8.667956273441568 6 16 8.553991077906524 6 17 7.7228336685703525 6 18 7.353855501409104 6 19 6.43849800210619 6 20 6.595915172013683 6 21 4.752973423432273 6 22 5.462488145647946 6 23 4.776044324638292 6 24 5.200711822246653 6 25 4.888255794940591 6 26 4.300412362306076 6 27 4.355177083931402 6 28 4.700761005875123 6 29 4.608381330848175 6 30 4.5481111599716115 6 31 4.855807262008447 6 32 6.0835476742546 6 33 4.486402362134513 6 34 4.8648266027516485 6 35 4.257782065972691 6 36 4.935775211107362 6 37 4.695197375874663 6 38 5.796697377194793 6 39 6.668128087973464 6 40 4.75546740788981 6 41 2.026610323720867 6 42 8.457926791988765 6 43 12.639174400111877 6 44 11.28380519461879 6 45 8.282877203124023 6 46 11.567888893203286 6 47 11.455629835633552 6 48 11.202828816061771 6 49 12.4431636691519 6 50 10.942700164093733 6 51 10.77748621048255 6 52 10.577858510518812 6 53 10.72672053830799 6 54 11.452433025978664 6 55 10.542572217001137 6 56 10.608589989780704 7 1 0.9732391453942384 7 2 1.698489094893965 7 3 2.750065864484519 7 4 2.9876986292944068 7 5 2.741661156713242 7 6 7.624325532676185 7 7 5.023901076840474 7 8 4.20246651969358 7 9 3.797112404232422 7 10 3.998112663975192 7 11 5.472857207163578 7 12 7.937468352755694 7 13 8.697725261915839 7 14 9.005016934518853 7 15 8.343579136167513 7 16 7.443219216646142 7 17 7.264052525434053 7 18 7.047553596181945 7 19 6.4931511530588075 7 20 5.973785225750859 7 21 4.44300761981723 7 22 5.161315698163818 7 23 4.516457123856661 7 24 5.105267269242262 7 25 4.241150274667021 7 26 3.9339614585444735 7 27 3.8582913975611035 7 28 3.9612031162806005 7 29 4.216710593183301 7 30 4.2197647994440075 7 31 4.751386157953732 7 32 5.812480258524264 7 33 4.650606074884884 7 34 4.313776285213791 7 35 4.27939906646121 7 36 2.724612534018945 7 37 3.132972168253751 7 38 3.4146749052846808 7 39 5.758626378154219 7 40 -0.17497958771351207 7 41 -8.853580198995303 7 42 6.0727900064489475 7 43 9.818155836360045 7 44 8.815803240539648 7 45 5.954530842596423 7 46 8.8873197676537 7 47 8.881191633386358 7 48 9.134680306047112 7 49 11.35358135356812 7 50 9.070875542696566 7 51 10.04782084653531 7 52 10.214827245252568 7 53 10.414128940848297 7 54 10.954936689877051 7 55 10.70818042413353 7 56 9.914098050357158 8 1 1.1693939581612653 8 2 1.355815056190771 8 3 2.672753725756195 8 4 2.829712954501744 8 5 2.636483976517212 8 6 7.14408552262341 8 7 5.05176611830818 8 8 4.070680895164806 8 9 4.215810569600606 8 10 3.9340262100923127 8 11 5.7799695469474415 8 12 8.041985819455867 8 13 8.723925312179727 8 14 8.91674098578802 8 15 8.430409998193142 8 16 8.375677120642123 8 17 7.657398843604447 8 18 7.775625976176915 8 19 6.174258215134703 8 20 6.459460018412329 8 21 4.701112671337711 8 22 5.099365459410365 8 23 4.75178635230602 8 24 5.025190616817028 8 25 4.622684579768801 8 26 3.5924497721911237 8 27 3.7917231643366804 8 28 3.4227198290224905 8 29 3.5393419075839034 8 30 3.7716936760461692 8 31 3.9512479322688847 8 32 5.524185460836403 8 33 3.882291171642862 8 34 4.06132592080062 8 35 4.120125379605213 8 36 2.0256303776982634 8 37 2.4102717410107317 8 38 2.700802072914737 8 39 4.394276038872988 8 40 -1.0123445035215042 8 41 -9.23275838446904 8 42 5.9977397425635495 8 43 9.737765034651073 8 44 8.500146040238064 8 45 5.948656250990489 8 46 8.91964572895051 8 47 9.642029783675376 8 48 8.95687181145048 8 49 11.297128727280107 8 50 8.94084915415673 8 51 9.860619288354862 8 52 9.712722445051508 8 53 9.658882647809843 8 54 9.738267928881825 8 55 9.653015809908847 8 56 9.683669550734134 9 1 0.8367802663999981 9 2 1.350139173469131 9 3 2.4774755030387325 9 4 2.615317801635946 9 5 2.6093929534654663 9 6 7.1404989218852 9 7 4.630566870449691 9 8 3.7959054929780223 9 9 3.690774007165892 9 10 3.769982753027051 9 11 5.583124362742417 9 12 8.168282915353913 9 13 8.14547277370212 9 14 8.825996504974594 9 15 8.441274264506959 9 16 7.33570219202592 9 17 7.306155720824314 9 18 7.237705508177751 9 19 5.702881378307627 9 20 5.481118908307167 9 21 4.247355445904184 9 22 4.810870438865763 9 23 4.098502697455402 9 24 4.725552759027487 9 25 3.9673812856675426 9 26 3.3838697875125234 9 27 3.5892369187764004 9 28 2.671588134614801 9 29 3.5248562191654926 9 30 3.926613935117139 9 31 4.039102239671905 9 32 5.3712151982832985 9 33 4.387444733868641 9 34 4.369564656088592 9 35 4.462872088986089 9 36 3.759316567544353 9 37 3.796997836142701 9 38 3.2399761101039566 9 39 4.973529468777578 9 40 1.0591089403116314 9 41 -3.226943216281839 9 42 6.865940870775823 9 43 10.929143264332804 9 44 9.977930008480978 9 45 7.366199255377982 9 46 10.094692737795139 9 47 10.373661512904427 9 48 10.359929127419559 9 49 12.9610328988798 9 50 10.369395710323598 9 51 9.509515451040286 9 52 9.298313363639156 9 53 9.811260947134304 9 54 9.898689963842475 9 55 9.645320287936567 9 56 10.106017903683504 10 1 0.7488649688461493 10 2 1.136302006619914 10 3 2.2447628341814863 10 4 2.4238975752759657 10 5 2.26247802100837 10 6 6.874160842294106 10 7 4.409902394363527 10 8 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5.753935302639341 114 40 8.38491743745811 114 41 6.832346574460075 114 42 2.075135544206386 114 43 0.10162265558201256 114 44 -0.7384301690713357 114 45 1.0785010943126672 114 46 -3.539739728913805 114 47 -8.362229419245221 114 48 -3.7186377717560894 114 49 -13.325079973663001 114 50 -3.1090443657702522 114 51 -3.6357603891626304 114 52 -2.9200125716810703 114 53 -4.220825292561319 114 54 -4.61640198289183 114 55 -2.973512939710865 114 56 0.021363496581411567 115 1 -0.3528478111275035 115 2 -0.018902209453862895 115 3 1.0598484731801676 115 4 0.9061768901639766 115 5 0.2851394175828048 115 6 3.455847271806622 115 7 1.931509772492646 115 8 2.03398825882401 115 9 2.1340689259715546 115 10 1.606808316149106 115 11 0.23372677238096884 115 12 -4.002749038548657 115 13 -0.5087964772145916 115 14 -5.0710700220028855 115 15 -1.7433773855716197 115 16 -9.198085131179944 115 17 -8.4152953006529 115 18 -1.800272490774578 115 19 -2.0992401512890204 115 20 -2.4936060785969687 115 21 0.44300761981723014 115 22 1.4439243938159976 115 23 0.5218919064653562 115 24 1.3498324866335665 115 25 1.040063318145279 115 26 0.8524397194140434 115 27 1.3745957453871895 115 28 1.8035944206284213 115 29 1.3036671149224333 115 30 1.1165039298787889 115 31 0.8383426796928575 115 32 -5.703824089301822 115 33 0.8951712922761885 115 34 1.1181241113007445 115 35 1.4533121099394677 115 36 4.2463516644537265 115 37 4.388406950862446 115 38 5.414674905284681 115 39 6.802104639023781 115 40 8.613063890547355 115 41 7.548593714048177 115 42 3.121703049927209 115 43 1.2692427928817835 115 44 0.6527597622787802 115 45 3.3186612773790287 115 46 -1.835506319302823 115 47 -5.852504018787556 115 48 -3.995754476561583 115 49 -7.412722994257967 115 50 -2.4780375007816957 115 51 -0.5989182838994722 115 52 -0.04060753735612721 115 53 -1.4608710591517067 115 54 -1.507019831862081 115 55 -1.1505152280403834 115 56 1.202141528618025 116 1 0.11850529736498316 116 2 0.43701002134662303 116 3 1.688149976634051 116 4 1.7417112257998966 116 5 1.2896091453357528 116 6 4.161048409555502 116 7 2.311233462049735 116 8 2.3838460402136903 116 9 2.3769579954555056 116 10 1.861582798067026 116 11 0.4440071462127477 116 12 0.09233426092310282 116 13 -3.8935994028545764 116 14 -8.442666933827347 116 15 -2.5708865281965707 116 16 -7.386626374576938 116 17 -9.461719518450543 116 18 -2.9552907760244764 116 19 -3.218805368680325 116 20 -2.819185111510418 116 21 1.7006264739415684 116 22 2.2452246782532157 116 23 1.7312580178022117 116 24 2.09617543665388 116 25 1.8115992791367503 116 26 2.2183478868256614 116 27 1.9740471878902426 116 28 2.231266098808028 116 29 2.1578930393433993 116 30 2.690660776689029 116 31 0.4993544635205751 116 32 -9.160650582597231 116 33 0.4783691793139724 116 34 0.4005296375096066 116 35 0.2150959376517818 116 36 2.617440652669899 116 37 2.607829949643431 116 38 3.2885066809856163 116 39 4.924717398064821 116 40 6.731105337113792 116 41 4.836281645783242 116 42 1.2981557114469169 116 43 -10.540285854009722 116 44 -1.308130119882943 116 45 -1.308218039971642 116 46 -5.793551829258124 116 47 -8.162032665679062 116 48 -8.326311160836267 116 49 -5.911503977598073 116 50 -5.082974518254268 116 51 -2.211067816609752 116 52 -2.7108635308547058 116 53 -3.0027239644747468 116 54 -6.936520035031524 116 55 -3.869430303213079 116 56 -2.6938357164043225 117 1 -0.6402464846720832 117 2 -0.19723677098665604 117 3 0.31574383058621436 117 4 0.19965515103353937 117 5 -0.14964319111284397 117 6 1.340519342550909 117 7 0.4978325433106292 117 8 0.7804878903641779 117 9 0.9039657572169446 117 10 0.31572504422575065 117 11 -6.876442719144453 117 12 -8.795674609661404 117 13 -7.936209889152693 117 14 -8.127444377198167 117 15 -5.418027348725637 117 16 -9.625498543118045 117 17 -7.182612913033154 117 18 -2.9885554679300697 117 19 -2.6933816398667645 117 20 -2.4996856659219517 117 21 0.3236266323448618 117 22 0.7180585131969863 117 23 -0.32022305300406373 117 24 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3.173782054415316 118 7 1.6527054246665571 118 8 1.8046404327370595 118 9 1.8541776216237267 118 10 1.574199620496934 118 11 -4.503773227619032 118 12 -5.649488168983439 118 13 -7.04955734677981 118 14 -0.37596132635070845 118 15 -14.450442602962925 118 16 -5.388846000745161 118 17 -9.700621387609424 118 18 -1.9894029255571866 118 19 -2.2313053686803244 118 20 -2.795236513379578 118 21 1.8098554459041836 118 22 2.276533089468167 118 23 1.3403701673349175 118 24 -2.1137544698881747 118 25 1.8020198398844087 118 26 1.845917980283609 118 27 2.0756827019089314 118 28 1.7834857249762521 118 29 2.0596453757919946 118 30 2.4496561037918383 118 31 0.3160600709972101 118 32 -11.162519741475737 118 33 0.6136495531457484 118 34 0.41595019825727064 118 35 0.5582034142872985 118 36 3.0142864470624247 118 37 3.241124342166799 118 38 4.427174905284684 118 39 5.318408986849867 118 40 8.53425954272127 118 41 7.2382676270916555 118 42 2.3678987021011224 118 43 -0.9470615549443018 118 44 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2.9673465517121755 119 27 2.493539844766076 119 28 2.778128582119109 119 29 2.568573947220564 119 30 3.2264418180775465 119 31 0.1535600709972087 119 32 -9.205376884332878 119 33 1.8029352674314651 119 34 1.794521626828697 119 35 2.049274842858722 119 36 4.826786447062425 119 37 4.896481485023941 119 38 5.971817762427538 119 39 8.014837558278437 119 40 9.89318811414984 119 41 9.743624769948799 119 42 5.437541559243979 119 43 1.713652730769983 119 44 1.4984119361918218 119 45 2.4897792898013904 119 46 -4.231469052222074 119 47 -9.193808366613641 119 48 -8.658487395816241 119 49 -7.061170199226913 119 50 -4.778969177800327 119 51 -7.198918283899472 119 52 -7.531290767169793 119 53 -10.283231307598912 119 54 -9.923168900185063 119 55 -10.893061811891316 119 56 -7.374877104922346 120 1 -0.03524441770225906 120 2 -0.23258195494697276 120 3 1.3750658644845188 120 4 1.2362405168872002 120 5 1.0210885162042302 120 6 3.3179893714884905 120 7 1.7963029856421713 120 8 1.9369575059077917 120 9 1.715458109428603 120 10 1.8973703522042484 120 11 -5.0589561544483 120 12 0.19502402613851189 120 13 -1.9260817370237113 120 14 -1.3982174239116851 120 15 -4.478796261499511 120 16 -8.7617118544037 120 17 -8.915560411999667 120 18 -2.5881834133620636 120 19 -2.035878539412032 120 20 -3.312004806062504 120 21 0.18637983614808462 120 22 1.0192160162974346 120 23 0.12024821611540659 120 24 -4.409181299156469 120 25 0.10872715695758473 120 26 1.6764057851616556 120 27 1.8409266043479562 120 28 1.618546700586002 120 29 2.036169766035897 120 30 1.7511804940357365 120 31 -2.3586350509540104 120 32 -10.257641692695248 120 33 -2.3723260566103477 120 34 -8.765757118815902 120 35 -0.95734536620051 120 36 0.5414205934038847 120 37 0.9170389763131404 120 38 0.2195529540651684 120 39 1.5531650844108427 120 40 -0.40019167679092504 120 41 1.103206651481898 120 42 -4.165942761313509 120 43 -1.0671835061638149 120 44 -2.58451489307647 120 45 -10.636004682324046 120 46 -12.388263477309184 120 47 -12.627954708077057 120 48 -11.533400287802303 120 49 -14.535734659157225 120 50 -10.86642562379336 120 51 -10.879406088777522 120 52 -10.571360453580944 120 53 -11.928527474846298 120 54 -12.161844858373215 120 55 -11.96414195126414 120 56 -8.286723794817817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 489.0 5 451.0 6 2123.0 7 506.0 8 454.0 9 380.0 10 119.0 11 175.0 12 182.0 13 239.0 14 332.0 15 457.0 16 625.0 17 883.0 18 1037.0 19 1291.0 20 1379.0 21 1742.0 22 2129.0 23 2565.0 24 2937.0 25 3364.0 26 3414.0 27 3847.0 28 4064.0 29 4206.0 30 4948.0 31 5504.0 32 6571.0 33 7550.0 34 8980.0 35 10630.0 36 12155.0 37 11948.0 38 7049.0 39 2129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.17846201242575 0.7119995892310063 0.04877881801222038 0.060759580331011344 8 99.14251972546938 0.6692111523781813 0.10440378592089274 0.08386533623153679 9 98.04581171999384 1.247153886977214 0.33126187663705 0.37577251639189907 10 39.458640696938055 42.72083112259743 7.6308898283327915 10.18963835213172 11 27.869203186910052 30.398877221808597 24.75246677449445 16.979452816786903 12 40.23225563523714 14.924606774265323 19.987334622691563 24.855802967805978 13 27.320416930528697 18.553066219384874 22.066852653738852 32.05966419634758 14 27.716432985807955 19.075377056477155 22.99744919795251 30.210740759762384 15 23.73959485293487 23.294670335793704 22.578847654073332 30.38688715719809 16 26.605088344062455 18.559101492946173 29.293932338035884 25.541877824955485 17 34.864811660689675 23.628645035391184 18.76631546513519 22.740227838783948 18 34.13039385173647 25.924721428204645 19.132019923660202 20.812864796398678 19 23.605987726700846 33.81148417908404 19.367676888710108 23.21485120550501 20 23.24167922202438 28.133132447609917 20.364504862347623 28.260683468018076 21 22.245615836906566 26.037093143673886 28.822074443046535 22.89521657637302 22 24.43582963210187 25.378039074734925 25.8281772825687 24.357954010594508 23 25.92209081417838 31.290328101733788 23.080938607150802 19.706642476937034 24 21.781025895561985 33.15162510483167 21.727112465127423 23.340236534478922 25 22.082634443569642 33.88902201074864 21.58371273063362 22.444630815048093 26 22.990535628557712 28.773746656607912 28.020197517317058 20.215520197517318 27 26.856575478481187 24.361454445852022 27.21265450063341 21.569315575033382 28 20.01834421985633 31.08573779766497 29.37389634658575 19.52202163589295 29 19.53413915901931 29.983050267086703 31.490549239830163 18.992261334063826 30 18.694997089340138 24.87073245899394 38.36763346231552 18.06663698935041 31 18.80119040472045 24.539661575142155 40.02607226477071 16.633075755366683 32 28.061556369612106 21.13903248998596 33.47341572802903 17.3259954123729 33 21.811306517582295 29.48560747022457 34.31312862814367 14.38995738404946 34 19.235941582080603 24.424172341427898 38.258597798620926 18.081288277870573 35 24.337074995932976 26.208762511451884 37.64780423484284 11.806358257772299 36 21.383938903031705 26.477924348787575 36.34275330641913 15.795383441761595 37 22.819056768858616 26.076103552985984 36.0110827085728 15.093756969582598 38 20.542004490992305 28.01899244073433 40.01954095887828 11.419462109395088 39 17.475085910652922 27.59278350515464 32.04639175257732 22.88573883161512 40 11.322558145542533 17.616312612480733 25.173295674712175 45.88783356726455 41 16.00283205401625 20.01087923189169 27.418254660369374 36.568034053722684 42 13.762199619217508 29.170168607742575 36.90416973979863 20.163462033241284 43 9.178030433139835 18.37838766186927 28.463229485109743 43.98035241988115 44 10.571671841270113 18.183069156209957 25.984536392799708 45.260722609720226 45 16.030259674614257 30.300797614865964 36.88476135808709 16.784181352432682 46 19.923433993936385 26.08382864288039 37.80339494013463 16.189342423048593 47 13.947456277767792 24.2884169256187 39.527637244579125 22.236489552034378 48 15.988869863013699 28.198630136986303 41.80993150684931 14.002568493150685 49 12.515989457689106 17.899607668071734 25.638934436784766 43.945468437454394 50 16.03231505463028 26.82029467762131 42.60904615701274 14.538344110735665 51 20.842455932479886 29.156256171170007 36.27488859696572 13.726399299384385 52 13.839373920812323 34.46442224264864 36.34231618373466 15.353887652804385 53 20.25363126575549 29.00639651535678 37.3990362354878 13.340935983399929 54 13.868394217503448 26.182335449925876 36.31884281515377 23.630427517416898 55 16.530937644658565 28.28192775215595 36.20450220309633 18.98263240008915 56 20.2869098454837 29.534736156128343 34.51064121692305 15.667712781464903 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 16.5 2 29.0 3 41.0 4 45.5 5 38.0 6 81.0 7 124.0 8 196.5 9 269.0 10 371.5 11 474.0 12 580.5 13 825.5 14 964.0 15 1133.5 16 1303.0 17 1558.0 18 1813.0 19 2155.5 20 2498.0 21 3061.5 22 4162.5 23 4700.0 24 5059.5 25 5419.0 26 5602.0 27 5785.0 28 5578.0 29 4902.5 30 4434.0 31 4060.0 32 3686.0 33 3314.5 34 2943.0 35 2827.5 36 2712.0 37 2690.0 38 2602.0 39 2536.0 40 2656.5 41 2777.0 42 3196.5 43 3616.0 44 4001.0 45 4386.0 46 4367.0 47 4269.0 48 4190.0 49 4384.5 50 4579.0 51 5006.5 52 5434.0 53 5705.5 54 5931.5 55 5886.0 56 5563.0 57 5240.0 58 4870.5 59 4501.0 60 4263.5 61 4026.0 62 3480.5 63 2698.5 64 2462.0 65 2060.5 66 1659.0 67 1379.0 68 1099.0 69 931.0 70 763.0 71 614.5 72 335.0 73 204.0 74 155.5 75 107.0 76 72.5 77 38.0 78 28.0 79 12.5 80 7.0 81 4.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02396152463758194 10 0.0 11 8.557687370564979E-4 12 0.0 13 0.0 14 0.02396152463758194 15 0.17543259109658205 16 0.03251921200814692 17 0.014548068529960463 18 0.006846149896451983 19 0.012836531055847469 20 0.03251921200814692 21 0.011124993581734473 22 8.557687370564979E-4 23 0.0 24 0.0 25 0.0017115374741129958 26 0.17628835983363858 27 0.022249987163468946 28 0.16773067246307358 29 0.03251921200814692 30 0.03594228695637291 31 0.21822102794940693 32 0.01540383726701696 33 0.19768257826005103 34 0.09327879233915826 35 0.05220189296044637 36 0.1600287538295651 37 0.23619217142759338 38 0.15061529772194363 39 0.38851900662365 40 0.6187207968918479 41 0.8874321803275883 42 0.21565372173823746 43 0.34401903229671216 44 0.4972016362298252 45 0.1121057045544012 46 0.0787307238091978 47 0.03166344327109042 48 0.04621151180105088 49 0.3174902014479607 50 0.21565372173823746 51 0.32947096376675167 52 0.38252862546425453 53 0.19511527204888152 54 0.13435569171787015 55 0.17115374741129957 56 0.19853834699710748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 116854.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.64494155099526 #Duplication Level Percentage of deduplicated Percentage of total 1 93.75094782177918 79.35543498724904 2 4.077402917774565 6.902630633097711 3 0.9766355612621448 2.4800177999897306 4 0.42159112737713705 1.4274222534102383 5 0.20321298945516678 0.8600475807417804 6 0.12637623722336241 0.6418265527923733 7 0.07885877202737815 0.46724973043284784 8 0.06167160376500085 0.41761514368357094 9 0.05156150478713187 0.3927978503089325 >10 0.21433409833082265 3.309257706197477 >50 0.01819817816016419 1.122768583018125 >100 0.019209188057951087 2.622931179078166 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AATAAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 249 0.21308641552706797 No Hit AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 234 0.2002498844712205 No Hit AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 220 0.18826912215242952 No Hit AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 216 0.18484604720420353 No Hit AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 208 0.17799989730775156 No Hit AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 206 0.17628835983363858 No Hit AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 194 0.1660191349889606 No Hit AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 168 0.14376914782549163 No Hit AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 142 0.12151916066202269 No Hit AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGAGCCACGCAGGGATGAGA 138 0.11809608571379669 No Hit AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 137 0.1172403169767402 No Hit AATAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 134 0.11467301076557071 No Hit AATAAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG 132 0.11296147329145773 No Hit AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 129 0.11039416708028822 No Hit AATAAAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 126 0.10782686086911873 No Hit AATAAAGGGATTGACGGGTACGCACAGTTCTGAGTCGGACCCACGCAGGGATGAGA 121 0.10354801718383624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.012836531055847467 0.0 0.0 0.0 0.0 7 0.012836531055847467 0.0 0.0 0.0 0.0 8 0.012836531055847467 0.0 0.0 0.0 0.0 9 0.012836531055847467 0.0 0.0 0.0 0.0 10 0.03251921200814692 0.0 0.0 0.0 0.0 11 0.04877881801222038 0.0 0.0 0.0 0.0 12 0.053913430434559366 0.0 0.0 0.0 0.0 13 0.06247111780512434 0.0 0.0 0.0 0.0 14 0.06931726770157633 0.0 0.0 0.0 0.0 15 0.07359611138685881 0.0 0.0 0.0 0.0 16 0.0795864925462543 0.0 0.0 0.0 0.0 17 0.08643264244270628 0.0 0.0 0.0 0.0 18 0.09156725486504527 0.0 0.0 0.0 0.0 19 0.09926917349855376 0.0 0.0 0.0 0.0 20 0.10697109213206223 0.0 0.0 0.0 0.0 21 0.10953839834323173 0.0 0.0 0.0 0.0 22 0.11124993581734471 0.0 0.0 0.0 0.0 23 0.1206633919249662 0.0 0.0 0.0 0.0 24 0.1223749293990792 0.0 0.0 0.0 0.0 25 0.13178838550670066 0.0 0.0 0.0 0.0 26 0.14633645403666112 0.0 0.0 0.0 0.0 27 0.15403837267016962 0.0 0.0 0.0 0.0 28 0.1625960600407346 0.0 0.0 0.0 0.0 29 0.1660191349889606 0.0 0.0 0.0 0.0 30 0.16944220993718656 0.0 0.0 0.0 0.0 31 0.17029797867424307 0.0 0.0 0.0 0.0 32 0.17200951614835608 0.0 0.0 0.0 0.0 33 0.17200951614835608 0.0 0.0 0.0 0.0 34 0.17200951614835608 0.0 0.0 0.0 0.0 35 0.17200951614835608 0.0 0.0 0.0 0.0 36 0.17286528488541256 0.0 0.0 0.0 0.0 37 0.17286528488541256 0.0 0.0 0.0 0.0 38 0.17372105362246906 0.0 0.0 0.0 0.0 39 0.17372105362246906 0.0 0.0 0.0 0.0 40 0.17372105362246906 0.0 0.0 0.0 0.0 41 0.17457682235952557 0.0 0.0 0.0 0.0 42 0.17971143478186455 0.0 0.0 0.0 0.0 43 0.18399027846714705 0.0 0.0 0.0 0.0 44 0.18655758467831654 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCAACC 75 0.0 50.500652 38 ACCGGTG 15 0.008087055 50.50065 39 TACACAC 35 4.8388756E-8 50.50065 38 CGGCGAA 30 9.764117E-7 50.456833 37 GTACGAC 15 0.008114868 50.456833 37 AGATCCC 15 0.008184711 50.34762 36 AAAAGTA 15 0.008212774 50.304066 35 TTAAGAC 15 0.008283243 50.19551 34 GCCTTGA 25 2.0387839E-5 50.15222 42 TCGCCTA 15 0.008311557 50.152218 42 GCCTTCA 15 0.008311557 50.152218 42 ACCAGCT 15 0.008311557 50.152218 42 AGTTATG 20 4.1236955E-4 50.10901 45 TTCTCAA 20 4.1236955E-4 50.10901 45 TCGCAAC 15 0.0083399415 50.109005 48 CTCCATC 15 0.0083399415 50.109005 49 CTGAATC 15 0.0083399415 50.109005 49 ACGGATG 25 2.0492173E-5 50.109005 48 GCATATT 15 0.0083399415 50.109005 49 CACGGAT 40 2.6066118E-9 50.08743 47 >>END_MODULE