FastQCFastQC Report
Fri 17 Jun 2016
SRR1526287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32636
Sequences flagged as poor quality0
Sequence length56
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG3281.0050251256281406No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG3250.9958328226498345No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG3190.9774482166932222No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2880.8824610859173918No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG2770.848755974996936No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG2430.7445765412427994No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1830.560730481676676No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1780.5454099767128325No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1720.5270253707562201No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1700.5208971687706827No Hit
ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1480.45348694692977076No Hit
ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1430.4381664419659272No Hit
ATTCCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG1280.3922049270743964No Hit
ATTCCAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG1190.36462801813947787No Hit
ATTCCAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG670.20529476651550432No Hit
ATTCCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG670.20529476651550432No Hit
ATTCCAGGGATGCGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG500.15320504963843606No Hit
ATTCCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG420.12869224169628632No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACACACAGTTCTGAGTCG420.12869224169628632No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA390.11949993871798015No Hit
ATTCCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG390.11949993871798015No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACTCACAGTTCTGAGTCG380.11643583772521142No Hit
ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTAACCACAGTTCTGAGTCG380.11643583772521142No Hit
ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG360.11030763573967398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACC309.4206916E-750.55140338
GCCCAGC451.1459633E-1050.55140341
CAACCTG150.008002466550.55140341
CAACCAA150.008002466550.55140340
ACCAACC150.008002466550.55140338
CAACAAA251.913756E-550.55140340
TACGCCC2300.050.55140338
TACGTCC150.008002466550.55140338
CCTTAAA150.008002466550.55140340
TCCGCCC950.050.55140338
GACCAGC150.008002466550.55140341
CGACCAG251.913756E-550.55140340
CGTCCAG150.008002466550.55140340
CCAACAA203.9057387E-450.551439
ACGCCAA203.9057387E-450.551439
AGCCATA203.9057387E-450.551438
CGCCAAG203.9057387E-450.551440
TACGACC203.9057387E-450.551438
CATAGAA203.9057387E-450.551441
CCATAGA203.9057387E-450.551440