##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526287_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32636 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.01623973526167 39.0 37.0 40.0 33.0 40.0 2 36.25321730604241 39.0 37.0 40.0 30.0 40.0 3 36.28321485476161 39.0 37.0 40.0 30.0 40.0 4 35.83383380316215 39.0 36.0 40.0 27.0 40.0 5 36.310117661478124 39.0 37.0 40.0 30.0 40.0 6 35.11946929770805 38.0 35.0 39.0 24.0 40.0 7 35.7950422845937 38.0 36.0 39.0 29.0 40.0 8 35.73501654614536 38.0 35.0 39.0 29.0 40.0 9 35.75805858561098 38.0 36.0 39.0 29.0 40.0 10 35.44512195121951 38.0 35.0 39.0 28.0 40.0 11 34.15715773991911 38.0 33.0 39.0 24.0 40.0 12 31.98293295747028 36.0 29.0 39.0 14.0 40.0 13 31.519028067165095 36.0 28.0 39.0 13.0 40.0 14 30.645177105037384 36.0 25.0 39.0 11.0 40.0 15 31.511306532663315 36.0 28.0 39.0 14.0 40.0 16 31.628293908567226 36.0 28.0 39.0 14.0 40.0 17 32.18709400661846 36.0 29.0 39.0 16.0 40.0 18 32.22560975609756 36.0 29.0 39.0 17.0 40.0 19 33.315510479225395 36.0 31.0 39.0 23.0 40.0 20 33.06796176001961 36.0 30.0 39.0 22.0 40.0 21 34.777362421865426 38.0 35.0 39.0 25.0 40.0 22 34.57399803897536 38.0 34.0 39.0 24.0 40.0 23 34.70789925235936 38.0 34.0 39.0 26.0 40.0 24 34.45511092045594 38.0 34.0 39.0 24.0 40.0 25 34.68458144380439 38.0 35.0 39.0 26.0 40.0 26 34.7901703640152 38.0 35.0 39.0 26.0 40.0 27 34.88923274911141 38.0 35.0 39.0 27.0 40.0 28 34.586193160926584 38.0 34.0 39.0 27.0 40.0 29 34.93580708420149 38.0 35.0 39.0 27.0 40.0 30 34.25444294643952 38.0 34.0 39.0 24.0 40.0 31 34.46261796788822 38.0 35.0 39.0 25.0 40.0 32 33.092014952812846 38.0 33.0 39.0 16.0 40.0 33 34.31642970952323 38.0 35.0 39.0 25.0 40.0 34 34.1966540017159 38.0 34.0 39.0 23.0 40.0 35 34.27344037259468 38.0 35.0 39.0 24.0 40.0 36 32.14968133349675 35.0 31.0 38.0 19.0 39.0 37 31.955693099644563 35.0 31.0 38.0 17.0 39.0 38 30.546972668219144 34.0 29.0 38.0 10.0 39.0 39 29.772337296237286 35.0 28.0 38.0 4.0 39.0 40 25.96289373697757 32.0 12.0 38.0 4.0 39.0 41 21.399528128447113 24.0 9.0 32.0 4.0 37.0 42 25.339379825959064 30.0 20.0 33.0 4.0 37.0 43 22.189912979531805 27.0 8.0 33.0 4.0 36.0 44 23.045134207623484 28.0 10.0 33.0 4.0 36.0 45 25.320382399803897 30.0 20.0 34.0 4.0 37.0 46 25.963414634146343 31.0 18.0 36.0 4.0 38.0 47 24.866129427625935 30.0 10.0 35.0 4.0 38.0 48 25.303468562323815 31.0 14.0 36.0 4.0 38.0 49 22.578900600563795 28.0 5.0 35.0 4.0 38.0 50 25.200085794827796 31.0 12.0 36.0 4.0 38.0 51 26.312170609143276 33.0 4.0 38.0 4.0 40.0 52 26.160221840911877 32.0 4.0 38.0 4.0 40.0 53 25.91984311802917 32.0 4.0 38.0 4.0 40.0 54 24.89321608040201 32.0 4.0 38.0 4.0 39.0 55 25.187155288638312 32.0 4.0 38.0 4.0 40.0 56 25.223587449442334 33.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.723114305707682 1 2 2.410014016701588 1 3 2.9241564983266457 1 4 2.997577692551282 1 5 2.4809829686526896 1 6 3.8150644492049963 1 7 2.88022051635636 1 8 3.1376497029162067 1 9 3.061813344410986 1 10 2.8392272374694585 1 11 -3.0591402338304974 1 12 -6.176124709264663 1 13 -20.214387398816818 1 14 -17.17948233413602 1 15 -12.502100822463163 1 16 -20.14177569100532 1 17 -20.134532402925128 1 18 -18.470581030785674 1 19 -6.674562424555386 1 20 -9.77452253238295 1 21 -6.608586589558488 1 22 -2.596660263153769 1 23 -3.4390755631341676 1 24 -3.3648163183213384 1 25 -4.103179710078884 1 26 1.7337978122558653 1 27 2.461611170527071 1 28 1.1858526715164501 1 29 1.265491625555974 1 30 2.269103245241162 1 31 0.7899966747109275 1 32 -9.487880376551672 1 33 1.544647329529738 1 34 0.7941770517366749 1 35 1.9083537201450511 1 36 2.8809816784128124 1 37 3.203061485596674 1 38 2.839327926206302 1 39 5.116389188811542 1 40 7.487981234567783 1 41 -3.8181985039591595 1 42 -8.971312260401463 1 43 -13.900567172186069 1 44 -16.63651955185889 1 45 -15.477481589160178 1 46 -18.81957314682619 1 47 -18.0793526993578 1 48 -17.44304227328267 1 49 -15.451548452119757 1 50 -17.470674207372237 1 51 -17.780544951951782 1 52 -17.6847919404223 1 53 -17.296881248667077 1 54 -18.07204636551686 1 55 -17.508409966204557 1 56 -16.691627804299607 2 1 1.1295795945259144 2 2 2.535610201121301 2 3 3.0296148979026896 2 4 2.4660461610197544 2 5 2.5694832336235365 2 6 3.968217602358145 2 7 2.9199661443372804 2 8 2.752912024060876 2 9 2.7973724329112457 2 10 2.9409760451006193 2 11 -1.0220443143816347 2 12 -8.661021370631914 2 13 -11.079252263681681 2 14 -12.267982599106872 2 15 -4.743224298880758 2 16 -1.4131058446884204 2 17 -0.5123808395970926 2 18 -8.769998094908619 2 19 -0.6655534155463769 2 20 -1.7103995859070622 2 21 3.25892798383844 2 22 2.4579237326066945 2 23 2.5974904146082807 2 24 2.8365615301153326 2 25 3.0399045506524374 2 26 3.151391876908754 2 27 2.659279427018852 2 28 3.1641250615535483 2 29 3.228395706107115 2 30 3.268043361828333 2 31 0.051787877678606264 2 32 -5.768219539243777 2 33 0.175277960160372 2 34 0.8291532043598906 2 35 0.8421110068435169 2 36 4.545528578253833 2 37 4.820973515273405 2 38 5.817070374536989 2 39 7.255763857597973 2 40 9.403190561541813 2 41 9.39695782884423 2 42 5.339233579556144 2 43 -0.8449232930127799 2 44 -8.309545519002501 2 45 1.7170070170931346 2 46 -9.277442781166412 2 47 -8.70097432097942 2 48 -6.93959765219099 2 49 -10.924256454239524 2 50 -6.574542781829049 2 51 -6.275510505740868 2 52 -6.726127393522461 2 53 -7.029790628635283 2 54 -14.474802062390207 2 55 -5.599559939707472 2 56 -5.628564741236545 3 1 1.7387299213232978 3 2 2.786590593278163 3 3 3.1956279697981174 3 4 3.1549350499086444 3 5 2.7224244100941277 3 6 4.168217602358148 3 7 2.940881177017019 3 8 3.498010063276567 3 9 3.4588103414079825 3 10 3.486074084316307 3 11 3.4315504568601938 3 12 4.030481897341943 3 13 -5.034807819237237 3 14 -2.4431459977996823 3 15 -3.3915903119526547 3 16 3.311077161847532 3 17 3.369972101579375 3 18 2.9005901403854963 3 19 0.6455576955647366 3 20 1.565417407556989 3 21 4.137359356387456 3 22 4.251387784894277 3 23 3.9465100224514202 3 24 3.997345843840826 3 25 3.741865334966164 3 26 3.578842857300913 3 27 3.1991487080646053 3 28 3.876543362207144 3 29 3.6636898237541686 3 30 3.9033374794753968 3 31 3.9119185966328516 3 32 3.4226301339588403 3 33 3.864166849049262 3 34 4.442225099784729 3 35 2.7035489153402494 3 36 6.971672369103505 3 37 6.644502927038115 3 38 6.958246845125224 3 39 9.070142942565294 3 40 11.64894219552874 3 41 12.543363057602399 3 42 9.242501553412353 3 43 3.1799133082944095 3 44 5.146663631324294 3 45 9.229425317746728 3 46 3.502949375696332 3 47 -1.7009743209794195 3 48 1.6734742432338479 3 49 -9.227524428095734 3 50 0.2842807475827165 3 51 -1.2376020090088424 3 52 -1.3274345830649459 3 53 -0.9461304979163287 3 54 -7.929704023174521 3 55 -1.8531547109493012 3 56 1.3936574809856772 4 1 1.8882248708182487 4 2 2.853257259944833 4 3 3.2420926162627595 4 4 3.5145310095046014 4 5 3.061818349488071 4 6 4.665187299327847 4 7 3.314618550754396 4 8 3.510131275397775 4 9 3.0790123616099976 4 10 3.4981952964375225 4 11 4.003267628577369 4 12 5.131491998352047 4 13 5.320747736318317 4 14 5.185136830483149 4 15 5.9013389809766394 4 16 7.3009761517465215 4 17 6.957850889458168 4 18 6.2581658979612556 4 19 4.655658705665747 4 20 5.587639629779215 4 21 4.500995720023823 4 22 3.9645190980255904 4 23 3.934388810330205 4 24 4.379164025659001 4 25 3.657016850117678 4 26 2.5849034633615204 4 27 3.4738961828120836 4 28 2.502805988469767 4 29 2.657629217693561 4 30 3.6912162673541786 4 31 3.328080212794468 4 32 4.061013972342678 4 33 1.714671899554311 4 34 3.7371745947342205 4 35 2.8995085112998424 4 36 3.7797531771843147 4 37 3.7657150482502324 4 38 4.740065026943405 4 39 6.235799508221863 4 40 8.52975027633682 4 41 2.5110398252791697 4 42 6.458663169573967 4 43 5.874862803243907 4 44 8.041613126273791 4 45 7.292051580372991 4 46 6.115070587817545 4 47 6.008116588111491 4 48 4.871454041213646 4 49 1.2654048648335596 4 50 5.69640195970393 4 51 4.3462363748295445 4 52 4.668525012894651 4 53 4.348818997033167 4 54 0.18544749197699417 4 55 5.466037208242618 4 56 5.074465561793758 5 1 2.642584796606748 5 2 4.143053178312179 5 3 4.318530464129182 5 4 4.316386297074175 5 5 4.0176625053322255 5 6 5.240326445895562 5 7 4.076028569307269 5 8 4.307533872800377 5 9 4.006656146396644 5 10 3.8833529958809336 5 11 5.198443880896477 5 12 8.312568065142393 5 13 7.492176307746888 5 14 8.732817721021181 5 15 8.05829630936254 5 16 8.543276708332787 5 17 8.315550332871897 5 18 8.224399664195019 5 19 6.674582638875396 5 20 7.244782486922066 5 21 5.541440989040524 5 22 5.469528374463437 5 23 4.785965804764345 5 24 5.042243803024498 5 25 4.703770096870926 5 26 4.520339455940366 5 27 4.640872064073676 5 28 4.014865357671994 5 29 4.681376898584105 5 30 4.836670812808727 5 31 5.029378914093172 5 32 6.052108592008722 5 33 4.678679320704589 5 34 4.737916709762054 5 35 5.411938938015986 5 36 6.240606609466315 5 37 6.107087961051715 5 38 6.003144804308896 5 39 8.69553976796212 5 40 8.097468272626251 5 41 1.169666912477684 5 42 11.894655748423688 5 43 13.087849816230918 5 44 12.013783812730193 5 45 10.077951394844238 5 46 12.247167062771162 5 47 11.462290985143028 5 48 11.084812111714573 5 49 12.765219336076605 5 50 11.661151495882034 5 51 10.80412134700023 5 52 11.021771766141402 5 53 10.81894886716304 5 54 9.72607828975065 5 55 10.220768191545027 5 56 10.956469272368896 6 1 2.4522564913716067 6 2 3.6097789990752673 6 3 3.9729226557884516 6 4 4.284886740729895 6 5 3.6760475984999275 6 6 5.084159631343653 6 7 3.68339325431171 6 8 3.9038071647258406 6 9 4.1916605829538724 6 10 3.4831755335916696 6 11 5.054255770869858 6 12 8.31212440941924 6 13 6.3555303450139675 6 14 7.66893129688631 6 15 7.597781668723677 6 16 7.481608562813715 6 17 7.551131521869234 6 18 6.23682202444347 6 19 4.631548033728986 6 20 6.148509194996603 6 21 3.880837617257022 6 22 3.9779578332034546 6 23 3.7624520514369237 6 24 3.9277806264495183 6 25 3.6230247552560186 6 26 2.438263147155986 6 27 4.1537380800452794 6 28 2.712292154477673 6 29 2.891226055638228 6 30 3.336670812808727 6 31 3.948633572478265 6 32 5.807654288548207 6 33 3.776727235522685 6 34 4.499229930702597 6 35 4.642196258335417 6 36 4.668290726591426 6 37 5.115517419791733 6 38 5.519116410342612 6 39 6.872558401502491 6 40 6.93686490084275 6 41 -2.425719068001463 6 42 7.840086094475154 6 43 11.215179008777504 6 44 9.334103244850866 6 45 7.482565414365087 6 46 10.869616042362999 6 47 9.951199592064057 6 48 10.375406610383603 6 49 12.748804074319729 6 50 9.60746915337982 6 51 10.903943884710966 6 52 11.10251710775631 6 53 11.480439550392848 6 54 11.32102061450664 6 55 9.715444322867121 6 56 9.929406273256209 7 1 1.7702544691269182 7 2 3.490466562270413 7 3 3.765137013725763 7 4 3.64434073466316 7 5 3.589091076760795 7 6 4.720155586854268 7 7 3.3522506860609482 7 8 3.4902581252920655 7 9 3.2629447083330447 7 10 2.445764006796928 7 11 4.424832110606964 7 12 7.427897918013777 7 13 6.22307331771367 7 14 7.92171188333727 7 15 7.003241729391014 7 16 7.969475094664066 7 17 7.912607760494108 7 18 6.227721923331238 7 19 5.3732062743761055 7 20 6.1783373041978145 7 21 4.453638426154903 7 22 4.278261169907196 7 23 3.689145681366149 7 24 3.681066774073379 7 25 3.7170591334157734 7 26 2.530780841797032 7 27 2.9474691215012996 7 28 2.041918039209726 7 29 3.130356490420837 7 30 2.9296940686226804 7 31 3.3217377180798806 7 32 3.8081598497211147 7 33 3.2838050919433144 7 34 3.831889182472068 7 35 4.2999313442808145 7 36 3.0161168135479492 7 37 2.3576812216117524 7 38 3.6652235893112675 7 39 5.399858704839193 7 40 0.9398982678801637 7 41 -7.685577510873051 7 42 6.73139044230124 7 43 10.383530879353842 7 44 9.18749050470931 7 45 6.455265111028382 7 46 9.369616042362999 7 47 8.461816376694998 7 48 8.520502667006458 7 49 11.49754017138747 7 50 9.523040437505198 7 51 9.738625381172039 7 52 9.124761799363998 7 53 9.35051032895706 7 54 9.952466519461137 7 55 9.814028751582995 7 56 9.96833448356966 8 1 2.683401123137358 8 2 3.4899919538223827 8 3 4.216489647802646 8 4 4.4592434399313134 8 5 3.772764546148551 8 6 4.913795833650667 8 7 4.01480407951135 8 8 4.144268566677923 8 9 4.23114594231501 8 10 4.413965240778893 8 11 5.851505105386273 8 12 9.088078269224027 8 13 8.43095181795097 8 14 9.447103435306897 8 15 8.507275901199272 8 16 8.2371542593532 8 17 8.560448292055565 8 18 7.12235884786849 8 19 6.286827536834576 8 20 6.877435548146561 8 21 4.602665478836435 8 22 4.918507966300176 8 23 4.316578049662304 8 24 4.797345843840823 8 25 4.418055811156641 8 26 4.25503333349139 8 27 4.722504717134903 8 28 4.218946990325058 8 29 4.599744245522878 8 30 4.8978953026046455 8 31 4.049787077358474 8 32 6.399047367518925 8 33 4.311332381929077 8 34 3.9828146689457213 8 35 5.2486736318935385 8 36 1.9753004870173392 8 37 1.6581083692149825 8 38 2.7174305185946075 8 39 3.7159479312274257 8 40 -1.1678378498227318 8 41 -9.74870043446109 8 42 6.2620026871992 8 43 9.822543693781938 8 44 8.585212384158762 8 45 6.241216700966685 8 46 9.675738491342592 8 47 9.911270576979767 8 48 9.431750887224776 8 49 11.316239744239866 8 50 7.946865781596323 8 51 10.722488693939003 8 52 11.14422074573324 8 53 11.00262233655079 8 54 10.66485379995473 8 55 10.179951865014417 8 56 9.854428456042367 9 1 2.3364623476271547 9 2 3.4083593007611555 9 3 3.6246529131087755 9 4 4.010263848094581 9 5 3.3237849543118188 9 6 4.26073460916087 9 7 3.484191834613391 9 8 3.1646767299432312 9 9 3.761758187212969 9 10 2.9445774856768523 9 11 4.953545921712802 9 12 7.639098677387295 9 13 6.737074266930563 9 14 8.651185067959954 9 15 7.792990186913563 9 16 7.869807320577689 9 17 7.131876863484141 9 18 7.2039915009297175 9 19 5.348052026630494 9 20 5.530496772636358 9 21 4.133277723734395 9 22 3.73483449691242 9 23 3.643108661907199 9 24 4.042243803024498 9 25 3.2139741785035767 9 26 3.030543537573017 9 27 2.375565941624693 9 28 2.2189469903250583 9 29 3.497703429196349 9 30 3.6121810168903608 9 31 4.253868710011538 9 32 5.766394306294437 9 33 3.3725568717249956 9 34 4.411386097517152 9 35 4.167040978832311 9 36 3.199790282935705 9 37 3.6172920426843724 9 38 2.6766141920639974 9 39 5.511866298574365 9 40 3.260733578748699 9 41 -4.034414720175377 9 42 8.221186360668586 9 43 10.557237571332958 9 44 10.054600139260803 9 45 7.853461598925868 9 46 9.634922164811975 9 47 9.686780781061394 9 48 10.20726109130641 9 49 12.499913213627622 9 50 10.212171904045302 9 51 10.681672367408392 9 52 10.695241153896507 9 53 9.635275397775285 9 54 8.93015992240371 9 55 8.873829416034827 9 56 8.69116314991992 10 1 2.0609521435455207 10 2 3.796114402801976 10 3 4.1178501920203345 10 4 4.108903303876893 10 5 3.7081386958084153 10 6 4.7539318880724295 10 7 3.8583414944773367 10 8 4.11705768232418 10 9 3.6937309763286166 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1.8199239266114944 119 3 2.0622946364647845 119 4 2.6327128276864187 119 5 2.089091076760795 119 6 3.2459953801359234 119 7 1.874214510350356 119 8 1.7757878410543455 119 9 1.7810325636302053 119 10 2.0194074176496386 119 11 -9.535116209806471 119 12 -12.736184769324723 119 13 -2.9681411525705705 119 14 -4.73203488668857 119 15 -8.824923645285985 119 16 -11.78892283815247 119 17 -13.685583453976179 119 18 -1.2438543040589458 119 19 -4.165553415546377 119 20 -2.112360370220788 119 21 0.3373593563874593 119 22 0.6736100071165012 119 23 -1.4646010886596912 119 24 -5.4248763783813985 119 25 -1.6136902205893904 119 26 0.6121761906342442 119 27 0.721370930286831 119 28 -0.9012344155706344 119 29 0.18591204597639432 119 30 -0.10777363163571607 119 31 -1.7325258478115906 119 32 -13.25514764381894 119 33 -2.546944262061853 119 34 -0.4355526779930514 119 35 -1.6742288624375306 119 36 -2.539438742007608 119 37 -1.2999415174063316 119 38 -0.8528642659858896 119 39 1.592365164787516 119 40 4.560053306639855 119 41 2.132251946491291 119 42 -1.5463873354765383 119 43 -4.753420025038922 119 44 -4.9533363686757035 119 45 -3.0705746822532696 119 46 -8.352606179859222 119 47 -13.423196543201641 119 48 -10.370970201210598 119 49 -6.705302205873512 119 50 -6.626830363528395 119 51 -7.004268675675508 119 52 -5.6052123608427245 119 53 -8.712797164582994 119 54 -10.785259578730077 119 55 -8.919821377615968 119 56 -6.295231407903209 120 1 1.227618810212185 120 2 2.3199239266114944 120 3 2.6178501920203345 120 4 2.799379494353083 120 5 2.255757743427459 120 6 3.0634556975962397 120 7 2.382151018286862 120 8 2.545629110895611 120 9 2.312778595376237 120 10 2.2098836081258284 120 11 -8.019243193933455 120 12 -2.394914928054881 120 13 -5.603061787491207 120 14 -8.525685680339365 120 15 -2.023336343698684 120 16 -13.749240298469932 120 17 -11.201456469849195 120 18 -1.8708384310430723 120 19 -1.8560296060225667 120 20 -3.612360370220788 120 21 1.2897403087684083 120 22 1.864086197592691 120 23 1.146510022451416 120 24 -2.7264636799687025 120 25 1.1799605730614005 120 26 3.064557143015193 120 27 2.729307438223337 120 28 2.8924163780801564 120 29 3.177975538039888 120 30 3.408099384237296 120 31 1.2674741521884059 120 32 -7.771020659691956 120 33 2.127658912541321 120 34 -4.935552677993051 120 35 2.357517169308501 120 36 1.25421205164319 120 37 2.9302172127523995 120 38 2.901103987982367 120 39 4.45744452986688 120 40 4.512434259020807 120 41 7.298918613157955 120 42 1.92186663277743 120 43 -0.7216739932928924 120 44 -2.421590336929672 120 45 -7.268987380665969 120 46 -13.154193481446525 120 47 -16.12954574955085 120 48 -9.13287496311536 120 49 -15.744984745556051 120 50 -9.944290680988711 120 51 -9.583633755040587 120 52 -9.406799662430025 120 53 -11.752479704265534 120 54 -15.967799261269759 120 55 -11.03093248872708 120 56 -9.819040931712735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 855.0 5 71.0 6 129.0 7 41.0 8 43.0 9 48.0 10 32.0 11 39.0 12 57.0 13 67.0 14 105.0 15 104.0 16 155.0 17 208.0 18 269.0 19 288.0 20 357.0 21 470.0 22 609.0 23 730.0 24 760.0 25 845.0 26 924.0 27 947.0 28 1090.0 29 1159.0 30 1262.0 31 1521.0 32 1784.0 33 2202.0 34 2497.0 35 3125.0 36 3495.0 37 3575.0 38 2161.0 39 612.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.33509008456919 0.5821791886260571 0.06128201985537443 0.02144870694938105 8 99.0102953793357 0.8671405809535482 0.05821791886260571 0.06434612084814316 9 97.18340076008336 1.8603653303910752 0.3861713865391688 0.570062522986392 10 20.992155901458514 58.515136658904275 8.95943130285574 11.533276136781469 11 16.359235200392206 25.257384483392574 43.258977815908814 15.124402500306411 12 53.02120357886996 12.578134575315602 15.46145360951097 18.939208236303468 13 17.891285696776567 34.4343669567349 15.657556073048168 32.01679127344037 14 41.949126570640516 14.906527735212993 18.397180508734294 24.747165185412197 15 17.200638310931073 36.74277296998711 18.9989566071319 27.05763211194992 16 16.531788363680953 16.050518055300103 45.09226902090614 22.325424560112808 17 45.3272030651341 19.52490421455939 14.36015325670498 20.787739463601532 18 43.75497946926518 25.9851688423117 14.607464607464607 15.65238708095851 19 18.790794594428952 51.34679618790795 12.35252658351975 17.509882634143352 20 18.764940239043824 22.255593012565125 14.5878026356114 44.39166411277965 21 17.813802402549644 21.098921304241237 42.372517773964205 18.71475851924491 22 17.83000367692119 23.409731584753033 31.95244515259223 26.807819585733544 23 18.831964701556565 48.79887241083466 17.410221840911877 14.9589410466969 24 14.664787351391102 49.344282387547494 17.891285696776567 18.09964456428484 25 17.368388796960225 49.89581418152847 17.3714530857388 15.364343935772506 26 27.77590176515733 23.45049884881044 33.72524942440522 15.048349961627014 27 40.03800882785679 20.84968121628249 22.045120156939678 17.06718979892104 28 13.492185340989346 47.60647280989959 25.746921730586177 13.154420118524888 29 12.626417407293902 46.17529880478088 27.93135151700889 13.266932270916335 30 10.743953652331177 21.279465407841094 56.794286239769484 11.182294700058241 31 10.670516325214239 21.688116226925086 57.04456798845102 10.59679945940965 32 36.05663152733513 18.12637901446433 32.96151017406227 12.85547928413827 33 11.749716005035154 44.69006171133831 33.3947376500568 10.16548463356974 34 9.421289907075169 21.476983469807095 35.58745054742847 33.51427607568927 35 35.99411440132426 20.072343817055973 35.733554043283675 8.199987738336093 36 34.555166697366 21.59390925277829 35.26125130472156 8.589672745134157 37 32.989279021902746 20.726200350198138 38.04564863453445 8.238871993364667 38 9.869914708228508 23.875559919003496 58.842118181260346 7.412407191507639 39 9.183516449696857 38.78373803588465 31.769919675006925 20.262825839411565 40 6.2835296294012455 16.140469877289267 25.94499599186039 51.6310045014491 41 24.57262975671582 16.386905313920057 28.071965130297695 30.96849979906643 42 8.771606643947068 24.96085474808879 36.771975069847414 29.49556353811673 43 5.758534861567772 24.466091018037673 27.806287066342993 41.96908705405156 44 6.28677036808871 16.217464962267055 25.630679192977052 51.86508547666718 45 9.144171779141104 46.168711656441715 37.0521472392638 7.634969325153375 46 32.52904926878622 23.334457491492167 36.64040224422847 7.496090995493147 47 8.132050024521824 21.101029916625798 55.29671407552722 15.470205983325158 48 9.713409961685823 23.4911877394636 59.22145593869732 7.573946360153257 49 5.621983831801555 16.469431039252452 25.985921986905602 51.92266314204039 50 8.669348647237662 23.796947455701257 60.55645978564629 6.977244111414796 51 33.080140230026444 23.823728396580353 36.143059228734856 6.953072144658343 52 9.26848775685985 47.54214347237603 35.24978466838932 7.9395841023748 53 30.813167915489498 25.049134013020513 36.25168898169758 7.886009089792409 54 8.4818552716341 22.620325776864323 51.673364213626186 17.224454737875394 55 10.226191572292299 23.97569284596262 35.981953779578305 29.816161802166775 56 34.12125863392172 24.04604758250192 33.80506523407521 8.02762854950115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 3.5 5 7.0 6 11.0 7 15.0 8 16.5 9 18.0 10 26.0 11 34.0 12 68.0 13 156.0 14 210.0 15 237.0 16 264.0 17 285.5 18 307.0 19 337.0 20 367.0 21 426.0 22 588.0 23 691.0 24 867.0 25 1043.0 26 1231.5 27 1420.0 28 1546.5 29 1722.5 30 1772.0 31 1747.5 32 1723.0 33 1538.0 34 1353.0 35 1230.5 36 1108.0 37 934.5 38 733.5 39 706.0 40 703.5 41 701.0 42 699.0 43 697.0 44 866.5 45 1036.0 46 1568.5 47 2494.0 48 2887.0 49 2699.5 50 2512.0 51 2160.0 52 1808.0 53 1694.5 54 1504.0 55 1427.0 56 1203.5 57 980.0 58 860.5 59 741.0 60 665.0 61 589.0 62 530.5 63 441.5 64 411.0 65 342.0 66 273.0 67 211.0 68 149.0 69 128.0 70 107.0 71 80.5 72 39.0 73 24.0 74 18.0 75 12.0 76 9.5 77 7.0 78 5.0 79 3.0 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.024512807942149776 10 0.0 11 0.0 12 0.0 13 0.0 14 0.01838460595661233 15 0.15320504963843606 16 0.0428974138987621 17 0.03370511092045594 18 0.006128201985537444 19 0.009192302978306165 20 0.01838460595661233 21 0.012256403971074888 22 0.0 23 0.0 24 0.0 25 0.006128201985537444 26 0.186910160558892 27 0.03676921191322466 28 0.21142296850104178 29 0.01838460595661233 30 0.03983331290599338 31 0.242063978428729 32 0.012256403971074888 33 0.19916656452996692 34 0.08885892879029293 35 0.0428974138987621 36 0.18997426155166072 37 0.2543203823998039 38 0.12869224169628632 39 0.4381664419659272 40 0.6189484005392818 41 0.8793969849246231 42 0.19916656452996692 43 0.2849613923274911 44 0.5239612697634514 45 0.11030763573967398 46 0.05821791886260571 47 0.03676921191322466 48 0.03370511092045594 49 0.3156024022551783 50 0.22367937247211667 51 0.36156391714670916 52 0.3922049270743964 53 0.22061527147934795 54 0.11337173673244269 55 0.16239735261674224 56 0.186910160558892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 32636.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.31535727417575 #Duplication Level Percentage of deduplicated Percentage of total 1 94.01385030713251 73.6272827552396 2 3.7834031065378144 5.925971320014708 3 0.9663914863648813 2.270498835641623 4 0.3443014202433585 1.07856354945459 5 0.19953832309558278 0.781345753156024 6 0.11737548417387221 0.55153817869837 7 0.08216283892171056 0.4504228459370021 8 0.06651277436519426 0.41671773501654613 9 0.03912516139129074 0.275769089349185 >10 0.32473883954771315 4.9546513053070225 >50 0.007825032278258148 0.41058953303100865 >100 0.05477522594780703 9.25664909915431 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 328 1.0050251256281406 No Hit ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 325 0.9958328226498345 No Hit ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 319 0.9774482166932222 No Hit ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 288 0.8824610859173918 No Hit ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 277 0.848755974996936 No Hit ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 243 0.7445765412427994 No Hit ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 183 0.560730481676676 No Hit ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 178 0.5454099767128325 No Hit ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 172 0.5270253707562201 No Hit ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 170 0.5208971687706827 No Hit ATTCCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 148 0.45348694692977076 No Hit ATTCCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 143 0.4381664419659272 No Hit ATTCCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 128 0.3922049270743964 No Hit ATTCCAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 119 0.36462801813947787 No Hit ATTCCAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 67 0.20529476651550432 No Hit ATTCCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 67 0.20529476651550432 No Hit ATTCCAGGGATGCGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 50 0.15320504963843606 No Hit ATTCCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 42 0.12869224169628632 No Hit ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACACACAGTTCTGAGTCG 42 0.12869224169628632 No Hit ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 39 0.11949993871798015 No Hit ATTCCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 39 0.11949993871798015 No Hit ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTACTCACAGTTCTGAGTCG 38 0.11643583772521142 No Hit ATTCCAGGGATGAGATGGACTTAAATGAATTGACGGGTAACCACAGTTCTGAGTCG 38 0.11643583772521142 No Hit ATTCCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG 36 0.11030763573967398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0030641009927687216 0.0 0.0 0.0 0.0 7 0.0030641009927687216 0.0 0.0 0.0 0.0 8 0.0030641009927687216 0.0 0.0 0.0 0.0 9 0.0030641009927687216 0.0 0.0 0.0 0.0 10 0.015320504963843608 0.0 0.0 0.0 0.0 11 0.024512807942149772 0.0 0.0 0.0 0.0 12 0.027576908934918494 0.0 0.0 0.0 0.0 13 0.03676921191322466 0.0 0.0 0.0 0.0 14 0.03676921191322466 0.0 0.0 0.0 0.0 15 0.04596151489153082 0.0 0.0 0.0 0.0 16 0.04596151489153082 0.0 0.0 0.0 0.0 17 0.04596151489153082 0.0 0.0 0.0 0.0 18 0.04596151489153082 0.0 0.0 0.0 0.0 19 0.052089716877068266 0.0 0.0 0.0 0.0 20 0.05821791886260571 0.0 0.0 0.0 0.0 21 0.05821791886260571 0.0 0.0 0.0 0.0 22 0.06434612084814316 0.0 0.0 0.0 0.0 23 0.06741022184091187 0.0 0.0 0.0 0.0 24 0.07353842382644932 0.0 0.0 0.0 0.0 25 0.08273072680475549 0.0 0.0 0.0 0.0 26 0.0857948277975242 0.0 0.0 0.0 0.0 27 0.08885892879029293 0.0 0.0 0.0 0.0 28 0.09498713077583038 0.0 0.0 0.0 0.0 29 0.09498713077583038 0.0 0.0 0.0 0.0 30 0.09805123176859909 0.0 0.0 0.0 0.0 31 0.10111533276136782 0.0 0.0 0.0 0.0 32 0.10111533276136782 0.0 0.0 0.0 0.0 33 0.10111533276136782 0.0 0.0 0.0 0.0 34 0.10111533276136782 0.0 0.0 0.0 0.0 35 0.10111533276136782 0.0 0.0 0.0 0.0 36 0.10111533276136782 0.0 0.0 0.0 0.0 37 0.10111533276136782 0.0 0.0 0.0 0.0 38 0.10111533276136782 0.0 0.0 0.0 0.0 39 0.10111533276136782 0.0 0.0 0.0 0.0 40 0.10111533276136782 0.0 0.0 0.0 0.0 41 0.10111533276136782 0.0 0.0 0.0 0.0 42 0.10724353474690526 0.0 0.0 0.0 0.0 43 0.11030763573967398 0.0 0.0 0.0 0.0 44 0.11030763573967398 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAACC 30 9.4206916E-7 50.551403 38 GCCCAGC 45 1.1459633E-10 50.551403 41 CAACCTG 15 0.0080024665 50.551403 41 CAACCAA 15 0.0080024665 50.551403 40 ACCAACC 15 0.0080024665 50.551403 38 CAACAAA 25 1.913756E-5 50.551403 40 TACGCCC 230 0.0 50.551403 38 TACGTCC 15 0.0080024665 50.551403 38 CCTTAAA 15 0.0080024665 50.551403 40 TCCGCCC 95 0.0 50.551403 38 GACCAGC 15 0.0080024665 50.551403 41 CGACCAG 25 1.913756E-5 50.551403 40 CGTCCAG 15 0.0080024665 50.551403 40 CCAACAA 20 3.9057387E-4 50.5514 39 ACGCCAA 20 3.9057387E-4 50.5514 39 AGCCATA 20 3.9057387E-4 50.5514 38 CGCCAAG 20 3.9057387E-4 50.5514 40 TACGACC 20 3.9057387E-4 50.5514 38 CATAGAA 20 3.9057387E-4 50.5514 41 CCATAGA 20 3.9057387E-4 50.5514 40 >>END_MODULE