FastQCFastQC Report
Fri 17 Jun 2016
SRR1526286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC470.2606188310968171No Hit
CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA300.1663524453809471No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC290.16080736386824887No Hit
CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA280.15526228235555065No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG280.15526228235555065No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC260.14417211933015417No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC250.13862703781745592No Hit
CTGAAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC190.10535654874126649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCA150.00809121150.31741736
TGTGAGG150.00818180350.1764747
CACCGAC150.00818180350.1764730
GACGGAT150.00818180350.1764732
GGTTCGG150.00818180350.1764731
TAAGACC150.00818180350.1764735
TCCAGCC150.00818180350.1764735
GTGAGGG150.00818180350.1764748
GCTACCC150.00818180350.1764733
CCACCGA150.00818180350.1764729
TCTGTGA150.00818180350.1764745
CAGGGTC150.00818180350.1764730
CTGTGAG150.00818180350.1764746
GACGATC150.00818180350.1764734
TGAGGGT150.00818180350.1764749
CGACGAT150.00818180350.1764733
AGCGCCG150.00818180350.1764745
GAGGGTG150.00818180350.1764750
CGGTTCG150.00818180350.1764730
TTCGGCG150.00818180350.1764733