##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526286_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18034 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.70938227791949 39.0 38.0 40.0 34.0 40.0 2 37.171786625263394 39.0 38.0 40.0 33.0 40.0 3 36.742042808029275 39.0 36.0 40.0 31.0 40.0 4 35.54247532438727 39.0 36.0 40.0 23.0 40.0 5 35.52811356326938 39.0 36.0 40.0 22.0 40.0 6 34.0852833536653 38.0 35.0 39.0 14.0 40.0 7 36.19524232006211 38.0 35.0 39.0 29.0 40.0 8 36.22712653876012 38.0 35.0 39.0 30.0 40.0 9 36.34756570921593 38.0 36.0 39.0 29.0 40.0 10 35.576854829766 38.0 35.0 39.0 27.0 40.0 11 34.43700787401575 38.0 33.0 39.0 25.0 40.0 12 31.992181435067096 36.0 28.0 39.0 16.0 40.0 13 31.65437506931352 35.0 28.0 39.0 15.0 40.0 14 31.28119108350893 36.0 26.0 39.0 13.0 40.0 15 32.01674614616835 36.0 28.0 39.0 16.0 40.0 16 32.70511256515471 36.0 30.0 39.0 18.0 40.0 17 32.57297327270711 36.0 30.0 39.0 18.0 40.0 18 32.76694022402129 36.0 30.0 39.0 19.0 40.0 19 33.72707108794499 36.0 31.0 39.0 24.0 40.0 20 33.832316735056004 37.0 31.0 39.0 24.0 40.0 21 35.38371964067872 38.0 35.0 39.0 27.0 40.0 22 34.936120660973714 38.0 34.0 39.0 26.0 40.0 23 35.21775535100366 38.0 35.0 39.0 27.0 40.0 24 34.7768104691139 38.0 34.0 39.0 25.0 40.0 25 35.09382277919485 38.0 35.0 39.0 27.0 40.0 26 35.26577575690363 38.0 35.0 39.0 27.0 40.0 27 35.26566485527337 38.0 35.0 39.0 27.0 40.0 28 35.12071642453144 38.0 35.0 39.0 27.0 40.0 29 34.92536320283908 38.0 35.0 39.0 27.0 40.0 30 34.74037928357547 38.0 35.0 39.0 26.0 40.0 31 34.33935898857713 38.0 33.0 39.0 24.0 40.0 32 33.36664078961961 38.0 33.0 39.0 18.0 40.0 33 34.52933348120217 38.0 34.0 39.0 25.0 40.0 34 34.264167683264944 38.0 33.0 39.0 24.0 40.0 35 34.30897194188755 38.0 34.0 39.0 25.0 40.0 36 32.51142286791616 35.0 31.0 38.0 23.0 39.0 37 32.28424087834091 35.0 31.0 38.0 21.0 39.0 38 31.58472884551403 35.0 30.0 38.0 16.0 39.0 39 29.603249417766442 34.0 27.0 38.0 6.0 39.0 40 25.883276034157703 31.0 13.0 37.0 4.0 39.0 41 22.576411223244982 26.0 10.0 33.0 4.0 37.0 42 25.975102584007985 30.0 21.0 34.0 4.0 37.0 43 22.13507818564933 26.0 9.0 33.0 4.0 37.0 44 23.17661084617944 28.0 10.0 34.0 4.0 37.0 45 25.60840634357325 30.0 21.0 34.0 4.0 38.0 46 25.41050238438505 30.0 17.0 36.0 4.0 38.0 47 24.74015748031496 30.0 11.0 36.0 4.0 38.0 48 24.636852611733392 30.0 11.0 35.0 4.0 38.0 49 22.262670511256516 27.0 5.0 35.0 4.0 38.0 50 24.525063768437395 30.0 10.0 35.0 4.0 38.0 51 25.942053898192302 32.0 4.0 38.0 4.0 40.0 52 25.690418099146058 32.0 4.0 38.0 4.0 40.0 53 25.320339358988576 31.0 4.0 38.0 4.0 39.0 54 24.350837307308417 31.0 4.0 37.0 4.0 39.0 55 24.636020849506487 31.0 4.0 37.0 4.0 39.0 56 24.18686924697793 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7436829641453713 1 2 1.186509444911927 1 3 1.891717686700396 1 4 3.6816588358563607 1 5 3.387928825659934 1 6 5.1467367534821165 1 7 2.7444734842434357 1 8 2.634482077998193 1 9 2.6559530086523466 1 10 2.297966120809349 1 11 -3.8671236819570183 1 12 -8.37435221837898 1 13 -21.87156259138174 1 14 -17.820197755012064 1 15 -10.386649643358371 1 16 -21.919115817096465 1 17 -21.118375700182252 1 18 -19.939399550304458 1 19 -7.507805579013976 1 20 -11.481008456199046 1 21 -7.230259852820666 1 22 -2.908379752974465 1 23 -4.354776292889898 1 24 -3.6864439175872086 1 25 -3.6150351032565453 1 26 1.0276599672246292 1 27 1.497998460314605 1 28 0.6326225773152885 1 29 -0.540061865054156 1 30 0.716347607267906 1 31 0.42724896455653294 1 32 -12.947056550046348 1 33 -0.2045904050361642 1 34 -0.11207697561639662 1 35 -0.8316349533644782 1 36 2.193229411542376 1 37 2.251754514050834 1 38 2.5257239770484183 1 39 4.713126663254197 1 40 7.687060415547155 1 41 -0.4894236288584537 1 42 -7.871945851232802 1 43 -13.7227969833876 1 44 -15.888660878981357 1 45 -14.660652870087443 1 46 -18.964034940090308 1 47 -18.056209286563455 1 48 -17.47995711914205 1 49 -15.206041103906525 1 50 -17.577691997099173 1 51 -18.357867372761724 1 52 -18.1547070417544 1 53 -17.687816120002246 1 54 -18.0440430431488 1 55 -18.114552969113298 1 56 -17.852372297865166 2 1 0.9217367943731176 2 2 1.3459297347670045 2 3 1.5211172726217228 2 4 4.133004591549941 2 5 3.69020625837215 2 6 5.291664289713999 2 7 2.4877447057755333 2 8 2.7649168606068883 2 9 2.4344209175550375 2 10 2.6747777150122474 2 11 -1.7884487337168515 2 12 -13.101060292913143 2 13 -13.215247891588778 2 14 -13.18044620221703 2 15 -4.8794032665467775 2 16 -3.153070268442221 2 17 -1.9838001308240756 2 18 -9.707515492333442 2 19 -2.543002266384576 2 20 -3.719103694294283 2 21 1.717980312810802 2 22 1.6526968515804015 2 23 0.7528841625966436 2 24 2.1520653991829803 2 25 1.3953168636171611 2 26 0.9862520997298034 2 27 2.9017251683891416 2 28 2.1667840679985204 2 29 2.3791927933309367 2 30 2.5320825969159344 2 31 -1.4878649070790786 2 32 -12.456373320232682 2 33 -2.9644247735661864 2 34 -1.45990306257292 2 35 -2.868902034109823 2 36 2.681842247981301 2 37 3.4877793587713306 2 38 4.73276331452254 2 39 4.402567657043019 2 40 8.074223976623752 2 41 6.59132171275645 2 42 1.0825054945228914 2 43 1.6995632650596058 2 44 -8.491145351031047 2 45 0.9790986827075905 2 46 -15.402958335535441 2 47 -16.124532267929915 2 48 -13.115567885187598 2 49 -14.311631166018326 2 50 -14.066304833538094 2 51 -13.974844598434602 2 52 -13.701290892686075 2 53 -14.62570431876001 2 54 -16.52230391271402 2 55 -14.071074708243733 2 56 -13.200198384821688 3 1 2.050561271023682 3 2 2.667990926393408 3 3 3.0927758877585987 3 4 4.612875767073291 3 5 4.9381933759244845 3 6 6.7022923090376665 3 7 3.532833272603227 3 8 3.560408003924117 3 9 3.756482109181441 3 10 4.483151305994539 3 11 3.8524530376197035 3 12 3.916653072626307 3 13 -1.3054250252441726 3 14 -7.333425268239576 3 15 0.13186887516014778 3 16 3.5570746590940097 3 17 3.4477618659553144 3 18 3.171711560806653 3 19 2.8096547384463406 3 20 2.0216370464464575 3 21 4.007835385274575 3 22 4.673630829036114 3 23 3.6240596859460794 3 24 4.567524336381048 3 25 4.485493997272556 3 26 3.9959139354785975 3 27 4.4186333809495295 3 28 4.034738979431694 3 29 4.438774113781818 3 30 4.652855543775843 3 31 4.60714314445071 3 32 3.8624672594774587 3 33 3.4091662087204497 3 34 5.073108209568787 3 35 4.369423247693717 3 36 6.812277030589996 3 37 6.854929117225435 3 38 8.091861543185981 3 39 9.549105499233033 3 40 12.417219145705882 3 41 12.902110762675935 3 42 9.974614995328047 3 43 5.504716244125628 3 44 6.756841766521287 3 45 10.937230727796155 3 46 6.358716382661015 3 47 -0.415997646351812 3 48 7.006815367630438 3 49 -5.475882373747796 3 50 6.6180751986680235 3 51 3.6209686060742534 3 52 3.8558749688276137 3 53 2.619062186876061 3 54 1.7337347346289782 3 55 2.366928512368183 3 56 6.592072146579277 4 1 1.7198734403358529 4 2 2.0555570639595473 4 3 2.951241496224206 4 4 4.44356359776112 4 5 4.828405016136124 4 6 6.420546277291635 4 7 2.4706639604339173 4 8 3.0987677922839083 4 9 3.2869053896047262 4 10 4.0955851684284 4 11 5.609066794233456 4 12 2.839933495906731 4 13 7.461770741951593 4 14 6.108373673559363 4 15 7.26811226140353 4 16 7.354693706713061 4 17 7.322100490293941 4 18 6.620124259219356 4 19 5.587432516224119 4 20 5.530896305705717 4 21 3.7816449090840933 4 22 4.008286913692203 4 23 3.8118903737767695 4 24 3.563556082412788 4 25 2.5754410872196445 4 26 2.2300409196055853 4 27 2.890855603171751 4 28 2.9897654344581497 4 29 3.4956524206601287 4 30 3.0972999882202856 4 31 4.2605822978898615 4 32 5.5529434499536485 4 33 0.9144572140114491 4 34 2.816494452955034 4 35 3.239793618064091 4 36 5.41941988773285 4 37 6.4303259426222645 4 38 6.656676358000798 4 39 7.248840948968482 4 40 10.996584225070958 4 41 8.403433513998689 4 42 8.675673196386242 4 43 8.455774445183831 4 44 11.718481978161499 4 45 8.089347129912557 4 46 7.3454888694335025 4 47 6.586647856293688 4 48 6.674804785619855 4 49 1.5796731818077596 4 50 6.910403240996068 4 51 5.796894532000181 4 52 5.059578672531316 4 53 4.7407553085691845 4 54 0.5630998139940573 4 55 5.885447030886702 4 56 4.004770559277691 5 1 1.9394501599125675 5 2 2.260583518986003 5 3 3.1298129247956368 5 4 4.649912804110329 5 5 4.715971153702263 5 6 6.961551568296926 5 7 3.5698703096402653 5 8 3.4863339298500478 5 9 3.5342598869592194 5 10 3.6683364911797227 5 11 5.556156741323406 5 12 7.509245665218902 5 13 8.101982382163232 5 14 6.555463620649313 5 15 8.094831838123106 5 16 6.038556140575491 5 17 5.892206310399757 5 18 3.72726711636221 5 19 3.587432516224119 5 20 4.4660814908909 5 21 4.044872422311613 5 22 3.5254826808879685 5 23 4.1796152415016365 5 24 5.049005817862529 5 25 3.6706791824577394 5 26 2.9588768984415594 5 27 3.8630778253939724 5 28 3.2569612016539153 5 29 3.4758111508188563 5 30 3.6158185067388047 5 31 3.4589949963025646 5 32 4.825430222440421 5 33 1.7424995420537783 5 34 2.739774876235458 5 35 2.665719543990015 5 36 3.0715362898492558 5 37 3.743818006114328 5 38 4.462231913556355 5 39 5.549105499233033 5 40 4.3061080345947715 5 41 3.2724811330463055 5 42 9.011652032365078 5 43 11.801012540421926 5 44 10.75684176652129 5 45 8.86315665372208 5 46 9.8401978641425 5 47 11.695113464759299 5 48 10.525333886148957 5 49 10.894487996622573 5 50 10.544001124593951 5 51 8.732079717185364 5 52 9.67068978364243 5 53 9.582025149839026 5 54 8.104105104999348 5 55 9.403965549405221 5 56 8.555035109542242 6 1 1.584405460040287 6 2 2.106049674796985 6 3 2.509123269623224 6 4 4.079031578056686 6 5 4.726188267367647 6 6 6.261679282217749 6 7 2.9300235663452483 6 8 3.01379242282578 6 9 3.022127064481573 6 10 3.389920143797859 6 11 5.210052015908332 6 12 7.078849752064368 6 13 7.900194387271792 6 14 8.27832441247562 6 15 7.808752655492199 6 16 6.68351144070321 6 17 6.825795071574724 6 18 5.278991254293242 6 19 5.587432516224119 6 20 4.522275616050543 6 21 3.3296744657343424 6 22 3.733656371820281 6 23 3.6419396348605133 6 24 3.835723570097521 6 25 2.9350469985496943 6 26 3.37905569793071 6 27 2.020165947999338 6 28 1.0513417891379504 6 29 3.37619429258131 6 30 3.304196539944421 6 31 3.420680820057349 6 32 4.644076454879762 6 33 3.6262798741099758 6 34 4.025854058866365 6 35 2.532897066339956 6 36 1.708828754727925 6 37 2.586729883508962 6 38 3.906676358000798 6 39 4.5554911952739054 6 40 -1.6019379424167255 6 41 3.1115615928164218 6 42 8.01803772840595 6 43 11.513656218582845 6 44 9.406905623481698 6 45 6.8861451594692085 6 46 11.126277046773406 6 47 11.65807642772226 6 48 11.227760450644482 6 49 12.217604216290521 6 50 10.69981210799114 6 51 8.51241177337949 6 52 7.8699235001175225 6 53 8.09543511152485 6 54 10.611129370644303 6 55 7.954412548128083 6 56 9.113144943514143 7 1 1.9451481656105756 7 2 2.5198427782452626 7 3 2.5144283094110236 7 4 3.8336734878710104 7 5 4.4410423787734885 7 6 6.1980188047641676 7 7 3.1767079164778735 7 8 3.4806359241520397 7 9 1.8775647302640621 7 10 3.862068684911918 7 11 5.422253607420274 7 12 7.960812616785855 7 13 8.445287225468075 7 14 8.190791255976947 7 15 6.677452920744194 7 16 7.159638761658112 7 17 6.929243347436795 7 18 6.76572865482375 7 19 4.472047900839506 7 20 5.780896305705717 7 21 3.097578975018159 7 22 4.313231968637254 7 23 2.7679343298207257 7 24 3.494874763731474 7 25 4.157858669637228 7 26 2.9745464141110745 7 27 3.4870094493255976 7 28 2.8908643355570476 7 29 3.4159820909898 7 30 3.5972999882202856 7 31 4.326516363823927 7 32 4.003492900503101 7 33 3.2111605107147483 7 34 3.69561533207591 7 35 1.8167166949871714 7 36 1.158430876743843 7 37 0.08142484372116598 7 38 2.2528302041546446 7 39 2.564775014902551 7 40 0.10097982946656359 7 41 -7.791621431056257 7 42 5.335013855726903 7 43 9.634345873755258 7 44 8.413536923216444 7 45 5.542643833209262 7 46 9.642192166136798 7 47 10.965768735414567 7 48 9.402826763641833 7 49 12.771980874115453 7 50 9.110952691545517 7 51 11.700740685846334 7 52 10.021117134069776 7 53 9.295700363514236 7 54 11.87903387992812 7 55 9.731600877040549 7 56 8.839935394442527 8 1 2.1663020117644223 8 2 2.3948427782452626 8 3 3.046479591462301 8 4 4.913801692999215 8 5 5.114119301850408 8 6 6.7717367534821165 8 7 3.708759198529151 8 8 3.4678154113315287 8 9 3.90000062769996 8 10 4.422966120809349 8 11 5.579304889471551 8 12 8.393504924478162 8 13 8.092723122903973 8 14 8.87027843546413 8 15 8.44668368997496 8 16 7.515407992427345 8 17 6.262576680770131 8 18 6.810600449695546 8 19 5.920765849557455 8 20 5.364229639039053 8 21 3.9245020519412392 8 22 4.5440011994064875 8 23 3.633318945205339 8 24 4.248079891936598 8 25 4.254012515791075 8 26 1.8848028243674904 8 27 3.140855603171751 8 28 1.3171463868390987 8 29 3.6563667063744134 8 30 1.9306333215536213 8 31 1.8201061074136717 8 32 4.612467259477459 8 33 3.3489810235352593 8 34 3.1795896910502677 8 35 3.114793618064091 8 36 1.770610363923332 8 37 0.34104022833654923 8 38 1.74000969133413 8 39 3.6833647584922957 8 40 -2.6105586320718963 8 41 -9.477518866953693 8 42 5.395911291624341 8 43 9.71767920708859 8 44 7.2661010257805465 8 45 6.321489987055415 8 46 9.82167934562398 8 47 10.28307642772226 8 48 10.168852404667476 8 49 11.996339848474427 8 50 9.886593717186546 8 51 11.79689453200018 8 52 11.72624533919798 8 53 10.359802927616805 8 54 13.164290290184535 8 55 12.052113697553366 8 56 10.314294368801498 9 1 1.749635345097758 9 2 2.3531761115785983 9 3 1.796479591462301 9 4 4.2471350263325505 9 5 4.655785968517073 9 6 6.438403420148781 9 7 3.833759198529151 9 8 2.4261487446648573 9 9 3.3166672943666313 9 10 4.756299454142685 9 11 4.6209715561382225 9 12 7.935171591144826 9 13 8.301056456237308 9 14 7.328611768797458 9 15 7.571683689974961 9 16 6.30707465909401 9 17 6.09591001410346 9 18 5.393933783028874 9 19 5.7540991828907835 9 20 4.405896305705717 9 21 2.882835385274575 9 22 3.502334532739823 9 23 3.7999856118720032 9 24 5.0397465586032695 9 25 1.5456791824577394 9 26 1.6764694910341547 9 27 3.0991889365050866 9 28 1.9004797201724344 9 29 2.864700039707749 9 30 2.3056333215536213 9 31 3.111772774080343 9 32 4.820800592810794 9 33 3.3906476902019307 9 34 3.1795896910502677 9 35 2.9064602847307555 9 36 2.2289436972566605 9 37 2.424373561669885 9 38 3.2400096913341336 9 39 5.975031425158964 9 40 0.5561080345947715 9 41 -0.019185533620358797 9 42 7.687577958291008 9 43 13.092679207088594 9 44 9.891101025780547 9 45 7.488156653722083 9 46 10.40501267895731 9 47 11.574743094388925 9 48 10.71051907133414 9 49 12.371339848474424 9 50 10.17826038385321 9 51 11.380227865333516 9 52 10.809578672531316 9 53 11.109802927616805 9 54 11.997623623517864 9 55 12.260447030886702 9 56 13.48096103546817 10 1 2.3297635502259624 10 2 2.596765855168343 10 3 3.5528898478725566 10 4 4.256750410947937 10 5 5.248734686465795 10 6 7.044172650918014 10 7 3.5228617626317202 10 8 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116 50 -6.982453901861076 116 51 -5.810248325142677 116 52 -6.440421327468684 116 53 -7.544958977145102 116 54 -7.758328757434514 116 55 -5.543124397684725 116 56 -3.709515155008024 117 1 0.4913020117644251 117 2 1.2198427782452654 117 3 1.5298129247956354 117 4 3.272135026332549 117 5 3.564119301850411 117 6 3.0134034201487836 117 7 1.4920925318624896 117 8 1.0344820779981916 117 9 1.4083339610332928 117 10 1.9312994541426818 117 11 -12.462361777195113 117 12 -10.631495075521837 117 13 -12.423943543762693 117 14 -7.763054897869207 117 15 -5.453316310025038 117 16 -10.309592007572654 117 17 -6.570756652563205 117 18 -5.072732883637791 117 19 -2.7125674837758815 117 20 -3.719103694294283 117 21 -1.125497948058758 117 22 -1.4559988005935125 117 23 -0.7166810547946625 117 24 -3.343586774730067 117 25 0.004012515791075089 117 26 0.45146949103415324 117 27 2.0408556031717495 117 28 1.3754797201724358 117 29 2.7313667063744163 117 30 0.29729998822028847 117 31 -2.196560559252994 117 32 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18 -2.27273288363779 120 19 -2.412567483775881 120 20 -2.719103694294283 120 21 -1.0178056403664542 120 22 0.1209242763295677 120 23 -1.7320656701792743 120 24 -11.851279082422373 120 25 -2.9190644072858483 120 26 1.0130079525726146 120 27 0.7947017570179042 120 28 2.3908643355570476 120 29 2.0313667063744134 120 30 0.17422306514336583 120 31 -5.404252866945299 120 32 -12.419584022573822 120 33 -5.981147181592945 120 34 -13.419769283308703 120 35 -5.798667920397449 120 36 -3.572338354025387 120 37 -2.8801136178172975 120 38 -3.1702467189222787 120 39 0.21862116874870452 120 40 -6.591327862841126 120 41 -4.830082969517797 120 42 -7.164986144273097 120 43 -7.788731049321665 120 44 -11.240309230629709 120 45 -11.341971551406123 120 46 -14.473192449247817 120 47 -15.34192357227774 120 48 -13.751019390204322 120 49 -15.381865279730702 120 50 -12.504431923839098 120 51 -12.22233623723059 120 52 -12.132729019776376 120 53 -12.7427611749473 120 54 -14.81327381237957 120 55 -12.114552969113298 120 56 -8.698526144019013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 180.0 5 62.0 6 13.0 7 17.0 8 28.0 9 15.0 10 12.0 11 29.0 12 17.0 13 38.0 14 59.0 15 74.0 16 108.0 17 138.0 18 163.0 19 199.0 20 260.0 21 356.0 22 376.0 23 475.0 24 504.0 25 541.0 26 534.0 27 591.0 28 652.0 29 661.0 30 689.0 31 727.0 32 958.0 33 1157.0 34 1441.0 35 1614.0 36 1871.0 37 1926.0 38 1121.0 39 428.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.6783852722635 0.2717089941222136 0.03881557058888766 0.011090163025396473 8 99.7282910058778 0.15526228235555065 0.08872130420317179 0.027725407563491185 9 99.52856350526899 0.2273987798114254 0.13865779256794233 0.10537992235163617 10 77.0378174559166 14.688920927137627 2.3843850504602417 5.888876566485527 11 43.56770544527004 27.215260064322944 13.707441499390042 15.509592991016968 12 34.11888654763225 14.428302096040813 21.869801486081844 29.583009870245093 13 24.991682377730953 15.043806143950317 25.673727403792835 34.290784074525895 14 20.401486164254422 16.231353629457107 28.99129373925581 34.375866467032665 15 21.190066114784152 19.251069503861327 29.684982499027722 29.873881882326796 16 26.86012317594185 21.317205792598344 24.074793319647117 27.747877711812684 17 28.377253814147018 24.52149791955617 22.246879334257976 24.854368932038835 18 28.309210891144016 26.15759995563689 21.249930682637384 24.28325847058171 19 28.573013144029723 26.016305252065884 20.498031168543065 24.91265043536132 20 27.706299911268857 29.076086956521742 20.97382431233363 22.243788819875775 21 28.471606033717833 27.933673469387756 21.51175687666371 22.0829636202307 22 26.64411666851503 26.777198624819786 23.666407896196073 22.912276810469116 23 28.751247643340356 26.843739602972168 22.540756349118332 21.864256404569147 24 26.538760119773762 27.92503049794832 20.893867139846954 24.642342242430963 25 25.862260175224577 29.616280359321284 20.666518797826328 23.854940667627815 26 23.607869289763254 28.80404579304213 22.379682116260977 25.208402800933644 27 25.29532471854029 25.417336808829237 23.076923076923077 26.210415395707393 28 22.97822706065319 29.46011997333926 23.53921350810931 24.022439457898244 29 25.715394853593608 28.88753327417924 21.48402839396628 23.91304347826087 30 23.790769913467937 27.607055691147103 25.465941868205018 23.136232527179942 31 25.348746734841328 26.04901906296893 25.19313066192408 23.409103540265658 32 28.118240807498196 24.369142033165105 22.489046641894515 25.023570517442185 33 29.154162498610646 26.48660664666 22.974324774924977 21.38490607980438 34 24.938943161634104 26.931616341030196 26.321047957371224 21.808392539964476 35 23.49350793474642 30.88447453112862 27.05027188991233 18.57174564421263 36 24.9097272373757 30.464974168101772 23.915338036775736 20.709960557746793 37 24.931917968098706 29.14466737064414 24.181626187961985 21.74178847329517 38 24.525369157322082 32.06950149883424 24.786277339846784 18.618852003996892 39 23.530395052097845 23.803421184599095 22.5330138741851 30.133169889117962 40 15.702710254260968 17.21709974853311 18.066499022073206 49.01369097513272 41 17.03985669502911 19.536497984773845 20.818405732198837 42.605239587998206 42 22.441382375819536 28.975441715746193 25.769529947771975 22.813645960662296 43 14.897288871569337 15.776874686856315 20.558926682625398 48.76690975894895 44 15.13815238626849 18.146804353893387 19.888361708065865 46.82668155177226 45 23.405672420491758 27.3075428761725 27.179885663539988 22.10689903979575 46 23.566949669829643 28.222629154874866 24.560235281060987 23.650185894234504 47 18.83736410028844 25.782116707344134 24.92234302196583 30.458176170401597 48 23.088015085131165 29.682213964838333 26.504353613221674 20.725417336808828 49 15.655272241398507 17.937868834205545 19.99220576773188 46.41465315666407 50 20.507834203800424 27.553061451272363 28.575397266362927 23.363707078564286 51 22.323614511462274 30.319385711106168 26.886267527264636 20.470732250166925 52 22.123893805309734 30.83987310068459 25.31863972839094 21.717593365614736 53 23.52941176470588 27.438007339041476 28.4387857222284 20.59379517402424 54 20.518514406262145 24.9986121134736 24.343529673013933 30.13934380725032 55 23.069658926785912 27.50249972225308 26.90812131985335 22.519720031107653 56 22.817934329684984 28.273792988499363 25.60142230123896 23.306850380576698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 3.5 9 4.0 10 6.0 11 8.0 12 41.0 13 115.5 14 157.0 15 198.5 16 240.0 17 253.5 18 267.0 19 270.5 20 274.0 21 266.5 22 254.0 23 249.0 24 265.0 25 281.0 26 279.0 27 277.0 28 298.5 29 309.0 30 298.0 31 294.0 32 290.0 33 293.0 34 296.0 35 279.5 36 263.0 37 241.0 38 215.5 39 212.0 40 207.0 41 202.0 42 241.0 43 280.0 44 311.5 45 343.0 46 360.0 47 401.5 48 426.0 49 446.5 50 467.0 51 561.5 52 656.0 53 708.0 54 861.0 55 962.0 56 1080.5 57 1199.0 58 1313.0 59 1427.0 60 1413.5 61 1400.0 62 1329.0 63 1202.0 64 1146.0 65 1049.0 66 952.0 67 835.0 68 718.0 69 634.0 70 550.0 71 450.5 72 319.5 73 288.0 74 212.5 75 137.0 76 108.0 77 79.0 78 60.5 79 27.5 80 13.0 81 9.0 82 5.0 83 4.0 84 3.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.022180326050792946 10 0.0 11 0.0 12 0.0 13 0.0 14 0.005545081512698237 15 0.19407785294443827 16 0.06099589663968061 17 0.04990573361428413 18 0.005545081512698237 19 0.01663524453809471 20 0.011090163025396473 21 0.011090163025396473 22 0.0 23 0.0 24 0.0 25 0.0 26 0.22180326050792948 27 0.01663524453809471 28 0.1663524453809471 29 0.011090163025396473 30 0.03327048907618942 31 0.2273483420206277 32 0.01663524453809471 33 0.22180326050792948 34 0.09981146722856826 35 0.06654097815237885 36 0.18298768991904182 37 0.2273483420206277 38 0.11090163025396474 39 0.4824220916047466 40 0.7707663302650549 41 0.9426638571587003 42 0.19962293445713652 43 0.39370078740157477 44 0.6598647000110902 45 0.09426638571587002 46 0.07208605966507708 47 0.03327048907618942 48 0.01663524453809471 49 0.39924586891427305 50 0.19962293445713652 51 0.3437950537872907 52 0.37152046135078187 53 0.2661639126095154 54 0.11644671176666296 55 0.17744260840634357 56 0.19407785294443827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 18034.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.0337140955972 #Duplication Level Percentage of deduplicated Percentage of total 1 93.09019904258 81.95075967616724 2 4.648526077097506 8.184540312742596 3 1.1085915847820609 2.927803038704669 4 0.4661123708742756 1.641344127758678 5 0.22675736961451248 0.9981146722856826 6 0.10078105316200556 0.5323278252190308 7 0.0818846056941295 0.5046024176555395 8 0.07558578987150416 0.5323278252190308 9 0.05668934240362812 0.4491516025285571 >10 0.14487276392038298 2.2790285017189755 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 47 0.2606188310968171 No Hit CTGAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 30 0.1663524453809471 No Hit CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 29 0.16080736386824887 No Hit CTGAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 28 0.15526228235555065 No Hit CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 28 0.15526228235555065 No Hit CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 26 0.14417211933015417 No Hit CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 25 0.13862703781745592 No Hit CTGAAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC 19 0.10535654874126649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.005545081512698237 0.0 0.0 0.0 0.0 11 0.011090163025396473 0.0 0.0 0.0 0.0 12 0.011090163025396473 0.0 0.0 0.0 0.0 13 0.011090163025396473 0.0 0.0 0.0 0.0 14 0.011090163025396473 0.0 0.0 0.0 0.0 15 0.011090163025396473 0.0 0.0 0.0 0.0 16 0.011090163025396473 0.0 0.0 0.0 0.0 17 0.01663524453809471 0.0 0.0 0.0 0.0 18 0.01663524453809471 0.0 0.0 0.0 0.0 19 0.01663524453809471 0.0 0.0 0.0 0.0 20 0.01663524453809471 0.0 0.0 0.0 0.0 21 0.01663524453809471 0.0 0.0 0.0 0.0 22 0.01663524453809471 0.0 0.0 0.0 0.0 23 0.01663524453809471 0.0 0.0 0.0 0.0 24 0.01663524453809471 0.0 0.0 0.0 0.0 25 0.01663524453809471 0.0 0.0 0.0 0.0 26 0.022180326050792946 0.0 0.0 0.0 0.0 27 0.022180326050792946 0.0 0.0 0.0 0.0 28 0.027725407563491185 0.0 0.0 0.0 0.0 29 0.027725407563491185 0.0 0.0 0.0 0.0 30 0.027725407563491185 0.0 0.0 0.0 0.0 31 0.027725407563491185 0.0 0.0 0.0 0.0 32 0.027725407563491185 0.0 0.0 0.0 0.0 33 0.027725407563491185 0.0 0.0 0.0 0.0 34 0.027725407563491185 0.0 0.0 0.0 0.0 35 0.027725407563491185 0.0 0.0 0.0 0.0 36 0.027725407563491185 0.0 0.0 0.0 0.0 37 0.027725407563491185 0.0 0.0 0.0 0.0 38 0.027725407563491185 0.0 0.0 0.0 0.0 39 0.027725407563491185 0.0 0.0 0.0 0.0 40 0.027725407563491185 0.0 0.0 0.0 0.0 41 0.027725407563491185 0.0 0.0 0.0 0.0 42 0.027725407563491185 0.0 0.0 0.0 0.0 43 0.027725407563491185 0.0 0.0 0.0 0.0 44 0.03327048907618942 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACCA 15 0.008091211 50.317417 36 TGTGAGG 15 0.008181803 50.17647 47 CACCGAC 15 0.008181803 50.17647 30 GACGGAT 15 0.008181803 50.17647 32 GGTTCGG 15 0.008181803 50.17647 31 TAAGACC 15 0.008181803 50.17647 35 TCCAGCC 15 0.008181803 50.17647 35 GTGAGGG 15 0.008181803 50.17647 48 GCTACCC 15 0.008181803 50.17647 33 CCACCGA 15 0.008181803 50.17647 29 TCTGTGA 15 0.008181803 50.17647 45 CAGGGTC 15 0.008181803 50.17647 30 CTGTGAG 15 0.008181803 50.17647 46 GACGATC 15 0.008181803 50.17647 34 TGAGGGT 15 0.008181803 50.17647 49 CGACGAT 15 0.008181803 50.17647 33 AGCGCCG 15 0.008181803 50.17647 45 GAGGGTG 15 0.008181803 50.17647 50 CGGTTCG 15 0.008181803 50.17647 30 TTCGGCG 15 0.008181803 50.17647 33 >>END_MODULE