FastQCFastQC Report
Fri 17 Jun 2016
SRR1526285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48684
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC790.16227097198258156No Hit
GTATACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC640.13146002793525594No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC600.12324377618930245No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC560.11502752444334893No Hit
GTATACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT490.10064908388793034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC150.00803474950.5381437
TGCTTGT203.9303207E-450.5381437
TTCTCAC150.00803474950.5381438
GCTTGTC203.9303207E-450.5381438
TTATTCA150.00806803750.4853940
TATTCAG150.00806803750.4853941
TGTCGAC203.9506695E-450.48538241
ATTGCCT150.00820222150.27546735
ACCAAGC150.008236024550.22326348
TGATACA252.0040226E-550.22326334
GCCGGAC150.008236024550.22326334
GCTAACT150.008236024550.22326349
TACCAAG150.008236024550.22326347
CTACCAA150.008236024550.22326346
CCTACCA150.00826993250.1711645
CCAATTC150.00833805750.0672943
CTAACTT150.00833805750.0672950
GATTAAG150.00833805750.0672950
CATATGC301.0174954E-650.0672933
GACTCAA150.00833805750.0672933