SRR1526284_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92435
Sequences flagged as poor quality	0
Sequence length	56
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	521	0.5636393141126197	No Hit
GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	507	0.548493535998269	No Hit
GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	493	0.5333477578839184	No Hit
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	484	0.5236111862389787	No Hit
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	468	0.5063017255368637	No Hit
GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	449	0.48574674095310216	No Hit
GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	415	0.44896413696110776	No Hit
GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	406	0.43922756531616813	No Hit
GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	399	0.4316546762589928	No Hit
GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	394	0.4262454697895819	No Hit
GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	383	0.4143452155568778	No Hit
GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	352	0.38080813544653	No Hit
GAATTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG	221	0.23908692594796344	No Hit
GAATTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG	177	0.19148590901714718	No Hit
GAATTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG	151	0.1633580353762103	No Hit
GAATTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG	142	0.1536214637312706	No Hit
GAATTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	133	0.14388489208633093	No Hit
GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	111	0.12008438362092282	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	109	0.11792070103315844	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	105	0.1135933358576297	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCACA	20	3.986513E-4	50.442196	39
TAGCCAC	20	3.986513E-4	50.442196	38
TACGCCT	20	3.986513E-4	50.442196	38
TACGCCA	20	3.986513E-4	50.442196	38
TACGACC	20	3.986513E-4	50.442196	38
ACGACCA	20	3.986513E-4	50.442196	39
ACAGTAG	15	0.00811879	50.442192	40
TCACCGG	15	0.00811879	50.442192	40
CGACCAG	45	1.2187229E-10	50.442192	40
GCCTCAA	30	9.761025E-7	50.442192	39
TCTGGCC	15	0.00811879	50.442192	38
AGAGATC	15	0.00811879	50.442192	38
TCCGACC	35	4.8681613E-8	50.44219	38
GCACCGT	25	1.9670928E-5	50.44219	40
GTACGAC	20	3.9973145E-4	50.41457	37
AAGAGAT	15	0.008136401	50.414566	37
CTTCAAT	15	0.008136401	50.414566	41
AAACCGG	15	0.008136401	50.414566	37
GTCCGAC	25	1.973486E-5	50.414566	37
TCACAGT	35	4.8892616E-8	50.414562	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content