##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526284_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 92435 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.42543408881917 39.0 37.0 40.0 33.0 40.0 2 36.04568615784064 38.0 35.0 39.0 30.0 40.0 3 35.99056634391735 38.0 36.0 40.0 28.0 40.0 4 36.837204522096606 39.0 36.0 40.0 31.0 40.0 5 36.968972791691456 39.0 37.0 40.0 32.0 40.0 6 34.394872072267 38.0 35.0 40.0 18.0 40.0 7 36.511061827229945 38.0 36.0 40.0 30.0 40.0 8 36.37090928760751 38.0 35.0 40.0 30.0 40.0 9 36.38023475956077 38.0 36.0 40.0 30.0 40.0 10 35.93193054578893 38.0 35.0 40.0 29.0 40.0 11 34.6743765889544 38.0 33.0 39.0 26.0 40.0 12 32.470763239032834 36.0 29.0 39.0 17.0 40.0 13 31.872807919078273 36.0 28.0 39.0 15.0 40.0 14 31.32948558446476 36.0 27.0 39.0 13.0 40.0 15 32.10424622707849 36.0 29.0 39.0 16.0 40.0 16 32.47254827716774 36.0 30.0 39.0 17.0 40.0 17 32.73363985503327 36.0 30.0 39.0 18.0 40.0 18 32.856764212689995 36.0 30.0 39.0 19.0 40.0 19 33.63216314166712 37.0 31.0 39.0 23.0 40.0 20 33.76455887921242 37.0 31.0 39.0 24.0 40.0 21 35.32641315519013 38.0 35.0 40.0 27.0 40.0 22 35.04715746200032 38.0 35.0 39.0 26.0 40.0 23 35.099659219992425 38.0 35.0 39.0 27.0 40.0 24 34.79832314599448 38.0 34.0 39.0 25.0 40.0 25 35.08616865905772 38.0 35.0 39.0 27.0 40.0 26 35.40864391193812 38.0 35.0 39.0 28.0 40.0 27 35.47787093633364 38.0 35.0 39.0 28.0 40.0 28 35.137404662735975 38.0 35.0 39.0 28.0 40.0 29 35.17612376264402 38.0 35.0 39.0 27.0 40.0 30 34.89101530805431 38.0 35.0 39.0 27.0 40.0 31 34.92606696597609 38.0 35.0 39.0 27.0 40.0 32 33.652696489425004 38.0 33.0 39.0 19.0 40.0 33 34.88040244496133 38.0 35.0 39.0 27.0 40.0 34 34.71744469086385 38.0 35.0 39.0 26.0 40.0 35 34.78044030940661 38.0 35.0 39.0 27.0 40.0 36 32.58731000162276 35.0 31.0 38.0 23.0 39.0 37 32.36177854708714 35.0 31.0 38.0 22.0 39.0 38 31.148439443933576 35.0 30.0 38.0 16.0 39.0 39 29.738789419592145 34.0 28.0 38.0 6.0 39.0 40 25.908378860821117 32.0 13.0 38.0 4.0 39.0 41 21.70533888678531 25.0 9.0 33.0 4.0 37.0 42 25.48723968193866 30.0 20.0 33.0 4.0 37.0 43 22.05262076053443 27.0 9.0 33.0 4.0 36.0 44 23.013598745064098 28.0 10.0 33.0 4.0 36.0 45 25.436230864932114 30.0 21.0 34.0 4.0 37.0 46 25.883983339644075 31.0 19.0 36.0 4.0 38.0 47 24.944003894628658 30.0 11.0 35.0 4.0 38.0 48 25.169383891383134 30.0 15.0 35.0 4.0 38.0 49 22.511170011359333 28.0 5.0 35.0 4.0 38.0 50 25.048974955374046 30.0 13.0 35.0 4.0 38.0 51 26.5037485800833 33.0 9.0 38.0 4.0 40.0 52 26.35501703900038 33.0 4.0 38.0 4.0 40.0 53 26.05747822794396 32.0 4.0 38.0 4.0 40.0 54 25.078541677935846 32.0 4.0 38.0 4.0 40.0 55 25.32376264402012 32.0 4.0 38.0 4.0 40.0 56 25.28972791691459 33.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9116716579176867 1 2 1.6770221817246451 1 3 2.705629882159286 1 4 0.9273779479302533 1 5 1.5034520747512303 1 6 4.890440690570479 1 7 2.2755899540028466 1 8 2.361434581199063 1 9 2.457576300078749 1 10 1.7296921818895186 1 11 -3.53392069670738 1 12 -6.993002866150498 1 13 -20.903171377596678 1 14 -18.021550943624646 1 15 -12.215049173005475 1 16 -21.826106004306205 1 17 -21.93716754834398 1 18 -20.94194815181696 1 19 -7.629949759573378 1 20 -11.647397914326056 1 21 -9.24366773878706 1 22 -4.08072960770544 1 23 -5.169718650717169 1 24 -4.41314586705834 1 25 -4.554933111319638 1 26 1.451286778808921 1 27 1.066837000661387 1 28 1.0260234633393424 1 29 1.07473062940889 1 30 1.3512991976595288 1 31 -0.14868146398859494 1 32 -12.849750608129387 1 33 -0.7323430145866681 1 34 -0.47342195581072843 1 35 -0.16046693565677117 1 36 2.597353231433569 1 37 3.1904631757697715 1 38 2.924604492476714 1 39 5.142155588440524 1 40 7.505571401795766 1 41 -5.325872165952326 1 42 -8.793501374494989 1 43 -15.10862705244642 1 44 -16.57154579473271 1 45 -17.355654920427394 1 46 -19.52576368737315 1 47 -18.495512643244474 1 48 -18.090270146776053 1 49 -16.000480390185835 1 50 -17.948171318221362 1 51 -19.157180160753594 1 52 -19.315401061948172 1 53 -19.013629841556842 1 54 -18.65866829536823 1 55 -18.290314038833774 1 56 -18.30304377678207 2 1 1.2306841140972935 2 2 1.945986155768054 2 3 2.679897056023684 2 4 1.6582647980831595 2 5 1.692606993823354 2 6 5.284786927610831 2 7 2.4047016120919054 2 8 2.4991238479999893 2 9 2.4445234172563417 2 10 2.2102620334595926 2 11 -3.4079417304957005 2 12 -11.62998354788392 2 13 -13.338913900159518 2 14 -15.19094006870856 2 15 -8.365791322628805 2 16 -4.45063050643196 2 17 -3.570717186592656 2 18 -11.191966798792421 2 19 -3.3469632599836103 2 20 -4.557295728900531 2 21 2.1832734113583854 2 22 2.7554753598488233 2 23 2.40214679270791 2 24 2.5228577131609526 2 25 2.21339686555811 2 26 2.608294312187006 2 27 2.2297369782850183 2 28 1.917348890355079 2 29 2.723645375437087 2 30 2.3422740615366067 2 31 -0.05649081731148442 2 32 -5.736675348936426 2 33 -0.3373254864297408 2 34 -0.25018036559665546 2 35 0.4210203471059657 2 36 3.746827386725581 2 37 4.48956066215748 2 38 4.488787382012031 2 39 7.277234278676971 2 40 9.310971565889151 2 41 4.634745421874928 2 42 -0.13638195926413843 2 43 -4.064172662948398 2 44 -11.416104607293313 2 45 -3.7547001952838137 2 46 -11.632722738615037 2 47 -10.849730589093658 2 48 -9.417711035863842 2 49 -12.436819615963415 2 50 -9.200576778055778 2 51 -9.389819826599792 2 52 -9.423105992208482 2 53 -9.547007927631281 2 54 -15.340774657716256 2 55 -9.246680116256014 2 56 -8.429321094601116 3 1 1.434423951495667 3 2 1.8172056679631794 3 3 2.9119295763488893 3 4 1.911598131416497 3 5 2.112932197075388 3 6 5.389339773139284 3 7 2.624213807213856 3 8 2.9768474252357606 3 9 2.9628974009961766 3 10 2.1899368302075573 3 11 2.0738468873904807 3 12 2.017333525286812 3 13 -5.520052111541631 3 14 -5.321183971147583 3 15 -3.63180758279141 3 16 2.2431906317794237 3 17 2.1052177727569372 3 18 2.0786023068986346 3 19 0.2829554392033842 3 20 0.3661189052458127 3 21 3.173029508919363 3 22 3.6011664167593906 3 23 2.668813459374576 3 24 3.249361778201603 3 25 3.0572993045825 3 26 2.109757726821151 3 27 2.1246963278785103 3 28 1.974096857834752 3 29 2.952100659989931 3 30 1.8777212160081547 3 31 2.0698506461031485 3 32 1.3189344071611373 3 33 1.3934062208873357 3 34 2.3098196344033397 3 35 1.714028477187263 3 36 3.5115428338800463 3 37 4.374438710937966 3 38 4.929925593394142 3 39 7.18129931932738 3 40 9.116825224425735 3 41 8.836696641387121 3 42 6.501666821223665 3 43 -0.1209206304280741 3 44 2.0798303520562804 3 45 5.6241615933340725 3 46 -1.854999161379265 3 47 -5.8189988817765865 3 48 -2.184215100904492 3 49 -10.41974644523171 3 50 -2.5971621439094363 3 51 -3.522990558307111 3 52 -3.0959515206637676 3 53 -3.534975407306078 3 54 -8.222888478854468 3 55 -3.5250540999958524 3 56 -0.3820040214303866 4 1 1.7911110680600828 4 2 2.3573283673496803 4 3 3.700845731768112 4 4 2.8566288062631244 4 5 2.548310520183769 4 6 6.090812165777322 4 7 3.133416261201589 4 8 3.5071541737020198 4 9 3.5852286893397363 4 10 3.2870738445224887 4 11 4.664480834220747 4 12 5.3125073494176895 4 13 5.882892673734439 4 14 5.222497010447508 4 15 6.6073744212985694 4 16 6.710225396605598 4 17 6.231966239014604 4 18 6.264614576837285 4 19 4.629294907506043 4 20 5.149472688476891 4 21 3.762354662293596 4 22 4.194499750092724 4 23 3.6945803305402194 4 24 4.1972554387333005 4 25 3.8908371368933388 4 26 2.898632982444873 4 27 2.62072904771491 4 28 2.6036674099819948 4 29 3.0537366518099702 4 30 2.81845741232717 4 31 3.3475193577595945 4 32 4.253249335586489 4 33 3.050175137042757 4 34 3.4962409022970036 4 35 3.1542738759602713 4 36 4.888679848194979 4 37 5.568467340794818 4 38 5.199496145541385 4 39 7.66064492259936 4 40 9.660465306225323 4 41 5.216819340773625 4 42 8.635327352921003 4 43 6.721901455461499 4 44 10.202529738559349 4 45 7.491646255910759 4 46 7.373262597311943 4 47 7.173761854419734 4 48 7.157379991120049 4 49 5.358781162130256 4 50 7.624801046274612 4 51 5.005312100179598 4 52 4.692760135777952 4 53 4.837948928072244 4 54 -0.956017313210296 4 55 5.181121769124804 4 56 4.582699455052232 5 1 2.5109756756336026 5 2 3.1247918748597314 5 3 4.2583663579580815 5 4 3.0398739934854575 5 5 2.9692540361558457 5 6 6.610719083484113 5 7 3.5000758761793733 5 8 3.7327094942012806 5 9 3.842897400996179 5 10 3.747408094575377 5 11 5.387180220723813 5 12 7.78997720344773 5 13 7.764085819492848 5 14 7.9153677529903455 5 15 8.012790118358016 5 16 7.509167643273674 5 17 7.442459152067279 5 18 7.0289471344848415 5 19 6.10456463460568 5 20 6.0279579857055765 5 21 4.165443302022808 5 22 4.491051474230659 5 23 4.365365183512509 5 24 4.692580169006199 5 25 4.220517695387102 5 26 3.5819416348671282 5 27 3.698029661211848 5 28 3.5623727199037205 5 29 3.6291121542428044 5 30 3.8025488022150498 5 31 3.889850646103149 5 32 4.792957395666882 5 33 3.4357050714620456 5 34 3.7268311286562152 5 35 3.537476753049333 5 36 4.886945132730627 5 37 4.699036412087388 5 38 5.537511800290698 5 39 7.176701618177951 5 40 6.989468902586651 5 41 0.846351813800915 5 42 9.070402453407574 5 43 11.814941438537446 5 44 10.907416558952832 5 45 8.65864435195476 5 46 9.729598539770159 5 47 9.789966635464793 5 48 9.106819381854127 5 49 10.190598382354498 5 50 9.738699925056082 5 51 8.773791050888292 5 52 8.817151927612098 5 53 9.254449880050245 5 54 9.084008072869668 5 55 9.573336704601846 5 56 8.779145403856973 6 1 1.9825398935246525 6 2 2.1580752331805684 6 3 3.2145382720010645 6 4 2.506090885039683 6 5 2.47380176229278 6 6 5.815716584733487 6 7 3.3053732275037078 6 8 3.4582967005980834 6 9 3.4081147923005304 6 10 2.805878859193065 6 11 5.171238191738304 6 12 8.019362510794057 6 13 6.492121801501845 6 14 8.440555159287197 6 15 7.160366330252071 6 16 7.206668892648988 6 17 6.9686960336265 6 18 6.233095060521823 6 19 5.602665584130918 6 20 5.195973977709578 6 21 3.5828845813831265 6 22 4.136528735599974 6 23 3.3441757782151527 6 24 4.567042937621892 6 25 3.5370094495100375 6 26 3.0584533789950683 6 27 3.0487542988930016 6 28 2.7575751187043167 6 29 3.5636948628884824 6 30 3.3330835348487327 6 31 3.082604269291558 6 32 4.014296726001717 6 33 2.2319569455250203 6 34 3.046921083678704 6 35 2.813738622114805 6 36 3.1176297904017893 6 37 3.1929894355756474 6 38 3.788186462959363 6 39 5.31753120338535 6 40 2.4521875432663123 6 41 -0.5621439383230218 6 42 7.625724792238156 6 43 10.428934442035697 6 44 9.942149192635991 6 45 6.119813767247116 6 46 10.239493592243925 6 47 10.299261987788633 6 48 10.675784899095508 6 49 11.988949206942205 6 50 9.907765392322446 6 51 9.399328282272599 6 52 9.038541232959421 6 53 9.407923143418557 6 54 9.958270941435384 6 55 8.99262705942444 6 56 9.37944525393193 7 1 2.1636993138145115 7 2 2.8030027694124513 7 3 3.9246831995372915 7 4 2.897395232865769 7 5 2.7709032115681396 7 6 6.366441222414643 7 7 3.5300109086631295 7 8 3.443803946974896 7 9 3.7269553720106714 7 10 3.1392121925264007 7 11 5.250948336665843 7 12 7.186029177460721 7 13 7.600817453675759 7 14 8.49127979696836 7 15 7.711090967933227 7 16 7.48927758830116 7 17 7.004927917684476 7 18 6.798312451826174 7 19 5.769332250797582 7 20 5.355394267564648 7 21 3.742304871238204 7 22 3.92638380806374 7 23 3.6050453434325433 7 24 4.067042937621892 7 25 3.805125391539022 7 26 2.891786712328397 7 27 3.113971690197353 7 28 3.0184446839217074 7 29 3.2086223991203653 7 30 2.4055473029646777 7 31 3.1333289069727144 7 32 4.651977885422006 7 33 3.2536960759598017 7 34 3.590399344548267 7 35 2.5021444192162505 7 36 2.052412399097438 7 37 1.7147285660104288 7 38 2.1939835644086365 7 39 5.063908014979553 7 40 -1.2217255002119494 7 41 -9.35199901078679 7 42 5.756159574846855 7 43 9.28400690580381 7 44 8.927656439012804 7 45 5.6632920281166825 7 46 7.601812432823632 7 47 8.487667784890082 7 48 8.05984287011 7 49 10.488949206942205 7 50 7.871533508264477 7 51 9.247154369229122 7 52 9.806657174988406 7 53 9.864444882548995 7 54 10.71189412984118 7 55 9.985380682612846 7 56 9.647561195960922 8 1 1.574718069142726 8 2 2.4560291973749457 8 3 3.574086439093989 8 4 2.587186366710611 8 5 2.2987165108008796 8 6 5.7016927143157545 8 7 3.013919689566798 8 8 3.45978860170635 8 9 3.489956224525592 8 10 3.118858398835009 8 11 4.493062573664986 8 12 7.329784505678969 8 13 7.148771417870137 8 14 8.20558073473477 8 15 6.9395649662282 8 16 6.95485729844609 8 17 6.7634530668745825 8 18 5.858308189251577 8 19 5.613641713713186 8 20 5.190432630736005 8 21 3.0136177442134766 8 22 4.047440926563311 8 23 3.2820487534922194 8 24 4.147499033103564 8 25 3.153377735955054 8 26 2.4399538052525216 8 27 2.453912014153019 8 28 1.515567446070051 8 29 2.3967085031271864 8 30 2.3886035689493283 8 31 2.1119094696325647 8 32 3.6899147993179966 8 33 2.181445436573611 8 34 1.8135451245994219 8 35 2.028146124246092 8 36 0.4925232260369121 8 37 0.37287008348698336 8 38 0.4422785345706153 8 39 2.3131620644254163 8 40 -0.41631203047622733 8 41 -9.603597476259935 8 42 4.7906864290668025 8 43 7.690549957807221 8 44 6.866595057938632 8 45 4.27710276980466 8 46 7.60490279940505 8 47 7.91658935351753 8 48 7.1831378402719785 8 49 10.049371201827114 8 50 7.498131973737621 8 51 8.451225127967398 8 52 8.332872008748001 8 53 8.908455965242943 8 54 8.953582109380825 8 55 9.153965507847285 8 56 9.091721468765694 9 1 2.105048951495668 9 2 2.6628306679631777 9 3 3.648075409682221 9 4 2.780660631416495 9 5 2.6842863637420535 9 6 6.1865272731392835 9 7 3.241401307213856 9 8 3.427159925235763 9 9 3.514772400996179 9 10 2.687332663540893 9 11 4.787180220723812 9 12 7.277854358620143 9 13 7.129010388458369 9 14 8.325066028852415 9 15 7.729546583875258 9 16 7.33766979844609 9 17 6.1752177727569375 9 18 6.538914806898635 9 19 5.668788772536715 9 20 4.939973071912476 9 21 3.7149045089193606 9 22 3.9210622500927244 9 23 3.6860009593745744 9 24 4.517903444868267 9 25 4.17084097124917 9 26 2.9679868934878186 9 27 2.919904661211845 9 28 2.745346857834754 9 29 3.1922048266565994 9 30 2.7764712160081544 9 31 3.0507881461031516 9 32 4.798830240494468 9 33 3.065177054220669 9 34 3.393048801070009 9 35 2.944965977187266 9 36 1.8358136672133831 9 37 1.6642303776046319 9 38 2.7708630933941443 9 39 4.45057015266071 9 40 -1.9820289422409338 9 41 -4.313615858612877 9 42 5.4473959878903315 9 43 8.459391869571927 9 44 7.65795535205628 9 45 5.510099093334073 9 46 8.680730005287401 9 47 8.985063618223414 9 48 9.050784899095508 9 49 11.669316054768291 9 50 8.747212856090563 9 51 9.557842775026224 9 52 9.469360979336233 9 53 9.395128759360588 9 54 9.566174021145532 9 55 10.248175066670814 9 56 8.827475145236281 10 1 1.956611451495668 10 2 2.1940806679631777 10 3 3.241825409682221 10 4 2.061910631416495 10 5 2.2702238637420535 10 6 5.8505897731392835 10 7 3.030463807213856 10 8 3.224034925235763 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114 55 -2.4212445761863286 114 56 1.2160912166648536 115 1 0.9390616326550898 115 2 2.1580752331805684 115 3 2.439175953160479 115 4 1.6292792908367844 115 5 1.2129321970753892 115 6 4.5693397731392835 115 7 2.1749384448950124 115 8 2.4945285846560523 115 9 2.2921727633150226 115 10 1.950806395424948 115 11 -0.89397919956604 115 12 -6.3574490834088415 115 13 -2.4499071840054008 115 14 -8.508720203031643 115 15 -2.4845612059798157 115 16 -12.075939803003184 115 17 -11.08927498086625 115 18 -2.5422672583187556 115 19 -3.6944358651444453 115 20 -3.253301384609262 115 21 1.749551248049798 115 22 1.5713113442956228 115 23 1.7354801260412387 115 24 2.248202357911744 115 25 1.732661623423084 115 26 1.5584533789950683 115 27 1.4255658930959 115 28 1.2938070027622928 115 29 2.0926803701348575 115 30 1.8403299116603264 115 31 1.6985462982770656 115 32 -4.688601824722923 115 33 1.5870294092931303 115 34 0.9237326778816026 115 35 1.060115433709008 115 36 3.27705008025686 115 37 3.2509604500683977 115 38 4.947606752814433 115 39 6.578400768602741 115 40 9.024651311382254 115 41 8.61176910515524 115 42 3.4083334878903315 115 43 2.0231373405864197 115 44 -0.514372546494446 115 45 2.7719876802905965 115 46 -3.202535393263325 115 47 -8.142766997718615 115 48 -4.447403506701594 115 49 -10.221195720594027 115 50 -1.4617998250688586 115 51 0.855850021403036 115 52 0.9805702184666671 115 53 0.09632894052001006 115 54 0.3785607965078519 115 55 1.0650908275403808 115 56 3.3359669930623674 116 1 0.9168049038766171 116 2 2.1472056679631777 116 3 3.062881957301272 116 4 1.682074321892685 116 5 1.3081702923134841 116 6 4.497911201710714 116 7 2.278975711975761 116 8 2.5830379014262377 116 9 2.378611686710464 116 10 1.5589844492551777 116 11 0.4062278397714323 116 12 0.31542876338204806 116 13 -3.3743378258273466 116 14 -7.73594587590949 116 15 -3.9799028208866467 116 16 -10.003476034887242 116 17 -11.158115560576395 116 18 -4.1385405502442225 116 19 -3.8937112274632852 116 20 -4.724833475706571 116 21 0.7335056993955504 116 22 1.0397378453308193 116 23 1.0926229831841 116 24 0.4174570162968365 116 25 1.076346923630119 116 26 1.3069005839640084 116 27 1.1289820421642247 116 28 1.964096857834754 116 29 1.8116244695137453 116 30 1.2481974064843442 116 31 -1.1125303062777974 116 32 -10.41582749760077 116 33 -0.1546890172079003 116 34 -0.34541846083475036 116 35 -0.968352475193683 116 36 1.5296380719752882 116 37 2.056343472842727 116 38 2.4646874981560494 116 39 4.507489795517856 116 40 6.545872843473351 116 41 5.2686014032918855 116 42 1.7297620593189045 116 43 -10.194253963761406 116 44 -2.4915982193722925 116 45 -0.8972669780944997 116 46 -5.703570589950694 116 47 -10.798046500824205 116 48 -7.395643672333064 116 49 -6.393555969041232 116 50 -5.3681145248618165 116 51 -4.022514367830919 116 52 -4.214046758759004 116 53 -5.890213502544174 116 54 -11.068126574092563 116 55 -6.240292195233948 116 56 -3.936289735716098 117 1 0.0038976357061955014 117 2 1.8445740890158078 117 3 1.3705260675769608 117 4 1.0179139208901802 117 5 1.0374936005841562 117 6 2.437760825770866 117 7 1.018950649319116 117 8 1.2515842673410233 117 9 1.3165816115224942 117 10 0.7864280582777354 117 11 -10.107556621381452 117 12 -11.29985945716933 117 13 -9.58110474312058 117 14 -9.0548681816739 117 15 -5.430404074019478 117 16 -13.11751112260654 117 17 -10.601098016716747 117 18 -3.939292429943471 117 19 -3.3805533327264428 117 20 -3.03247758598226 117 21 -0.04907575423853672 117 22 0.08923659219798452 117 23 -0.746976014309638 117 24 -4.535550502500154 117 25 -0.06024455506661752 117 26 0.8269507092772912 117 27 1.2085559770013177 117 28 0.8456758052031716 117 29 2.234556800340812 117 30 1.3735106896923668 117 31 -1.120675669686321 117 32 -16.322469101610796 117 33 -1.1753657089372282 117 34 -1.1098294884036761 117 35 -1.6607083649179941 117 36 -0.9277554117339868 117 37 -0.6967893592374743 117 38 -0.2163901960795407 117 39 2.06200107371334 117 40 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118 23 0.42702241459845425 118 24 -4.926359614833224 118 25 0.9597868667715588 118 26 0.8253796173684123 118 27 1.4234027955402055 118 28 0.7588729772377363 118 29 1.7098121027760058 118 30 1.520407783172331 118 31 -0.5668657718072936 118 32 -12.27847852816225 118 33 -0.13176790846589626 118 34 0.05827734584612898 118 35 -0.1838819705739283 118 36 3.0427866149745775 118 37 3.080508362679261 118 38 3.3696270859314588 118 39 6.04000578698907 118 40 8.487770498057575 118 41 7.652219029446826 118 42 0.1695275177410771 118 43 1.8554972800196872 118 44 2.064904978921952 118 45 -0.06837572009876425 118 46 -5.115795181279761 118 47 -9.708849628045243 118 48 -8.107797190456731 118 49 -5.701835997470514 118 50 -5.901341248387048 118 51 -6.935627374227508 118 52 -7.22968286394735 118 53 -7.64323411377374 118 54 -12.722888478854468 118 55 -8.694805343776947 118 56 -6.279217951778644 119 1 0.8370654609296295 119 2 2.1236207623027994 119 3 2.4728100794935415 119 4 1.5765037917938542 119 5 1.4645045241194126 119 6 4.135377508988341 119 7 1.4921383355157403 119 8 1.7813757271225583 119 9 1.6164823066565575 119 10 0.9100626163710785 119 11 -11.910932986823358 119 12 -10.004427481002498 119 13 -4.23552380965484 119 14 -4.052882084355133 119 15 -8.532688085936062 119 16 -10.87768987136523 119 17 -12.961574680073252 119 18 -4.677624108195705 119 19 -3.8984282085953623 119 20 -4.370610654502617 119 21 -1.9805553967410177 119 22 -1.503613457454442 119 23 -2.1764695594933485 119 24 -7.517179102301544 119 25 -1.385467991014984 119 26 1.7107640161293247 119 27 1.0451994725326017 119 28 0.6952289333064527 119 29 1.3149937417509392 119 30 1.019985366951552 119 31 -1.3629795425760918 119 32 -13.333392636864023 119 33 0.1478716296923679 119 34 -1.4325703027035743 119 35 -0.5772922775297147 119 36 1.7946874879681012 119 37 1.9611053776046319 119 38 2.5740765367903684 119 39 4.737274162094671 119 40 5.9158189351175565 119 41 6.794432490443725 119 42 1.615880657701652 119 43 0.8183246525907961 119 44 0.7968114841317515 119 45 0.8958597065416214 119 46 -5.042294758863541 119 47 -8.90541397611621 119 48 -9.560064157508267 119 49 -8.214086067873218 119 50 -5.718294219381136 119 51 -7.790034583464344 119 52 -7.4287817093430135 119 53 -9.269818174601676 119 54 -11.364397912816733 119 55 -10.374613848423525 119 56 -6.319488298159946 120 1 0.5528854899572053 120 2 1.9279748987324083 120 3 2.542185986605297 120 4 1.4839058237241858 120 5 1.371906556049744 120 6 3.8308782346777477 120 7 1.7780599610600092 120 8 1.9491551175434552 120 9 2.027512785611563 120 10 1.654039394310125 120 11 -12.489742856199264 120 12 -4.650871602918318 120 13 -5.852359803849325 120 14 -8.642722432686046 120 15 -7.03283322381705 120 16 -11.793219624630833 120 17 -10.659397611858449 120 18 -4.881397693101366 120 19 -4.2975573813094385 120 20 -6.673368274241369 120 21 -0.2531243372344889 120 22 0.42526898086195786 120 23 -0.0850326944715789 120 24 -5.7578177089778855 120 25 -1.2914186441354474 120 26 1.6318090088724375 120 27 1.1903373535195385 120 28 1.1140968578347525 120 29 1.4572288651181395 120 30 0.365413523700461 120 31 -2.2024570462045396 120 32 -12.7969630287363 120 33 -1.9101835227024075 120 34 -10.968129083545374 120 35 -3.5044330612742733 120 36 -3.344354602017386 120 37 -3.4850484685492127 120 38 -3.5977667142981637 120 39 -0.42382888580083034 120 40 -1.8821491345486265 120 41 -0.5663802816897991 120 42 -6.6839742044173605 120 43 -3.645536015043458 120 44 -5.120169647943719 120 45 -11.76045379128131 120 46 -16.26987095625106 120 47 -15.555921958699663 120 48 -13.116522793212186 120 49 -15.719746445231708 120 50 -13.106392913140207 120 51 -13.42196491728147 120 52 -13.191336136048381 120 53 -13.859077971408642 120 54 -14.169042325008315 120 55 -13.747618202559956 120 56 -11.255337354763718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 866.0 5 152.0 6 146.0 7 172.0 8 237.0 9 176.0 10 122.0 11 118.0 12 129.0 13 176.0 14 241.0 15 345.0 16 475.0 17 671.0 18 824.0 19 919.0 20 1191.0 21 1427.0 22 1865.0 23 2220.0 24 2515.0 25 2601.0 26 2774.0 27 2911.0 28 3022.0 29 3235.0 30 3616.0 31 4167.0 32 4951.0 33 5996.0 34 7344.0 35 8676.0 36 10230.0 37 10194.0 38 6177.0 39 1554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.69492075512522 0.24990533888678532 0.03461892140423 0.020554984583761562 8 99.64299237301888 0.2650511170011359 0.048682858224698436 0.043273651755287496 9 98.86056225247258 0.7379834224251737 0.1612309823186963 0.24022334278356094 10 39.17671877535566 45.97825498999297 7.1423162222102015 7.702710012441175 11 24.20914834689285 27.26761294789683 34.232733252553224 14.290505452657086 12 49.09936712284308 12.545031643857845 17.364634608102993 20.990966625196084 13 21.703900037864447 26.062638610915776 20.293178990642073 31.940282360577704 14 36.86938643004004 15.55134725679039 19.921004220322473 27.658262092847096 15 21.301845690318526 29.587402052693758 19.250235723807563 29.86051653318016 16 22.690867372340197 17.04547914366734 38.81204407212589 21.45160941186657 17 42.02285368017833 18.533988356742483 19.269807604908348 20.17335035817084 18 41.076355452647064 21.652618936844725 18.368804301959468 18.90222130854874 19 23.41452330198989 40.44385773180259 16.667929060670655 19.473689905536858 20 22.26659307598238 22.421349956170253 17.58276246442215 37.729294503425216 21 20.70584671311724 21.956549963214613 38.438568399186394 18.899034924481757 22 22.370314274895872 21.352301617352733 29.3471087791421 26.930275328609294 23 20.74322496889706 41.001785038134905 21.15216097798453 17.102829014983502 24 20.15686698761292 39.239465570400824 21.24087196408287 19.36279547790339 25 20.313733975225833 38.20955265862498 23.49542922053335 17.981284145615838 26 27.114382024907602 21.206143440890518 34.038217665102266 17.64125686909962 27 35.06541287481198 18.7527728782747 25.843766569638472 20.338047677274844 28 20.125281775619907 36.32737992023583 26.581194728628404 16.966143575515865 29 20.26990465677521 35.367899310628445 28.046708440202156 16.315487592394188 30 18.508257397026036 19.4443843206857 48.31172485444038 13.735633427847882 31 18.512414615634825 19.735443998698905 47.96812317033503 13.784018215331237 32 35.535839870165 16.513930213686773 31.871246956992156 16.078982959156072 33 19.701864700780572 34.06764960971379 32.4490459670425 13.78143972246314 34 16.76736832986833 19.328222453222455 35.75342169092169 28.150987525987524 35 33.00571527537236 21.009049186006234 34.974670938690686 11.010564599930724 36 31.565087346655684 21.934197407776672 34.17464996315401 12.326065282413628 37 31.557141462673943 20.45639418227568 35.48659992841726 12.499864426633117 38 18.216978679147168 22.231972612237826 48.619778124458314 10.9312705841567 39 16.888956468850466 30.618429055521766 30.553262664002084 21.939351811625684 40 9.977899469805013 14.805177838502825 24.772190347620654 50.44473234407151 41 22.48697760256844 15.731711312286373 26.91295469188516 34.86835639326002 42 13.175672012404716 21.707166325103284 36.15583964954512 28.961322012946876 43 8.801937403618513 20.202645468169674 27.563476032232142 43.43194109597967 44 9.345326792764768 15.04475793732801 25.712700812495243 49.897214457411984 45 14.775619848138561 36.108794315486186 35.01803489996858 14.09755093640667 46 29.24059539918809 21.899864682002708 35.512855209742895 13.346684709066306 47 13.590120998290006 18.478754951405875 47.62657200372302 20.304552046581094 48 15.05898906808096 20.59854962658296 51.0477324385756 13.294728866760472 49 10.883866345339673 14.980785514231746 25.22199787229423 48.91335026813435 50 12.566131830008672 20.58434518647008 53.16673894189072 13.68278404163053 51 30.388101829235197 20.508060576453346 36.59990229604299 12.503935298268468 52 13.853707284221503 36.219069757337444 35.10351719493385 14.823705763507203 53 29.507236949097415 22.118501653385376 35.62530492763051 12.748956469886702 54 12.231345870526042 19.741745385215356 45.648453072189966 22.378455672068636 55 14.224086538982647 20.429443197953585 35.80463694598901 29.541833317074754 56 30.555916916005376 20.828454967260743 33.35176271627423 15.26386540045965 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 4.5 7 6.0 8 40.0 9 74.0 10 147.0 11 220.0 12 260.0 13 398.5 14 497.0 15 593.5 16 690.0 17 756.5 18 823.0 19 967.5 20 1112.0 21 1428.0 22 2041.5 23 2339.0 24 2639.5 25 2940.0 26 3284.0 27 3628.0 28 3713.0 29 3637.5 30 3477.0 31 3255.0 32 3033.0 33 2678.5 34 2324.0 35 2125.0 36 1926.0 37 1816.0 38 1726.0 39 1746.0 40 1841.5 41 1937.0 42 2270.0 43 2603.0 44 3688.0 45 4773.0 46 5631.5 47 6227.0 48 5964.0 49 5363.0 50 4762.0 51 4578.0 52 4394.0 53 4394.5 54 4186.5 55 3978.0 56 4025.5 57 4073.0 58 4098.0 59 4123.0 60 3917.5 61 3712.0 62 3326.0 63 2433.0 64 1926.0 65 1692.5 66 1459.0 67 1226.0 68 993.0 69 817.0 70 641.0 71 518.5 72 336.5 73 277.0 74 195.0 75 113.0 76 84.0 77 55.0 78 44.0 79 21.5 80 10.0 81 6.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.022718667171525937 10 0.0 11 0.0032455238816465628 12 0.0 13 0.0 14 0.027046032347054688 15 0.17958565478444313 16 0.044355493049169685 17 0.022718667171525937 18 0.012982095526586251 19 0.019473143289879376 20 0.03461892140423 21 0.007572889057175312 22 0.0 23 0.0 24 0.0 25 0.0 26 0.18824038513550062 27 0.023800508465408127 28 0.17634013090279657 29 0.03461892140423 30 0.03570076269811219 31 0.22177746524584843 32 0.010818412938821874 33 0.21095905230702655 34 0.08979282739222157 35 0.05517390598799156 36 0.1720127657272678 37 0.25315086276843185 38 0.14172120949856656 39 0.39270838967923405 40 0.6296316330394331 41 0.9325471953264455 42 0.22935035430302375 43 0.3818899767404122 44 0.536593281765565 45 0.12332990750256938 46 0.06491047763293126 47 0.04002812787364094 48 0.048682858224698436 49 0.34294369016065346 50 0.21095905230702655 51 0.34618921404229996 52 0.40352680261805596 53 0.2163682587764375 54 0.13306647914750905 55 0.190404067723265 56 0.2066316871314978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 92435.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.81900795153351 #Duplication Level Percentage of deduplicated Percentage of total 1 92.6486125785464 72.09823118948451 2 4.593226936551187 7.148807269973495 3 1.1858421842851583 2.768431871044518 4 0.5491297336373242 1.709309244333856 5 0.2724795640326975 1.0602044680045437 6 0.14041038758827784 0.6555958240926055 7 0.09453372629705833 0.5149564558879213 8 0.09175332258243897 0.571212203169795 9 0.06116888172162598 0.4284091523773462 >10 0.2975031974642718 4.232163141667118 >50 0.0375354501473614 1.8672580732406556 >100 0.025023633431574266 5.833288256612755 >500 0.0027804037146193627 1.1121328501108887 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 521 0.5636393141126197 No Hit GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 507 0.548493535998269 No Hit GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 493 0.5333477578839184 No Hit GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 484 0.5236111862389787 No Hit GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 468 0.5063017255368637 No Hit GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 449 0.48574674095310216 No Hit GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 415 0.44896413696110776 No Hit GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 406 0.43922756531616813 No Hit GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 399 0.4316546762589928 No Hit GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 394 0.4262454697895819 No Hit GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 383 0.4143452155568778 No Hit GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 352 0.38080813544653 No Hit GAATTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 221 0.23908692594796344 No Hit GAATTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 177 0.19148590901714718 No Hit GAATTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 151 0.1633580353762103 No Hit GAATTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 142 0.1536214637312706 No Hit GAATTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 133 0.14388489208633093 No Hit GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 111 0.12008438362092282 No Hit GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 109 0.11792070103315844 No Hit GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 105 0.1135933358576297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.005409206469410938 0.0 0.0 0.0 0.0 11 0.0086547303510575 0.0 0.0 0.0 0.0 12 0.011900254232704062 0.0 0.0 0.0 0.0 13 0.01622761940823281 0.0 0.0 0.0 0.0 14 0.018391301995997186 0.0 0.0 0.0 0.0 15 0.022718667171525937 0.0 0.0 0.0 0.0 16 0.023800508465408123 0.0 0.0 0.0 0.0 17 0.03029155622870125 0.0 0.0 0.0 0.0 18 0.03678260399199437 0.0 0.0 0.0 0.0 19 0.045437334343051874 0.0 0.0 0.0 0.0 20 0.050846540812462815 0.0 0.0 0.0 0.0 21 0.054092064694109376 0.0 0.0 0.0 0.0 22 0.05625574728187375 0.0 0.0 0.0 0.0 23 0.06166495375128469 0.0 0.0 0.0 0.0 24 0.06491047763293124 0.0 0.0 0.0 0.0 25 0.0681560015145778 0.0 0.0 0.0 0.0 26 0.07140152539622438 0.0 0.0 0.0 0.0 27 0.07464704927787094 0.0 0.0 0.0 0.0 28 0.08113809704116406 0.0 0.0 0.0 0.0 29 0.086547303510575 0.0 0.0 0.0 0.0 30 0.08762914480445719 0.0 0.0 0.0 0.0 31 0.08871098609833937 0.0 0.0 0.0 0.0 32 0.08871098609833937 0.0 0.0 0.0 0.0 33 0.08979282739222157 0.0 0.0 0.0 0.0 34 0.08979282739222157 0.0 0.0 0.0 0.0 35 0.08979282739222157 0.0 0.0 0.0 0.0 36 0.09087466868610375 0.0 0.0 0.0 0.0 37 0.09087466868610375 0.0 0.0 0.0 0.0 38 0.09087466868610375 0.0 0.0 0.0 0.0 39 0.09195650997998593 0.0 0.0 0.0 0.0 40 0.09195650997998593 0.0 0.0 0.0 0.0 41 0.09195650997998593 0.0 0.0 0.0 0.0 42 0.09303835127386813 0.0 0.0 0.0 0.0 43 0.09628387515551469 0.0 0.0 0.0 0.0 44 0.09952939903716125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCACA 20 3.986513E-4 50.442196 39 TAGCCAC 20 3.986513E-4 50.442196 38 TACGCCT 20 3.986513E-4 50.442196 38 TACGCCA 20 3.986513E-4 50.442196 38 TACGACC 20 3.986513E-4 50.442196 38 ACGACCA 20 3.986513E-4 50.442196 39 ACAGTAG 15 0.00811879 50.442192 40 TCACCGG 15 0.00811879 50.442192 40 CGACCAG 45 1.2187229E-10 50.442192 40 GCCTCAA 30 9.761025E-7 50.442192 39 TCTGGCC 15 0.00811879 50.442192 38 AGAGATC 15 0.00811879 50.442192 38 TCCGACC 35 4.8681613E-8 50.44219 38 GCACCGT 25 1.9670928E-5 50.44219 40 GTACGAC 20 3.9973145E-4 50.41457 37 AAGAGAT 15 0.008136401 50.414566 37 CTTCAAT 15 0.008136401 50.414566 41 AAACCGG 15 0.008136401 50.414566 37 GTCCGAC 25 1.973486E-5 50.414566 37 TCACAGT 35 4.8892616E-8 50.414562 41 >>END_MODULE