FastQCFastQC Report
Fri 17 Jun 2016
SRR1526283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39015
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC580.14866077149814175No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC530.13584518774830193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAA150.008060303550.47854240
ACCAACC203.943479E-450.47854238
AACAAAC150.00814364450.347637
ACCAGCC150.00818555350.2823842
TGCAAGA150.00822762350.21733547
CCTGCAC150.00822762350.21733536
CCGTCTC150.00822762350.21733543
CAAGGAG150.00822762350.21733549
CCAAGGA150.00822762350.21733548
TGGCTCT150.00822762350.21733546
GTGCGTA150.00826985450.15245444
CGACCGT150.00826985450.15245444
CGACTCC150.00826985450.15245444
CGTCTCC150.00826985450.15245444
TCTGTGA204.0980466E-450.0877445
ATCTCGT150.00831224650.08773833
TGCGTAC150.00831224650.08773845
ACCCGTC150.00831224650.08773850
GCTGCAA150.00831224650.08773845
GACTCCT150.00831224650.08773845
Produced by FastQC (version 0.11.5)