##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526283_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39015 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.872997565039086 38.0 37.0 39.0 33.0 40.0 2 36.219607843137254 38.0 37.0 39.0 31.0 40.0 3 36.214763552479816 38.0 37.0 39.0 31.0 40.0 4 36.19013200051263 38.0 38.0 39.0 30.0 40.0 5 36.02970652313213 38.0 36.0 39.0 30.0 40.0 6 35.77977700884275 38.0 36.0 39.0 30.0 40.0 7 36.12233756247597 38.0 36.0 39.0 31.0 40.0 8 35.87527873894656 38.0 36.0 39.0 30.0 40.0 9 35.90229398949122 38.0 36.0 39.0 30.0 40.0 10 35.37846981930027 38.0 35.0 39.0 29.0 40.0 11 33.12210688196847 35.0 31.0 38.0 24.0 40.0 12 31.645213379469435 35.0 29.0 38.0 15.0 40.0 13 31.34563629373318 35.0 28.0 38.0 13.0 40.0 14 31.16316801230296 36.0 28.0 38.0 12.0 40.0 15 31.617249775727284 36.0 29.0 38.0 14.0 40.0 16 32.11880046136101 36.0 30.0 38.0 16.0 40.0 17 32.17939254133026 36.0 30.0 38.0 17.0 40.0 18 32.29234909650135 36.0 30.0 38.0 18.0 40.0 19 33.12367038318595 36.0 31.0 38.0 23.0 40.0 20 33.27627835447905 36.0 31.0 38.0 23.0 40.0 21 34.67858515955402 38.0 35.0 39.0 26.0 40.0 22 34.43780597206203 38.0 34.0 39.0 25.0 40.0 23 34.5437395873382 38.0 34.0 39.0 26.0 40.0 24 34.213097526592335 38.0 33.0 39.0 24.0 40.0 25 34.42247853389722 38.0 33.0 39.0 25.0 40.0 26 34.746712802768165 38.0 35.0 39.0 27.0 40.0 27 34.66515442778419 38.0 35.0 39.0 26.0 40.0 28 34.636831987697036 38.0 35.0 39.0 27.0 40.0 29 34.478200692041526 38.0 35.0 39.0 26.0 40.0 30 34.33753684480328 38.0 34.0 39.0 25.0 40.0 31 34.04985262078688 38.0 33.0 39.0 24.0 40.0 32 32.96952454184288 38.0 33.0 39.0 16.0 40.0 33 34.128540305010894 38.0 34.0 39.0 24.0 40.0 34 33.83060361399462 38.0 33.0 39.0 23.0 40.0 35 33.84198385236448 38.0 33.0 39.0 23.0 40.0 36 31.839882096629502 35.0 31.0 38.0 17.0 39.0 37 31.590670255030115 35.0 31.0 38.0 14.0 39.0 38 30.909906446238626 35.0 30.0 38.0 4.0 39.0 39 28.841983852364475 33.0 26.0 38.0 4.0 39.0 40 24.633448673587083 30.0 10.0 37.0 4.0 39.0 41 21.29814174035627 24.0 8.0 33.0 4.0 37.0 42 25.226784570037164 30.0 19.0 34.0 4.0 37.0 43 21.421889017044727 26.0 7.0 33.0 4.0 37.0 44 22.204100986799947 27.0 8.0 33.0 4.0 36.0 45 24.82235037805972 30.0 17.0 34.0 4.0 37.0 46 24.835704216327052 30.0 10.0 36.0 4.0 38.0 47 24.130821478918364 30.0 4.0 36.0 4.0 38.0 48 24.02235037805972 29.0 4.0 35.0 4.0 38.0 49 21.43770344739203 25.0 4.0 35.0 4.0 38.0 50 23.79905164680251 29.0 4.0 35.0 4.0 38.0 51 25.325259515570934 32.0 4.0 38.0 4.0 39.0 52 25.106189926951174 32.0 4.0 38.0 4.0 39.0 53 24.677175445341536 31.0 4.0 38.0 4.0 39.0 54 23.790644623862615 31.0 4.0 37.0 4.0 39.0 55 24.02955273612713 31.0 4.0 37.0 4.0 39.0 56 22.902729719338716 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4176340832195038 1 2 2.3429811289569002 1 3 1.8966732359819503 1 4 2.522298858192393 1 5 2.5994373568076554 1 6 2.7252862600492023 1 7 2.435449115388323 1 8 2.476072819726859 1 9 2.483484222148249 1 10 2.3633026996426096 1 11 -4.889009796680742 1 12 -6.367148471963301 1 13 -21.282334753017302 1 14 -18.60061951487178 1 15 -13.116733599823188 1 16 -20.88483800026281 1 17 -20.850660380868145 1 18 -20.16417009905079 1 19 -5.482671295110176 1 20 -9.699048239030994 1 21 -7.253159565475833 1 22 -2.8915135499399867 1 23 -3.9208216820222397 1 24 -4.039475141328399 1 25 -4.128094650587215 1 26 2.7081793973667345 1 27 2.190360614941291 1 28 2.858770729171617 1 29 1.885909985775669 1 30 3.1783851659134825 1 31 1.2569463541231514 1 32 -11.15089224386423 1 33 0.3860381592869544 1 34 1.31774275459847 1 35 0.766382758085534 1 36 4.357657972231685 1 37 4.3184055407039175 1 38 5.4645099888112085 1 39 7.330401282124416 1 40 9.173550362397354 1 41 -0.6987497487716539 1 42 -5.225643955412046 1 43 -11.902655913481642 1 44 -14.412363728477448 1 45 -10.702343952437062 1 46 -17.12192341189158 1 47 -16.760587160223693 1 48 -14.823272565796248 1 49 -11.932647154421952 1 50 -14.453859650497911 1 51 -15.809739965627127 1 52 -15.501225951548106 1 53 -15.268733467180727 1 54 -17.012686850917945 1 55 -14.576123492276654 1 56 -13.283041559652549 2 1 1.3309674165528378 2 2 1.927743033718805 2 3 1.6223875216962327 2 4 1.95563219152573 2 5 2.296580213950513 2 6 3.0719529267158663 2 7 1.8992586391978463 2 8 2.521787105441142 2 9 2.0577699364339637 2 10 2.336636032975946 2 11 -3.2804383681093157 2 12 -9.102386567201396 2 13 -13.486144276826824 2 14 -15.080619514871781 2 15 -7.1843526474422355 2 16 -3.8381713335961436 2 17 -2.030660380868145 2 18 -9.801312956193648 2 19 -2.237909390348271 2 20 -2.598095858078615 2 21 2.212554720238451 2 22 3.009438831012396 2 23 2.3858449846444287 2 24 2.4786200967668393 2 25 1.6166672541746934 2 26 2.6300841592714903 2 27 2.5636939482746257 2 28 2.541627872028762 2 29 2.590671890537571 2 30 1.712670880199198 2 31 -1.8278155506387535 2 32 -9.136606529578515 2 33 -0.8815808883320955 2 34 -0.6051143882586771 2 35 -0.1193315276287521 2 36 4.6500389246126375 2 37 4.211738874037255 2 38 4.352129036430263 2 39 7.489448901172036 2 40 9.525931314778301 2 41 7.753631203609299 2 42 2.246736996968906 2 43 -3.5007511515768783 2 44 -9.458078014191733 2 45 -0.28424871434182464 2 46 -9.144780554748722 2 47 -9.111063350699883 2 48 -6.186129708653391 2 49 -10.661218582993381 2 50 -5.763383460021721 2 51 -5.022120918008081 2 52 -6.717416427738582 2 53 -6.098257276704537 2 54 -13.037448755679849 2 55 -5.093266349419512 2 56 -4.317327273938265 3 1 1.3479823419259773 3 2 2.225354974017314 3 3 1.5182084172186237 3 4 2.169065027346626 3 5 2.1804608109654353 3 6 2.541206658059153 3 7 2.6906019227799405 3 8 2.8737274039486067 3 9 2.9888147125533706 3 10 2.1294718538714648 3 11 0.8828452139802394 3 12 1.3286582089180072 3 13 -5.858383082796973 3 14 -5.391664290991184 3 15 -1.9777854832631299 3 16 3.244515233568034 3 17 3.201578425102003 3 18 2.971224357239187 3 19 2.437015982786054 3 20 2.1072772762497465 3 21 3.875838302328006 3 22 4.01928957728105 3 23 3.5368897607638345 3 24 3.9001126340802728 3 25 3.657264269100061 3 26 3.246203562256568 3 27 3.822201410961192 3 28 3.6658069765063743 3 29 3.671268905462945 3 30 2.9672977458708374 3 31 3.6059157926448293 3 32 3.14548302266029 3 33 3.5921504549514864 3 34 2.9584677012935643 3 35 3.0329072783413977 3 36 4.063770267896217 3 37 4.912932903888002 3 38 5.668845454340705 3 39 7.837508602664574 3 40 11.267722359554423 3 41 10.38049687525109 3 42 8.00285639995398 3 43 3.2696966096171494 3 44 4.466101090285878 3 45 8.326796061777578 3 46 1.7053686989826211 3 47 -2.764496186520777 3 48 2.190586709257058 3 49 -9.05524843373965 3 50 1.0948254952021585 3 51 0.32743132079789206 3 52 0.5673596916644037 3 53 -0.4663169781970744 3 54 -5.357747263142535 3 55 -1.2541618718075718 3 56 2.7265533230766614 4 1 1.8524166919151597 4 2 2.71875752647243 4 3 2.4070252028556567 4 4 2.9715742205112363 4 5 2.797159924095439 4 6 3.1455761151216635 4 7 3.234620958038427 4 8 3.392221888049839 4 9 3.2702337045499092 4 10 3.2908389315266717 4 11 5.177242791310974 4 12 4.697033722653675 4 13 6.143131085492019 4 14 3.3848877315050316 4 15 7.422603874296897 4 16 6.999219970751685 4 17 6.547890343769534 4 18 5.971440666994759 4 19 4.900351479216948 4 20 5.834078054964863 4 21 4.4890764593688885 4 22 4.6850910049254395 4 23 3.934250781745881 4 24 4.053692560534955 4 25 4.3273918918558465 4 26 2.991823289706275 4 27 3.1034040932021654 4 28 3.7045264227534034 4 29 3.3848747890883004 4 30 3.2260042135325335 4 31 4.482619231969942 4 32 4.708321006653367 4 33 3.9036365029722546 4 34 3.7183638726108867 4 35 4.437190211501687 4 36 5.665111388380751 4 37 5.90536206244305 4 38 6.504882659618666 4 39 9.246550350447396 4 40 9.872308126372506 4 41 4.492761638391908 4 42 8.02383844624427 4 43 8.199828558568047 4 44 11.73670459450392 4 45 8.898070126237887 4 46 10.546813648149826 4 47 10.099661286981277 4 48 8.98314565366545 4 49 6.124288663383432 4 50 10.06994987331161 4 51 8.484255893586123 4 52 9.015337195449824 4 53 7.472467360976623 4 54 0.9593628385230488 4 55 8.856588723044258 4 56 9.183832146351591 5 1 2.2591641378643175 5 2 3.04282500093192 5 3 3.043043259401152 5 4 3.052353503001136 5 5 3.2024818532947705 5 6 3.6542480086830764 5 7 3.516635688378173 5 8 3.2945739906870486 5 9 2.853507641351996 5 10 3.2704065247792258 5 11 5.654315730251341 5 12 8.176957695093687 5 13 8.41516719858301 5 14 7.776429665456085 5 15 8.335647352557764 5 16 7.453959813944838 5 17 7.09196256995153 5 18 6.83934278151127 5 19 5.766352904733697 5 20 6.116330371429584 5 21 4.647308818599107 5 22 4.940258503143539 5 23 4.221254820710001 5 24 5.100259441029131 5 25 4.268470532863212 5 26 4.142215306812474 5 27 4.556480833520531 5 28 4.419660658914012 5 29 4.216901398734294 5 30 4.909064322822147 5 31 4.4013647772301 5 32 5.448639372060828 5 33 4.830222390356425 5 34 5.527344628134763 5 35 5.15279961991223 5 36 6.514956957399519 5 37 7.036984775676597 5 38 7.815407724954852 5 39 8.826498081499903 5 40 9.806259183630761 5 41 4.95035251508471 5 42 10.997228800247594 5 43 15.27728163530837 5 44 13.564217067775477 5 45 11.732472597133583 5 46 13.505711248529966 5 47 13.319428452578805 5 48 12.402722750363004 5 49 12.876486335039406 5 50 12.474321458011069 5 51 11.647715147565691 5 52 12.817665539474532 5 53 12.593873870836443 5 54 12.762879113172609 5 55 13.042143486646061 5 56 12.06004977524206 6 1 1.7509674165528395 6 2 2.6144097003854725 6 3 2.432387521696235 6 4 3.0389655248590586 6 5 2.806580213950511 6 6 3.1919529267158637 6 7 2.875925305864513 6 8 3.261787105441144 6 9 3.1977699364339642 6 10 2.8133026996426125 6 11 5.272894965224019 6 12 7.070946766131936 6 13 7.36052238983984 6 14 7.016047151794886 6 15 7.835647352557764 6 16 7.431828666403856 6 17 7.082672952465185 6 18 6.27868704380635 6 19 5.038757276318396 6 20 5.748570808588056 6 21 3.482554720238454 6 22 4.28943883101239 6 23 2.745844984644428 6 24 4.381953430100168 6 25 3.2433339208413585 6 26 3.086750825938161 6 27 3.007027281607961 6 28 2.701627872028766 6 29 3.6906718905375726 6 30 3.4093375468658635 6 31 2.985517782694579 6 32 4.89672680375482 6 33 3.738419111667902 6 34 4.124885611741327 6 35 3.4640018057045836 6 36 4.4100389246126355 6 37 3.351738874037256 6 38 4.878795703096927 6 39 7.016115567838707 6 40 5.509264648111635 6 41 3.4036312036092973 6 42 7.393403663635574 6 43 12.392582181756456 6 44 10.818588652474933 6 45 8.702417952324843 6 46 10.485219445251278 6 47 10.982269982633447 6 48 10.500536958013274 6 49 11.588781417006619 6 50 10.819949873311614 6 51 8.821212415325252 6 52 8.972583572261417 6 53 8.705076056628798 6 54 9.599217910986816 6 55 9.266733650580488 6 56 8.419339392728403 7 1 2.036681702267124 7 2 2.8906001765759513 7 3 2.932387521696235 7 4 3.194917905811444 7 5 3.492294499664794 7 6 4.016952926715867 7 7 3.762830067769279 7 8 4.190358534012574 7 9 3.7870556507196795 7 10 3.9287788901187994 7 11 5.9324187747478305 7 12 7.68047057565575 7 13 8.390284294601745 7 14 8.042237627985365 7 15 8.54993306684348 7 16 7.478257237832427 7 17 7.560053904846139 7 18 7.139401329520638 7 19 5.9756620382231596 7 20 6.574761284778532 7 21 4.014697577381305 7 22 5.471581688155247 7 23 4.513702127501574 7 24 4.698620096766838 7 25 4.02666725417469 7 26 4.059369873557209 7 27 4.0415510911317725 7 28 4.930199300600194 7 29 3.8442433191090046 7 30 3.4462423087706284 7 31 4.477184449361246 7 32 5.670536327564339 7 33 4.427704825953619 7 34 4.871314183169893 7 35 4.302097043799819 7 36 3.7386103531840646 7 37 4.008881731180107 7 38 4.601414750715975 7 39 5.556591758314891 7 40 -0.6419258280788398 7 41 -9.407083082104988 7 42 6.012451282683191 7 43 11.377105991280263 7 44 10.19477912866541 7 45 7.589322714229603 7 46 10.449505159536994 7 47 11.388222363585832 7 48 11.46244171991804 7 49 12.317352845578046 7 50 10.808045111406848 7 51 12.18430765342049 7 52 11.147583572261418 7 53 12.296742723295463 7 54 12.40397981574872 7 55 12.423876507723342 7 56 9.877672726061736 8 1 2.0316691709388053 8 2 2.8003746126661753 8 3 2.5420366445032556 8 4 2.6626497353853793 8 5 2.8337731964066464 8 6 3.7954616986456884 8 7 3.4855744286715336 8 8 3.3670502633358836 8 9 3.180226076784841 8 10 2.8720746294671713 8 11 4.697456368732787 8 12 7.730595888938954 8 13 7.307890810892477 8 14 6.995871713198397 8 15 7.458454370101627 8 16 6.6063900699126314 8 17 5.807234355973957 8 18 6.110265991174771 8 19 4.843143241230674 8 20 4.9713778261319135 8 21 3.339572264098102 8 22 3.515754620486078 8 23 2.447599370609339 8 24 3.7073920265913998 8 25 3.1100005875080257 8 26 2.908680650499562 8 27 1.9973781588009416 8 28 0.9928559422042014 8 29 3.088917504572663 8 30 1.9979340380939377 8 31 2.8434125195366846 8 32 4.370411014281135 8 33 2.250699813422287 8 34 3.394183857355358 8 35 2.761370226757215 8 36 2.1433722579459697 8 37 2.0289318564933936 8 38 2.977041317132013 8 39 4.572255918715896 8 40 -2.6196827203094166 8 41 -9.106017919197718 8 42 5.4723510320566255 8 43 8.517143585265227 8 44 7.372097424404757 8 45 4.728733741798525 8 46 7.577324708409172 8 47 8.741919105440466 8 48 7.709308887837839 8 49 10.121237557357496 8 50 6.719072680329155 8 51 7.255422941641044 8 52 7.803285326647384 8 53 7.728760267155113 8 54 8.600095103969274 8 55 7.636909089176978 8 56 7.09916395413191 9 1 1.731736647322073 9 2 2.382358418334192 9 3 2.4131567524654685 9 4 3.047939883833422 9 5 2.4950417524120496 9 6 3.2573375421004798 9 7 3.266950946890155 9 8 3.511787105441144 9 9 3.005462244126271 9 10 2.331251417591332 9 11 5.135715478044531 9 12 6.922228817413991 9 13 6.309240338557789 9 14 7.5532266389743725 9 15 7.297185814096224 9 16 6.916444051019244 9 17 6.149339619131855 9 18 6.344071659190966 9 19 5.037475225036342 9 20 5.566519526536773 9 21 2.705631643315371 9 22 3.489438831012393 9 23 3.2650757538751947 9 24 3.1793893275360716 9 25 3.4689749464823834 9 26 3.047007236194574 9 27 2.0621554867361667 9 28 2.9823971027979965 9 29 1.8483641982298806 9 30 3.1234401109684313 9 31 3.313722910899706 9 32 4.101855008883025 9 33 3.18457295782175 9 34 3.5210394578951707 9 35 2.999899241602016 9 36 2.2523466169203274 9 37 2.6325081048064867 9 38 3.3775136518148727 9 39 5.2763719780951135 9 40 1.6836236224706091 9 41 -3.1886764886983947 9 42 7.495967766199676 9 43 11.916941156115428 9 44 10.93653737042365 9 45 7.381905131812022 9 46 10.062142522174359 9 47 11.298936649300117 9 48 10.880024137500456 9 49 13.081089109314313 9 50 10.871231924593665 9 51 11.23787908199192 9 52 10.599506649184498 9 53 11.614050415603156 9 54 11.717166628935537 9 55 11.143656727503569 9 56 10.906518879907889 10 1 1.4372419263567622 10 2 2.7477430337188053 10 3 2.505916933460938 10 4 2.9144557209374966 10 5 2.799717468852471 10 6 3.940972534559002 10 7 2.7631802078253003 10 8 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-1.7211144195616797 114 54 -1.9888773271084226 114 55 -0.5404092065623693 114 56 3.5741012974903086 115 1 1.0268294855183555 115 2 1.8511913095808765 115 3 1.3030771768686478 115 4 2.0280459846291734 115 5 2.3014078001574063 115 6 2.2195391336124217 115 7 1.7523620874737134 115 8 1.7790284847514855 115 9 2.2322526950546546 115 10 0.8000843088380165 115 11 -3.1845762991437994 115 12 -7.221007256856566 115 13 -3.064764966481995 115 14 -6.910964342457987 115 15 -1.457456095718097 115 16 -9.425067885320281 115 17 -7.902384518799181 115 18 -1.2799336458488177 115 19 -1.1296335282793066 115 20 -2.7146475822165463 115 21 2.153244375410864 115 22 1.541162968943425 115 23 0.9527415363685634 115 24 1.4744821657323541 115 25 1.328391392105722 115 26 2.332153124788732 115 27 0.5236939482746266 115 28 2.605076147890834 115 29 1.1354994767444708 115 30 2.2030157077854042 115 31 0.7659775528095238 115 32 -4.815916874406103 115 33 1.4073846289092842 115 34 1.8128166462240856 115 35 0.3082546792678045 115 36 4.879004441854015 115 37 4.46208370162346 115 38 3.3914393812578467 115 39 5.621862694275482 115 40 8.9707589009852 115 41 8.684665686367918 115 42 3.295702514210287 115 43 2.8075247104920855 115 44 -2.4566987038469037 115 45 3.466785768416795 115 46 -2.8854702099211345 115 47 -6.554511626561952 115 48 -4.155784881067184 115 49 -7.033632376096829 115 50 -2.1599351841596537 115 51 0.4792583923367495 115 52 0.14327322743383064 115 53 -0.3886021042907437 115 54 -0.17606944533502045 115 55 0.8425957195460043 115 56 3.1233623812341484 116 1 1.1572174165528395 116 2 1.6852430337188053 116 3 1.151137521696235 116 4 1.6993821915257286 116 5 1.2690802139505095 116 6 1.1107029267158666 116 7 1.3967586391978486 116 8 1.0117871054411438 116 9 0.9790199364339642 116 10 0.6028860329759453 116 11 -1.3354383681093154 116 12 -0.6498865672013956 116 13 -4.181144276826824 116 14 -9.63186951487178 116 15 -3.101852647442236 116 16 -9.655671333596143 116 17 -8.319410380868145 116 18 -2.208812956193647 116 19 -3.765409390348271 116 20 -2.943095858078614 116 21 3.3763047202384513 116 22 3.551938831012393 116 23 3.058344984644428 116 24 1.4486200967668381 116 25 2.87041725417469 116 26 3.0950841592714937 116 27 2.0549439482746266 116 28 2.689127872028763 116 29 2.884421890537574 116 30 3.642670880199198 116 31 1.1646844493612463 116 32 -6.3428565295785155 116 33 0.8696691116679034 116 34 -0.106364388258676 116 35 1.6369184723712493 116 36 1.966288924612634 116 37 2.089238874037253 116 38 3.0433790364302595 116 39 5.730698901172037 116 40 6.7821813147783025 116 41 5.534881203609299 116 42 2.2892369969689064 116 43 -8.734501151576879 116 44 -0.4793280141917329 116 45 0.25450128565817565 116 46 -4.702280554748722 116 47 -9.451063350699883 116 48 -7.309879708653391 116 49 -4.548718582993381 116 50 -3.5133834600217213 116 51 -0.9496209180080797 116 52 -4.164916427738582 116 53 -2.7970072767045373 116 54 -5.60494875567985 116 55 -2.7145163494195117 116 56 -0.49732727393826437 117 1 -0.09903258344716193 117 2 0.34774303371880677 117 3 0.4823875216962321 117 4 1.5056321915257271 117 5 0.8065802139505109 117 6 0.3419529267158694 117 7 0.2092586391978486 117 8 0.21178710544114665 117 9 0.19776993643396423 117 10 -0.0033639670240575015 117 11 -7.260438368109316 117 12 -12.212386567201396 117 13 -4.506144276826824 117 14 -5.1506195148717815 117 15 -8.364352647442235 117 16 -10.218171333596143 117 17 -8.500660380868144 117 18 -3.8713129561936483 117 19 -2.9779093903482696 117 20 -2.4180958580786154 117 21 0.9825547202384541 117 22 0.33943883101239436 117 23 1.3958449846444267 117 24 0.4486200967668381 117 25 0.9766672541746928 117 26 1.5200841592714909 117 27 1.3236939482746237 117 28 2.001627872028763 117 29 1.8406718905375712 117 30 2.892670880199198 117 31 -0.1978155506387509 117 32 -10.586606529578514 117 33 -0.3115808883320952 117 34 0.47488561174132116 117 35 -0.5693315276287514 117 36 1.6600389246126355 117 37 2.4517388740372503 117 38 3.912129036430258 117 39 4.9994489011720376 117 40 1.1759313147783033 117 41 4.153631203609297 117 42 2.826736996968908 117 43 -6.7407511515768785 117 44 2.1519219858082685 117 45 2.235751285658175 117 46 1.0852194452512798 117 47 -6.651063350699882 117 48 -1.2161297086533907 117 49 -1.31121858299338 117 50 0.3366165399782801 117 51 1.787879081991921 117 52 2.6725835722614164 117 53 0.07174272329546127 117 54 -3.4674487556798503 117 55 -0.03326634941951312 117 56 2.5026727260617356 118 1 1.2694859350713585 118 2 2.15515044112621 118 3 1.7564615957703111 118 4 2.114891450784988 118 5 2.289913547283845 118 6 2.3919529267158666 118 7 1.7370364169756272 118 8 2.5580834017374414 118 9 2.1977699364339642 118 10 2.1781175144574263 118 11 -6.867845775516724 118 12 -9.129053233868063 118 13 -9.417255387937935 118 14 -2.6746935889458534 118 15 -17.701389684479274 118 16 -6.477430592855402 118 17 -8.350660380868145 118 18 -4.410201845082536 118 19 -3.614946427385309 118 20 -3.4106884506712056 118 21 1.4955176832014132 118 22 1.470920312493874 118 23 1.0791783179777639 118 24 -0.4310095328627881 118 25 0.9248154023228352 118 26 1.136750825938158 118 27 1.5607309853116647 118 28 0.7238500942509845 118 29 1.8277089275746121 118 30 0.18896717649549544 118 31 -1.1292970321202347 118 32 -8.584754677726664 118 33 -0.6615808883320966 118 34 -0.13992920307349266 118 35 -0.7045167128139376 118 36 2.9581870727607793 118 37 4.040627762926139 118 38 4.938054962356183 118 39 7.375374827097961 118 40 8.625931314778303 118 41 7.827705277683371 118 42 1.819329589561498 118 43 2.7666562558305294 118 44 1.264884948771229 118 45 2.387603137510027 118 46 -7.820336110304277 118 47 -11.302915202551734 118 48 -9.058722301245984 118 49 -9.972329694104491 118 50 -8.855976052614315 118 51 -8.688046843934005 118 52 -8.218157168479323 118 53 -9.744923943371203 118 54 -11.121152459383554 118 55 -9.766599682752846 118 56 -7.608438385049375 119 1 1.1213377869232133 119 2 2.2662615522373244 119 3 2.0897949291036397 119 4 2.2630395989331333 119 5 2.252876510246807 119 6 2.3549158896788285 119 7 2.0333327132719248 119 8 2.446972290626327 119 9 2.3459180845821095 119 10 1.6225619589018692 119 11 -10.053030960701907 119 12 -5.980905085719915 119 13 -0.3431813138638624 119 14 -0.34136025561252126 119 15 -4.886574869664457 119 16 -4.366319481744291 119 17 -6.1654751956829585 119 18 -0.7064981413788338 119 19 -1.540872353311233 119 20 0.07079303081027533 119 21 1.6807028683865965 119 22 1.54499438656795 119 23 1.7458449846444282 119 24 -4.5791576810109405 119 25 2.0729635504709876 119 26 2.581195270382608 119 27 3.190360614941291 119 28 2.1312575016583892 119 29 2.6425237423894288 119 30 3.448226435754755 119 31 0.685517782694582 119 32 -9.436606529578516 119 33 0.7087894820382772 119 34 1.0082189450746597 119 35 0.40659439829717314 119 36 4.328557443131153 119 37 4.040627762926139 119 38 5.308425332726557 119 39 7.63463408635722 119 40 9.218523907370898 119 41 9.568446018424112 119 42 4.0415518117837195 119 43 0.544434033608308 119 44 4.672292356178637 119 45 1.1653809152878054 119 46 -3.8203361103042788 119 47 -6.3769892766258085 119 48 -7.98464822717191 119 49 -4.4908482126230105 119 50 -1.4856056822439427 119 51 -2.4287875846747475 119 52 -1.8848238351459905 119 53 -4.337516535963797 119 54 -6.491522829753924 119 55 -5.0628959790491415 119 56 -1.3121420887530775 120 1 1.2709674165528426 120 2 2.187743033718803 120 3 2.0823875216962335 120 4 2.255632191525727 120 5 2.3965802139505072 120 6 1.9519529267158688 120 7 1.5992586391978492 120 8 1.061787105441141 120 9 1.2777699364339625 120 10 0.13663603297594307 120 11 -11.940438368109316 120 12 -2.542386567201394 120 13 -10.226144276826826 120 14 -5.1606195148717795 120 15 -11.464352647442237 120 16 -9.458171333596141 120 17 -10.510660380868146 120 18 -3.481312956193648 120 19 -4.0579093903482715 120 20 -4.098095858078615 120 21 -0.42744527976154956 120 22 0.3894388310123915 120 23 -1.0941550153555752 120 24 -5.601379903233163 120 25 -0.5033327458253112 120 26 2.1500841592714934 120 27 2.843693948274627 120 28 3.3416278720287664 120 29 2.1906718905375726 120 30 1.4926708801991992 120 31 -3.6478155506387537 120 32 -11.996606529578514 120 33 -1.6215808883320975 120 34 -11.285114388258677 120 35 -2.3593315276287505 120 36 -1.5499610753873654 120 37 -0.888261125962746 120 38 0.09212903643025783 120 39 -0.43055109882796216 120 40 -2.934068685221696 120 41 -0.7663687963907009 120 42 -4.693263003031095 120 43 -5.400751151576879 120 44 -5.698078014191733 120 45 -7.184248714341825 120 46 -10.984780554748722 120 47 -10.731063350699882 120 48 -9.58612970865339 120 49 -13.661218582993381 120 50 -7.023383460021721 120 51 -8.28212091800808 120 52 -8.117416427738583 120 53 -10.078257276704537 120 54 -13.69744875567985 120 55 -11.913266349419512 120 56 -7.257327273938264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1040.0 5 13.0 6 14.0 7 57.0 8 109.0 9 71.0 10 67.0 11 46.0 12 77.0 13 73.0 14 115.0 15 144.0 16 214.0 17 255.0 18 313.0 19 396.0 20 564.0 21 636.0 22 762.0 23 910.0 24 957.0 25 1153.0 26 1194.0 27 1234.0 28 1430.0 29 1428.0 30 1670.0 31 1885.0 32 2321.0 33 2758.0 34 3349.0 35 3714.0 36 4171.0 37 3554.0 38 1870.0 39 451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 98.16993464052287 1.7531718569780852 0.05895168524926311 0.017941817249775727 8 98.2237600922722 0.44598231449442516 1.2789952582340125 0.05126233499935922 9 97.80039480093316 1.3946214781962212 0.44351013920578364 0.36147358166482935 10 55.1762142765603 28.791490452390107 6.620530565167243 9.411764705882353 11 34.13133746860101 28.112985082278158 17.337366073717128 20.4183113754037 12 33.2077406125849 14.827630398564654 22.555427399718056 29.409201589132383 13 24.992951428937587 13.69985902857875 27.210047417659872 34.09714212482379 14 22.240455372939156 15.527806979308224 27.101869182841465 35.129868464911155 15 23.31518389151428 15.36624203821656 28.647010478734337 32.67156359153483 16 27.035204225532677 17.97902617881593 25.70190507935694 29.283864516294457 17 28.336324019482184 19.625736990515254 22.89156626506024 29.14637272494232 18 29.121808674254073 22.618681431354453 23.346662565364504 24.912847329026967 19 29.384165726592144 23.47964311352682 19.7954055994257 27.340785560455338 20 27.15036533777721 24.617356749134725 22.235610819125753 25.996667093962312 21 27.86208664445014 20.05383235067931 25.16277877467316 26.921302230197387 22 30.14481609637319 20.30244777649622 24.862232474689222 24.690503652441368 23 30.185825964372675 22.896321927463795 23.957452261950532 22.960399846212994 24 28.13020633089837 22.98859413046264 22.76560297321543 26.115596565423555 25 26.523132128668458 23.665256952454182 24.190695886197616 25.620915032679736 26 26.7649400344128 20.406276483730963 27.258018952720924 25.570764529135314 27 25.82829008103395 18.186480664683558 28.22340752897733 27.761821725305158 28 27.734134319162045 19.801293900184845 27.952351612240705 24.512220168412405 29 24.816694867456288 20.72501666410296 29.938983746090347 24.51930472235041 30 22.872013126858786 20.579940518921134 32.84278535534817 23.70526099887191 31 23.799126637554586 21.230413562805033 32.11148214744413 22.85897765219625 32 25.746000820344545 18.12192370795734 31.50379409351928 24.628281378178833 33 25.837271419765766 19.524347647421408 34.08670638997329 20.55167454283953 34 23.487453173910815 19.754195104428593 36.660337661004775 20.09801406065582 35 22.15809992818303 22.899353647276087 36.72412024212578 18.2184261824151 36 22.953681832186504 21.313032761630893 35.226455787203456 20.506829618979154 37 23.155405926344734 22.595153041556372 34.09832695124772 20.151114080851173 38 24.944795357674728 22.808504082575876 34.16782211266882 18.078878447080573 39 22.336867023466446 18.029538904899137 31.993618773157678 27.639975298476738 40 15.043220229647789 15.04063991743001 26.02760934073023 43.88853051219198 41 16.083626578348166 16.898675222521216 27.944524943075965 39.07317325605465 42 21.354982914986 21.198263237674382 36.68011201603165 20.766641831307968 43 13.047057915455271 12.892685311446728 28.103532559755063 45.95672421334294 44 15.377480030919866 14.730739500128834 26.3102293223396 43.581551146611694 45 22.251879988707234 20.23714806354747 36.645022200549235 20.86594974719606 46 21.356010466369092 20.89425888871787 36.67077112513468 21.07895951977836 47 19.367659879993845 19.30098979434843 34.02225755166931 27.309092773988407 48 20.14209500359085 22.84036113676003 37.72186313737561 19.29568072227352 49 14.407368339808075 15.351565514934782 26.17767372456199 44.06339242069516 50 17.858519413109953 23.3651103630804 37.998817997276255 20.77755222653339 51 20.42186495176849 24.115755627009648 36.22893890675241 19.233440514469454 52 21.739577972207925 24.61399897066392 34.727225939269175 18.919197117858978 53 20.770613922424864 23.637297713845363 34.87541741587465 20.716670947855125 54 18.014526602161023 21.001976233862894 32.32553961450607 28.65795754947001 55 22.589177944990883 21.97026117773954 36.16682503402758 19.273735843241994 56 22.121733768402663 22.710105084658668 34.34649674982657 20.821664397112098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 16.5 13 42.0 14 53.0 15 80.5 16 108.0 17 133.0 18 158.0 19 200.0 20 242.0 21 259.5 22 327.5 23 378.0 24 473.0 25 568.0 26 684.0 27 800.0 28 922.0 29 1179.5 30 1315.0 31 1334.0 32 1353.0 33 1314.5 34 1276.0 35 1159.5 36 1043.0 37 968.5 38 787.5 39 681.0 40 627.5 41 574.0 42 575.0 43 576.0 44 604.0 45 632.0 46 660.5 47 757.0 48 825.0 49 876.0 50 927.0 51 999.0 52 1071.0 53 1191.5 54 1505.0 55 1698.0 56 1802.0 57 1906.0 58 2048.5 59 2191.0 60 2415.5 61 2640.0 62 2682.0 63 2548.5 64 2373.0 65 2301.0 66 2229.0 67 2006.5 68 1784.0 69 1667.0 70 1550.0 71 1363.5 72 981.0 73 785.0 74 640.5 75 496.0 76 419.0 77 342.0 78 251.5 79 119.0 80 77.0 81 55.5 82 34.0 83 22.0 84 10.0 85 8.5 86 7.0 87 3.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02050493399974369 10 0.0 11 0.0025631167499679613 12 0.0 13 0.0 14 0.03588363449955145 15 0.20248622324746893 16 0.03588363449955145 17 0.012815583749839805 18 0.007689350249903883 19 0.028194284249647573 20 0.02563116749967961 21 0.012815583749839805 22 0.0 23 0.0 24 0.0 25 0.0 26 0.19479687299756504 27 0.04869921824939126 28 0.16147635524798157 29 0.02306805074971165 30 0.028194284249647573 31 0.2178649237472767 32 0.017941817249775727 33 0.20248622324746893 34 0.10508778674868641 35 0.06920415224913494 36 0.17172882224785338 37 0.26656414199666795 38 0.1768550557477893 39 0.3870306292451621 40 0.6664103549916698 41 0.9406638472382416 42 0.23580674099705243 43 0.37934127899525827 44 0.525438933743432 45 0.13328207099833397 46 0.08458285274894271 47 0.04357298474945533 48 0.06920415224913494 49 0.3742150454953223 50 0.2511854414968602 51 0.35883634499551453 52 0.397283096245034 53 0.2178649237472767 54 0.13328207099833397 55 0.19479687299756504 56 0.24093297449698836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 39015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.2323465333846 #Duplication Level Percentage of deduplicated Percentage of total 1 93.96219911529843 83.84467512495193 2 4.087436088929741 7.294630270408817 3 0.9938530476245189 2.6605151864667436 4 0.36479577181593614 1.3020633089837241 5 0.1551100132130752 0.6920415224913495 6 0.1148963060837594 0.6151480199923107 7 0.07181019130234963 0.44854543124439317 8 0.03159648417303384 0.2255542739971806 9 0.03446889182512782 0.27681660899653976 >10 0.17808927442982708 2.355504293220556 >50 0.00574481530418797 0.2845059592464437 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 58 0.14866077149814175 No Hit AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 53 0.13584518774830193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002563116749967961 0.0 0.0 0.0 0.0 11 0.002563116749967961 0.0 0.0 0.0 0.0 12 0.002563116749967961 0.0 0.0 0.0 0.0 13 0.002563116749967961 0.0 0.0 0.0 0.0 14 0.010252466999871843 0.0 0.0 0.0 0.0 15 0.010252466999871843 0.0 0.0 0.0 0.0 16 0.010252466999871843 0.0 0.0 0.0 0.0 17 0.010252466999871843 0.0 0.0 0.0 0.0 18 0.012815583749839805 0.0 0.0 0.0 0.0 19 0.017941817249775727 0.0 0.0 0.0 0.0 20 0.020504933999743687 0.0 0.0 0.0 0.0 21 0.02563116749967961 0.0 0.0 0.0 0.0 22 0.02819428424964757 0.0 0.0 0.0 0.0 23 0.03588363449955145 0.0 0.0 0.0 0.0 24 0.03844675124951941 0.0 0.0 0.0 0.0 25 0.04100986799948737 0.0 0.0 0.0 0.0 26 0.0461361014994233 0.0 0.0 0.0 0.0 27 0.05126233499935922 0.0 0.0 0.0 0.0 28 0.05126233499935922 0.0 0.0 0.0 0.0 29 0.05382545174932718 0.0 0.0 0.0 0.0 30 0.05638856849929514 0.0 0.0 0.0 0.0 31 0.05638856849929514 0.0 0.0 0.0 0.0 32 0.05638856849929514 0.0 0.0 0.0 0.0 33 0.05638856849929514 0.0 0.0 0.0 0.0 34 0.05638856849929514 0.0 0.0 0.0 0.0 35 0.05638856849929514 0.0 0.0 0.0 0.0 36 0.05638856849929514 0.0 0.0 0.0 0.0 37 0.05895168524926311 0.0 0.0 0.0 0.0 38 0.05895168524926311 0.0 0.0 0.0 0.0 39 0.05895168524926311 0.0 0.0 0.0 0.0 40 0.05895168524926311 0.0 0.0 0.0 0.0 41 0.06151480199923107 0.0 0.0 0.0 0.0 42 0.06151480199923107 0.0 0.0 0.0 0.0 43 0.06151480199923107 0.0 0.0 0.0 0.0 44 0.06407791874919902 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACCAA 15 0.0080603035 50.478542 40 ACCAACC 20 3.943479E-4 50.478542 38 AACAAAC 15 0.008143644 50.3476 37 ACCAGCC 15 0.008185553 50.28238 42 TGCAAGA 15 0.008227623 50.217335 47 CCTGCAC 15 0.008227623 50.217335 36 CCGTCTC 15 0.008227623 50.217335 43 CAAGGAG 15 0.008227623 50.217335 49 CCAAGGA 15 0.008227623 50.217335 48 TGGCTCT 15 0.008227623 50.217335 46 GTGCGTA 15 0.008269854 50.152454 44 CGACCGT 15 0.008269854 50.152454 44 CGACTCC 15 0.008269854 50.152454 44 CGTCTCC 15 0.008269854 50.152454 44 TCTGTGA 20 4.0980466E-4 50.08774 45 ATCTCGT 15 0.008312246 50.087738 33 TGCGTAC 15 0.008312246 50.087738 45 ACCCGTC 15 0.008312246 50.087738 50 GCTGCAA 15 0.008312246 50.087738 45 GACTCCT 15 0.008312246 50.087738 45 >>END_MODULE