FastQCFastQC Report
Fri 17 Jun 2016
SRR1526282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58400
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA1570.2688356164383562No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1300.2226027397260274No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1250.21404109589041095No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1030.17636986301369864No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAACCAAAAAAAAAAAAAAA980.1678082191780822No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAACCAAAACAAAAAAA840.14383561643835616No Hit
AGGGTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT820.14041095890410957No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC740.1267123287671233No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAACCAAAAAAAAAAAAAAA740.1267123287671233No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACAAAAAAAAAAAAAAA640.1095890410958904No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC610.10445205479452055No Hit
AGGGTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT590.10102739726027397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACC251.9608358E-550.42968838
CCAGCGT150.00811161950.42968839
CAACCAA309.714258E-750.42968840
TCTGGCC150.00811161950.42968838
TTCCCGG150.00811161950.42968841
ATTCCCG150.00811161950.42968840
CATATTG150.00811161950.42968840
AAACCAC150.00819554450.29870237
TACAAAC204.030507E-450.298737
AAGGCAT150.008251850550.21175435
CCGCTCT301.0069507E-650.16839248
AAAGGCA150.00828011250.16839234
CTCGCGC150.00828011250.16839249
ACCGATC204.099968E-450.12510750
TAGCAAG150.00830844550.12510747
ACGGGCA204.099968E-450.12510744
TACGAAG150.00830844550.12510747
CTACCAA150.00830844550.12510746
CAAAGGC204.117481E-450.08189833
CCTAGCA150.0083368550.08189845