##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526282_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58400 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.00530821917808 38.0 37.0 39.0 33.0 40.0 2 36.25566780821918 38.0 36.0 39.0 31.0 40.0 3 35.98640410958904 38.0 36.0 39.0 30.0 40.0 4 36.053852739726025 38.0 36.0 39.0 30.0 40.0 5 34.80739726027397 38.0 35.0 39.0 26.0 40.0 6 36.39042808219178 38.0 37.0 39.0 32.0 40.0 7 36.02297945205479 38.0 36.0 39.0 31.0 40.0 8 35.87025684931507 38.0 35.0 39.0 30.0 40.0 9 35.94364726027397 38.0 36.0 39.0 30.0 40.0 10 35.92184931506849 38.0 36.0 39.0 30.0 40.0 11 35.20434931506849 38.0 35.0 39.0 29.0 40.0 12 32.98616438356164 38.0 31.0 39.0 18.0 40.0 13 32.39792808219178 37.0 30.0 39.0 16.0 40.0 14 32.22931506849315 37.0 29.0 39.0 14.0 40.0 15 32.30407534246575 36.0 29.0 39.0 16.0 40.0 16 32.394657534246576 36.0 30.0 39.0 16.0 40.0 17 32.467448630136985 36.0 30.0 39.0 17.0 40.0 18 32.62916095890411 36.0 30.0 39.0 19.0 40.0 19 33.47642123287671 36.0 31.0 39.0 24.0 40.0 20 33.57443493150685 37.0 31.0 39.0 24.0 40.0 21 34.637191780821915 38.0 34.0 39.0 26.0 40.0 22 34.35169520547945 38.0 33.0 39.0 24.0 40.0 23 34.6121404109589 38.0 34.0 39.0 26.0 40.0 24 34.22537671232877 38.0 33.0 39.0 24.0 40.0 25 34.519366438356165 38.0 33.0 39.0 25.0 40.0 26 34.735342465753426 38.0 35.0 39.0 27.0 40.0 27 34.62748287671233 38.0 35.0 39.0 26.0 40.0 28 34.67751712328767 38.0 35.0 39.0 26.0 40.0 29 34.59777397260274 38.0 35.0 39.0 26.0 40.0 30 34.392859589041095 38.0 34.0 39.0 25.0 40.0 31 34.16296232876712 38.0 34.0 39.0 24.0 40.0 32 33.01013698630137 38.0 32.0 39.0 17.0 40.0 33 34.325873287671236 38.0 34.0 39.0 25.0 40.0 34 34.00888698630137 38.0 34.0 39.0 23.0 40.0 35 34.08849315068493 38.0 34.0 39.0 24.0 40.0 36 32.01655821917808 35.0 31.0 38.0 18.0 39.0 37 31.70345890410959 35.0 31.0 38.0 16.0 39.0 38 31.020513698630136 35.0 30.0 38.0 10.0 39.0 39 29.033972602739727 33.0 26.0 38.0 4.0 39.0 40 24.955633561643836 31.0 11.0 37.0 4.0 39.0 41 21.735650684931507 25.0 9.0 33.0 4.0 37.0 42 25.544554794520547 30.0 20.0 34.0 4.0 37.0 43 21.746917808219177 26.0 7.0 33.0 4.0 37.0 44 22.725291095890412 28.0 9.0 33.0 4.0 37.0 45 25.21015410958904 30.0 19.0 34.0 4.0 38.0 46 25.248801369863013 30.0 14.0 36.0 4.0 38.0 47 24.462054794520547 30.0 8.0 36.0 4.0 38.0 48 24.46777397260274 30.0 10.0 35.0 4.0 38.0 49 21.684931506849313 26.0 4.0 35.0 4.0 38.0 50 24.249349315068493 30.0 4.0 35.0 4.0 38.0 51 25.747431506849313 32.0 4.0 38.0 4.0 39.0 52 25.549743150684932 32.0 4.0 38.0 4.0 39.0 53 25.15220890410959 32.0 4.0 38.0 4.0 39.0 54 24.230736301369863 31.0 4.0 37.0 4.0 39.0 55 24.479623287671235 31.0 4.0 37.0 4.0 39.0 56 23.799143835616437 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8958055757516235 1 2 2.2621328620979355 1 3 2.6509608570841934 1 4 2.424002638065268 1 5 2.3050956244661265 1 6 2.1518206595972416 1 7 2.5697249964744913 1 8 2.8162119095173566 1 9 2.6098764166092536 1 10 2.2941245121889793 1 11 -3.009419840483858 1 12 -6.980314051181203 1 13 -21.558970007721502 1 14 -18.24416253425126 1 15 -10.699185160867799 1 16 -21.297186791098856 1 17 -21.278835411684717 1 18 -19.793808140092 1 19 -5.1496894865596765 1 20 -9.527946555222155 1 21 -7.988635215168028 1 22 -3.3423760499156785 1 23 -3.020390669391915 1 24 -3.784698507486752 1 25 -3.6446484697765307 1 26 2.600523728633405 1 27 2.2485399840903924 1 28 3.0728572146562954 1 29 2.323649983330526 1 30 1.955233743783161 1 31 0.8349433756302389 1 32 -12.319261942303044 1 33 0.6675272916645767 1 34 1.025247542439537 1 35 0.9352115017028027 1 36 4.390689969355126 1 37 4.6143755496073275 1 38 5.738586494114461 1 39 7.678997999714056 1 40 10.968292646053573 1 41 -3.066275214796576 1 42 -7.528798329992128 1 43 -13.982975982629931 1 44 -15.779194270533647 1 45 -14.533628863847166 1 46 -17.68045558360849 1 47 -17.71366182593396 1 48 -15.716799772600247 1 49 -13.918253343861783 1 50 -16.305576125230942 1 51 -16.583891587914735 1 52 -16.5170747162457 1 53 -17.079583488141566 1 54 -17.865549768977615 1 55 -16.60944730379076 1 56 -15.297487095065998 2 1 1.470094300725279 2 2 2.2237766977143707 2 3 2.5852074324266567 2 4 2.4060890448092067 2 5 2.5364970997032117 2 6 2.21441286191547 2 7 2.5825806339876607 2 8 2.854778822056872 2 9 2.8229428022783836 2 10 2.4880128156663233 2 11 -2.5881342767325393 2 12 -9.692221321992584 2 13 -10.558759259565548 2 14 -14.190211006327129 2 15 -7.807509713027969 2 16 -2.3996103948923206 2 17 -1.4697732409787108 2 18 -9.367043124285889 2 19 -1.6618075055270118 2 20 -2.865986597371787 2 21 2.7999843844104717 2 22 3.0340201566175153 2 23 2.5815060640116627 2 24 3.294542799151813 2 25 2.6307993700548735 2 26 3.534559555819918 2 27 3.429994146366475 2 28 3.0897170671325824 2 29 3.450309625058658 2 30 3.7779945446261536 2 31 -1.1840239584055858 2 32 -10.215573849573858 2 33 -1.2125570075978018 2 34 -0.856733490226425 2 35 -1.484177328644936 2 36 4.564135701704966 2 37 4.4074208604608565 2 38 5.0338446606055065 2 39 6.923887356932177 2 40 9.64142225616948 2 41 7.656801708280348 2 42 2.874152144191225 2 43 -0.7130075948533268 2 44 -9.385305967056302 2 45 1.7504596503783354 2 46 -9.950213223229147 2 47 -10.345906293794869 2 48 -5.573491026551775 2 49 -10.184639012987178 2 50 -5.828020803840005 2 51 -3.6593394277461364 2 52 -4.265862914348965 2 53 -5.4650418653807655 2 54 -13.800007092476035 2 55 -5.236633815908778 2 56 -3.976306905392658 3 1 1.4059270332414968 3 2 2.704238125255827 3 3 3.093066120242085 3 4 2.727646362761625 3 5 3.0929498754782685 3 6 2.8085008215405622 3 7 3.2620326887821847 3 8 3.2510297232825422 3 9 3.3685808700505504 3 10 3.1265131761565854 3 11 1.928231981378488 3 12 3.0051110500333706 3 13 -4.545204825535265 3 14 -3.551854841943566 3 15 -1.742909857224074 3 16 3.6655662453383933 3 17 3.3867516328496947 3 18 2.9333173659808764 3 19 2.0114441166791863 3 20 1.9400696391098293 3 21 3.7943607362489757 3 22 4.43171301891023 3 23 3.640338075547355 3 24 4.391009994537541 3 25 3.4727604371060607 3 26 2.660442756973481 3 27 3.2477302674911996 3 28 2.7951244211340267 3 29 3.238629740415547 3 30 1.8945049988438925 3 31 2.9013401367638423 3 32 2.1584708512192243 3 33 2.205179113526924 3 34 3.3863811456784063 3 35 2.689867372148143 3 36 5.7510138559948025 3 37 5.496966642724733 3 38 6.118343579134706 3 39 8.92677127906628 3 40 11.523758233098103 3 41 10.646275392490875 3 42 8.411282641667793 3 43 3.5975908189895023 3 44 4.7584575513287 3 45 8.053415670565787 3 46 0.005374375905677908 3 47 -3.8723662793752567 3 48 0.6329977982499528 3 49 -9.370075206209961 3 50 -0.11367329122284531 3 51 -0.6907741790078532 3 52 -0.6247670239380057 3 53 -0.51925960150189 3 54 -5.142472845900691 3 55 -0.4483137005518927 3 56 1.5859137146506015 4 1 1.60119019113624 4 2 2.832132862097936 4 3 2.550960857084192 4 4 2.2955410996037244 4 5 2.886634086004584 4 6 2.907974505751092 4 7 2.852032688782181 4 8 3.1531349864404348 4 9 3.0091071858400227 4 10 3.433355281419743 4 11 3.319810928746911 4 12 2.7735321026649515 4 13 5.557953069201577 4 14 2.8881451580564352 4 15 6.71235330067066 4 16 6.888197824285758 4 17 5.858856896007595 4 18 5.245422629138773 4 19 4.907233590363397 4 20 4.837438060162462 4 21 3.557518630985818 4 22 4.012239334699707 4 23 3.361917022915776 4 24 3.398378415590173 4 25 3.653813068685004 4 26 3.3836006517103243 4 27 3.069309214859622 4 28 3.1567033685024484 4 29 2.94441921409976 4 30 3.2029260514754725 4 31 3.2818664525533165 4 32 3.7668919038508015 4 33 2.896758060895344 4 34 3.6021706193626173 4 35 2.1382884247797236 4 36 4.588382277047433 4 37 4.555914011145784 4 38 5.847817263345231 4 39 7.323613384329434 4 40 10.020600338361259 4 41 5.286801708280347 4 42 7.858124746930951 4 43 6.6354855558316075 4 44 11.250036498697124 4 45 8.878678828460526 4 46 7.568005954853046 4 47 7.63710740483527 4 48 6.84089253509206 4 49 5.410977425368987 4 50 7.302116182461365 4 51 5.637646873623726 4 52 5.275232976061993 4 53 5.08810881955074 4 54 0.8675271540993101 4 55 5.867475773132316 4 56 5.233282135703234 5 1 1.7134373821474753 5 2 3.1677508396260237 5 3 2.893657486297677 5 4 2.908013009716086 5 5 3.953150939937167 5 6 3.2721318091218734 5 7 2.9833810033889208 5 8 3.0122361100359427 5 9 2.763376848761375 5 10 2.5689732589478353 5 11 4.1171142995334264 5 12 6.560048956597534 5 13 6.655031720886971 5 14 5.9335384164833975 5 15 7.236510604041445 5 16 7.136175352375648 5 17 6.914250154434555 5 18 6.222613640374725 5 19 5.477795388116206 5 20 5.370584127578191 5 21 3.457518630985817 5 22 4.325385402115437 5 23 3.8427035397697082 5 24 4.6462435841295004 5 25 3.785947900145679 5 26 3.5280950337327965 5 27 2.672567641825914 5 28 3.091871907828292 5 29 3.1072282028638014 5 30 2.822476613273224 5 31 3.5410799356993863 5 32 5.037004263401361 5 33 3.49069064516501 5 34 4.281945900261491 5 35 3.835816514667364 5 36 5.10961823210361 5 37 5.655689292044659 5 38 6.083098162221635 5 39 8.225411137138423 5 40 6.186443034990475 5 41 0.7466893487297845 5 42 8.95711351097589 5 43 12.748743982797897 5 44 11.482171330157794 5 45 9.086768716100973 5 46 10.936657640246306 5 47 11.550815270003806 5 48 11.635836355316776 5 49 12.246483043346515 5 50 10.74593640718047 5 51 10.500118783736085 5 52 9.853884661455254 5 53 11.016872864494562 5 54 11.059774345110547 5 55 10.880958919199735 5 56 10.380473146939188 6 1 1.640601955842122 6 2 2.4386034503332255 6 3 2.756843210025366 6 4 2.4620116878390235 6 5 2.75486938012223 6 6 2.927386270456971 6 7 2.6149738652527716 6 8 2.9519585158521977 6 9 2.6849895387812026 6 10 2.5221788108315124 6 11 3.500987399335145 6 12 6.644120337959066 6 13 7.082658951554517 6 14 6.671674569821139 6 15 7.2682356536118355 6 16 6.941727236050465 6 17 6.499445131301712 6 18 5.6224814526681826 6 19 5.186057119775164 6 20 4.875673354280103 6 21 3.222224513338759 6 22 4.312827569993821 6 23 3.34250525820989 6 24 4.067790180296058 6 25 3.8820483628026565 6 26 2.994188887004441 6 27 3.3263680383890346 6 28 2.8149386626200936 6 29 3.366183919982113 6 30 3.6746907573578227 6 31 2.7824546878474337 6 32 4.091597786203739 6 33 3.542640413836523 6 34 3.47864120759791 6 35 3.0700531306620746 6 36 3.8513234535180167 6 37 3.064149305263431 6 38 4.319581969227588 6 39 5.922436913741201 6 40 3.8723650442436153 6 41 2.0332722965156407 6 42 7.712830629283893 6 43 11.743720849949252 6 44 10.072389439873593 6 45 7.641031769636996 6 46 10.352123601911867 6 47 10.893577993070561 6 48 10.461480770386174 6 49 12.533330366545457 6 50 10.590351476579013 6 51 9.12470569715314 6 52 8.857585917238467 6 53 9.545755878374269 6 54 9.598703624687545 6 55 9.23218165548526 6 56 8.827399782762058 7 1 1.6897616197076673 7 2 2.8764185763836494 7 3 3.312865618988951 7 4 3.1593506234132533 7 5 3.5356817050522054 7 6 3.324164981941564 7 7 3.1572707840202767 7 8 3.7931349864404353 7 9 3.548154804887645 7 10 3.2062124242768917 7 11 4.376477595413576 7 12 6.511627340760192 7 13 7.41319116443967 7 14 7.879097539008814 7 15 7.645686634003997 7 16 7.744388300476235 7 17 7.109809276959972 7 18 6.642089295805434 7 19 5.61770978083959 7 20 6.018390441114839 7 21 4.338471011938196 7 22 4.663667906128275 7 23 4.06715511815387 7 24 3.5931403203520773 7 25 4.327146402018343 7 26 3.016457794567472 7 27 3.8507377862881924 7 28 3.9171795589786385 7 29 3.5448954045759464 7 30 3.736735575284996 7 31 3.9832950239818885 7 32 4.38879666575556 7 33 3.9581866323239154 7 34 4.328361095553092 7 35 3.5725741390654377 7 36 3.5150489437140955 7 37 3.3925806778124503 7 38 4.307817263345232 7 39 6.104089574805627 7 40 -0.032256804495879976 7 41 -9.08129352981489 7 42 7.064791413597618 7 43 11.236437936783986 7 44 9.130512689173312 7 45 6.225345495127193 7 46 8.905625002472096 7 47 9.56472645245432 7 48 9.007559201758728 7 49 11.6152631396547 7 50 8.944973325318507 7 51 10.680027826004675 7 52 10.751423452252467 7 53 10.861442152884077 7 54 11.40514620171836 7 55 11.119856725513266 7 56 9.764234516655613 8 1 1.4148046489675608 8 2 2.5175545488449202 8 3 3.231683748650454 8 4 2.825300135748307 8 5 2.612417218534702 8 6 3.224480529847476 8 7 3.3993820863725404 8 8 3.2810867936693526 8 9 3.516456583430383 8 10 2.2922709440703457 8 11 4.248967555252932 8 12 6.556664632785434 8 13 7.197471141490734 8 14 7.091518652032338 8 15 6.815365348863434 8 16 6.9639809568158775 8 17 6.795121956248558 8 18 5.842892508656838 8 19 5.216390216869421 8 20 4.983823602331135 8 21 3.2406511611062996 8 22 4.288022467229823 8 23 3.7851700349639685 8 24 3.817896487879331 8 25 2.999837165070552 8 26 2.1476970372524917 8 27 2.556417648594561 8 28 2.250799754044614 8 29 2.9447806598828876 8 30 1.9689501478610154 8 31 2.50222789833645 8 32 3.861831662886946 8 33 2.1186857717387184 8 34 2.306989896471052 8 35 3.231782400683336 8 36 1.0863340842763485 8 37 0.8132634087361481 8 38 2.0909497934657146 8 39 3.0491555530041374 8 40 -0.8863876134459652 8 41 -9.897415159189531 8 42 3.7746307710273364 8 43 8.210907242578596 8 44 7.286422040865798 8 45 4.522534250147274 8 46 7.821861376539793 8 47 7.625541139775027 8 48 8.277639523043867 8 49 11.060615979585855 8 50 8.199706543907148 8 51 8.104152897720112 8 52 8.341498036302958 8 53 9.473891952080859 8 54 10.014153660123405 8 55 9.75181312252991 8 56 9.036896593534557 9 1 1.6460216518103934 9 2 2.6171890418732175 9 3 3.1857923177583487 9 4 2.9979006501655263 9 5 3.1441621758922267 9 6 3.0474127079982836 9 7 2.9946169584451 9 8 3.0122361100359427 9 9 3.1903431408962035 9 10 2.479085618498395 9 11 3.836215423128934 9 12 6.8409478330020335 9 13 6.385368799538657 9 14 6.731291225472166 9 15 7.652240941120102 9 16 7.70920906024082 9 17 5.453575997131189 9 18 6.503512516779217 9 19 5.410379657779131 9 20 5.145865026454594 9 21 4.333923125367839 9 22 4.662464053800832 9 23 3.887647359994425 9 24 4.7361312245789335 9 25 3.684824304640067 9 26 4.247196157328304 9 27 3.268073259803444 9 28 3.6873775258058217 9 29 3.601610225335712 9 30 3.406746276194575 9 31 3.6309675761488265 9 32 4.677453701603607 9 33 3.9738367125807414 9 34 4.326889720486214 9 35 4.026827750622417 9 36 3.1433260972721477 9 37 3.4309701909210695 9 38 3.7460195105362466 9 39 5.113051586576631 9 40 0.7145329226309265 9 41 -2.4218499771129096 9 42 6.979585421088252 9 43 11.17571027493273 9 44 10.673182566112853 9 45 7.839577704865018 9 46 9.352387977324955 9 47 10.618231000340888 9 48 9.871791411496552 9 49 13.426258324245389 9 50 9.59986899145013 9 51 9.230455862387771 9 52 9.50557005471368 9 53 10.0505807296631 9 54 10.093482210279085 9 55 9.453992627064903 9 56 8.975978764916714 10 1 1.3770438496728232 10 2 2.162132862097934 10 3 2.7338876863524817 10 4 2.287980123993975 10 5 2.642243842102147 10 6 3.0735842618486515 10 7 3.257154640001694 10 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1.646560330104343 115 32 -3.9547407492104227 115 33 1.0245131629361595 115 34 1.530742047934048 115 35 0.8174720982491124 115 36 3.0371577872515125 115 37 2.4555058478804774 115 38 2.8588376715084998 115 39 5.58198073126821 115 40 6.962641154687795 115 41 8.75884252460688 115 42 2.4950635224411535 115 43 3.008546780321403 115 44 0.2716691517583456 115 45 2.9872502570319526 115 46 -2.0195450655551177 115 47 -6.237994635981057 115 48 -5.5111482812344725 115 49 -7.430655227692238 115 50 -0.09584300121210276 115 51 1.6613203430114822 115 52 1.4282942005517896 115 53 0.43627208485686353 115 54 1.4493638887931866 115 55 0.42257781394864224 115 56 2.4802209112134364 116 1 1.244523524469571 116 2 1.862132862097937 116 3 2.6509608570841934 116 4 2.6633188773815064 116 5 2.8333007526712493 116 6 1.9757522835288697 116 7 1.9953660221155118 116 8 2.1598016531071025 116 9 2.6902182969511372 116 10 2.1522441703086344 116 11 -0.06241129347531427 116 12 -1.13757900844616 116 13 -5.748713597465091 116 14 -8.174077064165786 116 15 -3.6376466993293377 116 16 -10.037357731269797 116 17 -8.837809770659074 116 18 -2.8912440375278976 116 19 -3.8505441874143784 116 20 -3.023673050948652 116 21 2.257518630985814 116 22 2.8866837791441498 116 23 1.938583689582444 116 24 1.5606006378123922 116 25 2.69381306868501 116 26 2.701378429488102 116 27 2.2570869926373973 116 28 2.2933700351691186 116 29 2.198863658544198 116 30 2.6407038292532476 116 31 1.3118664525533177 116 32 -4.695330318371422 116 33 1.8589802831175675 116 34 1.124392841584843 116 35 0.947177313668611 116 36 2.5650489437140997 116 37 3.342580677812453 116 38 4.46337281890079 116 39 5.819168939884996 116 40 8.371711449472375 116 41 8.212357263835905 116 42 4.370346969153172 116 43 -8.31118111083506 116 44 -0.29885239019176524 116 45 4.0920121617938605 116 46 -3.319771822924732 116 47 -7.371781484053621 116 48 -7.586885242685717 116 49 -5.749022574631015 116 50 -1.3978838175386343 116 51 2.0109802069570613 116 52 2.108566309395325 116 53 2.3392199306618515 116 54 -2.386917290345135 116 55 0.9785868842434269 116 56 2.0221710245921223 117 1 0.3111901911362338 117 2 2.0287995287646012 117 3 2.3176275237508577 117 4 2.0855410996037307 117 5 2.799967419337918 117 6 1.5313078390844197 117 7 1.428699355448849 117 8 1.8931349864404368 117 9 1.51244051917336 117 10 1.1966886147530786 117 11 -10.34018907125309 117 12 -10.82646789733505 117 13 -10.182046930798425 117 14 -8.451854841943565 117 15 -8.804313365996006 117 16 -13.681802175714239 117 17 -9.87114310399241 117 18 -3.5245773708612305 117 19 -3.306099742969934 117 20 -2.6125619398375406 117 21 0.9575186309858168 117 22 1.0755726680330397 117 23 1.1052503562491083 117 24 -2.9616215844098264 117 25 1.0604797353516773 117 26 2.6569339850436577 117 27 3.14597588152629 117 28 3.0600367018357844 117 29 3.0544192140997595 117 30 2.429592718142139 117 31 0.8118664525533177 117 32 -10.106441429482533 117 33 1.8034247275620103 117 34 2.4688372860292844 117 35 1.5916217581130567 117 36 3.598382277047431 117 37 4.64258067781245 117 38 4.2744839300119 117 39 6.396946717662772 117 40 6.493933671694595 117 41 6.023468374947015 117 42 2.8481247469309494 117 43 -4.911181110835061 117 44 -0.009963501302877376 117 45 3.125345495127192 117 46 -1.2419940451469564 117 47 -6.216225928498066 117 48 -5.242440798241272 117 49 -5.582355907964347 117 50 0.7354495157946985 117 51 2.610980206957059 117 52 1.9752329760619922 117 53 0.6947754862174094 117 54 -3.1091395125673564 117 55 -0.999190893534351 117 56 2.4332821357032337 118 1 1.240356857802901 118 2 2.3287995287645984 118 3 2.050960857084192 118 4 2.489707766270392 118 5 3.2083007526712493 118 6 1.7438078390844254 118 7 1.9370326887821818 118 8 2.3556349864404353 118 9 2.3666071858400244 118 10 1.6883552814197458 118 11 -6.769355737919756 118 12 -8.330634564001716 118 13 -4.682046930798425 118 14 -0.43935484194356533 118 15 -15.083480032662672 118 16 -5.502635509047572 118 17 -10.125309770659076 118 18 -2.7954107041945626 118 19 -2.6352664096366 118 20 -2.4667286065042084 118 21 0.4783519643191525 118 22 -0.34526066530029453 118 23 0.7010836895824397 118 24 -5.915788251076496 118 25 0.2854797353516716 118 26 0.1652673183769906 118 27 0.6959758815262873 118 28 0.768370035169113 118 29 0.6460858807664209 118 30 1.3379260514754705 118 31 0.6826997858866548 118 32 -7.914774762815867 118 33 0.42842472756201033 118 34 -0.022829380637382712 118 35 0.004121758113058149 118 36 1.9733822770474312 118 37 2.538414011145786 118 38 3.0161505966785676 118 39 5.705280050996102 118 40 8.839767005027927 118 41 7.86513504161368 118 42 2.252291413597618 118 43 3.5638188891649385 118 44 1.9817031653637898 118 45 5.267012161793858 118 46 -1.829494045146955 118 47 -3.7328925951647314 118 48 -5.888274131574608 118 49 -4.3490225746310145 118 50 -0.4687171508719672 118 51 -3.6473531263762737 118 52 -3.328933690604675 118 53 -5.013557847115926 118 54 -8.746639512567357 118 55 -5.103357560201015 118 56 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7.188613384329436 119 40 10.460600338361264 119 41 6.631801708280349 119 42 3.52312474693095 119 43 -1.3195144441683944 119 44 -1.5849635013028767 119 45 0.2086788284605241 119 46 -6.716994045146954 119 47 -9.007892595164732 119 48 -9.78410746490794 119 49 -6.2240225746310145 119 50 -5.147883817538634 119 51 -6.297353126376272 119 52 -7.349767023938007 119 53 -7.946891180449258 119 54 -10.142472845900691 119 55 -10.032524226867682 119 56 -6.616717864296767 120 1 0.7670041446246074 120 2 2.057481699307239 120 3 2.5393329501074504 120 4 2.4204248205339596 120 5 2.77128524879528 120 6 1.2026256685417849 120 7 1.4108698980845062 120 8 1.0605768469055548 120 9 1.3186420695609513 120 10 1.4354483046755604 120 11 -9.691351861950764 120 12 -1.877630688032724 120 13 -2.6122794889379612 120 14 -1.7983664698505422 120 15 -7.205088559794454 120 16 -8.435290547807263 120 17 -10.050212871434269 120 18 -2.443182022024022 120 19 -1.5611385026598548 120 20 -1.9265154282096333 120 21 -0.4959697411072099 120 22 -0.15776066530029453 120 23 0.3091263252413583 120 24 -3.9453425146423875 120 25 0.0752084175222194 120 26 1.1468564656638165 120 27 0.6746580520689278 120 28 1.4825173219908194 120 29 1.0520936327044055 120 30 -0.6766088322454564 120 31 -2.80906378000482 120 32 -10.082410421730593 120 33 -2.363241939104654 120 34 -8.618759613195522 120 35 -2.6998511101039995 120 36 -0.8085944671386152 120 37 -0.7333883144356079 120 38 -0.32008971339895353 120 39 1.8729157099108313 120 40 -0.023120591871297336 120 41 -0.5292448033475594 120 42 -5.200712462371374 120 43 -3.6445144441683937 120 44 -6.856475129209855 120 45 -11.185507218051102 120 46 -12.793156835844629 120 47 -15.017776316094963 120 48 -12.316084209093987 120 49 -14.232743504863572 120 50 -11.43044195707352 120 51 -12.243283358934413 120 52 -11.62011586114731 120 53 -13.832356296728328 120 54 -11.793635636598365 120 55 -12.960431203611869 120 56 -10.331834143366535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1433.0 5 10.0 6 16.0 7 96.0 8 159.0 9 145.0 10 80.0 11 95.0 12 98.0 13 124.0 14 168.0 15 232.0 16 272.0 17 382.0 18 459.0 19 580.0 20 719.0 21 898.0 22 1024.0 23 1260.0 24 1402.0 25 1590.0 26 1745.0 27 1904.0 28 1972.0 29 2062.0 30 2358.0 31 2824.0 32 3407.0 33 4138.0 34 4802.0 35 5670.0 36 6344.0 37 5831.0 38 3214.0 39 887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.08390410958904 0.678082191780822 0.16267123287671234 0.07534246575342465 8 99.21404109589041 0.5308219178082192 0.14041095890410957 0.11472602739726029 9 96.47683480345978 2.1067054894236534 0.6611287145670978 0.7553309925494562 10 55.38869863013699 28.66267123287671 6.965753424657533 8.982876712328768 11 34.225487174218294 33.84876194390219 15.368677009486625 16.55707387239289 12 34.95205479452055 12.597602739726026 18.982876712328768 33.467465753424655 13 24.63013698630137 17.991438356164384 22.830479452054796 34.54794520547945 14 21.314311159827362 14.088511337946152 25.82722477221347 38.76995273001302 15 32.51376902355747 14.15163941458058 24.899198737196095 28.435392824665858 16 28.598348579847194 15.524034672970844 23.752012882447666 32.125603864734295 17 28.632354200565214 25.313008478205017 21.496959835574206 24.557677485655564 18 34.95898761922702 21.526790759799304 20.648320975392572 22.865900645581107 19 35.53016938702108 23.731310051895115 18.94599825303578 21.792522308048024 20 34.152064813387454 23.89736738434133 20.341537776407517 21.609030025863692 21 25.104889117218942 28.872334960184947 23.575648600051373 22.447127322544738 22 25.92808219178082 21.044520547945204 31.511986301369866 21.51541095890411 23 36.30650684931507 20.83219178082192 23.273972602739725 19.58732876712329 24 28.636986301369866 26.289383561643838 21.95376712328767 23.11986301369863 25 33.76712328767123 19.453767123287673 23.058219178082194 23.7208904109589 26 22.261865962227905 25.135084138120316 27.18493232927938 25.418117570372406 27 20.367896413523788 16.877333607371632 35.18651731579488 27.5682526633097 28 29.397842454593786 20.43836932100777 28.202445675476355 21.961342548922087 29 30.819559818446518 19.691701635694102 29.288344609060545 20.200393936798836 30 26.101480060298755 20.326846649307935 32.5322050157599 21.03946827463341 31 27.32109147073966 18.927406898918825 31.96327441221898 21.788227218122532 32 25.966330427634393 21.236149406586634 32.51528488979466 20.282235275984313 33 25.126067716373367 22.371445233439676 34.12575897910878 18.37672807107818 34 20.145339869057004 25.73098412916053 38.71216535837932 15.411510643403147 35 19.228001644736842 29.557291666666668 36.09854714912281 15.116159539473683 36 19.290393387522723 30.980553554892477 33.81006276365881 15.918990293925987 37 21.044048477357777 29.510419885329764 33.583959899749374 15.861571737563086 38 24.055212620027437 29.09122085048011 32.136488340192045 14.71707818930041 39 21.043672627235214 23.325309491059148 29.537482806052267 26.093535075653367 40 13.735014468788757 17.858619264158744 24.84842221303569 43.557944054016815 41 14.508801326676915 20.319922610513224 27.516453903159494 37.654822159650365 42 16.742174629324545 27.076468973091707 36.5561504667765 19.625205930807248 43 12.288237517611078 14.91185869901378 27.999037833751416 44.80086594962373 44 13.212515489467163 17.31722428748451 25.684978658956354 43.785281564091974 45 19.993485788733842 25.82884767031234 34.74474577433401 19.43292076661981 46 19.421834569974468 27.640214541528866 34.359202837705844 18.57874805079082 47 16.507664639890383 23.812623105249635 33.63192600839257 26.047786246467414 48 19.335880609290133 26.163836677346946 37.22392611757449 17.276356595788428 49 14.3411784916681 17.36127813090534 26.969592853461606 41.32795052396496 50 17.71106877435079 25.999347785043682 37.04752587405386 19.242057566551672 51 17.707509881422926 26.906684997422236 35.95119436329266 19.434610757862174 52 16.826253631534613 26.86905846555844 36.592116346633205 19.712571556273744 53 16.55026854505208 27.23544451498876 37.296017296703674 18.918269643255485 54 14.701646090534979 24.284979423868315 33.321330589849104 27.6920438957476 55 17.486788827122364 26.80667078443484 36.17802484386796 19.52851554457484 56 17.32079422010949 29.177463918587293 33.575878224159524 19.92586363714369 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 14.0 14 27.0 15 59.0 16 91.0 17 117.5 18 144.0 19 168.0 20 192.0 21 264.5 22 406.5 23 476.0 24 551.5 25 627.0 26 770.5 27 914.0 28 1140.0 29 1537.5 30 1709.0 31 1827.5 32 1946.0 33 2004.0 34 2062.0 35 1990.5 36 1919.0 37 1816.0 38 1727.0 39 1741.0 40 1750.5 41 1760.0 42 1591.0 43 1422.0 44 1309.0 45 1196.0 46 1155.0 47 1139.0 48 1164.0 49 1228.5 50 1293.0 51 1323.5 52 1354.0 53 1467.0 54 1751.0 55 1922.0 56 2136.0 57 2350.0 58 2617.0 59 2884.0 60 3295.0 61 3706.0 62 4043.0 63 4398.5 64 4417.0 65 4113.0 66 3809.0 67 3334.0 68 2859.0 69 2441.0 70 2023.0 71 1697.5 72 1150.0 73 928.0 74 804.5 75 681.0 76 547.5 77 414.0 78 341.0 79 200.5 80 133.0 81 99.5 82 66.0 83 47.0 84 28.0 85 18.5 86 9.0 87 5.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.025684931506849314 10 0.0 11 0.003424657534246575 12 0.0 13 0.0 14 0.020547945205479454 15 0.20034246575342465 16 0.04452054794520548 17 0.025684931506849314 18 0.005136986301369864 19 0.02226027397260274 20 0.02910958904109589 21 0.008561643835616438 22 0.0 23 0.0 24 0.0 25 0.0 26 0.17636986301369864 27 0.023972602739726026 28 0.15924657534246575 29 0.025684931506849314 30 0.04109589041095891 31 0.2226027397260274 32 0.015410958904109588 33 0.1678082191780822 34 0.09246575342465753 35 0.0547945205479452 36 0.14726027397260275 37 0.25 38 0.136986301369863 39 0.410958904109589 40 0.589041095890411 41 0.8750000000000001 42 0.2191780821917808 43 0.339041095890411 44 0.5068493150684932 45 0.113013698630137 46 0.07363013698630137 47 0.025684931506849314 48 0.06335616438356165 49 0.3253424657534247 50 0.23458904109589043 51 0.3595890410958904 52 0.3921232876712329 53 0.2106164383561644 54 0.136986301369863 55 0.19863013698630136 56 0.22089041095890413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 58400.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.71575342465754 #Duplication Level Percentage of deduplicated Percentage of total 1 93.13780975866112 78.90239726027397 2 4.416461171524437 7.482876712328768 3 1.0328657476654406 2.625 4 0.4931883413510127 1.6712328767123288 5 0.2546792254517524 1.0787671232876712 6 0.14957351336055302 0.7602739726027397 7 0.10106318470307636 0.5993150684931506 8 0.06063791082184582 0.410958904109589 9 0.05457411973966124 0.41609589041095896 >10 0.26882807131018316 3.8784246575342465 >50 0.0222339006346768 1.2928082191780823 >100 0.00808505477624611 0.8818493150684932 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA 157 0.2688356164383562 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 130 0.2226027397260274 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 125 0.21404109589041095 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 103 0.17636986301369864 No Hit AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAACCAAAAAAAAAAAAAAA 98 0.1678082191780822 No Hit AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAACCAAAACAAAAAAA 84 0.14383561643835616 No Hit AGGGTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 82 0.14041095890410957 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 74 0.1267123287671233 No Hit AGGGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAACCAAAAAAAAAAAAAAA 74 0.1267123287671233 No Hit AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACAAAAAAAAAAAAAAA 64 0.1095890410958904 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 61 0.10445205479452055 No Hit AGGGTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT 59 0.10102739726027397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.008561643835616438 0.0 0.0 0.0 0.0 11 0.017123287671232876 0.0 0.0 0.0 0.0 12 0.017123287671232876 0.0 0.0 0.0 0.0 13 0.0273972602739726 0.0 0.0 0.0 0.0 14 0.02910958904109589 0.0 0.0 0.0 0.0 15 0.032534246575342464 0.0 0.0 0.0 0.0 16 0.03595890410958904 0.0 0.0 0.0 0.0 17 0.03767123287671233 0.0 0.0 0.0 0.0 18 0.039383561643835614 0.0 0.0 0.0 0.0 19 0.04452054794520548 0.0 0.0 0.0 0.0 20 0.05136986301369863 0.0 0.0 0.0 0.0 21 0.05650684931506849 0.0 0.0 0.0 0.0 22 0.059931506849315065 0.0 0.0 0.0 0.0 23 0.06335616438356165 0.0 0.0 0.0 0.0 24 0.07876712328767123 0.0 0.0 0.0 0.0 25 0.07876712328767123 0.0 0.0 0.0 0.0 26 0.08904109589041095 0.0 0.0 0.0 0.0 27 0.08904109589041095 0.0 0.0 0.0 0.0 28 0.09075342465753425 0.0 0.0 0.0 0.0 29 0.09246575342465753 0.0 0.0 0.0 0.0 30 0.09417808219178082 0.0 0.0 0.0 0.0 31 0.09417808219178082 0.0 0.0 0.0 0.0 32 0.09417808219178082 0.0 0.0 0.0 0.0 33 0.09417808219178082 0.0 0.0 0.0 0.0 34 0.09417808219178082 0.0 0.0 0.0 0.0 35 0.09417808219178082 0.0 0.0 0.0 0.0 36 0.09417808219178082 0.0 0.0 0.0 0.0 37 0.0958904109589041 0.0 0.0 0.0 0.0 38 0.0958904109589041 0.0 0.0 0.0 0.0 39 0.0958904109589041 0.0 0.0 0.0 0.0 40 0.0958904109589041 0.0 0.0 0.0 0.0 41 0.0958904109589041 0.0 0.0 0.0 0.0 42 0.09931506849315068 0.0 0.0 0.0 0.0 43 0.10445205479452055 0.0 0.0 0.0 0.0 44 0.1095890410958904 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACC 25 1.9608358E-5 50.429688 38 CCAGCGT 15 0.008111619 50.429688 39 CAACCAA 30 9.714258E-7 50.429688 40 TCTGGCC 15 0.008111619 50.429688 38 TTCCCGG 15 0.008111619 50.429688 41 ATTCCCG 15 0.008111619 50.429688 40 CATATTG 15 0.008111619 50.429688 40 AAACCAC 15 0.008195544 50.298702 37 TACAAAC 20 4.030507E-4 50.2987 37 AAGGCAT 15 0.0082518505 50.211754 35 CCGCTCT 30 1.0069507E-6 50.168392 48 AAAGGCA 15 0.008280112 50.168392 34 CTCGCGC 15 0.008280112 50.168392 49 ACCGATC 20 4.099968E-4 50.125107 50 TAGCAAG 15 0.008308445 50.125107 47 ACGGGCA 20 4.099968E-4 50.125107 44 TACGAAG 15 0.008308445 50.125107 47 CTACCAA 15 0.008308445 50.125107 46 CAAAGGC 20 4.117481E-4 50.081898 33 CCTAGCA 15 0.00833685 50.081898 45 >>END_MODULE