FastQCFastQC Report
Fri 17 Jun 2016
SRR1526281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32927
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA360.10933276642269261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGC150.00819834650.2450239
AAGACCT150.00819834650.2450236
AGGCTAG150.00819834650.2450237
GGCTAGG150.00819834650.2450238
AGACCCT150.00819834650.2450240
TGAAAAC150.00819834650.2450234
CAAAACC150.00819834650.2450234
TGCGCTT204.0563062E-450.16819449
CCCCGAC150.00824819550.16819441
TATTTTA150.00824819550.16819448
GTAGGGG204.0563062E-450.16819448
TAGGGGT204.0563062E-450.16819449
GTGCGCT204.0563062E-450.16819448
ATTTTAT150.00824819550.16819449
TTTAAAT150.00824819550.16819448
TAGGCTC204.0563062E-450.16819441
AGGGGTT204.0870943E-450.09160250
CAGTCAT150.008298269550.09160233
GTGCAAG150.008298269550.09160232
GTGTAGG204.0870943E-450.09160246