##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526281_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32927 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.02873022139885 39.0 38.0 40.0 35.0 40.0 2 37.70039784978893 39.0 38.0 40.0 35.0 40.0 3 37.48653081058098 39.0 38.0 40.0 34.0 40.0 4 36.14097852825948 38.0 35.0 39.0 31.0 40.0 5 37.74987092659519 39.0 38.0 40.0 35.0 40.0 6 37.605156862149606 38.0 38.0 40.0 35.0 40.0 7 37.44495398912746 38.0 38.0 40.0 35.0 40.0 8 37.225589941385486 38.0 38.0 40.0 33.0 40.0 9 37.204634494487806 38.0 38.0 40.0 33.0 40.0 10 36.8755428675555 38.0 37.0 40.0 33.0 40.0 11 35.66975430497768 38.0 35.0 39.0 29.0 40.0 12 33.60491390044644 38.0 32.0 39.0 20.0 40.0 13 33.098065417438576 38.0 31.0 39.0 18.0 40.0 14 32.73638655206973 37.0 30.0 39.0 16.0 40.0 15 33.14386369848452 38.0 31.0 39.0 18.0 40.0 16 32.77535153521426 37.0 30.0 39.0 18.0 40.0 17 32.870410301576214 36.0 30.0 39.0 19.0 40.0 18 33.00877699152671 36.0 30.0 39.0 20.0 40.0 19 33.80802988428949 37.0 31.0 39.0 24.0 40.0 20 33.95031433170347 37.0 31.0 39.0 25.0 40.0 21 35.26233789898867 38.0 35.0 39.0 27.0 40.0 22 34.8780939654387 38.0 34.0 39.0 25.0 40.0 23 35.08974397910529 38.0 35.0 39.0 27.0 40.0 24 34.65772770067118 38.0 33.0 39.0 25.0 40.0 25 34.88744799101042 38.0 34.0 39.0 26.0 40.0 26 35.26209493728551 38.0 35.0 39.0 27.0 40.0 27 35.195918243386885 38.0 35.0 39.0 27.0 40.0 28 35.12330306435448 38.0 35.0 39.0 27.0 40.0 29 34.96628906368634 38.0 35.0 39.0 27.0 40.0 30 34.79888845020803 38.0 35.0 39.0 26.0 40.0 31 34.535092781000394 38.0 34.0 39.0 26.0 40.0 32 33.45743614662739 38.0 33.0 39.0 18.0 40.0 33 34.576730342879706 38.0 34.0 39.0 26.0 40.0 34 34.3468278312631 38.0 34.0 39.0 25.0 40.0 35 34.31861390348347 38.0 34.0 39.0 25.0 40.0 36 32.181158319919824 35.0 31.0 38.0 20.0 39.0 37 31.927567042244966 35.0 31.0 38.0 18.0 39.0 38 31.286573328879037 35.0 30.0 38.0 11.0 39.0 39 29.284842226744008 34.0 27.0 38.0 4.0 39.0 40 25.077109970540892 31.0 11.0 37.0 4.0 39.0 41 21.787013696966017 25.0 9.0 33.0 4.0 37.0 42 25.450693959364656 30.0 20.0 34.0 4.0 37.0 43 21.433261457162814 25.0 8.0 33.0 4.0 36.0 44 22.467427946669908 27.0 9.0 33.0 4.0 36.0 45 25.120812706897077 30.0 20.0 34.0 4.0 37.0 46 25.1212986303034 30.0 14.0 36.0 4.0 38.0 47 24.414067482613053 30.0 8.0 35.0 4.0 38.0 48 24.38521578036262 30.0 10.0 35.0 4.0 38.0 49 21.770796003279983 26.0 4.0 35.0 4.0 38.0 50 24.090563974853463 29.0 4.0 35.0 4.0 38.0 51 25.622893066480398 32.0 4.0 38.0 4.0 40.0 52 25.410605278343002 32.0 4.0 38.0 4.0 40.0 53 25.024053208612994 31.0 4.0 37.0 4.0 39.0 54 24.253591277674857 31.0 4.0 37.0 4.0 39.0 55 24.41054453791721 31.0 4.0 37.0 4.0 39.0 56 24.032010204391533 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.18781491127064953 1 2 0.34974663535832917 1 3 0.5588544230149282 1 4 -0.9121021035569896 1 5 0.2716712516522932 1 6 0.5922510753898962 1 7 0.7165341923378534 1 8 1.2254935017777484 1 9 0.9579246377654371 1 10 1.0957388822376757 1 11 -4.134828829021281 1 12 -7.731712817343666 1 13 -20.82766550289564 1 14 -18.998549583477505 1 15 -12.84160546473312 1 16 -22.298445847173696 1 17 -21.860203401237328 1 18 -20.66111221836627 1 19 -6.530057565623149 1 20 -11.790376779613663 1 21 -7.2579937748291385 1 22 -2.454427103127969 1 23 -3.202725552671172 1 24 -3.3471694943129897 1 25 -4.51286893939017 1 26 1.5649009544347763 1 27 2.2224784803816746 1 28 1.8386565993849473 1 29 2.0500359755514452 1 30 1.965362569992493 1 31 0.6897976886413417 1 32 -15.311490061306262 1 33 -0.3859990528944479 1 34 -0.7706221067344075 1 35 0.4284905648157391 1 36 1.3207614272794466 1 37 0.5779500311431498 1 38 2.3098877771712907 1 39 4.519114395061443 1 40 7.498029203225812 1 41 -6.432794758104677 1 42 -12.584818385066319 1 43 -13.054873210430982 1 44 -16.426700480517713 1 45 -16.420278214373106 1 46 -18.82807094315268 1 47 -18.275986347274568 1 48 -18.085415444199306 1 49 -14.914757693217197 1 50 -17.72225240146999 1 51 -19.07991411275147 1 52 -18.582468965468635 1 53 -19.139088028801737 1 54 -18.19836406978271 1 55 -18.48540828650191 1 56 -18.235189687283498 2 1 -0.0016677251234682444 2 2 0.33243061804231644 2 3 0.7450016091621094 2 4 -0.37314106459595564 2 5 0.3972123771934193 2 6 0.6701731533119784 2 7 1.0238934996971594 2 8 1.3618571381413886 2 9 1.3561930360338295 2 10 0.7321025186013088 2 11 -4.078551772744223 2 12 -14.244699830330681 2 13 -13.80602048125062 2 14 -17.5937876787156 2 15 -10.975804598932253 2 16 -5.69238524111309 2 17 -4.364532405566333 2 18 -12.933839491093543 2 19 -3.391529427095012 2 20 -4.366134355371241 2 21 1.5515300346946717 2 22 2.8334516847508198 2 23 2.3059324559868344 2 24 1.0424408952973963 2 25 2.2360488095275812 2 26 1.6558100453438698 2 27 2.5969373548405486 2 28 1.5529423136706626 2 29 2.2448411703566364 2 30 2.205622310252238 2 31 -2.046133047289395 2 32 -10.837464087280289 2 33 -1.4985531654485627 2 34 -1.0996264357387346 2 35 -1.409171772846598 2 36 4.045869652387669 2 37 4.883144836337955 2 38 6.017679984963493 2 39 8.164136040083086 2 40 9.296730501927115 2 41 5.359413034103117 2 42 1.5645322642843311 2 43 -3.0743537299115005 2 44 -8.357436411253643 2 45 -0.4592392533341467 2 46 -12.198200813282552 2 47 -12.403691974980196 2 48 -10.022644881428743 2 49 -10.693978472437976 2 50 -9.826148505366094 2 51 -9.913247446084805 2 52 -9.924460307459976 2 53 -9.9009927907065 2 54 -14.50788787930652 2 55 -10.866360667454293 2 56 -9.806618258712069 3 1 0.7007132272574879 3 2 0.6419544275661266 3 3 1.018811132971635 3 4 0.37685893540404436 3 5 0.653164758145806 3 6 1.2773160104548325 3 7 1.6012744520781155 3 8 1.5999523762366223 3 9 1.6121454169862162 3 10 0.7856739471727394 3 11 0.7607339415414884 3 12 1.1898239791931253 3 13 -5.7048300050601455 3 14 -5.933073393001312 3 15 -4.4400903132179685 3 16 1.8373766636488114 3 17 2.439039023005094 3 18 1.6673509850969346 3 19 1.0965658110002252 3 20 0.5505323112954272 3 21 1.5634347965994309 3 22 1.9941659704651045 3 23 2.5559324559868344 3 24 2.721012323868827 3 25 1.6110488095275812 3 26 1.7986671882010157 3 27 1.9659849738881618 3 28 1.2136565993849473 3 29 2.048412598928067 3 30 0.8544318340617636 3 31 1.2098193336629848 3 32 -0.21841646823266814 3 33 0.25739921550381695 3 34 1.4182307071184042 3 35 0.7872567985819785 3 36 3.0577744142924352 3 37 2.7521924553855754 3 38 3.535537127820639 3 39 6.056993182940232 3 40 7.915778120974728 3 41 6.883222557912639 3 42 5.594294169046236 3 43 0.11017007961230973 3 44 0.8151826363654067 3 45 4.469332175237284 3 46 -3.7041531942349337 3 47 -5.981072927361147 3 48 -2.8916925004763634 3 49 -10.033264186723692 3 50 -3.2963866006041904 3 51 -3.6037236365609964 3 52 -3.454222212221879 3 53 -4.264088028801737 3 54 -5.859078355496994 3 55 -3.6104082865019116 3 56 -1.3959039729977825 4 1 1.0882793648236273 4 2 1.2874570730687722 4 3 1.4645783287388312 4 4 1.584530893076007 4 5 1.061233541214584 4 6 1.6225541056929274 4 7 1.9471739229775835 4 8 2.121116397400648 4 9 2.3350290148698107 4 10 1.7135840000827898 4 11 3.1463159521235013 4 12 3.98545889982805 4 13 5.477048301818165 4 14 3.112561527633609 4 15 6.124724501596848 4 16 6.826133277405425 4 17 5.89737235633843 4 18 6.3439382866842315 4 19 4.246036710471124 4 20 4.9671989779620915 4 21 3.501265484430121 4 22 4.351970203269339 4 23 3.856197006251385 4 24 3.9154567683132697 4 25 3.1487472222259925 4 26 3.1558100453438698 4 27 2.2292124871156815 4 28 2.4127306734590235 4 29 3.2369046624201303 4 30 3.716204320834244 4 31 2.9776764765201307 4 32 3.845075595259395 4 33 3.1998595329641333 4 34 2.694024357912056 4 35 3.241621877947054 4 36 3.4268220333400556 4 37 4.242933196126316 4 38 5.020325487609 4 39 8.158845034792087 4 40 10.114190819387431 4 41 5.930841605531686 4 42 8.204743904495974 4 43 7.594958439400667 4 44 11.637272583455353 4 45 7.712718418623524 4 46 7.407619292537554 4 47 7.6465725752843525 4 48 6.837143478359618 4 49 3.2451749667154637 4 50 7.3854917062741166 4 51 5.957122924285564 4 52 7.266015883016216 4 53 6.509060119346412 4 54 0.9683025968839587 4 55 7.0701472690536455 4 56 4.912958460864651 5 1 1.0023849597802865 5 2 1.1389149138883141 5 3 1.6213947195977738 5 4 1.2058862910271486 5 5 1.401265062097174 5 6 1.7643980773241381 5 7 1.985392993111546 5 8 2.1242684856591154 5 9 1.9559904017886467 5 10 1.9023152845587532 5 11 3.286189868593162 5 12 6.122574739071549 5 13 6.044030177310674 5 14 4.866698643472851 5 15 6.395016069760754 5 16 6.768607666688325 5 17 6.79808157619658 5 18 6.101621501814254 5 19 4.7948940784774265 5 20 5.40691529001883 5 21 3.422857289000646 5 22 4.4398346634742225 5 23 3.4594278967163206 5 24 3.7263314728050005 5 25 3.2846810283725603 5 26 2.549427066620467 5 27 2.518416584830412 5 28 3.2003587270445237 5 29 3.2144460335785254 5 30 2.746149159289722 5 31 3.5840594552435334 5 32 4.20559568982204 5 33 3.5450131972667336 5 34 3.5356319229238764 5 35 3.1789741238099367 5 36 4.4232759340492755 5 37 3.938362668151534 5 38 5.839868434811521 5 39 6.349546374429593 5 40 8.090550157448895 5 41 3.0017635913472915 5 42 7.92066195020125 5 43 12.180458833411702 5 44 11.650668958553855 5 45 9.014924880404454 5 46 11.52267051397175 5 47 10.998030416103898 5 48 10.131863730526675 5 49 11.299942500206399 5 50 10.58013315623472 5 51 9.70022773122016 5 52 9.024881131243166 5 53 9.75452899247486 5 54 9.886742313196013 5 55 10.280549160306602 5 56 9.232895419099481 6 1 0.5164275129717737 6 2 1.0133829989946932 6 3 1.1230968472573508 6 4 0.16971607826118884 6 5 1.0753076152886578 6 6 1.5358874390262613 6 7 1.7819887377924033 6 8 1.9485238048080546 6 9 1.6942882741290717 6 10 1.252102518601312 6 11 3.5300196558272035 6 12 5.60768112205027 6 13 5.784455709225568 6 14 5.974783749855831 6 15 6.3099096867820315 6 16 6.0209480922202445 6 17 6.544039023005091 6 18 5.67949384223979 6 19 4.480851525285942 6 20 4.760532311295428 6 21 3.162006225170863 6 22 3.2134516847508223 6 23 3.5487895988439817 6 24 3.531012323868829 6 25 3.3931916666704325 6 26 2.0558100453438684 6 27 2.3588421167453077 6 28 1.5386565993849501 6 29 3.4191268846423526 6 30 3.3265746912046197 6 31 3.1176764765201312 6 32 3.6072978174816157 6 33 2.890970644075246 6 34 2.491802135689838 6 35 1.3393996557248329 6 36 2.9734887000067225 6 37 2.4621924553855763 6 38 3.5862514135349244 6 39 4.466993182940229 6 40 2.818635263831876 6 41 0.7775082721983537 6 42 5.812151311903378 6 43 10.860884365326594 6 44 9.378754064936835 6 45 5.497903603808712 6 46 8.857989662907922 6 47 10.152498501210278 6 48 8.70973607095221 6 49 9.908878670419167 6 50 8.692899113681523 6 51 7.47341922058186 6 52 8.319349216349547 6 53 7.300911971198264 6 54 7.921635930217292 6 55 7.814591713498089 6 56 7.664810312716501 7 1 0.8486724109309591 7 2 1.2235870806273468 7 3 1.6565662350124555 7 4 0.4942058741795563 7 5 1.043470880594782 7 6 1.7109894798425884 7 7 1.9150499622821968 7 8 2.120360539501931 7 9 2.328982151680094 7 10 1.4463882328870241 7 11 3.4928767986843496 7 12 5.3097219383768 7 13 6.323231219429651 7 14 6.375600076386441 7 15 6.207868870455503 7 16 7.089927684056974 7 17 6.400773716882647 7 18 6.930106087137748 7 19 5.3363617293675745 7 20 5.116858841907671 7 21 3.354251123130048 7 22 3.4763088276079657 7 23 3.561034496803167 7 24 3.7567266095831116 7 25 3.33808962585411 7 26 1.9415243310581545 7 27 3.6751686473575518 7 28 2.8386565993849473 7 29 3.1019840274994976 7 30 3.249839997327065 7 31 4.0491050479487 7 32 4.628522307277535 7 33 3.2701543175446375 7 34 2.7167000948735094 7 35 3.131644553684019 7 36 2.1241009449046793 7 37 2.5328047002835348 7 38 3.5993126380247205 7 39 5.164136040083086 7 40 -3.9107524912701663 7 41 -9.871879482903687 7 42 4.78562069965848 7 43 7.847414977571493 7 44 7.424876513916427 7 45 3.908107685441365 7 46 6.655540683316087 7 47 8.511274011414361 7 48 7.42973607095221 7 49 10.816225609194678 7 50 7.466368501436627 7 51 8.579949832826756 7 52 9.517716563288321 7 53 11.146626256912548 7 54 11.046533889400965 7 55 9.616632529824617 7 56 10.540320516798136 8 1 0.5949180790095099 8 2 0.015269791447529713 8 3 1.209511941596972 8 4 0.5716028707140239 8 5 1.0881378039679035 8 6 1.5030572503470196 8 7 1.65821515288674 8 8 1.5473917293363542 8 9 1.5188165760158654 8 10 1.0528572355824437 8 11 3.3500196558272037 8 12 5.717115084314425 8 13 6.1957764639425505 8 14 5.900821485704888 8 15 5.857079498102788 8 16 5.716419790333447 8 17 4.922529589042831 8 18 4.946663653560545 8 19 3.6551911479274466 8 20 4.54581533016335 8 21 1.9684213195104832 8 22 2.7108101753168583 8 23 2.326148089410019 8 24 2.565351946510333 8 25 1.8667765723308136 8 26 1.4859987245891517 8 27 1.8211062676887053 8 28 1.1216754673094727 8 29 2.468560846906506 8 30 1.1243105402612201 8 31 1.2889972312371114 8 32 1.6616374401231226 8 33 1.0090838516224139 8 34 -0.018763902046011083 8 35 -0.12210977823743008 8 36 0.2727339830255886 8 37 0.09653207802708508 8 38 1.663232545610395 8 39 1.759823371619479 8 40 -2.4036288871115232 8 41 -8.987397388179005 8 42 2.848000368507151 8 43 9.256733421930367 8 44 7.571584253616081 8 45 5.397903603808711 8 46 7.7402538138513215 8 47 7.459290954040469 8 48 6.599547391706928 8 49 9.920576783626714 8 50 7.340068925002278 8 51 7.781721107374313 8 52 8.806896386160869 8 53 8.672232725915244 8 54 9.650692533990874 8 55 8.288176619158467 8 56 7.161036727810842 9 1 1.0119377170534065 9 2 0.8562401418518419 9 3 0.9830968472573502 9 4 0.6574711803020037 9 5 0.9006137377376362 9 6 1.588540500250751 9 7 2.078315268404644 9 8 2.120360539501931 9 9 2.4106148047413143 9 10 1.1606739471727394 9 11 2.145938023174139 9 12 6.003599489397207 9 13 5.813027137797 9 14 5.987844974345627 9 15 5.9425627480065195 9 16 6.518499112628405 9 17 4.176283920964281 9 18 5.950514250403057 9 19 5.254729076306347 9 20 4.402573127621956 9 21 2.7420062251708615 9 22 3.598757807199796 9 23 2.3161365376194922 9 24 3.246522527950461 9 25 2.37890595238472 9 26 2.145605963711219 9 27 2.30782170858204 9 28 2.124370885099232 9 29 2.040759537703579 9 30 1.9641257116127804 9 31 2.232778517336456 9 32 3.281583531767332 9 33 2.5354604399936136 9 34 2.9003735642612654 9 35 1.8051139414391173 9 36 1.3485907408230453 9 37 2.0021924553855754 9 38 1.4768636584328831 9 39 5.143727876817785 9 40 0.23210465158697602 9 41 -4.9126958094343 9 42 4.703988046597257 9 43 9.418843549000062 9 44 8.322835697589895 9 45 4.724434216053609 9 46 7.96166313229568 9 47 8.817396460393955 9 48 8.327695254625677 9 49 11.448878670419166 9 50 8.262286868783566 9 51 7.641174322622678 9 52 7.660573706145467 9 53 7.371116052830917 9 54 7.8628604200132095 9 55 6.657448856355231 9 56 5.805626639247116 10 1 -0.5635724870282246 10 2 -0.24217255656085968 10 3 0.005319069479575944 10 4 0.38082718937230453 10 5 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2.3480076811122856 114 34 1.8792095430972395 114 35 2.667547803872978 114 36 5.000896107414125 114 37 4.705896159089278 114 38 4.983288450571962 114 39 8.195882071829118 114 40 11.373450078646691 114 41 7.708619383309465 114 42 3.2973364970885655 114 43 1.5208843653265944 114 44 -2.659023712840945 114 45 3.731236937142043 114 46 -3.036825151906889 114 47 -6.6497237210119415 114 48 -3.4221157808996416 114 49 -13.47704725550676 114 50 -2.9108045900221775 114 51 -3.0428770757144363 114 52 -2.8450952280948947 114 53 -2.9909398806535883 114 54 -3.827993699412339 114 55 -2.3002231013167247 114 56 -0.6425970946909061 115 1 -0.03023915369489316 115 2 0.6133829989946946 115 3 0.9497635139240188 115 4 0.8363827449278602 115 5 0.4686409486219958 115 6 0.8225541056929302 115 7 0.895322071125733 115 8 1.3618571381413886 115 9 0.8942882741290674 115 10 0.532102518601306 115 11 -0.4499803441727934 115 12 -5.258985544616394 115 13 0.05112237589223767 115 14 -7.131882916810834 115 15 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115 54 0.06830259688395657 115 55 1.1812583801647563 115 56 5.598143646049834 116 1 0.16976084630510258 116 2 0.1800496656613646 116 3 0.5164301805906817 116 4 0.7030494115945203 116 5 0.4686409486219958 116 6 0.8892207723595931 116 7 0.895322071125733 116 8 1.4951904714747215 116 9 1.3276216074624045 116 10 0.8654358519346417 116 11 -2.3833136775061305 116 12 -3.625652211283061 116 13 -10.382210957441096 116 14 -12.298549583477502 116 15 -5.2234236465513035 116 16 -10.125718574446424 116 17 -10.902627643661571 116 18 -6.533839491093545 116 19 -5.9391484747140595 116 20 -6.099467688704571 116 21 0.8086728918375314 116 22 0.9334516847508212 116 23 0.6154562655106446 116 24 1.3710123238688254 116 25 1.1931916666704367 116 26 2.4891433786772055 116 27 2.8255087834119763 116 28 2.3719899327182787 116 29 2.5257935513090217 116 30 2.6532413578712877 116 31 -0.7556568568132036 116 32 -6.232702182518384 116 33 0.4443039774085804 116 34 0.2718021356898319 116 35 0.4193996557248312 116 36 3.16015536667339 116 37 3.602192455385577 116 38 4.2795847468682595 116 39 6.840326516273564 116 40 8.125301930498544 116 41 4.130841605531689 116 42 1.0454846452367121 116 43 -10.545782301340072 116 44 -0.147912601729832 116 45 0.9645702704753774 116 46 -7.65534367042541 116 47 -10.52750149878972 116 48 -9.17026392904779 116 49 -9.417787996247501 116 50 -6.973767552985143 116 51 -3.2465807794181387 116 52 -2.6673174503171175 116 53 -5.505754695468404 116 54 -5.431697403116043 116 55 -3.9854082865019116 116 56 -1.4018563539501656 117 1 -0.8812195458517564 117 2 -0.521911118652362 117 3 -0.6051384468602947 117 4 -0.8479309805623387 117 5 -0.8941041494172239 117 6 -0.573524325679621 117 7 -1.7968347916193679 117 8 -1.177358548133121 117 9 -1.8762999611650457 117 10 -0.3855445402222202 117 11 -9.29703916770221 117 12 -10.6746718191262 117 13 -10.380250173127372 117 14 -7.018157426614756 117 15 -9.278325607335617 117 16 -13.300228378367994 117 17 -8.730078624053728 117 18 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39 8.567862748157623 118 40 9.080374394266656 118 41 8.684464793937483 118 42 5.155629572772945 118 43 4.433927843587465 118 44 -3.8377676741936 118 45 4.571816647286973 118 46 -5.048097293613814 118 47 -9.862284107485372 118 48 -4.526785668178224 118 49 -7.203295242624312 118 50 -2.163622625448909 118 51 -2.4422329533311817 118 52 -1.4876073053895809 118 53 -2.8782184635843464 118 54 -7.198364069782709 118 55 -4.876712634327998 118 56 -0.3221462090226268 119 1 -0.459224660941274 119 2 -0.4170517836139993 119 3 -0.36472923969917304 119 4 0.008846513043799575 119 5 -0.40817064558090266 119 6 -0.5223734305389556 119 7 -0.2597503926423812 119 8 0.006784674373271571 119 9 -0.4491899867404925 119 10 -0.22441922052912844 119 11 -13.649980344172796 119 12 -7.3575362692540764 119 13 -4.9633703777309535 119 14 -4.013042337100693 119 15 -4.646612052348402 119 16 -4.141660603431934 119 17 -9.870743585690558 119 18 -3.07876702732543 119 19 -2.004365866018407 119 20 -1.0038155147915298 119 21 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120 3 -0.17690315274264634 120 4 -0.5302839217388069 120 5 0.27530761528866066 120 6 -0.0041125609737378 120 7 0.06198873779239733 120 8 0.28852380480805095 120 9 0.19428827412907168 120 10 -0.3478974813986895 120 11 -8.929980344172797 120 12 -3.7923188779497288 120 13 -5.855544290774429 120 14 -5.665216250144169 120 15 -4.6900903132179685 120 16 -12.319051907779759 120 17 -7.755960976994906 120 18 -4.360506157760213 120 19 -3.91914847471406 120 20 -4.899467688704572 120 21 -0.5379937748291397 120 22 -0.5465483152491828 120 23 -1.611210401156022 120 24 -4.728987676131172 120 25 -1.506808333329566 120 26 0.29581004534387034 120 27 1.5388421167453075 120 28 0.5986565993849453 120 29 0.19912688464235373 120 30 -0.593425308795382 120 31 -4.522323523479869 120 32 -8.872702182518385 120 33 -5.889029355924752 120 34 -8.888197864310165 120 35 -4.400600344275169 120 36 -3.1665112999932816 120 37 -2.1578075446144247 120 38 -1.613748586465075 120 39 0.9069931829402336 120 40 -4.001364736168124 120 41 -0.36249172780164685 120 42 -4.807848688096623 120 43 -2.719115634673404 120 44 -6.801245935063166 120 45 -10.642096396191288 120 46 -12.702010337092076 120 47 -14.82750149878972 120 48 -11.61026392904779 120 49 -14.911121329580833 120 50 -11.287100886318475 120 51 -10.426580779418138 120 52 -10.040650783650452 120 53 -11.539088028801737 120 54 -12.558364069782709 120 55 -12.205408286501912 120 56 -9.795189687283498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 97.0 5 31.0 6 36.0 7 41.0 8 95.0 9 123.0 10 85.0 11 83.0 12 79.0 13 72.0 14 76.0 15 123.0 16 180.0 17 232.0 18 256.0 19 343.0 20 430.0 21 506.0 22 671.0 23 754.0 24 865.0 25 950.0 26 974.0 27 1079.0 28 1188.0 29 1193.0 30 1347.0 31 1563.0 32 1782.0 33 2252.0 34 2742.0 35 3233.0 36 3627.0 37 3313.0 38 1908.0 39 598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.41992893370183 0.2399246818720199 0.27940595863577006 0.060740425790384786 8 99.33489233759528 0.4039238315060588 0.13970297931788503 0.12148085158076957 9 98.07406057292141 1.111819921625809 0.37060664054193626 0.4435128649108418 10 47.33501381844687 37.17010356242597 5.882710237798767 9.612172381328394 11 29.510735870258454 30.804506939593644 20.758040513864 18.9267166762839 12 33.085309928022596 16.54872900659034 22.16721839220093 28.19874267318614 13 25.140462234640264 14.246666869134753 24.99772223403286 35.61514866219212 14 22.8476821192053 17.847378334042165 25.742754723859285 33.56218482289325 15 25.750616007057463 17.960027986493472 24.768046725276065 31.521309281173 16 27.296476306196844 19.86026731470231 25.826245443499392 27.01701093560146 17 30.71992709599028 20.85662211421628 22.828068043742405 25.595382746051033 18 29.287406597412062 24.27556041552761 22.401433691756274 24.035599295304053 19 27.9951397326853 24.562575941676794 21.123936816524907 26.318347509113 20 26.94352462253547 25.934927241243127 23.513685937357597 23.607862198863806 21 28.94417107101634 21.82431201020594 25.444991191300648 23.786525727477066 22 31.797612901266437 22.622771585628815 21.64788775169314 23.931727761411608 23 29.811400977920854 24.590761381237282 23.813283931120356 21.784553709721504 24 27.062896710905942 24.888389467610168 23.52476690861603 24.52394691286786 25 25.81850209560833 26.416813460487155 24.9559618538541 22.808722590050415 26 25.88457208920259 25.130061760321276 26.599531473424808 22.385834677051324 27 26.271340907710066 20.54195273102862 27.635336290175587 25.551370071085728 28 27.068309752015825 24.232466149399055 26.322835843602615 22.376388254982505 29 27.05453440680541 23.378398906273734 26.735530912957618 22.83153577396324 30 24.795648606764107 25.91996110486493 26.217751982740285 23.06663830563068 31 23.84329721173749 26.591988311213928 27.289053938877387 22.27566053817119 32 23.48652835576076 23.914826402600163 28.57446614622885 24.024179095410226 33 23.87632756154712 26.149538967164727 29.433066553056815 20.541066918231337 34 22.738326848249027 25.957563229571985 30.654182879377434 20.649927042801554 35 21.942620957938246 28.7290299051787 31.190736688548505 18.13761244833455 36 22.074840279890477 28.177669607544875 29.072102220870093 20.675387891694555 37 20.951336865826175 27.998051038431086 32.11218710031062 18.938424995432122 38 24.90265271355561 28.206376247262106 29.9190800681431 16.971890971039183 39 20.676622980798538 23.916488875342885 27.436757086254193 27.97013105760439 40 15.040488922841863 17.683728036669212 23.618029029793735 43.657754010695186 41 16.819337642841823 20.578413651542537 25.780460157470664 36.82178854814497 42 21.5980024968789 27.77016534210286 32.529460126061934 18.102372034956304 43 13.942571480826679 16.58538072303847 25.571541791135765 43.90050600499909 44 15.12199590802211 18.35587992793233 23.84340550279415 42.678718661251416 45 20.81508515815085 28.427615571776155 32.61557177615572 18.141727493917276 46 20.701605058365757 28.25875486381323 32.09812743190661 18.941512645914397 47 18.550592525068367 24.92555454269219 30.786994834396843 25.736858097842603 48 21.8886728245017 27.643412736995625 33.164195430238216 17.303719008264462 49 14.68256388363725 18.094773434164786 24.352015612612064 42.8706470695859 50 19.64312901555982 28.708017417252822 32.68779878810024 18.961054779087117 51 20.67304761324148 30.13168322867768 31.018716088520392 18.17655306956045 52 20.010979961570136 31.30204044285845 30.365693720071977 18.321285875499434 53 19.071395950677424 31.42335210838788 31.17978383315573 18.32546810777896 54 17.92820200790995 26.823851536355335 28.0985701247338 27.149376331000912 55 20.348270823185583 29.563443740867022 31.779712615684364 18.30857282026303 56 19.096915628901133 30.648844502633743 31.684072709557594 18.57016715890753 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 10.0 13 56.5 14 95.0 15 120.5 16 146.0 17 153.5 18 161.0 19 181.0 20 201.0 21 220.5 22 270.5 23 301.0 24 346.0 25 391.0 26 479.5 27 568.0 28 671.5 29 867.5 30 960.0 31 1060.0 32 1160.0 33 1128.5 34 1097.0 35 1091.0 36 1085.0 37 1064.0 38 934.5 39 826.0 40 804.0 41 782.0 42 796.0 43 810.0 44 762.0 45 714.0 46 766.5 47 848.0 48 877.0 49 904.5 50 932.0 51 944.5 52 957.0 53 1098.0 54 1348.5 55 1458.0 56 1585.5 57 1713.0 58 1816.0 59 1919.0 60 2013.5 61 2108.0 62 2053.0 63 1871.5 64 1745.0 65 1639.5 66 1534.0 67 1392.5 68 1251.0 69 1109.5 70 968.0 71 857.5 72 626.0 73 505.0 74 433.5 75 362.0 76 297.0 77 232.0 78 170.5 79 78.5 80 48.0 81 34.5 82 21.0 83 15.0 84 9.0 85 4.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.024296170316153918 10 0.0 11 0.0 12 0.0 13 0.0 14 0.027333191605673154 15 0.16399914963403894 16 0.021259149026634675 17 0.021259149026634675 18 0.015185106447596197 19 0.021259149026634675 20 0.030370212895192393 21 0.015185106447596197 22 0.0 23 0.0 24 0.0 25 0.0030370212895192397 26 0.1761472347921159 27 0.027333191605673154 28 0.18829531995019286 29 0.03644425547423088 30 0.05466638321134631 31 0.22777659671394296 32 0.01822212773711544 33 0.20044340510826983 34 0.09414765997509643 35 0.06985148965894251 36 0.17311021350259664 37 0.2702948947672123 38 0.16703617092355816 39 0.35533149087375104 40 0.6134783004828863 41 0.8685880888025025 42 0.2611838308986546 43 0.367479576031828 44 0.5466638321134631 45 0.14274000060740427 46 0.09414765997509643 47 0.05162936192182708 48 0.04555531934278859 49 0.4039238315060588 50 0.2611838308986546 51 0.367479576031828 52 0.42518298053269354 53 0.24903574574057763 54 0.17311021350259664 55 0.2399246818720199 56 0.2551097883196161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 32927.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.64679442402891 #Duplication Level Percentage of deduplicated Percentage of total 1 93.97316891388306 84.24393354997419 2 4.150010163290196 7.440702159322137 3 0.9214716444203537 2.4782093722476994 4 0.3455518666576326 1.2391046861238497 5 0.1727759333288163 0.774440428827406 6 0.12534724574835693 0.6742187262732712 7 0.09146961176231452 0.5739970237191363 8 0.04404092418185514 0.3158502141100009 9 0.030489870587438173 0.2459987244510584 >10 0.1456738261399824 2.0135451149512558 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 36 0.10933276642269261 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.04555531934278859 0.0 0.0 0.0 0.0 11 0.07896255352750023 0.0 0.0 0.0 0.0 12 0.08199957481701947 0.0 0.0 0.0 0.0 13 0.09111063868557719 0.0 0.0 0.0 0.0 14 0.10325872384365414 0.0 0.0 0.0 0.0 15 0.11236978771221186 0.0 0.0 0.0 0.0 16 0.1154068090017311 0.0 0.0 0.0 0.0 17 0.12148085158076959 0.0 0.0 0.0 0.0 18 0.12451787287028881 0.0 0.0 0.0 0.0 19 0.13970297931788503 0.0 0.0 0.0 0.0 20 0.15185106447596197 0.0 0.0 0.0 0.0 21 0.1700731922130774 0.0 0.0 0.0 0.0 22 0.1761472347921159 0.0 0.0 0.0 0.0 23 0.18222127737115437 0.0 0.0 0.0 0.0 24 0.2004434051082698 0.0 0.0 0.0 0.0 25 0.21259149026634677 0.0 0.0 0.0 0.0 26 0.21866553284538526 0.0 0.0 0.0 0.0 27 0.22777659671394296 0.0 0.0 0.0 0.0 28 0.22777659671394296 0.0 0.0 0.0 0.0 29 0.22777659671394296 0.0 0.0 0.0 0.0 30 0.23385063929298144 0.0 0.0 0.0 0.0 31 0.2368876605825007 0.0 0.0 0.0 0.0 32 0.2368876605825007 0.0 0.0 0.0 0.0 33 0.2368876605825007 0.0 0.0 0.0 0.0 34 0.2368876605825007 0.0 0.0 0.0 0.0 35 0.2368876605825007 0.0 0.0 0.0 0.0 36 0.23992468187201993 0.0 0.0 0.0 0.0 37 0.23992468187201993 0.0 0.0 0.0 0.0 38 0.23992468187201993 0.0 0.0 0.0 0.0 39 0.23992468187201993 0.0 0.0 0.0 0.0 40 0.23992468187201993 0.0 0.0 0.0 0.0 41 0.23992468187201993 0.0 0.0 0.0 0.0 42 0.24296170316153917 0.0 0.0 0.0 0.0 43 0.2520727670300969 0.0 0.0 0.0 0.0 44 0.2551097883196161 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGGC 15 0.008198346 50.24502 39 AAGACCT 15 0.008198346 50.24502 36 AGGCTAG 15 0.008198346 50.24502 37 GGCTAGG 15 0.008198346 50.24502 38 AGACCCT 15 0.008198346 50.24502 40 TGAAAAC 15 0.008198346 50.24502 34 CAAAACC 15 0.008198346 50.24502 34 TGCGCTT 20 4.0563062E-4 50.168194 49 CCCCGAC 15 0.008248195 50.168194 41 TATTTTA 15 0.008248195 50.168194 48 GTAGGGG 20 4.0563062E-4 50.168194 48 TAGGGGT 20 4.0563062E-4 50.168194 49 GTGCGCT 20 4.0563062E-4 50.168194 48 ATTTTAT 15 0.008248195 50.168194 49 TTTAAAT 15 0.008248195 50.168194 48 TAGGCTC 20 4.0563062E-4 50.168194 41 AGGGGTT 20 4.0870943E-4 50.091602 50 CAGTCAT 15 0.0082982695 50.091602 33 GTGCAAG 15 0.0082982695 50.091602 32 GTGTAGG 20 4.0870943E-4 50.091602 46 >>END_MODULE