FastQCFastQC Report
Fri 17 Jun 2016
SRR1526280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112626
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA3460.30721147869941223No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAACCAAAAAAAAAAAAAAA2920.2592651785555733No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAACCAAAAAAAAAAAAAAA2300.20421572283486938No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAAACCAAAACAAAAAAA1650.1465025837728411No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAACCAAAACAAAAAAA1590.14117521709019232No Hit
GAGATCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1580.1402873226430842No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACAAAAAAAAAAAAAAA1310.11631417257116473No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACAAAAAAAAAAAAAAA1300.1154262781240566No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAAACCAAAACAAAAAAA1130.10033207252321845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTG150.0079683550.68854541
CCACACG150.0079683550.68854540
ACTAACA150.00798262850.6656138
ACGGACC203.90413E-450.66560738
CGGCGAA251.9500514E-550.5284337
AGACCAT150.00806869550.5284337
CACGGAC203.9567592E-450.52842737
CCGGCGA309.817286E-750.41467736
AAGGCTA150.00814094350.41467736
ATACACA150.00814094350.41467736
AAAGGCT150.0081554550.3919935
TAAAGGC150.00824289750.25628334
ATAAAAC402.5484042E-950.2112148
GTTGGCT150.00827220150.2112149
ACAAGAG150.00828688150.18870542
CCCTGCC150.00830158150.16621843
CTTCACA150.00830158150.16621844
CACCATA301.0158001E-650.16621844
TGGATTA150.00836057650.07647750
CCAGGTG150.00837537350.05409233