##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526280_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 112626 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.244614920178286 39.0 38.0 39.0 33.0 40.0 2 35.97417115053362 38.0 35.0 39.0 29.0 40.0 3 36.255491627155365 38.0 36.0 39.0 29.0 40.0 4 34.93974748281924 38.0 35.0 39.0 20.0 40.0 5 36.1903912062934 39.0 36.0 39.0 28.0 40.0 6 36.66946353417506 38.0 37.0 39.0 31.0 40.0 7 36.52783549091684 38.0 36.0 39.0 31.0 40.0 8 36.399676806421255 38.0 36.0 39.0 30.0 40.0 9 36.35980146680163 38.0 36.0 39.0 30.0 40.0 10 36.085433203700745 38.0 35.0 39.0 29.0 40.0 11 35.074041517944345 38.0 34.0 39.0 28.0 40.0 12 32.98858167741019 37.0 31.0 39.0 18.0 40.0 13 32.280690071564294 36.0 29.0 39.0 16.0 40.0 14 32.27863903539147 36.0 29.0 39.0 15.0 40.0 15 32.15578995968959 36.0 29.0 39.0 16.0 40.0 16 32.337364374123204 36.0 29.0 39.0 18.0 40.0 17 32.30070321240211 36.0 29.0 38.0 17.0 40.0 18 32.523040860902455 36.0 30.0 38.0 19.0 40.0 19 33.36634524887681 36.0 31.0 38.0 23.0 40.0 20 33.53555129366221 36.0 31.0 39.0 23.0 40.0 21 34.75716086871593 38.0 34.0 39.0 26.0 40.0 22 34.446149201782895 38.0 33.0 39.0 24.0 40.0 23 34.82147106351997 38.0 34.0 39.0 26.0 40.0 24 34.27372010015449 38.0 33.0 39.0 24.0 40.0 25 34.63947933869622 38.0 33.0 39.0 26.0 40.0 26 34.9887947720775 38.0 35.0 39.0 27.0 40.0 27 34.91533038552377 38.0 35.0 39.0 27.0 40.0 28 35.09418784294923 38.0 35.0 39.0 28.0 40.0 29 34.955294514588104 38.0 35.0 39.0 27.0 40.0 30 34.729138919965195 38.0 35.0 39.0 26.0 40.0 31 34.48409781045229 38.0 34.0 39.0 25.0 40.0 32 33.35625876795766 38.0 33.0 39.0 18.0 40.0 33 34.61010779038588 38.0 35.0 39.0 26.0 40.0 34 34.435965052474565 38.0 35.0 39.0 25.0 40.0 35 34.296068403388205 38.0 34.0 39.0 26.0 40.0 36 31.88159039653366 35.0 31.0 38.0 19.0 39.0 37 31.501225294337008 35.0 30.0 38.0 16.0 39.0 38 30.854145579173547 35.0 30.0 38.0 10.0 39.0 39 28.914815406744445 33.0 26.0 38.0 4.0 39.0 40 24.730364214302202 31.0 10.0 37.0 4.0 39.0 41 21.170919681068316 24.0 8.0 32.0 4.0 36.0 42 25.055404613499547 30.0 19.0 33.0 4.0 37.0 43 21.063342389856693 25.0 7.0 32.0 4.0 36.0 44 22.21104363113313 27.0 8.0 33.0 4.0 36.0 45 24.727736046738762 30.0 18.0 34.0 4.0 37.0 46 24.928586649619092 30.0 9.0 36.0 4.0 38.0 47 24.097916999627085 30.0 4.0 35.0 4.0 38.0 48 24.032266084207908 29.0 4.0 35.0 4.0 38.0 49 21.36795233782608 26.0 4.0 35.0 4.0 38.0 50 23.74790012963259 29.0 4.0 35.0 4.0 38.0 51 25.33798590023618 32.0 4.0 38.0 4.0 39.0 52 25.083248983360857 32.0 4.0 38.0 4.0 39.0 53 24.683483387494896 31.0 4.0 38.0 4.0 39.0 54 23.83526894322803 31.0 4.0 37.0 4.0 39.0 55 23.947276827730718 31.0 4.0 37.0 4.0 39.0 56 23.482162200557596 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9675671703924777 1 2 2.0453390079758904 1 3 1.9078034729616107 1 4 3.7061678488861887 1 5 1.774026044530828 1 6 1.417525383706142 1 7 1.6578979238116034 1 8 2.0313393334963123 1 9 2.0347113632806924 1 10 1.5229044923916462 1 11 -6.231732795285705 1 12 -9.275606221149896 1 13 -21.032257431783997 1 14 -19.577148028142386 1 15 -13.303849259893575 1 16 -22.03736234962552 1 17 -21.311649724617233 1 18 -20.522305181027583 1 19 -6.973292366289584 1 20 -12.10864332128098 1 21 -10.776231441141388 1 22 -5.050502212793294 1 23 -5.289633869313029 1 24 -5.733310021289398 1 25 -5.445746501591593 1 26 1.0608581798746215 1 27 1.1378549040140626 1 28 1.1960280543544926 1 29 0.746472712165442 1 30 1.245653770198821 1 31 0.5518277555111055 1 32 -11.83926645621139 1 33 0.14219585141199786 1 34 0.23551244886221667 1 35 0.7668660469859105 1 36 3.2067638719635028 1 37 3.0345727214841176 1 38 4.049482643857413 1 39 6.593321664302508 1 40 9.016708638066358 1 41 -5.962112155544359 1 42 -11.602672385546427 1 43 -13.244296788885297 1 44 -15.481519993294604 1 45 -15.262182966805337 1 46 -18.14216910478364 1 47 -17.366397637062743 1 48 -16.236023509868694 1 49 -14.522760702374693 1 50 -15.971181570439754 1 51 -17.237507134875848 1 52 -16.969248769284697 1 53 -16.78662550793572 1 54 -17.208395616980823 1 55 -16.048002497253773 1 56 -15.344756210320673 2 1 0.9302894686140917 2 2 1.6965587982175663 2 3 1.748774745191433 2 4 2.8458167316951304 2 5 1.0446598794601485 2 6 1.0176165829765438 2 7 1.3595623104965142 2 8 1.0698140256987756 2 9 1.148311454479959 2 10 0.8854215922548505 2 11 -5.040727323329481 2 12 -12.043333079335031 2 13 -13.57871206014697 2 14 -16.701065036806316 2 15 -9.619683733217787 2 16 -6.437157151267108 2 17 -4.878510189733511 2 18 -12.704646722295255 2 19 -4.549443757078457 2 20 -6.145066029899311 2 21 0.4796965114349945 2 22 0.9589597115113122 2 23 0.697313235110137 2 24 0.586742418291081 2 25 0.9317614783445691 2 26 0.6613483759530538 2 27 0.7264321953957307 2 28 1.0456632572728708 2 29 0.7051480427628007 2 30 0.21521601370087495 2 31 -4.628860798980458 2 32 -12.4550325300828 2 33 -5.072806428569766 2 34 -4.058947195460629 2 35 -4.211702124468719 2 36 2.4323110675859425 2 37 2.048993606117037 2 38 3.2924147004009647 2 39 5.161949115282901 2 40 8.057235313852953 2 41 3.61723120970872 2 42 -0.27469700934943475 2 43 -3.7704595795829725 2 44 -9.847514065342029 2 45 -2.6005322600109935 2 46 -13.165139921017111 2 47 -12.444772010067759 2 48 -11.037779095824007 2 49 -12.801260474376516 2 50 -10.474658542623978 2 51 -11.398416847598147 2 52 -11.126168513926741 2 53 -11.102687979435949 2 54 -14.694715726419947 2 55 -10.548686491781815 2 56 -9.814318453822725 3 1 1.4790679964186566 3 2 2.715563941258388 3 3 2.352840961842034 3 4 3.9657942308915253 3 5 2.516051714882849 3 6 2.2021231724976005 3 7 2.4057693178896358 3 8 2.6305260154126913 3 9 2.7905888572443445 3 10 2.3319018236916875 3 11 0.6400425255936923 3 12 0.8671056863351723 3 13 -5.518723310548772 3 14 -4.2969345321454355 3 15 -2.4681649289747796 3 16 2.7689662817126433 3 17 2.8421182255670345 3 18 2.6782285589647934 3 19 1.2809966157920059 3 20 0.8909754358673254 3 21 3.042296961451065 3 22 3.8460538934463813 3 23 3.2850261891442045 3 24 3.562915674478802 3 25 3.2727691929058054 3 26 2.2903663765959337 3 27 2.088293333293514 3 28 1.9374183199536787 3 29 2.560299119586972 3 30 1.9025352036719454 3 31 2.622103521173834 3 32 1.276123046902093 3 33 2.4037284476758174 3 34 2.826057626140141 3 35 2.409641494946264 3 36 5.203968091051067 3 37 5.420136325499875 3 38 5.997252373174991 3 39 8.391135871952784 3 40 10.679181633364362 3 41 9.40845589630467 3 42 7.222972550277692 3 43 1.8039939723295966 3 44 1.9565280433047079 3 45 5.940129906494953 3 46 -0.6110978123703745 3 47 -3.9484283906527793 3 48 -0.008929851208129946 3 49 -9.638531448339872 3 50 -1.7787810112835736 3 51 -3.082722537087058 3 52 -2.4418628244378304 3 53 -1.9069711681212596 3 54 -6.447689046895677 3 55 -2.7248356399656792 3 56 -0.29671961100691036 4 1 1.8732184536796197 4 2 2.687986716535157 4 3 2.727058804929321 4 4 4.051470495791442 4 5 2.3288720099081885 4 6 2.3406888170515217 4 7 2.370915556458108 4 8 2.781068216494262 4 9 3.0434272728280263 4 10 2.7711271698226625 4 11 3.5710145236683886 4 12 2.812998831667713 4 13 5.083700314129164 4 14 2.4838633386889626 4 15 5.634697552975446 4 16 5.744418587836826 4 17 5.863070121147317 4 18 5.489463305135406 4 19 4.351043899123923 4 20 4.436121108168351 4 21 3.2242103791582437 4 22 3.8054242040440727 4 23 3.302806987015039 4 24 3.760203253402331 4 25 3.3298489516758707 4 26 2.3544678517226316 4 27 2.863966483722457 4 28 2.4671048909211493 4 29 2.7903735837563985 4 30 2.5388216217472035 4 31 3.0472288078467287 4 32 3.5377669213039766 4 33 2.6963188381172216 4 34 3.022354237029468 4 35 2.3631226240835517 4 36 4.93227296944513 4 37 5.346578183989642 4 38 5.6856637098492975 4 39 8.201266396599571 4 40 10.418443786879468 4 41 6.8066551658196595 4 42 9.334187477087625 4 43 8.534592233888532 4 44 11.872578894121546 4 45 9.298888648248685 4 46 8.294590344145949 4 47 8.554275536411975 4 48 8.096707341237863 4 49 4.413536843514372 4 50 8.289171082490316 4 51 6.572590467244886 4 52 6.633394119416753 4 53 6.35178474229523 4 54 2.3852903692825826 4 55 6.608582633484872 4 56 6.833578528804523 5 1 2.3447517032120686 5 2 3.237761373922403 5 3 3.2492098304681534 5 4 4.762541409732023 5 5 3.1123591485168873 5 6 2.733244846116122 5 7 3.1313165743322244 5 8 3.3046295495414455 5 9 3.179246017654343 5 10 2.6201936075698526 5 11 3.925553567725167 5 12 6.392665876460306 5 13 6.752431343960236 5 14 5.973056090934719 5 15 7.1626431028420825 5 16 6.869752002927115 5 17 6.986134780680693 5 18 6.1658283908269205 5 19 5.152435811316948 5 20 5.275008804768284 5 21 3.15973479893934 5 22 3.6447411246682933 5 23 3.477899730063143 5 24 3.776483107466163 5 25 3.3164203714876237 5 26 2.5795958524584464 5 27 2.343992237163917 5 28 2.7023809739453384 5 29 2.9013279940332453 5 30 2.8402595222285427 5 31 3.167258240351252 5 32 3.7974474559944724 5 33 3.2862259417748234 5 34 3.206889908611771 5 35 3.185791784337411 5 36 4.6158735458316755 5 37 4.996913898494348 5 38 5.359330815959613 5 39 7.559791092310398 5 40 5.225047705081636 5 41 3.051098247648156 5 42 8.627408803389862 5 43 12.719925035801644 5 44 11.771220706673287 5 45 8.258848178554967 5 46 9.179926211944949 5 47 9.744881662051977 5 48 9.963004057674077 5 49 10.971712028233995 5 50 9.60961747547557 5 51 9.266001878472164 5 52 9.505270748168627 5 53 9.431988317118176 5 54 9.149523744516358 5 55 9.598894434079654 5 56 9.36833034534191 6 1 1.8742670596505064 6 2 2.703561599338016 6 3 2.580007238188635 6 4 4.121727095840782 6 5 2.734435789824296 6 6 2.3204877314359322 6 7 2.778329817499312 6 8 2.784800020096533 6 9 2.878703611342708 6 10 2.244665289733838 6 11 3.69946875510189 6 12 6.379986248396058 6 13 6.428290740973477 6 14 6.1998453273393395 6 15 6.88136668695028 6 16 6.713052932766509 6 17 6.30006123883792 6 18 5.8338686838672125 6 19 5.059099660288489 6 20 4.8801440541343055 6 21 2.8210830660568433 6 22 3.4309485070295196 6 23 2.8139098737655956 6 24 3.1931849953219 6 25 2.549408537168098 6 26 1.691810560827001 6 27 1.900451803238866 6 28 2.137750092006762 6 29 1.8364505637712014 6 30 2.1721487868101192 6 31 2.94011679205596 6 32 3.417516489525042 6 33 2.5304848879568453 6 34 3.0502965020183623 6 35 2.576113001581703 6 36 3.249537958342245 6 37 3.0111784800666186 6 38 4.078829266227292 6 39 5.664750235731084 6 40 0.8874173866820847 6 41 1.3234637027059186 6 42 6.233706351995103 6 43 10.949778515655122 6 44 9.193452321212593 6 45 6.021316479484806 6 46 9.00082646553751 6 47 9.194233592173138 6 48 8.776296534428095 6 49 9.947338965399393 6 50 8.764837255695351 6 51 7.87434225604331 6 52 8.482024763384185 6 53 8.203684569583661 6 54 8.13476606629714 6 55 7.760137037629949 6 56 7.764463058782319 7 1 1.7662887806195897 7 2 2.5388790596554784 7 3 2.6284617076957346 7 4 4.156188248722991 7 5 2.433892765597065 7 6 2.199247129932175 7 7 2.5968344123196943 7 8 2.9225402038893478 7 9 2.6235866522783766 7 10 2.2762024660078595 7 11 3.2879190474995497 7 12 5.382492514060225 7 13 5.927350891349416 7 14 6.29936495975371 7 15 6.574975709506667 7 16 6.060692865932758 7 17 5.761109693307425 7 18 5.373655651285755 7 19 4.704254213337784 7 20 4.676614396657278 7 21 2.60293352553888 7 22 3.129152349970205 7 23 2.9112365237238222 7 24 2.9109168248958284 7 25 1.7299640927236553 7 26 1.8854195833833884 7 27 1.958200341250567 7 28 1.5018896074620685 7 29 2.1457655178230013 7 30 2.176430323986395 7 31 2.268542021796975 7 32 3.6550895889402497 7 33 2.2975066089259357 7 34 2.2806640876491073 7 35 1.8515933691673325 7 36 -0.5590460015575047 7 37 -0.12468200447807476 7 38 -0.12459596351372682 7 39 2.464666693542277 7 40 -4.497503248238548 7 41 -10.185830365798676 7 42 3.003338766364358 7 43 7.285826970124631 7 44 5.9123328558826 7 45 3.012126838716217 7 46 4.970437994359564 7 47 5.63972231397765 7 48 5.891271471771454 7 49 8.013755005499643 7 50 5.602869837148983 7 51 6.6203740020750566 7 52 7.159134203651519 7 53 6.898129014028104 7 54 7.330568071309671 7 55 7.0539966867527575 7 56 5.812186534137371 8 1 2.054883477996981 8 2 2.4557161421367297 8 3 3.2703373579666106 8 4 4.357996450208297 8 5 2.836612243227748 8 6 2.634722320977268 8 7 2.9791039921998674 8 8 3.1206530729741218 8 9 3.161167824021639 8 10 2.4022025377618874 8 11 3.6685382847542414 8 12 6.234499832970997 8 13 6.287807246962377 8 14 6.3080471970442815 8 15 6.637939868370083 8 16 6.722803151872554 8 17 6.939741344008823 8 18 6.076163454536218 8 19 4.9310686933004675 8 20 5.195108997166727 8 21 3.525618561236506 8 22 3.7766248143622647 8 23 3.328809084983824 8 24 3.7337561317519317 8 25 3.0946539359411034 8 26 2.5005309815106145 8 27 2.83424525927159 8 28 2.112516378889616 8 29 2.978650388486365 8 30 2.5368508025857537 8 31 3.277941799359489 8 32 4.343641233901323 8 33 2.888759793010891 8 34 2.563661970905308 8 35 2.844956121651819 8 36 -0.042201743673530245 8 37 -0.02530267681331111 8 38 0.9682537476763144 8 39 2.5524218688014884 8 40 -3.476371687229687 8 41 -9.755377956657213 8 42 3.6344513125560134 8 43 7.760982138208437 8 44 6.609315599039061 8 45 4.121228837065875 8 46 7.575432074652323 8 47 7.842714456911672 8 48 7.569131766680503 8 49 10.109331369723087 8 50 7.544964337202799 8 51 8.107763231795573 8 52 8.944338523243957 8 53 8.08763139984951 8 54 8.592003869393839 8 55 8.172713724746515 8 56 8.038893383059268 9 1 1.6486718215552685 9 2 2.964573504099924 9 3 3.0127453334267287 9 4 4.744346143459836 9 5 2.847233408871915 9 6 2.388940112388312 9 7 2.589341722261217 9 8 2.7297404962870075 9 9 3.0016202780093693 9 10 2.2089510040195535 9 11 3.8265520884352284 9 12 6.266593391253203 9 13 6.714302645735383 9 14 6.734964374958388 9 15 6.992080972664567 9 16 6.46394578990936 9 17 6.31523981026649 9 18 6.2413091600576855 9 19 4.9954091840980155 9 20 4.9997869112771625 9 21 3.296975923199696 9 22 4.286900887981901 9 23 3.290695588051314 9 24 3.845565947702852 9 25 3.1681585371680967 9 26 2.8462748465412844 9 27 3.3212851365722003 9 28 3.01661913962581 9 29 2.756986278056914 9 30 2.876613072524407 9 31 2.8457715539607165 9 32 3.400849822858376 9 33 2.763222983194943 9 34 3.127082216304075 9 35 2.399327287295989 9 36 1.0352522440565295 9 37 1.3004641943523332 9 38 2.208591170989198 9 39 3.9704049976358426 9 40 -2.7468088037941065 9 41 -3.450048202055985 9 42 5.219420637709387 9 43 9.248290420417028 9 44 8.148809464069735 9 45 5.1954236223419485 9 46 8.243683608394655 9 47 8.810007401696947 9 48 8.467367962999521 9 49 11.341386584447012 9 50 9.038944398552495 9 51 8.733568446519499 9 52 9.629346191955612 9 53 9.437017902916992 9 54 9.78506368534476 9 55 8.816387037629948 9 56 7.693034487353746 10 1 1.8696155301645376 10 2 2.2747142325767413 10 3 2.575907585082362 10 4 4.147822964651887 10 5 2.259112547944767 10 6 2.0367050130505646 10 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41 8.362667315185394 114 42 4.907351672192146 114 43 1.9206323744400144 114 44 -1.2901273175394614 114 45 2.3710701740660873 114 46 -3.1566037479271856 114 47 -7.1255529431306375 114 48 -3.451741232402778 114 49 -12.795682381070229 114 50 -3.1454665209877355 114 51 -4.440928679917281 114 52 -4.543426796550136 114 53 -4.914131522370361 114 54 -5.865439188218458 114 55 -4.179518134783844 114 56 -1.1244942482784381 115 1 1.1335402426079 115 2 2.7412182409420254 115 3 2.306824280795148 115 4 3.7877671960914157 115 5 1.908746566766652 115 6 1.8659137965988393 115 7 2.0225654064717418 115 8 2.330727338392272 115 9 2.386159751693583 115 10 1.7089510040195535 115 11 -0.7132505431437224 115 12 -4.013998714009954 115 13 -1.749842091106725 115 14 -4.517009309252135 115 15 -3.835550606282805 115 16 -10.489014736406428 115 17 -10.385089137101932 115 18 -3.338625050468629 115 19 -4.251301342217776 115 20 -3.8298183518807356 115 21 -0.2309846031160916 115 22 0.2987429932450567 115 23 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2.0228382475815962 116 6 1.592569144646376 116 7 2.110511077099929 116 8 2.2273883457493753 116 9 2.175746622095396 116 10 1.2358327244496579 116 11 -1.6298323201669263 116 12 -2.6276942431554 116 13 -7.093022623081822 116 14 -12.084524872353438 116 15 -6.152811500453716 116 16 -12.578997758477733 116 17 -9.624477931668995 116 18 -4.935908581877797 116 19 -5.349886514826718 116 20 -5.9468528736690764 116 21 0.7844087188986251 116 22 0.9597503503474911 116 23 0.2109912869760393 116 24 0.1791680982404813 116 25 0.3679569242648739 116 26 1.1468796852509655 116 27 1.4609356742066097 116 28 0.9797239783354925 116 29 0.8760050952612204 116 30 1.6957662983308524 116 31 -0.425666618082289 116 32 -9.062322220152375 116 33 0.16470147781859623 116 34 -0.2703640202550588 116 35 -0.3774200245319719 116 36 2.719392029002769 116 37 3.062829785750182 116 38 3.3330535365805964 116 39 5.733106610539068 116 40 8.42005947577579 116 41 3.6162824431053053 116 42 0.8231840785696036 116 43 -10.261050977432435 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0.7453819893984317 117 27 0.9837851365722017 117 28 0.8282262824829587 117 29 0.22930770662834732 117 30 0.7792916439529733 117 31 -0.4229784460392807 117 32 -9.17772160571305 117 33 0.41143726890922494 117 34 0.6038679305897929 117 35 0.5582558587245572 117 36 2.0531093869136754 117 37 2.0290356229237645 117 38 3.1085911709891967 117 39 3.962369283350128 117 40 3.946941196205895 117 41 3.204416083658298 117 42 0.858706351995103 117 43 -6.3954595795829725 117 44 -2.4672619645016916 117 45 2.253459336627664 117 46 -1.7134592487482045 117 47 -6.585528312588766 117 48 -5.045132037000478 117 49 -6.415756272695845 117 50 -1.2173056014475065 117 51 -2.1137529820519276 117 52 -4.285832379472961 117 53 -5.4986963827972914 117 54 -9.12118631465524 117 55 -6.400577248084337 117 56 -3.5212512269319696 118 1 0.9644850745673139 118 2 2.2363355522926938 118 3 2.084883887643599 118 4 3.395249757917668 118 5 2.0613147341731235 118 6 1.2692111967256636 118 7 1.8186338909359137 118 8 1.985388086648456 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0.8461908235114635 119 30 0.24952973919106824 119 31 -1.2487360217968586 119 32 -9.252938055929501 119 33 -0.203822471350513 119 34 -0.500569298847438 119 35 -0.44877877331007454 119 36 2.539040122844405 119 37 2.100464194352334 119 38 3.2411669285649545 119 39 4.411503482484328 119 40 6.2651230143877115 119 41 5.0815805858228025 119 42 0.8603297286184777 119 43 -1.6075807917041836 119 44 -2.9212662935060223 119 45 -2.1691597109913836 119 46 -7.9131345734235286 119 47 -7.837151689212144 119 48 -10.70043506730351 119 49 -5.721492203431774 119 50 -8.164275298417204 119 51 -10.389727008025956 119 52 -10.150009868650448 119 53 -12.364497248598159 119 54 -11.022701466170393 119 55 -12.323196295703385 119 56 -9.69711702779777 120 1 0.7611718215552656 120 2 1.8015053222817414 120 3 1.80024533342673 120 4 3.46480068891438 120 5 1.512574317962823 120 6 0.26962193057013195 120 7 0.921728085897584 120 8 0.9655359508324608 120 9 0.909006641645739 120 10 0.74304191311046 120 11 -7.035379729746591 120 12 -2.2498838814740694 120 13 -4.541947354264618 120 14 -3.059353806859793 120 15 -9.037464481880889 120 16 -8.032076937363364 120 17 -8.458055644278964 120 18 -3.849031749033223 120 19 -2.4057271795383492 120 20 -4.717258543268294 120 21 -1.5035922586184824 120 22 -1.3699172938362807 120 23 -2.2331680483123257 120 24 -6.081706779569878 120 25 -1.9733187355591753 120 26 -1.4656569716405343 120 27 -1.0162148634277983 120 28 -1.3373581331014606 120 29 -0.7788091764885365 120 30 -2.451227836566506 120 31 -7.2184329914938274 120 32 -10.896877449868896 120 33 -6.65836792589597 120 34 -12.394508692786832 120 35 -5.194990894522196 120 36 -3.703384119579834 120 37 -3.7290812601931194 120 38 -4.414136101738077 120 39 -1.68698136600052 120 40 -4.189422440157742 120 41 -1.38811638387417 120 42 -5.480579362290612 120 43 -3.3159141250375175 120 44 -4.595508717748446 120 45 -11.335826377658051 120 46 -11.515407300696255 120 47 -11.291697143757597 120 48 -9.374677491545933 120 49 -14.04724977918935 120 50 -8.933214692356598 120 51 -9.276090644389592 120 52 -8.793949262589843 120 53 -9.599345733446643 120 54 -9.814368132837059 120 55 -9.967135689642777 120 56 -5.852420058100799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1151.0 5 71.0 6 71.0 7 117.0 8 283.0 9 309.0 10 228.0 11 197.0 12 234.0 13 258.0 14 322.0 15 511.0 16 633.0 17 873.0 18 1061.0 19 1209.0 20 1559.0 21 1963.0 22 2422.0 23 2818.0 24 3199.0 25 3473.0 26 3704.0 27 3924.0 28 4189.0 29 4176.0 30 4761.0 31 5611.0 32 6413.0 33 7698.0 34 9225.0 35 10846.0 36 12281.0 37 10039.0 38 5159.0 39 1638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.39711967041359 0.46348090139044273 0.0861257613694884 0.053273666826487674 8 99.01443716370999 0.7928897412675582 0.12608101148935416 0.06659208353310958 9 93.04676051334428 4.680492028953329 1.2878014121408587 0.9849460455615258 10 42.26111199900556 38.538170582281175 8.684495587164598 10.516221831548664 11 25.75540973708278 37.994690155477215 18.732740785466298 17.5171593219737 12 32.131124251948926 15.135048745405147 20.033562410100686 32.70026459254524 13 25.582902704526482 20.15165237156607 21.46396036439188 32.801484559515565 14 21.058100499120766 19.01277109717757 19.92131298957353 40.00781541412814 15 33.9467084081894 16.585140255073906 21.591454846226366 27.876696490510326 16 23.577372969420292 19.835510831238732 22.98229876277855 33.60481743656242 17 28.220384892054383 28.545420637105583 20.51011074305303 22.724083727787004 18 34.478533055099234 23.09017448830085 20.145628912667053 22.28566354393287 19 33.95257969984904 25.247313737678713 18.999200781458132 21.80090578101412 20 33.961861960547466 23.523194571502163 21.435993995967706 21.078949471982664 21 26.681407285062246 30.458912746194965 23.228017830820324 19.63166213792246 22 25.778239482890275 22.66439365688207 27.68632464972564 23.871042210502015 23 32.775735620549426 25.70010477154476 22.121890149699002 19.40226945820681 24 25.426633281835453 31.641006517145243 22.648411556834127 20.28394864418518 25 29.82641509433962 23.313651498335183 25.398446170921197 21.461487236403993 26 22.013786969090503 30.104514120524794 26.863242161440958 21.01845674894374 27 21.483001172332944 18.215567160467515 37.654090731464706 22.64734093573484 28 30.58984666832687 19.890426553772812 31.015866804226405 18.50385997367391 29 31.212640666494952 19.452166730320013 30.45679418060379 18.878398422581245 30 24.000177627780985 24.047248989741995 33.57609130067943 18.376482081797594 31 23.0855485053707 23.157632443111535 33.78244889604784 19.974370155469927 32 17.448097039444473 26.997531390413272 35.43342746017369 20.120944109968566 33 16.425731035771157 29.375761727263832 38.38927488012526 15.809232356839756 34 16.900857663422656 29.793360885215304 39.63738168244234 13.6683997689197 35 17.781884083721263 31.791691837532426 37.548416900607656 12.87800717813866 36 16.656587933978372 32.07260244735345 36.79923164484917 14.471577973819011 37 15.594541910331383 32.56250723205782 38.50036049026676 13.342590367344032 38 18.149402904117874 31.84126052587119 38.800117374022996 11.209219195987941 39 16.49509345169657 26.112106384306177 34.934089146768635 22.458711017228623 40 11.21244060166494 19.637178891707457 27.981528457608345 41.16885204901926 41 12.881474317644532 22.613546815674475 30.057668887456124 34.44730997922487 42 13.963855743306372 33.99357554078464 40.26142744009325 11.781141275815738 43 10.231290578270976 18.00361586348779 30.181772841837144 41.58332071640409 44 11.055693356412498 20.90954513820732 28.057245846642516 39.977515658737666 45 14.3186040516636 32.429309226023804 40.02151168475604 13.230575037556559 46 14.685656407704883 30.97235055796432 40.825573956926576 13.51641907740422 47 14.068745003996803 28.336441957545077 37.80975219824141 19.785060840216715 48 15.603265262615585 31.743930928503538 40.35566135780208 12.297142451078798 49 11.43559462420178 20.552007908728992 27.65116092660379 40.36123654046544 50 14.863229457723042 32.705689725747035 39.79960490487462 12.631475911655308 51 14.911170904684687 33.36926887506905 38.80593025535024 12.913629964896026 52 13.878810031113211 35.27560599441924 37.91621720409019 12.92936677037737 53 12.96699972418211 35.99156531100691 37.90271636133923 13.138718603471746 54 11.994737356766318 31.518965961721385 34.062280537998596 22.424016143513704 55 14.183829545050333 34.96158360900651 37.423078291182016 13.431508554761143 56 13.460118661104243 36.01907116997714 36.668416043265935 13.852394125652681 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 2.0 8 5.5 9 9.0 10 17.5 11 26.0 12 103.5 13 262.0 14 343.0 15 391.0 16 439.0 17 506.5 18 574.0 19 741.5 20 909.0 21 1094.5 22 1481.5 23 1683.0 24 2080.0 25 2477.0 26 3012.5 27 3548.0 28 4069.0 29 4883.5 30 5177.0 31 5341.0 32 5505.0 33 5466.0 34 5427.0 35 5247.5 36 5068.0 37 5246.5 38 5239.0 39 5053.0 40 4584.5 41 4116.0 42 3609.5 43 3103.0 44 2961.0 45 2819.0 46 2755.5 47 2603.5 48 2515.0 49 2561.0 50 2607.0 51 2724.5 52 2842.0 53 3136.0 54 3772.5 55 4115.0 56 4561.5 57 5008.0 58 5293.0 59 5578.0 60 5518.0 61 5458.0 62 5115.0 63 4404.0 64 4036.0 65 3807.0 66 3578.0 67 3114.5 68 2651.0 69 2291.5 70 1932.0 71 1609.5 72 1094.0 73 901.0 74 798.0 75 695.0 76 538.5 77 382.0 78 308.5 79 163.0 80 91.0 81 68.5 82 46.0 83 29.0 84 12.0 85 9.5 86 7.0 87 3.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02752472786035196 10 0.0 11 0.004439472235540639 12 0.0 13 0.0 14 0.024861044519027577 15 0.16692415605632802 16 0.03107630564878447 17 0.02042157228348694 18 0.009766838918189406 19 0.014206311153730045 20 0.03107630564878447 21 0.010654733365297533 22 0.0 23 0.0 24 0.0 25 8.878944471081278E-4 26 0.17846678386873369 27 0.026636833413243837 28 0.16869994495054427 29 0.032852094543000726 30 0.02752472786035196 31 0.22818887290678883 32 0.010654733365297533 33 0.19267309502246371 34 0.09855628362900218 35 0.05504945572070392 36 0.15804521158524673 37 0.24772255074316765 38 0.1465025837728411 39 0.38090671780938684 40 0.5948892795624456 41 0.847051302541154 42 0.21575835064727508 43 0.30454779535808785 44 0.486566157015254 45 0.11453838367694849 46 0.06570418908600145 47 0.0319642000958926 48 0.041731039014082004 49 0.3063235842523041 50 0.22019782288281567 51 0.3445030454779536 52 0.4039919734341982 53 0.20687940617619377 54 0.11986575035959725 55 0.15449363379681424 56 0.1820183616571662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 112626.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.120664855362 #Duplication Level Percentage of deduplicated Percentage of total 1 93.88116994200358 79.91227602862571 2 3.9189301956857348 6.671638875570472 3 0.9659114615930238 2.466570774066379 4 0.3901197479868152 1.3282900928737593 5 0.22009429632411232 0.9367286416990748 6 0.12621521258396962 0.6446113686005008 7 0.08553427629657445 0.5096514126400654 8 0.06884466140943797 0.4688082680730915 9 0.04589644093962532 0.3516062010548186 >10 0.27016314098552174 4.118054445687497 >50 0.016689614887136477 0.9695807362420755 >100 0.0104310093044603 1.6221831548665495 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA 346 0.30721147869941223 No Hit GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAACCAAAAAAAAAAAAAAA 292 0.2592651785555733 No Hit GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAACCAAAAAAAAAAAAAAA 230 0.20421572283486938 No Hit GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAAACCAAAACAAAAAAA 165 0.1465025837728411 No Hit GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAACCAAAACAAAAAAA 159 0.14117521709019232 No Hit GAGATCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 158 0.1402873226430842 No Hit GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACAAAAAAAAAAAAAAA 131 0.11631417257116473 No Hit GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACAAAAAAAAAAAAAAA 130 0.1154262781240566 No Hit GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAAAACCAAAACAAAAAAA 113 0.10033207252321845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.6268534796583383 0.0 0.0 0.0 0.0 3 0.6268534796583383 0.0 0.0 0.0 0.0 4 0.6268534796583383 0.0 0.0 0.0 0.0 5 0.6268534796583383 0.0 0.0 0.0 0.0 6 0.6268534796583383 0.0 0.0 0.0 0.0 7 0.6268534796583383 0.0 0.0 0.0 0.0 8 0.6268534796583383 0.0 0.0 0.0 0.0 9 0.6277413741054464 0.0 0.0 0.0 0.0 10 0.6339566352352033 0.0 0.0 0.0 0.0 11 0.639284001917852 0.0 0.0 0.0 0.0 12 0.6401718963649601 0.0 0.0 0.0 0.0 13 0.6437234741533926 0.0 0.0 0.0 0.0 14 0.6561539964129064 0.0 0.0 0.0 0.0 15 0.6614813630955552 0.0 0.0 0.0 0.0 16 0.6632571519897714 0.0 0.0 0.0 0.0 17 0.6659208353310958 0.0 0.0 0.0 0.0 18 0.673911885355069 0.0 0.0 0.0 0.0 19 0.6801271464848259 0.0 0.0 0.0 0.0 20 0.6872303020616909 0.0 0.0 0.0 0.0 21 0.7032124021096372 0.0 0.0 0.0 0.0 22 0.7200823966046916 0.0 0.0 0.0 0.0 23 0.7378402855468542 0.0 0.0 0.0 0.0 24 0.7591497522774493 0.0 0.0 0.0 0.0 25 0.7715802745369631 0.0 0.0 0.0 0.0 26 0.7795713245609361 0.0 0.0 0.0 0.0 27 0.7840107967964768 0.0 0.0 0.0 0.0 28 0.799105002397315 0.0 0.0 0.0 0.0 29 0.799105002397315 0.0 0.0 0.0 0.0 30 0.7999928968444231 0.0 0.0 0.0 0.0 31 0.7999928968444231 0.0 0.0 0.0 0.0 32 0.8008807912915312 0.0 0.0 0.0 0.0 33 0.8008807912915312 0.0 0.0 0.0 0.0 34 0.8008807912915312 0.0 0.0 0.0 0.0 35 0.8008807912915312 0.0 0.0 0.0 0.0 36 0.8008807912915312 0.0 0.0 0.0 0.0 37 0.8008807912915312 0.0 0.0 0.0 0.0 38 0.8008807912915312 0.0 0.0 0.0 0.0 39 0.8008807912915312 0.0 0.0 0.0 0.0 40 0.8017686857386394 0.0 0.0 0.0 0.0 41 0.8035444746328556 0.0 0.0 0.0 0.0 42 0.8070960524212881 0.0 0.0 0.0 0.0 43 0.8150871024452613 0.0 0.0 0.0 0.0 44 0.8221902580221263 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCCTG 15 0.00796835 50.688545 41 CCACACG 15 0.00796835 50.688545 40 ACTAACA 15 0.007982628 50.66561 38 ACGGACC 20 3.90413E-4 50.665607 38 CGGCGAA 25 1.9500514E-5 50.52843 37 AGACCAT 15 0.008068695 50.52843 37 CACGGAC 20 3.9567592E-4 50.528427 37 CCGGCGA 30 9.817286E-7 50.414677 36 AAGGCTA 15 0.008140943 50.414677 36 ATACACA 15 0.008140943 50.414677 36 AAAGGCT 15 0.00815545 50.39199 35 TAAAGGC 15 0.008242897 50.256283 34 ATAAAAC 40 2.5484042E-9 50.21121 48 GTTGGCT 15 0.008272201 50.21121 49 ACAAGAG 15 0.008286881 50.188705 42 CCCTGCC 15 0.008301581 50.166218 43 CTTCACA 15 0.008301581 50.166218 44 CACCATA 30 1.0158001E-6 50.166218 44 TGGATTA 15 0.008360576 50.076477 50 CCAGGTG 15 0.008375373 50.054092 33 >>END_MODULE