FastQCFastQC Report
Fri 17 Jun 2016
SRR1526279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88412
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1290.145907795321902No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1180.13346604533321269No Hit
AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1020.11536895444057367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC150.00802200850.59344538
AAATAAT150.0081875150.3329535
TATGCCT150.0081875150.3329535
AAAGGCT204.0284457E-450.3329535
AAGGCTA204.0284457E-450.3329536
ATATGCT451.2732926E-1050.27543334
TCGACCC150.0082246350.2754334
CCAGGTC150.00828054550.1893943
CATTGAC150.00828054550.1893943
CGATTAA150.00828054550.1893949
CAAGGAT150.00828054550.1893949
ACCAGCG150.00828054550.1893942
CTGCAAT355.06152E-850.1893949
CACCGCG150.00828054550.1893944
CCTTGAC150.00828054550.1893943
CAACGAT150.008299245550.16077446
AAGAATG150.008299245550.16077448
ACGATTA150.008299245550.16077448
ACCGCGC150.00831797850.13219545
TCCACGA150.00831797850.13219545
Produced by FastQC (version 0.11.5)