##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526279_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88412 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64383794055106 38.0 36.0 39.0 33.0 40.0 2 36.37954123874587 38.0 36.0 39.0 31.0 40.0 3 35.3188707415283 38.0 35.0 39.0 26.0 40.0 4 34.523831606569246 38.0 34.0 39.0 23.0 40.0 5 35.65906211826449 38.0 35.0 39.0 29.0 40.0 6 35.60872958421934 38.0 36.0 39.0 28.0 40.0 7 35.7342442202416 38.0 36.0 39.0 30.0 40.0 8 35.67349454825137 38.0 35.0 39.0 29.0 40.0 9 35.721225625480706 38.0 36.0 39.0 29.0 40.0 10 35.28703117223906 38.0 35.0 39.0 28.0 40.0 11 34.99736461113876 38.0 34.0 39.0 28.0 40.0 12 32.66062299235398 37.0 30.0 39.0 17.0 40.0 13 32.11060715739945 36.0 29.0 39.0 14.0 40.0 14 31.82232049947971 36.0 29.0 39.0 13.0 40.0 15 32.302617291770346 37.0 30.0 39.0 16.0 40.0 16 32.182452608243224 36.0 30.0 39.0 16.0 40.0 17 32.3708433244356 36.0 30.0 39.0 17.0 40.0 18 32.48595213319459 36.0 30.0 39.0 19.0 40.0 19 33.31551146903136 36.0 31.0 39.0 23.0 40.0 20 33.38149798669864 36.0 31.0 39.0 23.0 40.0 21 34.86004162330905 38.0 35.0 39.0 26.0 40.0 22 34.586515405148624 38.0 34.0 39.0 25.0 40.0 23 34.72511650002262 38.0 34.0 39.0 26.0 40.0 24 34.39098764873547 38.0 33.0 39.0 24.0 40.0 25 34.62194046057096 38.0 34.0 39.0 26.0 40.0 26 35.023198208388 38.0 35.0 39.0 27.0 40.0 27 34.95519838935891 38.0 35.0 39.0 27.0 40.0 28 34.85429579695064 38.0 35.0 39.0 27.0 40.0 29 34.76279238112473 38.0 35.0 39.0 27.0 40.0 30 34.59109623128082 38.0 35.0 39.0 26.0 40.0 31 34.36844545989232 38.0 35.0 39.0 25.0 40.0 32 33.223419897751434 38.0 33.0 39.0 17.0 40.0 33 34.39193774600733 38.0 34.0 39.0 26.0 40.0 34 34.14321585305162 38.0 34.0 39.0 24.0 40.0 35 34.157523865538614 38.0 34.0 39.0 25.0 40.0 36 31.973804460932904 35.0 31.0 38.0 19.0 39.0 37 31.764522915441344 35.0 31.0 38.0 17.0 39.0 38 31.145953038049132 35.0 30.0 38.0 10.0 39.0 39 29.131079491471745 34.0 27.0 38.0 4.0 39.0 40 24.86447541057775 31.0 11.0 37.0 4.0 39.0 41 21.36490521648645 25.0 8.0 33.0 4.0 37.0 42 25.374451431932318 30.0 20.0 34.0 4.0 37.0 43 21.216316789576076 25.0 7.0 33.0 4.0 36.0 44 22.198977514364564 27.0 8.0 33.0 4.0 36.0 45 24.932633579152153 30.0 19.0 34.0 4.0 37.0 46 24.96930280957336 30.0 11.0 36.0 4.0 38.0 47 24.23459485137764 30.0 6.0 35.0 4.0 38.0 48 24.245011989322716 30.0 5.0 35.0 4.0 38.0 49 21.543116319051713 26.0 4.0 35.0 4.0 38.0 50 24.056564719721305 29.0 4.0 35.0 4.0 38.0 51 25.554155544496222 32.0 4.0 38.0 4.0 39.0 52 25.329604578563995 32.0 4.0 38.0 4.0 39.0 53 24.953558340496766 32.0 4.0 38.0 4.0 39.0 54 24.047855494729223 31.0 4.0 37.0 4.0 39.0 55 24.29199656155273 31.0 4.0 37.0 4.0 39.0 56 23.494966746595484 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.9224347246516444 1 2 2.2266845914901836 1 3 3.829589705110891 1 4 3.46267980666844 1 5 3.2706025627791533 1 6 3.2845987371243126 1 7 3.2710934214523135 1 8 3.220534000418347 1 9 3.069350622196758 1 10 3.29231328431613 1 11 -2.9513404232898353 1 12 -7.321197114828081 1 13 -20.221901400779096 1 14 -18.18860458854615 1 15 -11.839451434704422 1 16 -20.321252294832554 1 17 -20.76805033155488 1 18 -20.617898384617945 1 19 -5.487205846471031 1 20 -9.974497390065128 1 21 -7.749804100136451 1 22 -2.7821945546675195 1 23 -2.773720411944673 1 24 -2.906480795097657 1 25 -2.8870684830986377 1 26 1.7919691906792465 1 27 2.587998689962923 1 28 2.215085230026375 1 29 1.6826102723919192 1 30 2.250471720912053 1 31 1.93214088780109 1 32 -11.057637115802958 1 33 1.0934056722212446 1 34 1.0706825358366743 1 35 0.9153513834445874 1 36 3.5917374332728507 1 37 4.116547391234448 1 38 4.2208253050604085 1 39 5.820083550386613 1 40 9.064824837775902 1 41 -1.7001549397161284 1 42 -6.530721078312304 1 43 -12.305180659001069 1 44 -14.772119633685545 1 45 -13.612554547357774 1 46 -17.46229709762816 1 47 -17.18488926896358 1 48 -15.201975143979483 1 49 -14.142032457913022 1 50 -15.292541108656277 1 51 -16.927222384428827 1 52 -16.37417264451176 1 53 -16.24011936700026 1 54 -17.436441990045967 1 55 -16.161858681628086 1 56 -15.211750561394865 2 1 1.8576959512810305 2 2 2.3564242385284544 2 3 3.727982156622339 2 4 3.986618300465395 2 5 3.445248727378143 2 6 3.109952572525323 2 7 3.3291049538653894 2 8 3.260722712632223 2 9 3.205293309598474 2 10 3.342636541349158 2 11 -2.5149434287065766 2 12 -10.67791561910905 2 13 -11.604655201233403 2 14 -14.323498874759673 2 15 -6.912402684049173 2 16 -3.590604033431191 2 17 -2.511017306917452 2 18 -10.198101075514327 2 19 -2.228687586817003 2 20 -2.998960084456183 2 21 2.6487630849063564 2 22 3.0475276190176146 2 23 2.9629561562887687 2 24 2.761612862075154 2 25 2.813001410313909 2 26 2.267768596585242 2 27 2.4401741224284095 2 28 2.8355639999023197 2 29 2.5597725998425602 2 30 2.7281058288973767 2 31 -0.5277577667507174 2 32 -7.7281770424148775 2 33 -0.9334159248432314 2 34 -0.6251063860574391 2 35 0.00516441856602512 2 36 4.706886830442162 2 37 4.625808268396774 2 38 5.622625060433464 2 39 8.009494698385907 2 40 10.666519753030137 2 41 7.030056487856822 2 42 3.5169811757502494 2 43 -2.4253099618142784 2 44 -9.041733472581228 2 45 0.9579504325478716 2 46 -11.605229126284458 2 47 -11.56432313232196 2 48 -8.713332823518186 2 49 -11.660991046066089 2 50 -8.143056572573801 2 51 -8.098111778103473 2 52 -8.341060640318153 2 53 -7.565385835635592 2 54 -14.221869213530153 2 55 -8.618262665552603 2 56 -8.285488111630755 3 1 2.294378759997741 3 2 2.8342692748480616 3 3 4.011033004079962 3 4 4.221000867051352 3 5 4.0043286305258405 3 6 3.9651583836633364 3 7 3.4708725083448044 3 8 3.755880097620114 3 9 3.552266675215904 3 10 3.746510633358838 3 11 2.1436522129399123 3 12 2.795813194450268 3 13 -4.917851327141392 3 14 -5.0084867682221414 3 15 -0.3946060738796824 3 16 3.217628412089393 3 17 3.023849521169719 3 18 2.56001030463095 3 19 1.446372945870646 3 20 1.4213788985946678 3 21 3.4016686539136245 3 22 3.491958611753688 3 23 3.2204622095575317 3 24 3.976140707111476 3 25 3.396657584648054 3 26 2.696461090531976 3 27 2.4787939771499623 3 28 3.025394508376891 3 29 2.9447604933050258 3 30 1.8652728991152898 3 31 2.6789008288909244 3 32 1.5864718679967424 3 33 2.5403128887160875 3 34 3.170535260431663 3 35 2.2033484379364836 3 36 4.869719760224246 3 37 4.799537081956096 3 38 5.592842978109012 3 39 7.84629857247792 3 40 10.729110552061613 3 41 9.474366415217599 3 42 6.8944630159439555 3 43 1.3959975442389911 3 44 2.5795740334720385 3 45 6.705044863540607 3 46 -0.6749627824587918 3 47 -4.985993834501137 3 48 0.053253132898277045 3 49 -9.286656905630254 3 50 -0.8573422868595166 3 51 -1.8803151679339827 3 52 -1.9143051923762648 3 53 -2.2535214288559295 3 54 -6.883128293433302 3 55 -2.521652496061076 3 56 0.43581939442251283 4 1 2.7795291359375867 4 2 3.0910361921412957 4 3 4.447875109343123 4 4 4.807091092615266 4 5 4.19023088616494 4 6 4.114970413738526 4 7 3.9704965684951787 4 8 4.059451526191545 4 9 4.101702765441466 4 10 3.9444429641859102 4 11 3.7329379272256276 4 12 3.870813194450271 4 13 5.648690026242065 4 14 3.8168891716274835 4 15 6.898814978751901 4 16 6.985109615096906 4 17 6.93813523545543 4 18 6.282942635458021 4 19 5.407463171434561 4 20 5.260852582805192 4 21 3.736631059928662 4 22 4.547973649347675 4 23 3.7798607057981357 4 24 4.3917422108708735 4 25 3.683875629760834 4 26 2.908491165719944 4 27 3.0477789395559753 4 28 2.975394508376894 4 29 3.162805606086984 4 30 3.3509871848295774 4 31 3.2843519567104735 4 32 4.200569612357647 4 33 3.0626813097687204 4 34 3.361512704040692 4 35 2.77590482891393 4 36 4.728742316615222 4 37 4.983747608271884 4 38 5.79434673750751 4 39 8.065471504808748 4 40 10.55166694303906 4 41 5.92267468589429 4 42 8.467583316695833 4 43 7.730020100629968 4 44 11.784649221441963 4 45 8.955608773315042 4 46 9.410939473180306 4 47 9.121336992566533 4 48 8.960395990041135 4 49 6.1045085079035815 4 50 9.225364480057777 4 51 7.8117900952239125 4 52 7.600732401608699 4 53 7.52805751851249 4 54 1.5198792253636917 4 55 7.994136977623132 4 56 7.336947213971385 5 1 2.805324289288592 5 2 3.3174655749508375 5 3 4.743407104799388 5 4 4.992532213402846 5 5 4.2649658350479385 5 6 4.088796615934662 5 7 3.9019619225277467 5 8 4.029775267198737 5 9 3.9548360482888754 5 10 4.032430468918967 5 11 4.379520661038576 5 12 7.130705280781207 5 13 7.085077758161795 5 14 6.647114465076935 5 15 7.216545005257011 5 16 7.10632317056832 5 17 6.72590501962809 5 18 6.605642370406905 5 19 5.505674076394801 5 20 5.8328896899615685 5 21 4.48805097662688 5 22 4.238053164682775 5 23 3.9734426823221796 5 24 4.271001960965535 5 25 3.963409897083814 5 26 2.8228228582606505 5 27 3.3801814386093625 5 28 3.3261139328373233 5 29 2.6682959506534374 5 30 3.147749774757635 5 31 3.6620971289937003 5 32 4.96216559872645 5 33 3.2708884282844366 5 34 3.3929401936279646 5 35 3.362803730845016 5 36 5.024652956524353 5 37 4.945220535193506 5 38 5.658413378931215 5 39 7.738796003104845 5 40 7.777055053603238 5 41 4.201498994868164 5 42 9.499910086858648 5 43 13.267837215359236 5 44 13.252133129052716 5 45 9.845281245863323 5 46 10.518048522782728 5 47 10.991035970226513 5 48 10.311115414501565 5 49 10.28621051471918 5 50 9.817375082102458 5 51 9.23587188242573 5 52 8.69643478706875 5 53 9.8335289308563 5 54 9.417077256412536 5 55 9.39417484206842 5 56 8.317731007988804 6 1 2.7538662191035215 6 2 3.30357134682189 6 3 4.462941401026534 6 4 4.733868915033909 6 5 3.936062545465866 6 6 4.283260891842183 6 7 3.99982561630555 6 8 3.997428625428185 6 9 3.6714596959356456 6 10 4.11815730729559 6 11 4.754884492111124 6 12 7.490088003610573 6 13 7.331494365334073 6 14 7.939277681069026 6 15 7.701195452837872 6 16 7.51348446443399 6 17 7.590806991180624 6 18 6.690435604521902 6 19 5.318019619807398 6 20 6.137409432945809 6 21 4.035747170816563 6 22 4.868785220259689 6 23 4.212665044890144 6 24 4.219434054984973 6 25 4.190605240046089 6 26 3.788663925864583 6 27 3.7584559182622854 6 28 3.6147074854761314 6 29 4.098522761571111 6 30 3.9826665741425558 6 31 4.426828855063228 6 32 5.3497761209956565 6 33 4.242221285662659 6 34 4.821135042329161 6 35 3.7337192122003913 6 36 3.967593260769501 6 37 3.9999187613454126 6 38 5.33351909588437 6 39 7.140300753503006 6 40 3.278565295791168 6 41 1.8991210498958964 6 42 7.683885044297281 6 43 12.462191655471269 6 44 10.765615472948593 6 45 7.990813674881938 6 46 10.35906120881711 6 47 10.451138117516308 6 48 10.538640264850294 6 49 12.993168612363203 6 50 10.021065564830778 6 51 9.96205124427976 6 52 10.406578122781859 6 53 10.104493838319645 6 54 11.289063636773896 6 55 10.346286435236635 6 56 10.437891368250213 7 1 2.8960271116460916 7 2 3.1853681759469694 7 3 4.50334069638766 7 4 4.716605262655747 7 5 4.314218740415953 7 6 4.264059482564434 7 7 4.080213167685464 7 8 4.038297680037701 7 9 3.995123818073047 7 10 4.13442272127093 7 11 4.250245619533317 7 12 6.71504396368104 7 13 7.459621200331135 7 14 7.893711033975659 7 15 7.586163156889548 7 16 7.450595445056425 7 17 6.94582754314774 7 18 6.594076238696886 7 19 5.788131187628888 7 20 6.01368659090236 7 21 4.156064258309229 7 22 4.638661908456989 7 23 4.371561110656437 7 24 4.736580267551034 7 25 4.218086156076623 7 26 3.516790760861646 7 27 3.985936834292815 7 28 3.3100098929922765 7 29 3.7117934603379936 7 30 3.4548333386757335 7 31 3.9838458838359827 7 32 5.60350483502971 7 33 4.174928273331474 7 34 4.148557238453641 7 35 3.8159858005738485 7 36 2.7032362437407293 7 37 2.941844774263785 7 38 3.501634186900219 7 39 4.781463407642754 7 40 -0.7659443929933296 7 41 -9.035523694672513 7 42 6.1142432357241745 7 43 11.141052489293934 7 44 9.516936670834678 7 45 6.073726182221925 7 46 8.419542712046702 7 47 9.42994023143293 7 48 9.025780605425751 7 49 11.569387050413702 7 50 8.826174196656968 7 51 10.238915601296785 7 52 10.277453049381975 7 53 10.331093955759453 7 54 10.38830027799527 7 55 10.032193657785076 7 56 8.74241280101592 8 1 2.3562835219025047 8 2 2.9578623484411395 8 3 4.461033004079965 8 4 4.5842043302548205 8 5 3.6166662928635063 8 6 4.105201673706631 8 7 3.791868179340476 8 8 3.964754556494576 8 9 3.9208813938306264 8 10 3.5869868238350335 8 11 4.320059139346839 8 12 6.662479861116935 8 13 7.523490664200601 8 14 7.385669075933702 8 15 7.587212107938498 8 16 7.272390316851297 8 17 6.922983720303915 8 18 6.595941040561684 8 19 5.00654610604381 8 20 5.388045565261329 8 21 3.8717985240434913 8 22 3.633300603095684 8 23 3.6773886164839453 8 24 4.634365815336579 8 25 3.8832376712281373 8 26 2.9124784065492904 8 27 2.958664107020091 8 28 3.3541823871647694 8 29 2.994544043088581 8 30 2.858562942405335 8 31 3.4767363267264244 8 32 4.001406932931808 8 33 3.6645553129585124 8 34 3.256249546145952 8 35 3.342093026681077 8 36 1.4021872926917816 8 37 1.1555976880167016 8 38 2.2437087789748134 8 39 3.5694587456380944 8 40 -2.904872131921067 8 41 -9.680395489544308 8 42 4.782774704255644 8 43 9.02846507670652 8 44 8.162041565939571 8 45 5.575824084319827 8 46 9.110102152606142 8 47 9.176560278052975 8 48 9.566456596101741 8 49 12.471135302161954 8 50 9.273610094092867 8 51 10.49998786236905 8 52 10.003443725372655 8 53 10.646478571144069 8 54 11.411027550722544 8 55 10.65562023121165 8 56 9.539499048102165 9 1 2.9783765451583193 9 2 3.235875033430567 9 3 4.466846957568336 9 4 4.730081708690335 9 5 4.543199062419532 9 6 4.376343322755254 9 7 4.130839285753446 9 8 4.01778485952488 9 9 3.9904726552823533 9 10 3.910630234687744 9 11 4.419953431101597 9 12 6.982247302977406 9 13 7.318416668428931 9 14 7.514104593904104 9 15 7.3995799726319476 9 16 7.797584115300904 9 17 6.996274770339152 9 18 6.372191921463731 9 19 5.66088014409878 9 20 5.624479673788464 9 21 4.379021920801776 9 22 4.451482421277497 9 23 4.377881921628408 9 24 4.719108591939822 9 25 4.214985380882823 9 26 3.566283903378043 9 27 3.852603500959482 9 28 3.481208461865265 9 29 2.7376730071477766 9 30 3.5002119910311293 9 31 4.26267713010909 9 32 4.458066552382128 9 33 3.943801260809117 9 34 4.40353636785138 9 35 4.0778224135732515 9 36 2.7293543117192662 9 37 2.6425603377700497 9 38 3.199487496381437 9 39 5.417062692079249 9 40 -2.578586014937276 9 41 -4.961403241482293 9 42 5.969878298335981 9 43 10.489353025966565 9 44 9.65975122062597 9 45 6.6773039997532315 9 46 10.4577060990473 9 47 10.485933075797867 9 48 10.613496765234935 9 49 13.60415151075956 9 50 10.015858156108369 9 51 10.450304987104776 9 52 10.557178750037913 9 53 10.66973438509756 9 54 10.279773146212325 9 55 9.945789364404039 9 56 9.043903558320629 10 1 2.7538662191035215 10 2 2.99059424758525 10 3 3.959124607133397 10 4 4.588830747094974 10 5 4.203238117984945 10 6 4.016085319323096 10 7 3.6792149292826437 10 8 3.6386499994739907 10 9 3.7859635127295377 10 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115 37 5.2361042461351985 115 38 6.374079651883001 115 39 8.087663178021629 115 40 10.3782576735968 115 41 7.193982619908429 115 42 2.573141054323486 115 43 -0.1180749504305183 115 44 -1.5628566701526942 115 45 1.7345757803849544 115 46 -2.503534211030221 115 47 -6.763285945375337 115 48 -3.520947293540953 115 49 -8.064482065544967 115 50 -1.2848476173499215 115 51 1.387595279827213 115 52 1.3271660229755469 115 53 0.25841886965153194 115 54 0.7614346063534789 115 55 0.8649146681180291 115 56 3.3277170490067363 116 1 1.7864637020826848 116 2 2.197010587589375 116 3 3.346168139215102 116 4 3.4105761566266466 116 5 2.383865310062518 116 6 2.549714368219327 116 7 2.765660153132451 116 8 2.79255963429965 116 9 2.811340034289266 116 10 2.3550048418530523 116 11 0.020100089387788955 116 12 0.12419157282865001 116 13 -6.257426616716682 116 14 -9.43435549409087 116 15 -4.587849317122924 116 16 -9.20283490837393 116 17 -8.494297196977001 116 18 -2.228792784172139 116 19 -3.7018896023919012 116 20 -3.629972452756686 116 21 2.259390661635628 116 22 2.3388698086648887 116 23 2.3940143331096593 116 24 2.15258858355935 116 25 2.037005074995541 116 26 1.4835267275976136 116 27 3.1589098072657933 116 28 2.4280972110795958 116 29 2.6165751651196985 116 30 2.3982844821268756 116 31 0.9470475470376485 116 32 -8.189010757485878 116 33 0.5173399157431149 116 34 1.134627924524331 116 35 0.2958194804075305 116 36 2.4421135826180667 116 37 2.6576451900642013 116 38 3.410989696255733 116 39 4.978344904524249 116 40 7.497257270208333 116 41 4.429964870816054 116 42 1.3859688074497463 116 43 -9.978905930664485 116 44 -4.23046457656657 116 45 -1.801325792830049 116 46 -8.084615292111302 116 47 -11.42827182677913 116 48 -9.397712118066973 116 49 -9.401714820688168 116 50 -6.224137653654882 116 51 -4.2059908436096585 116 52 -4.334189362260435 116 53 -6.083251158585661 116 54 -7.165753776058784 116 55 -6.308138982547563 116 56 -3.3796246210215024 117 1 1.0951724107913918 117 2 1.6255391161179062 117 3 2.677699670746634 117 4 3.1114770575275443 117 5 2.1535349797321928 117 6 1.7572218757268274 117 7 1.9272217146940136 117 8 1.8400070817471033 117 9 1.8617904847397142 117 10 0.07032015716836781 117 11 -10.264284294996596 117 12 -11.66529791666084 117 13 -7.63610529539536 117 14 -8.648169307904684 117 15 -9.366828296101904 117 16 -10.777609683148704 117 17 -7.961864764544568 117 18 -3.2939579493373046 117 19 -2.774261974764272 117 20 -2.4008433236275586 117 21 0.816748018992989 117 22 1.073704643499724 117 23 0.17839871749404068 117 24 -2.3429069119361436 117 25 0.7958639338543989 117 26 1.6210642651351463 117 27 2.0970479454039292 117 28 1.8253945083768954 117 29 2.0622208107653464 117 30 2.245431629274023 117 31 0.6368373368274334 117 32 -12.123845592320713 117 33 1.1014239998272046 117 34 1.256249546145952 117 35 1.3057293903174383 117 36 3.04511658562107 117 37 3.482870415289426 117 38 3.767446152712189 117 39 6.411377937557283 117 40 6.087047059998124 117 41 7.025160066011249 117 42 4.18176460324554 117 43 -6.4119389636975175 117 44 -1.995029141131134 117 45 3.9455210540167975 117 46 -2.014645322141334 117 47 -8.815358913866216 117 48 -2.6562706766255317 117 49 -8.581895000868348 117 50 -2.8208343503515785 117 51 -0.2525373901562027 117 52 -0.7182734463445186 117 53 -2.175743651078154 117 54 -6.589477499782507 117 55 -3.2438747182832977 117 56 0.33899399759711457 118 1 1.4181114788917526 118 2 1.782886786368799 118 3 2.856194294402549 118 4 2.7845953370974357 118 5 2.840273330986669 118 6 2.3117021624651812 118 7 1.90428264659365 118 8 2.055419268127025 118 9 2.1435109148472407 118 10 2.4068792969533135 118 11 -7.2872233630969525 118 12 -10.770315837807793 118 13 -11.776607087510058 118 14 -1.1209291645355073 118 15 -16.909122202911938 118 16 -6.698039790675587 118 17 -9.932832506480054 118 18 -4.4763952253229675 118 19 -4.538419680857462 118 20 -3.504427553018239 118 21 0.5074290225772131 118 22 1.639654464288249 118 23 1.2174668178524684 118 24 -1.7353800302157154 118 25 1.182602285108878 118 26 1.7934656988269069 118 27 1.9278723181637858 118 28 2.0479751535381823 118 29 1.5830093412312962 118 30 2.3429226687005453 118 31 -0.13054617571737026 118 32 -8.457537348593117 118 33 -0.5580742080581018 118 34 0.17560438485563168 118 35 -0.38029211505890714 118 36 2.9146506358002817 118 37 2.98502095292384 118 38 4.1398475864039455 118 39 6.982704102431839 118 40 8.032566773259774 118 41 7.257776553466446 118 42 0.5437717717043213 118 43 1.5167348714279285 118 44 0.2874081348545303 118 45 0.8412199787479793 118 46 -4.664824533610865 118 47 -8.357652820676252 118 48 -7.967023364797575 118 49 -6.65716381807265 118 50 -5.1753146370899294 118 51 -4.401282909869465 118 52 -5.500710722330183 118 53 -6.982553686920447 118 54 -10.240731980069246 118 55 -8.111975076706239 118 56 -5.488604568711127 119 1 1.4336644742834537 119 2 1.7592692748480658 119 3 2.99674728979425 119 4 3.1924294384799268 119 5 2.722185773382982 119 6 2.190158383663338 119 7 2.0494439369162336 119 8 1.9701658119058294 119 9 2.148695246644479 119 10 2.3250820619302672 119 11 -10.034919215631515 119 12 -8.254186805549729 119 13 -1.7535656128556774 119 14 -1.5299153396507137 119 15 -6.308891788165397 119 16 -9.614514445053466 119 17 -10.526150478830285 119 18 -1.8364182667976188 119 19 -1.8500556255579212 119 20 -1.5643353871196197 119 21 -0.7483313460863812 119 22 -0.10804138824631337 119 23 -0.7831092190138946 119 24 -7.0095735786028115 119 25 -0.5783424153519476 119 26 1.2214610905319745 119 27 1.5252225485785331 119 28 1.225394508376894 119 29 1.9447604933050258 119 30 0.5831300419724315 119 31 -1.449670599680502 119 32 -12.742099560574683 119 33 -0.22397282556962494 119 34 -1.190179025282621 119 35 -1.0145087049206545 119 36 2.9411483316528155 119 37 2.8352513676703808 119 38 3.335700120966152 119 39 5.217727143906494 119 40 5.707681980633044 119 41 7.242223558074741 119 42 3.898034444515382 119 43 2.9067118299532773 119 44 0.18314546204346982 119 45 0.33718772068346325 119 46 -6.414248496744506 119 47 -8.757422405929708 119 48 -8.718175438530293 119 49 -8.89737119134454 119 50 -6.160913715430944 119 51 -8.673172310791124 119 52 -8.557162335233407 119 53 -9.924950000284502 119 54 -12.290271150576158 119 55 -11.78593821034679 119 56 -7.703466319863201 120 1 1.2073764180773665 120 2 1.9650655277754652 120 3 3.391360872932424 120 4 2.928963396325358 120 5 2.7101834314626103 120 6 2.025345737293314 120 7 2.106820986096558 120 8 1.8155990671751496 120 9 1.7918451295484559 120 10 2.2823420150918636 120 11 -8.740422728512076 120 12 -0.6886130350579265 120 13 -6.177453200677693 120 14 -4.432140164006686 120 15 -6.918376565682962 120 16 -7.638811868941055 120 17 -9.643831977659325 120 18 -3.291043559537666 120 19 -2.7473038691176406 120 20 -3.663867003044679 120 21 -0.35957256388497427 120 22 0.24383214805345688 120 23 0.5889633805177255 120 24 -4.550557185160187 120 25 -0.11961876195616838 120 26 2.0993884910003544 120 27 1.3498712605223275 120 28 1.9958863116555818 120 29 1.8815286431879343 120 30 1.4862330864689213 120 31 -1.5919422624439683 120 32 -9.585776375562972 120 33 -2.288375635874072 120 34 -10.891291437460605 120 35 -1.6658553091361128 120 36 0.3995792450017639 120 37 0.3692092131036375 120 38 -0.3411148673242437 120 39 1.756368830089162 120 40 -2.148289916322458 120 41 1.2881837454283698 120 42 -4.065958529723492 120 43 -3.773323298852345 120 44 -3.3815500883078187 120 45 -9.836992607185389 120 46 -14.044517817587598 120 47 -13.366907183447273 120 48 -11.609276141106406 120 49 -14.210310301414797 120 50 -10.364368047983637 120 51 -10.948347954819226 120 52 -10.544867253266194 120 53 -10.205980445249374 120 54 -11.152683328562107 120 55 -11.018373807536486 120 56 -7.117108006045871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2205.0 5 212.0 6 55.0 7 124.0 8 194.0 9 168.0 10 122.0 11 124.0 12 121.0 13 199.0 14 266.0 15 326.0 16 496.0 17 567.0 18 739.0 19 917.0 20 1122.0 21 1385.0 22 1699.0 23 1890.0 24 2308.0 25 2388.0 26 2552.0 27 2755.0 28 3043.0 29 3259.0 30 3575.0 31 4297.0 32 5072.0 33 6104.0 34 7336.0 35 8560.0 36 9674.0 37 8681.0 38 4698.0 39 1179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.34963579604579 0.5881554540107679 0.0475048635931774 0.014703886350269195 8 99.58037370492693 0.3042573406324933 0.061077681762656655 0.05429127267791703 9 97.21134918659638 1.5883431002104216 0.5780935357603457 0.6222141774328573 10 48.943582319142195 32.52725874315704 8.476224946839796 10.05293399086097 11 32.429589412962336 28.426648569166385 18.652867322701052 20.49089469517023 12 32.52952087951862 13.814866760168304 23.00140252454418 30.654209835768896 13 25.35402434058725 13.815997828349094 25.435461249604124 35.39451658145953 14 22.09977257555357 15.559905409533723 27.777463481970106 34.562858532942606 15 25.43219666931007 16.177636796193497 26.864166761073978 31.525999773422452 16 28.15275813295615 17.35106082036775 25.704101838755307 28.79207920792079 17 27.700661802138132 21.47632784659766 23.557893546015045 27.265116805249168 18 28.863425559351175 23.479175621564146 22.311834038413682 25.345564780670994 19 27.886007783509818 24.480722237306544 20.961172956828673 26.67209702235496 20 27.659791131578054 24.083230558604225 22.41092542514794 25.846052884669785 21 26.5332669728299 22.01773635273624 25.53559713141642 25.91339954301744 22 28.034655929059404 20.6159797312582 25.870922499208255 25.47844184047414 23 28.316291906076096 22.205130525268064 25.98176718092567 23.49681038773017 24 26.740713930235714 21.39980998054563 25.14477672714111 26.71469936207755 25 26.653093541454588 21.776948309014816 25.730121027033142 25.839837122497457 26 25.087246192893403 20.773658448150833 27.74655547498187 26.392539883973893 27 23.294490326960062 17.66489421880303 30.143681411924426 28.896934042312477 28 22.691170305577902 21.129856445235045 31.635300649225574 24.54367259996148 29 23.965698641295123 19.22550428201328 32.759381398979556 24.049415677712034 30 22.5395460408699 19.35210120165652 35.98746294326642 22.120889814207157 31 24.04529533784473 19.915893041339363 34.69468720599404 21.34412441482187 32 26.6527149321267 16.343891402714934 34.30882352941176 22.694570135746606 33 24.476522842639596 17.741569978245106 37.11702320522117 20.664883973894128 34 22.71327658032965 19.335265350479986 39.698424198514765 18.2530338706756 35 21.882462116496722 21.074431605988934 40.22837613028077 16.81473014723357 36 21.503829247292337 21.589930665699914 38.524720170390175 18.381519916617574 37 21.87379543341723 21.20263927623971 37.81602158583316 19.107543704509897 38 23.353232365568253 22.222725676548215 37.69299606928035 16.73104588860318 39 21.670526267992553 17.40339644916678 33.484311855787126 27.441765427053532 40 13.406848520023216 14.433329919315376 27.240449284763237 44.91937227589817 41 15.69278138898392 16.36788687421599 29.679552970692214 38.259778766107885 42 19.53937003411654 20.785020458589774 38.81918233647296 20.856427170820723 43 12.327041169593299 12.759509188526543 30.599666284520822 44.313783357359334 44 13.977553132213643 13.586381778692532 28.231427889152954 44.20463719994087 45 19.769667183801964 19.517139071647772 39.649178434325705 21.06401531022456 46 19.509406624255735 20.393470829277128 39.1127662946277 20.984356251839444 47 18.325620339673453 18.77821654465427 36.84359406646375 26.052569049208525 48 20.10026367309063 20.99426256407935 39.62225717745312 19.283216585376895 49 14.239662198208833 14.264634104813902 27.99804765093815 43.49765604603911 50 18.639475832048607 20.81595175478371 40.929083159517546 19.615489253650132 51 20.527516428141777 22.03584115490688 37.96915254622011 19.467489870731235 52 19.169590244899346 21.66626928710106 38.4924554651044 20.67168500289519 53 19.507219439218442 21.811319899358526 39.52897975836979 19.152480903053245 54 17.40908112760921 19.426228579842117 34.71509632700214 28.449593965546534 55 19.437111229449687 21.648783693447694 39.11215853340736 19.801946543695262 56 20.034452276796845 22.278383462907136 36.712074163059 20.97509009723702 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 1.0 8 3.0 9 5.0 10 9.0 11 13.0 12 51.0 13 127.0 14 165.0 15 219.5 16 274.0 17 376.0 18 478.0 19 580.5 20 683.0 21 823.5 22 1208.5 23 1453.0 24 1820.5 25 2188.0 26 2458.0 27 2728.0 28 2955.0 29 3314.5 30 3447.0 31 3253.5 32 3060.0 33 2789.5 34 2519.0 35 2283.5 36 2048.0 37 1829.0 38 1558.0 39 1506.0 40 1508.5 41 1511.0 42 1540.5 43 1570.0 44 1621.0 45 1672.0 46 1897.5 47 2174.0 48 2225.0 49 2416.5 50 2608.0 51 2797.5 52 2987.0 53 3221.5 54 3865.5 55 4275.0 56 4688.5 57 5102.0 58 5517.0 59 5932.0 60 6136.5 61 6341.0 62 6112.0 63 5338.0 64 4793.0 65 4218.0 66 3643.0 67 3134.5 68 2626.0 69 2273.0 70 1920.0 71 1623.0 72 1139.5 73 953.0 74 760.5 75 568.0 76 415.0 77 262.0 78 204.5 79 98.0 80 49.0 81 35.0 82 21.0 83 12.0 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.020359227254218886 10 0.0 11 0.0022621363615798764 12 0.0 13 0.0 14 0.03506311360448808 15 0.1606116816721712 16 0.0418495226892277 17 0.019228159073428945 18 0.006786409084739629 19 0.02262136361579876 20 0.03506311360448808 21 0.006786409084739629 22 0.0 23 0.0 24 0.0 25 0.0022621363615798764 26 0.17644663620323034 27 0.024883499977378636 28 0.1730534316608605 29 0.02149029543500882 30 0.03845631814685789 31 0.21603402253087817 32 0.013572818169479257 33 0.17644663620323034 34 0.08596118174003528 35 0.05542234085870696 36 0.1628738180337511 37 0.23300004524272724 38 0.15043206804506173 39 0.3619418178527802 40 0.6096457494457765 41 0.8166312265303353 42 0.20924761344613854 43 0.3540243405872506 44 0.5327331131520608 45 0.11876215898294348 46 0.07917477265529566 47 0.03732524996606795 48 0.0508980681355472 49 0.3540243405872506 50 0.22168936343482784 51 0.3404515224177714 52 0.3789078405646293 53 0.20133013618060897 54 0.1345971135140026 55 0.1730534316608605 56 0.19680586345744924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 88412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.88639551192145 #Duplication Level Percentage of deduplicated Percentage of total 1 93.3882683745997 81.14170022168936 2 4.423442422348929 7.686739356648419 3 1.0140852404384388 2.6433063385060853 4 0.42698325913197427 1.4839614531963985 5 0.2265094118565961 0.9840293172872461 6 0.12627248821890702 0.658281681219744 7 0.0937280324923846 0.5700583631181287 8 0.0455622380171314 0.3166990906211826 9 0.03254445572652243 0.254490340677736 >10 0.20437918196256083 3.0538840881328326 >50 0.014319560519669868 0.8121069538071756 >100 0.0039053346871826917 0.3947427950956883 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGTACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 129 0.145907795321902 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 118 0.13346604533321269 No Hit AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 102 0.11536895444057367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.00565534090394969 0.0 0.0 0.0 0.0 11 0.006786409084739628 0.0 0.0 0.0 0.0 12 0.01131068180789938 0.0 0.0 0.0 0.0 13 0.013572818169479256 0.0 0.0 0.0 0.0 14 0.015834954531059132 0.0 0.0 0.0 0.0 15 0.018097090892639008 0.0 0.0 0.0 0.0 16 0.019228159073428945 0.0 0.0 0.0 0.0 17 0.020359227254218883 0.0 0.0 0.0 0.0 18 0.02262136361579876 0.0 0.0 0.0 0.0 19 0.026014568158168574 0.0 0.0 0.0 0.0 20 0.02827670451974845 0.0 0.0 0.0 0.0 21 0.02940777270053839 0.0 0.0 0.0 0.0 22 0.036194181785278015 0.0 0.0 0.0 0.0 23 0.03732524996606795 0.0 0.0 0.0 0.0 24 0.03732524996606795 0.0 0.0 0.0 0.0 25 0.04184952268922771 0.0 0.0 0.0 0.0 26 0.0475048635931774 0.0 0.0 0.0 0.0 27 0.048635931773967335 0.0 0.0 0.0 0.0 28 0.053160204497127085 0.0 0.0 0.0 0.0 29 0.0565534090394969 0.0 0.0 0.0 0.0 30 0.0565534090394969 0.0 0.0 0.0 0.0 31 0.0565534090394969 0.0 0.0 0.0 0.0 32 0.0565534090394969 0.0 0.0 0.0 0.0 33 0.0565534090394969 0.0 0.0 0.0 0.0 34 0.0565534090394969 0.0 0.0 0.0 0.0 35 0.0565534090394969 0.0 0.0 0.0 0.0 36 0.0565534090394969 0.0 0.0 0.0 0.0 37 0.05768447722028684 0.0 0.0 0.0 0.0 38 0.05768447722028684 0.0 0.0 0.0 0.0 39 0.05768447722028684 0.0 0.0 0.0 0.0 40 0.05768447722028684 0.0 0.0 0.0 0.0 41 0.06220874994344659 0.0 0.0 0.0 0.0 42 0.06333981812423653 0.0 0.0 0.0 0.0 43 0.06333981812423653 0.0 0.0 0.0 0.0 44 0.06673302266660634 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACC 15 0.008022008 50.593445 38 AAATAAT 15 0.00818751 50.33295 35 TATGCCT 15 0.00818751 50.33295 35 AAAGGCT 20 4.0284457E-4 50.33295 35 AAGGCTA 20 4.0284457E-4 50.33295 36 ATATGCT 45 1.2732926E-10 50.275433 34 TCGACCC 15 0.00822463 50.27543 34 CCAGGTC 15 0.008280545 50.18939 43 CATTGAC 15 0.008280545 50.18939 43 CGATTAA 15 0.008280545 50.18939 49 CAAGGAT 15 0.008280545 50.18939 49 ACCAGCG 15 0.008280545 50.18939 42 CTGCAAT 35 5.06152E-8 50.18939 49 CACCGCG 15 0.008280545 50.18939 44 CCTTGAC 15 0.008280545 50.18939 43 CAACGAT 15 0.0082992455 50.160774 46 AAGAATG 15 0.0082992455 50.160774 48 ACGATTA 15 0.0082992455 50.160774 48 ACCGCGC 15 0.008317978 50.132195 45 TCCACGA 15 0.008317978 50.132195 45 >>END_MODULE