FastQCFastQC Report
Fri 17 Jun 2016
SRR1526278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49248
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC720.14619883040935672No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC680.13807667316439245No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA670.1360461338531514No Hit
ATCAACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT580.1177712800519818No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC540.10964912280701754No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC520.1055880441845354No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG500.10152696556205328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCT150.00802698650.55154439
ACAAACC251.940285E-550.4994838
ACCGGCC150.00805979650.4994838
ACCTCCC150.00805979650.4994838
AAACCAC204.0064086E-450.3439437
TTGCCTA251.988172E-550.2923142
CCTTGTC204.0472476E-450.2407843
AAGGCTA252.0002954E-550.2407836
TGCACCG150.00822534550.24077636
TTTTCGT150.00822534550.24077636
AAAGGCT204.0677906E-450.18935435
CCTGCGA204.0884168E-450.1380448
CTTCAAC150.00829226850.13803549
TAGCAAG252.024726E-550.13803547
CTAGCAA252.024726E-550.13803546
TAGAGCC150.00829226850.13803534
AGCAAGC301.0078966E-650.13803548
TCCTGCA150.008427327549.93380733
TGGTTGA150.00846134749.8830114
GGTTGAT150.00846134749.8830115