FastQCFastQC Report
Fri 17 Jun 2016
SRR1526277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23145
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC530.2289911427954202No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC450.19442644199611148No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC370.15986174119680277No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC300.12961762799740764No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC280.12097645279758047No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG270.1166558651976669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGC150.0079519450.58461437
AACCAAC203.911252E-450.47368241
CTTGCCT150.00802147750.47368241
CGTCCTG150.00809146550.3632436
TGCAATC150.00823280150.1437950
ACCGCTG150.00823280150.1437942
ACCAACA204.0404464E-450.1437942
TTGCCTA150.00823280150.1437942
CAAGCGC150.00823280150.1437950
CGCTGCA150.008304154550.03478244
CCAACAC204.0842564E-450.03478243
CCTGCGC150.008304154550.03478243
GTTCCTG150.008304154550.03478211
CCAAAAC204.0842564E-450.03478243
AAGAGTG150.008304154550.03478210
CAACACA204.0842564E-450.03478244
TTCCTGC150.008304154550.03478212
TGCCTAG150.008304154550.03478243
GTAAGAC204.0842564E-450.03478234
TCCTGCT150.008304154550.03478213