##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526277_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23145 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.92089004104558 39.0 38.0 39.0 35.0 40.0 2 36.95195506588896 39.0 38.0 39.0 32.0 40.0 3 37.77066321019659 39.0 38.0 39.0 35.0 40.0 4 37.57723050334845 39.0 38.0 39.0 35.0 40.0 5 37.23694102397926 39.0 37.0 39.0 33.0 40.0 6 37.38647656081227 39.0 38.0 39.0 35.0 40.0 7 36.83046014257939 38.0 36.0 39.0 33.0 40.0 8 36.83460790667531 38.0 36.0 39.0 33.0 40.0 9 36.890343486714194 38.0 37.0 39.0 33.0 40.0 10 36.97736012097645 38.0 37.0 39.0 33.0 40.0 11 35.46593216677468 38.0 35.0 39.0 29.0 40.0 12 33.22086843810758 37.0 31.0 39.0 20.0 40.0 13 32.54357312594513 36.0 30.0 39.0 17.0 40.0 14 32.2199611147116 36.0 29.0 39.0 16.0 40.0 15 32.804709440483904 36.0 30.0 39.0 18.0 40.0 16 32.50308922013394 36.0 30.0 38.0 19.0 40.0 17 32.40259235255995 36.0 30.0 38.0 18.0 39.0 18 32.49600345647008 36.0 30.0 38.0 19.0 39.0 19 33.31380427738172 36.0 31.0 38.0 24.0 39.0 20 33.56750918124865 36.0 31.0 38.0 24.0 40.0 21 34.86238928494275 38.0 34.0 39.0 27.0 40.0 22 34.522618276085545 38.0 33.0 39.0 25.0 40.0 23 34.73847483257723 38.0 33.0 39.0 27.0 40.0 24 34.35251674227695 38.0 33.0 39.0 25.0 40.0 25 34.603456470079934 38.0 33.0 39.0 26.0 40.0 26 34.80276517606394 38.0 35.0 39.0 27.0 40.0 27 34.76254050550875 38.0 35.0 39.0 27.0 40.0 28 34.68576366385828 38.0 34.0 39.0 27.0 40.0 29 34.47051198963059 38.0 34.0 39.0 26.0 40.0 30 34.27716569453445 38.0 33.0 39.0 25.0 40.0 31 34.05893281486282 38.0 33.0 39.0 25.0 39.0 32 33.01589976236768 37.0 32.0 39.0 18.0 39.0 33 34.3202419529056 38.0 34.0 39.0 26.0 40.0 34 33.993648736228124 38.0 33.0 39.0 24.0 40.0 35 33.94672715489307 38.0 33.0 39.0 25.0 40.0 36 31.887751134154247 35.0 31.0 38.0 19.0 39.0 37 31.623849643551523 35.0 30.0 38.0 18.0 39.0 38 30.801123352775978 35.0 30.0 38.0 11.0 39.0 39 28.93799956794124 33.0 26.0 38.0 4.0 39.0 40 24.667617195938647 30.0 11.0 36.0 4.0 39.0 41 21.453229639230937 25.0 8.0 33.0 4.0 37.0 42 25.1699287103046 30.0 19.0 34.0 4.0 37.0 43 21.431324260099373 25.0 7.0 33.0 4.0 36.0 44 22.454266580254913 27.0 9.0 33.0 4.0 36.0 45 24.82665802549147 30.0 18.0 34.0 4.0 37.0 46 24.883732987686326 30.0 12.0 36.0 4.0 38.0 47 24.195981853532082 30.0 7.0 35.0 4.0 38.0 48 24.103650896521927 29.0 8.0 35.0 4.0 38.0 49 21.695744221214085 26.0 4.0 35.0 4.0 38.0 50 23.88852883992223 29.0 4.0 35.0 4.0 38.0 51 25.334283862605314 32.0 4.0 38.0 4.0 39.0 52 25.135580038885287 32.0 4.0 38.0 4.0 39.0 53 24.722704687837545 31.0 4.0 37.0 4.0 39.0 54 23.834089436163318 30.0 4.0 37.0 4.0 39.0 55 24.052927198098942 31.0 4.0 37.0 4.0 39.0 56 23.079196370706416 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9295964105761385 1 2 2.447477762760961 1 3 1.4179572306338173 1 4 1.3473586382256713 1 5 1.582300863601283 1 6 1.4937163461735636 1 7 2.3327468198268946 1 8 2.7074992315573994 1 9 2.2969523258875952 1 10 1.7879533792009923 1 11 -5.02386819681389 1 12 -8.289637591172177 1 13 -20.86258682040432 1 14 -17.9853545796673 1 15 -12.299874530209614 1 16 -20.76555714760891 1 17 -21.334372142447656 1 18 -19.419642107297143 1 19 -7.027186322871085 1 20 -11.324256771476989 1 21 -5.212494921323312 1 22 -2.462822120126983 1 23 -3.6148784080417506 1 24 -2.3800959748738606 1 25 -3.500240854662291 1 26 1.4689984806507965 1 27 1.7763705878179081 1 28 2.288294937623519 1 29 2.37133718644413 1 30 2.081187382376605 1 31 0.4122825744862766 1 32 -11.374687532345416 1 33 -0.7050391088832555 1 34 0.5865750135899717 1 35 0.16427419129140475 1 36 4.268815013676143 1 37 4.403724871570315 1 38 5.614644131319885 1 39 7.92821631861198 1 40 10.158437067771654 1 41 -3.4449258008055246 1 42 -10.635295691375475 1 43 -14.030602256481489 1 44 -15.902127207703543 1 45 -16.142294205027447 1 46 -18.169480306736116 1 47 -17.288146984797425 1 48 -16.60540148139436 1 49 -13.727998888554627 1 50 -16.144984167007376 1 51 -16.810235135400696 1 52 -16.610197052584528 1 53 -16.345354249325155 1 54 -17.296700276463966 1 55 -16.08084581269334 1 56 -14.883000182968871 2 1 0.6524572024024451 2 2 2.0668902787252037 2 3 1.0667439483860477 2 4 1.0510623419293736 2 5 1.3524158061300184 2 6 1.1207916973868493 2 7 1.8665910088434998 2 8 1.74325912938626 2 9 1.9738361062196503 2 10 1.220903570771874 2 11 -3.3252730499428793 2 12 -12.227057769971665 2 13 -14.739981456419645 2 14 -14.261216648632814 2 15 -5.838827276058911 2 16 -3.667217428579537 2 17 -2.1044870849763875 2 18 -9.638032911894847 2 19 -2.8994724020537177 2 20 -3.085431739548511 2 21 1.9803530991109142 2 22 3.5078036780850184 2 23 2.173116483401415 2 24 2.6722667326612637 2 25 2.839478174711914 2 26 2.180365019603542 2 27 2.3370347002061536 2 28 1.8745018341752413 2 29 2.357288655154221 2 30 2.129718672287204 2 31 0.7979786153547366 2 32 -9.018365693264954 2 33 0.3307207889456052 2 34 0.8739313354290488 2 35 1.0391145488903817 2 36 3.2815864057578814 2 37 3.8749892393864123 2 38 6.181693939749003 2 39 8.073810188343785 2 40 9.397262099700132 2 41 6.747922219628705 2 42 2.459212610029379 2 43 -3.2847529589080526 2 44 -11.206086339248882 2 45 2.318753049123252 2 46 -9.722481583875325 2 47 -7.074864737032419 2 48 -6.60540148139436 2 49 -12.994920983062928 2 50 -5.086235763431386 2 51 -6.709341137954976 2 52 -6.092955673274183 2 53 -6.857487071802806 2 54 -14.232843316055284 2 55 -6.333719375911732 2 56 -4.4079043975282595 3 1 0.8555223365020623 3 2 2.262292577575778 3 3 1.4920313047078864 3 4 1.6066178974849308 3 5 1.8045230858235044 3 6 1.4196422720994946 3 7 1.6290431161231922 3 8 2.94823997229814 3 9 2.8339893629246333 3 10 1.7601756014232137 3 11 2.1335392105935185 3 12 3.4788809273463457 3 13 -1.3625868204043208 3 14 -3.5686879130006304 3 15 0.1630884327533515 3 16 4.465924333872572 3 17 4.24896119088568 3 18 3.3859134482584103 3 19 3.4357766400918806 3 20 2.2220395248193086 3 21 4.3893569305285425 3 22 4.277918620613754 3 23 4.283269740106398 3 24 4.10138550660762 3 25 4.814573960152522 3 26 3.617146628798949 3 27 3.183777995225313 3 28 3.2327393820679617 3 29 3.4176334827404276 3 30 3.22933553052475 3 31 3.6437640559677575 3 32 3.9586458009879166 3 33 3.785701631857485 3 34 3.058797235812193 3 35 4.405014932032145 3 36 5.926222421083548 3 37 6.292613760459208 3 38 7.197977464653221 3 39 9.511549651945316 3 40 12.760288919623502 3 41 13.240259384379659 3 42 10.457296901217116 3 43 4.423101447222216 3 44 6.384909829333491 3 45 8.709557646824404 3 46 1.6175567303009224 3 47 -3.1399988366492764 3 48 2.616820740827862 3 49 -8.987258147813886 3 50 0.567978795955586 3 51 -0.8472721724377337 3 52 0.5286918363043611 3 53 0.4231642691933608 3 54 -1.1763299060935992 3 55 0.0024875206399919136 3 56 3.700333150364461 4 1 -0.23177925079952644 4 2 1.8337211490043472 4 3 1.1586979713745578 4 4 1.4558242466912787 4 5 0.8203961016965167 4 6 1.1339279863852099 4 7 1.8353923224723943 4 8 2.1783987024568674 4 9 2.3974814264166966 4 10 1.1609692522168658 4 11 3.153380480434791 4 12 2.1653888638542824 4 13 4.2485242907067935 4 14 2.1098835155707967 4 15 6.051977321642237 4 16 4.87465449260273 4 17 5.856104048028534 4 18 5.913691226036189 4 19 4.562760767076007 4 20 4.0196585724383525 4 21 3.7028489940206057 4 22 3.6112519539470895 4 23 2.9062856131222716 4 24 3.7601156653377785 4 25 2.401875547454111 4 26 1.5695275811798979 4 27 2.6202859317332496 4 28 3.002580651909234 4 29 2.5723953875023327 4 30 2.070605371794592 4 31 2.3382085004122075 4 32 3.577693420035537 4 33 2.5753841715400227 4 34 2.562765489780446 4 35 2.3256498526670626 4 36 4.239714484575611 4 37 4.737058204903651 4 38 6.7416282583040115 4 39 7.920279810675474 4 40 11.09759050692509 4 41 5.065656209776488 4 42 7.94936039328061 4 43 7.522307796428564 4 44 9.14284633727 4 45 7.876224313491068 4 46 7.073905936650128 4 47 6.923493226842787 4 48 5.7993604233675455 4 49 4.734964074408335 4 50 7.159248637225428 4 51 4.247965922800361 4 52 5.723136280748804 4 53 4.347767443796538 4 54 0.9149399351762426 4 55 5.871535139687612 4 56 5.482079182110493 5 1 0.8555223365020623 5 2 2.7067370220202207 5 3 1.825364638041222 5 4 1.5788401197071522 5 5 1.332300863601283 5 6 1.4751978276550446 5 7 2.323487560567635 5 8 2.19823997229814 5 9 2.472878251813526 5 10 1.2601756014232137 5 11 3.716872543926854 5 12 5.978880927346346 5 13 6.665190957373458 5 14 6.125756531443816 5 15 6.468643988308909 5 16 6.5492576672059 5 17 5.860072301996794 5 18 5.6914690038139675 5 19 5.130221084536323 5 20 4.94426174704153 5 21 3.778245819417428 5 22 3.9445852872804252 5 23 3.4499364067730625 5 24 4.129163284385399 5 25 4.370129515708079 5 26 2.283813295465613 5 27 2.1560002174475343 5 28 1.9827393820679617 5 29 4.389855704962649 5 30 1.923779974969193 5 31 2.9770973893010932 5 32 4.0975346898768095 5 33 1.8134794096352636 5 34 2.9199083469233003 5 35 2.4605704875877024 5 36 4.398444643305769 5 37 4.264835982681429 5 38 6.920199686875442 5 39 8.67821631861198 5 40 9.28806669740128 5 41 3.851370495490773 5 42 9.207296901217116 5 43 13.311990336111105 5 44 9.468243162666827 5 45 8.237335424602179 5 46 9.562001174745365 5 47 11.110001163350724 5 48 10.700154074161198 5 49 9.179408518852782 5 50 9.734645462622254 5 51 8.09717227200671 5 52 9.778691836304358 5 53 8.31205315808225 5 54 8.434781205017515 5 55 10.085820853973324 5 56 10.33922203925335 6 1 1.5287249508811485 6 2 2.252488656007145 6 3 1.913599932158867 6 4 2.1981211654587867 6 5 1.6019087067385342 6 6 2.174544232883804 6 7 2.632311089979403 6 8 2.1671942206641504 6 9 2.905884787761238 6 10 1.8304370393317129 6 11 4.282232021051037 6 12 6.088358051529347 6 13 7.003426251491103 6 14 6.426410126215057 6 15 7.150016537328515 6 16 6.2780158371405435 6 17 6.351902367356267 6 18 6.031338284859718 6 19 4.883489058392534 6 20 4.609294426780089 6 21 2.7896837279141593 6 22 3.4641931304176765 6 23 3.5757534002371187 6 24 3.790927990267754 6 25 3.6315674242048104 6 26 1.6367544719362002 6 27 2.1707060998004764 6 28 2.347118467035287 6 29 2.4617511297992536 6 30 2.1966557919626624 6 31 2.7107575200200458 6 32 4.468449722556549 6 33 2.8134794096352636 6 34 2.576771092021339 6 35 2.79063584706482 6 36 1.9245884341554422 6 37 1.9723523225507087 6 38 3.3074545888362223 6 39 5.1569744885466235 6 40 1.3272823836757937 6 41 0.38078226019665706 6 42 5.235074678994895 6 43 11.666565499509797 6 44 10.866935973124342 6 45 8.248773333098914 6 46 7.900236468863014 6 47 10.480916196030464 6 48 9.39459851860564 6 49 11.372218976369119 6 50 8.442161802491533 6 51 7.152727827562266 6 52 7.742744123886059 6 53 7.52283747180774 6 54 8.570402120050193 6 55 5.781899285345876 6 56 6.476476941214134 7 1 0.7999667809465052 7 2 2.012292577575778 7 3 1.325364638041222 7 4 1.8843956752627093 7 5 1.2767453080457258 7 6 1.4751978276550446 7 7 2.4345986716787493 7 8 2.69823997229814 7 9 2.778433807369076 7 10 1.621286712534328 7 11 3.1335392105935185 7 12 6.534436482901896 7 13 6.581857624040122 7 14 6.736867642554923 7 15 5.996421766086687 7 16 6.577035444983679 7 17 5.693405635330123 7 18 4.969246781591746 7 19 4.546887751202988 7 20 4.638706191485973 7 21 2.5560235971952068 7 22 2.9445852872804252 7 23 2.866603073439734 7 24 2.9624966177187346 7 25 1.7312406268191936 7 26 1.478257739910056 7 27 1.6282224396697558 7 28 2.6216282709568546 7 29 2.639855704962649 7 30 2.812668863858086 7 31 2.2826529448566504 7 32 3.9030902454323666 7 33 3.424590520746378 7 34 3.503241680256636 7 35 2.7661260431432524 7 36 0.7040001988613263 7 37 0.37594709379254 7 38 -0.02424475756900435 7 39 3.0393274297230946 7 40 -1.9063777470431624 7 41 -8.98196283784256 7 42 3.429519123439338 7 43 7.173101447222216 7 44 6.440465384889048 7 45 3.209557646824404 7 46 6.562001174745365 7 47 6.0544456077951665 7 48 7.172376296383419 7 49 8.929408518852782 7 50 6.873534351511143 7 51 8.236061160895598 7 52 5.389802947415472 7 53 7.034275380304475 7 54 6.434781205017515 7 55 5.752487520639992 7 56 5.866999817031129 8 1 0.8656233466030727 8 2 2.5653228806060753 8 3 1.189001001677589 8 4 1.2631835540505847 8 5 1.0974523787527986 8 6 1.2681271205843387 8 7 1.8916693787494552 8 8 2.1048056288637937 8 9 2.323888352823623 8 10 0.6263372175848332 8 11 2.78757961463392 8 12 4.572315270780685 8 13 6.006100048282551 8 14 6.651009056696335 8 15 5.794401564066479 8 16 7.099762717710952 8 17 5.150476342400829 8 18 5.459145771490736 8 19 4.824665528980766 8 20 3.8205243733041527 8 21 3.1141044052760165 8 22 3.8536761963713317 8 23 1.6140778209144813 8 24 2.303405708627821 8 25 2.4256850712636364 8 26 0.9201769318292463 8 27 1.6181214295687454 8 28 1.1670828164113942 8 29 1.7282395433464899 8 30 1.0879213891106119 8 31 2.277602439806145 8 32 3.3222821646242835 8 33 1.5710551672110213 8 34 2.1017265287414872 8 35 0.925216952234166 8 36 -0.3338785890174627 8 37 0.16130062914607635 8 38 0.9580784747542275 8 39 2.615085005480669 8 40 -4.181630272295688 8 41 -10.57287192875165 8 42 4.250226194146411 8 43 8.875121649242416 8 44 6.326829021252685 8 45 5.073194010460767 8 46 7.281698144442334 8 47 8.031718335067893 8 48 8.637022761029883 8 49 10.71981255925682 8 50 8.949291927268717 8 51 11.001212676047118 8 52 10.54131809893062 8 53 9.546901642930735 8 54 10.598922619158927 8 55 9.555517823670296 8 56 9.581646281677592 9 1 1.3029697839495142 9 2 1.7127430280262246 9 3 1.446986259662843 9 4 0.8078190986861316 9 5 1.0327513140517297 9 6 1.0592819117391343 9 7 2.587001074081151 9 8 1.6584501825083535 9 9 2.0577130866483557 9 10 1.7008662921139077 9 11 3.984139811194119 9 12 6.063715762181175 9 13 6.293569335751837 9 14 6.501131906819186 9 15 6.263689033353952 9 16 6.1603687783170145 9 17 6.188150380074866 9 18 5.7515290638740275 9 19 4.9327736370888715 9 20 4.179246732026513 9 21 3.543260834432445 9 22 3.9355762782714123 9 23 3.5272637341003943 9 24 3.689974095196213 9 25 3.452712098290661 9 26 2.8243538360061535 9 27 2.622216433663752 9 28 2.6396462889748733 9 29 3.586552401659347 9 30 3.298404599593823 9 31 2.7075778697815736 9 32 4.589276431618551 9 33 3.4351010312568846 9 34 3.442430869445822 9 35 3.59270261971983 9 36 3.3188650637261894 9 37 2.980301448146893 9 38 3.5665960832718326 9 39 5.633922024317691 9 40 0.7970757064102933 9 41 -4.175656531536255 9 42 5.789128733048948 9 43 10.865293639414407 9 44 9.987762682186347 9 45 6.448296385563143 9 46 10.20389306663726 9 47 10.196337499687058 9 48 10.232436356443479 9 49 11.144873984318245 9 50 10.247408225385016 9 51 10.936511611346049 9 52 10.416829974442496 9 53 10.188179284208378 9 54 10.33192835216466 9 55 9.306541574694045 9 56 8.411294111325425 10 1 0.7346073038223224 10 2 2.752488656007145 10 3 1.472423461570635 10 4 2.1687094007529026 10 5 2.2195557655620632 10 6 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1.5467958479850665 114 31 2.3858275480312514 114 32 -0.5175446752025579 114 33 -0.5198539236980722 114 34 2.015146442161395 114 35 2.7304117574289677 114 36 4.596857341718472 114 37 5.213248681094125 114 38 5.408294924970676 114 39 6.75361314400881 114 40 12.026161935496521 114 41 7.232322876443153 114 42 3.4493603932806103 114 43 2.736593510714279 114 44 -2.9762012817776196 114 45 4.042890980157736 114 46 -3.1641893014451092 114 47 -6.266982963633405 114 48 -4.033972909965787 114 49 -12.669797830353568 114 50 -3.102656124679335 114 51 -3.6091769343424964 114 52 -2.2292446716321486 114 53 -2.4141373181082244 114 54 -4.2279172076809 114 55 -1.7951315269790555 114 56 1.8630315630628758 115 1 0.4666334476131766 115 2 1.873403688686885 115 3 0.8253646380412221 115 4 0.9955067863738165 115 5 1.2489675302679473 115 6 1.2529756054328232 115 7 1.8512653383454136 115 8 2.3926844167425827 115 9 1.611767140702412 115 10 0.6768422680898851 115 11 0.49465032170462564 115 12 -1.0488968504314364 115 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0.3193944942289342 115 52 1.3342473918599147 115 53 -1.8823912863621928 115 54 -1.0929965727602635 115 55 0.808043076195549 115 56 2.172555372586686 116 1 0.7640190685282064 116 2 2.223076891301268 116 3 0.8253646380412221 116 4 1.4040035183999606 116 5 1.4842616479150053 116 6 1.380426585824985 116 7 1.897016972332338 116 8 2.5201353971347444 116 9 2.229414199525941 116 10 1.3304370393317129 116 11 0.4881143739922109 116 12 -1.941053713176533 116 13 -7.967161983803013 116 14 -11.044178109079063 116 15 -5.173512874436192 116 16 -9.36904298638887 116 17 -12.530450573820204 116 18 -3.20395583278734 116 19 -4.351805059254527 116 20 -4.596587926161085 116 21 1.0838013749729853 116 22 0.9641931304176765 116 23 1.2522239884724087 116 24 -0.2973073038498981 116 25 1.3668615418518684 116 26 2.872048589583258 116 27 2.5824708056828314 116 28 2.288294937623519 116 29 2.0205746592110145 116 30 2.3143028507861914 116 31 -0.2892424799799542 116 32 -14.060962042149335 116 33 -1.774755884482385 116 34 0.07677109202133892 116 35 -0.5917170941116439 116 36 4.571647257684855 116 37 4.560587616668354 116 38 6.307454588836222 116 39 8.156974488546624 116 40 9.356694148381674 116 41 6.616076377843715 116 42 4.1468393848772465 116 43 -10.215787441666674 116 44 0.16105362018316427 116 45 -3.486520784548148 116 46 -5.335057648784048 116 47 -7.872024980440127 116 48 -12.075989716688477 116 49 -3.51013396480735 116 50 -5.9990146680967005 116 51 -5.729625113614205 116 52 -5.316079405525706 116 53 -3.624221351721669 116 54 -6.547244938773336 116 55 -5.629865420536479 116 56 -2.464699529374098 117 1 0.41107789205761947 117 2 2.012292577575778 117 3 0.9086979713745578 117 4 1.1343956752627093 117 5 1.082300863601283 117 6 1.0029756054328232 117 7 1.9068208939009708 117 8 2.059351083409254 117 9 1.6951004740357476 117 10 1.1490644903121066 117 11 -9.64423856718426 117 12 -11.2155635170981 117 13 -14.584809042626542 117 14 -13.985354579667298 117 15 -10.281356011691095 117 16 -14.089631221682986 117 17 -11.167705475780988 117 18 -4.836308773963811 117 19 -3.8420011376859016 117 20 -5.027960475180695 117 21 -1.082865291693679 117 22 0.11125195394708953 117 23 0.477714184550841 117 24 -1.2041700489479332 117 25 -0.4076482620696993 117 26 2.783813295465613 117 27 3.1560002174475343 117 28 2.3716282709568546 117 29 3.334300149407092 117 30 2.2015577527469716 117 31 -0.6617914995877925 117 32 -14.79135419901208 117 33 -0.9365205903647364 117 34 1.8365750135899717 117 35 -1.150540623523412 117 36 3.4540001988613263 117 37 4.737058204903651 117 38 3.3368663535421064 117 39 6.7893274297230946 117 40 6.871400030734616 117 41 5.851370495490773 117 42 3.068408012328227 117 43 -7.88245410833334 117 44 0.6071320515557161 117 45 1.459557646824404 117 46 -3.271332158587967 117 47 -7.4455543922048335 117 48 -2.3554014813943596 117 49 -7.015035925591663 117 50 -5.459798981822191 117 51 -1.6806055057710658 117 52 -1.276863719251196 117 53 -1.18794684191775 117 54 -5.204107683871374 117 55 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-0.7427682617885338 120 2 0.9289592442424421 120 3 -0.713096900420318 120 4 -0.48739919653216646 120 5 0.24896753026794727 120 6 -0.37522952277230104 120 7 -0.46497397789390504 120 8 -1.3124437883856217 120 9 -0.042079013143741406 120 10 -2.5881149968673824 120 11 -14.9327001056458 120 12 -5.959153260687845 120 13 -13.700193658011159 120 14 -10.369969964282681 120 15 -7.49930472963981 120 16 -8.608861990913756 120 17 -11.847192655268167 120 18 -4.7786164662715045 120 19 -5.175334471019234 120 20 -7.5151399623601804 120 21 -3.070044778873168 120 22 -1.3118249691298303 120 23 -0.9966447898081299 120 24 -7.120836715614601 120 25 -2.497391851813287 120 26 -1.254648242995927 120 27 -1.9016920902447723 120 28 -0.5578589085303278 120 29 -3.8708280557211125 120 30 -4.984339683150463 120 31 -5.764355602151898 120 32 -14.938790096447978 120 33 -6.9557513595955065 120 34 -16.439066012051057 120 35 -7.881309854292642 120 36 -8.007538262677134 120 37 -7.955249487404043 120 38 -7.24005672338097 120 39 -4.114518724123059 120 40 -8.801676892342307 120 41 -2.2255525814323036 120 42 -7.264925321005105 120 43 -3.2927105185897503 120 44 -8.001842307418643 120 45 -7.8032628659961105 120 46 -14.611075748331558 120 47 -14.695554392204834 120 48 -10.60540148139436 120 49 -14.098369258924997 120 50 -8.062363084386295 120 51 -9.078041403206964 120 52 -8.533273975661452 120 53 -9.57897248294339 120 54 -10.524620504384195 120 55 -9.285974017821546 120 56 -5.53257283254152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 69.0 5 18.0 6 26.0 7 21.0 8 64.0 9 75.0 10 69.0 11 49.0 12 40.0 13 56.0 14 63.0 15 81.0 16 114.0 17 150.0 18 200.0 19 257.0 20 312.0 21 366.0 22 474.0 23 597.0 24 677.0 25 725.0 26 716.0 27 847.0 28 917.0 29 869.0 30 1005.0 31 1111.0 32 1378.0 33 1670.0 34 2117.0 35 2326.0 36 2555.0 37 1983.0 38 936.0 39 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.07107366601858 0.5616763879887665 0.24195290559516094 0.12529704039749406 8 98.9198531000216 0.2635558435947289 0.6653704903866926 0.1512205659969756 9 97.5799481417459 1.5514261019878997 0.30682800345721695 0.5617977528089888 10 58.81183841002377 25.97105206308058 6.493843162670124 8.723266364225534 11 36.916695471828554 28.508468717594194 14.70359488420325 19.871240926374007 12 36.89781810326204 12.473536400950529 20.38885288399222 30.239792611795203 13 26.217325556275657 13.095701015338085 24.52797580470944 36.15899762367682 14 22.025327397674722 15.965769114405498 28.763452478713745 33.245451009206036 15 24.492621922194814 16.244753126487517 29.153143796789127 30.109481154528538 16 29.18449371191495 16.40520333635853 26.371061843640607 28.039241108085918 17 28.780740804771575 20.296494791891774 23.33924017806976 27.583524225266885 18 29.971916180600562 23.40030244113199 21.641823287967163 24.98595809030028 19 28.78192109925247 25.407250572527328 19.275806939463337 26.535021388756856 20 28.473873017245104 23.823313307688984 21.899987033755455 25.802826641310457 21 28.521431040442447 22.334082267542342 23.098859315589355 26.045627376425855 22 28.546122272629077 21.594296824368115 24.4199611147116 25.439619788291207 23 29.583063296608337 22.320155541153596 24.88658457550227 23.210196586735794 24 28.43810758263124 22.51890257074962 22.61395549794772 26.429034348671422 25 28.191834089436163 22.88183192914236 20.52711168718946 28.39922229423202 26 25.685850281263523 22.42752055387278 24.32280398096062 27.563825183903074 27 24.067430300410635 19.433758374756863 26.928895612708022 29.56991571212449 28 23.991516620498615 21.39023545706371 26.484591412742382 28.133656509695292 29 24.54616182572614 22.151625172890732 27.73167358229599 25.570539419087133 30 23.605947955390334 21.401400536007607 30.712371401400535 24.28028010720152 31 24.228254751699353 20.92912499458804 31.51924492358315 23.323375330129455 32 28.58377630839708 17.390552746445394 31.137905700332773 22.887765244824752 33 28.51391714644388 18.752434959525562 33.28860222501191 19.445045669018658 34 24.278856549755652 19.404921506724907 38.09626778532198 18.219954158197467 35 23.56145432536423 23.626302364791837 36.003631490207944 16.808611819635985 36 22.877909492082722 23.37976983646275 32.78532491130917 20.956995760145368 37 23.473893843622825 24.235864577019655 32.52662568187722 19.763615897480303 38 24.769570297286773 23.618503613310832 34.0559954995889 17.555930589813492 39 23.858660307825712 17.96228051159766 30.15824842835465 28.02081075222198 40 15.201146928490747 14.310539577721784 24.450430098184032 46.037883395603444 41 16.462750903470198 16.249401314930118 26.755780032220144 40.53206774937955 42 19.603428868300284 22.79851069356654 36.098363494674864 21.499696943458307 43 12.606244579358197 13.052905464006939 28.23937554206418 46.10147441457069 44 15.297438124185843 14.151107251411203 27.429439861050803 43.12201476335215 45 21.030321380682558 18.906527098922965 36.701414421038976 23.36173709935551 46 20.47046614200467 20.799100579434405 37.243794862924844 21.48663841563608 47 19.676736246164484 19.283460823717533 35.22624141060547 25.81356151951251 48 21.741573033707866 21.417458945548834 37.85220397579948 18.98876404494382 49 14.942329372994537 14.448009712947707 27.452085682074408 43.157575231983344 50 18.877727745064078 21.16383789400762 39.22757187391756 20.730862487010736 51 20.685768780614676 22.07291169968356 37.40951059863887 19.831808921062898 52 19.22309893349519 23.34605046388624 37.0545391485303 20.37631145408827 53 19.887445887445885 23.203463203463205 37.32900432900433 19.58008658008658 54 17.23541369317936 20.47921802690195 33.99074434496778 28.29462393495091 55 21.406621943302316 20.878597706124218 37.74940489071629 19.965375459857174 56 20.167958097052075 22.085623998961086 35.11969178823428 22.626726115752565 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.0 14 1.0 15 3.0 16 5.0 17 9.5 18 14.0 19 27.0 20 40.0 21 52.5 22 79.5 23 94.0 24 111.0 25 128.0 26 150.5 27 173.0 28 230.5 29 336.0 30 384.0 31 486.5 32 589.0 33 681.0 34 773.0 35 815.5 36 858.0 37 838.0 38 778.0 39 738.0 40 628.5 41 519.0 42 465.5 43 412.0 44 384.5 45 357.0 46 356.5 47 372.5 48 389.0 49 399.0 50 409.0 51 427.0 52 445.0 53 475.0 54 563.0 55 621.0 56 664.0 57 707.0 58 796.0 59 885.0 60 1003.5 61 1122.0 62 1253.0 63 1525.0 64 1666.0 65 1731.0 66 1796.0 67 1666.5 68 1537.0 69 1447.0 70 1357.0 71 1250.0 72 1031.0 73 919.0 74 772.5 75 626.0 76 531.5 77 437.0 78 352.5 79 229.5 80 191.0 81 133.5 82 76.0 83 53.0 84 30.0 85 21.0 86 12.0 87 8.0 88 3.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02160293799956794 10 0.0 11 0.004320587599913588 12 0.0 13 0.0 14 0.034564700799308706 15 0.15554115359688916 16 0.025923525599481533 17 0.034564700799308706 18 0.0 19 0.008641175199827177 20 0.034564700799308706 21 0.004320587599913588 22 0.0 23 0.0 24 0.0 25 0.0 26 0.1512205659969756 27 0.04320587599913588 28 0.1771440915964571 29 0.03888528839922229 30 0.047526463599049475 31 0.20738820479585227 32 0.025923525599481533 33 0.1901058543961979 34 0.09505292719809895 35 0.060488226398790236 36 0.13393821559732122 37 0.20306761719593863 38 0.15554115359688916 39 0.34564700799308706 40 0.5487146251890257 41 0.7690645927846187 42 0.20306761719593863 43 0.367249945992655 44 0.49686757399006265 45 0.11233527759775329 46 0.08209116439835817 47 0.025923525599481533 48 0.02160293799956794 49 0.3586087707928278 50 0.21170879239576584 51 0.3283646575934327 52 0.34132642039317346 53 0.19442644199611148 54 0.10369410239792613 55 0.17282350399654353 56 0.1901058543961979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 23145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.28731907539425 #Duplication Level Percentage of deduplicated Percentage of total 1 94.45853471790208 85.28407863469431 2 3.8905105996075995 7.025275437459494 3 0.7895870220605828 2.138690861957226 4 0.3206201847155094 1.1579174767768416 5 0.16748815619466909 0.756102829984878 6 0.11484902139063022 0.6221646143875567 7 0.06220988658659138 0.39317347159213656 8 0.0478537589127626 0.34564700799308706 9 0.01914150356510504 0.15554115359688916 >10 0.12441977317318276 1.8924173687621517 >50 0.00478537589127626 0.2289911427954202 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 53 0.2289911427954202 No Hit GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 45 0.19442644199611148 No Hit GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 37 0.15986174119680277 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 30 0.12961762799740764 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 28 0.12097645279758047 No Hit GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 27 0.1166558651976669 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.004320587599913588 0.0 0.0 0.0 0.0 14 0.008641175199827177 0.0 0.0 0.0 0.0 15 0.012961762799740765 0.0 0.0 0.0 0.0 16 0.017282350399654353 0.0 0.0 0.0 0.0 17 0.017282350399654353 0.0 0.0 0.0 0.0 18 0.02160293799956794 0.0 0.0 0.0 0.0 19 0.02160293799956794 0.0 0.0 0.0 0.0 20 0.02160293799956794 0.0 0.0 0.0 0.0 21 0.030244113199395118 0.0 0.0 0.0 0.0 22 0.030244113199395118 0.0 0.0 0.0 0.0 23 0.034564700799308706 0.0 0.0 0.0 0.0 24 0.034564700799308706 0.0 0.0 0.0 0.0 25 0.03888528839922229 0.0 0.0 0.0 0.0 26 0.05184705119896306 0.0 0.0 0.0 0.0 27 0.056167638798876644 0.0 0.0 0.0 0.0 28 0.060488226398790236 0.0 0.0 0.0 0.0 29 0.060488226398790236 0.0 0.0 0.0 0.0 30 0.060488226398790236 0.0 0.0 0.0 0.0 31 0.060488226398790236 0.0 0.0 0.0 0.0 32 0.060488226398790236 0.0 0.0 0.0 0.0 33 0.060488226398790236 0.0 0.0 0.0 0.0 34 0.060488226398790236 0.0 0.0 0.0 0.0 35 0.060488226398790236 0.0 0.0 0.0 0.0 36 0.060488226398790236 0.0 0.0 0.0 0.0 37 0.060488226398790236 0.0 0.0 0.0 0.0 38 0.060488226398790236 0.0 0.0 0.0 0.0 39 0.060488226398790236 0.0 0.0 0.0 0.0 40 0.060488226398790236 0.0 0.0 0.0 0.0 41 0.060488226398790236 0.0 0.0 0.0 0.0 42 0.06480881399870382 0.0 0.0 0.0 0.0 43 0.06912940159861741 0.0 0.0 0.0 0.0 44 0.073449989198531 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTGC 15 0.00795194 50.584614 37 AACCAAC 20 3.911252E-4 50.473682 41 CTTGCCT 15 0.008021477 50.473682 41 CGTCCTG 15 0.008091465 50.36324 36 TGCAATC 15 0.008232801 50.14379 50 ACCGCTG 15 0.008232801 50.14379 42 ACCAACA 20 4.0404464E-4 50.14379 42 TTGCCTA 15 0.008232801 50.14379 42 CAAGCGC 15 0.008232801 50.14379 50 CGCTGCA 15 0.0083041545 50.034782 44 CCAACAC 20 4.0842564E-4 50.034782 43 CCTGCGC 15 0.0083041545 50.034782 43 GTTCCTG 15 0.0083041545 50.034782 11 CCAAAAC 20 4.0842564E-4 50.034782 43 AAGAGTG 15 0.0083041545 50.034782 10 CAACACA 20 4.0842564E-4 50.034782 44 TTCCTGC 15 0.0083041545 50.034782 12 TGCCTAG 15 0.0083041545 50.034782 43 GTAAGAC 20 4.0842564E-4 50.034782 34 TCCTGCT 15 0.0083041545 50.034782 13 >>END_MODULE