FastQCFastQC Report
Fri 17 Jun 2016
SRR1526276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18416
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA950.5158557775847089No Hit
AAGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA550.29865334491746304No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA340.18462206776715898No Hit
AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA300.1629018245004344No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC290.15747176368375326No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG290.15747176368375326No Hit
AAGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA280.1520417028670721No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG280.1520417028670721No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG280.1520417028670721No Hit
AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA260.14118158123370983No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG240.13032145960034752No Hit
AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACAAAAAAA230.12489139878366637No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA190.10317115551694178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCT150.00782341350.75000439
GCACCGA150.00782341350.75000440
ACCAACC203.7856345E-450.7538
ACAAACC251.8368171E-550.74999638
CAACCAA251.8368171E-550.74999640
CAAACCA251.8368171E-550.74999639
TATGCTT150.00791003450.6094235
ATGCTTG150.00791003450.6094236
CATATGC251.9617935E-550.1923133
CCTCGCT150.00817417850.19230744
GGTTGAT251.994114E-550.05479415
CCAACAC451.200533E-1050.05479443
TTCGTGG204.053794E-450.05479415
AATTCGT150.00826366750.05479413
GTACCTG251.994114E-550.0547949
AATTCCT150.00826366750.05479413
GGACCCA150.00826366750.0547949
CTCTTTC150.00826366750.05479411
CATTTGA150.00826366750.05479446
ACCTGGT251.994114E-550.05479411
Produced by FastQC (version 0.11.5)