##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526276_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18416 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.17864900086881 39.0 38.0 39.0 33.0 40.0 2 36.95183536055604 39.0 38.0 39.0 33.0 40.0 3 35.79593831450912 39.0 36.0 39.0 29.0 40.0 4 35.91203301476977 39.0 36.0 39.0 29.0 40.0 5 36.15969808861859 39.0 37.0 39.0 30.0 40.0 6 36.248425282363165 38.0 36.0 39.0 31.0 40.0 7 36.39715464813206 38.0 37.0 39.0 32.0 40.0 8 35.38211337966985 38.0 35.0 39.0 29.0 40.0 9 35.38461120764553 38.0 35.0 39.0 29.0 40.0 10 34.94624239791486 38.0 35.0 39.0 27.0 40.0 11 33.515529973935706 35.0 31.0 39.0 24.0 40.0 12 32.09117072111208 35.0 29.0 39.0 16.0 40.0 13 32.054409209383145 36.0 29.0 39.0 14.0 40.0 14 31.91463944396177 36.0 29.0 39.0 14.0 40.0 15 32.21866854908775 36.0 30.0 39.0 15.0 40.0 16 32.24945699391833 36.0 30.0 39.0 16.0 40.0 17 32.29430929626412 36.0 30.0 39.0 17.0 40.0 18 32.38064726324935 36.0 30.0 39.0 18.0 40.0 19 33.30180278019114 36.0 31.0 39.0 23.0 40.0 20 33.298490443092966 36.0 31.0 39.0 23.0 40.0 21 34.87586880973067 38.0 35.0 39.0 27.0 40.0 22 34.52193744569939 38.0 34.0 39.0 25.0 40.0 23 34.77742180712424 38.0 35.0 39.0 27.0 40.0 24 34.28274326672459 38.0 33.0 39.0 24.0 40.0 25 34.59165942658558 38.0 34.0 39.0 27.0 40.0 26 34.82504344048653 38.0 35.0 39.0 27.0 40.0 27 34.73349261511729 38.0 35.0 39.0 27.0 40.0 28 34.63852085143353 38.0 35.0 39.0 27.0 40.0 29 34.41746307558645 38.0 34.0 39.0 26.0 40.0 30 34.318364465682016 38.0 34.0 39.0 25.0 40.0 31 33.69531928757602 38.0 33.0 39.0 22.0 40.0 32 32.72279539530843 37.0 32.0 39.0 14.0 40.0 33 33.827052562988705 38.0 33.0 39.0 23.0 40.0 34 33.500217202432665 38.0 33.0 39.0 21.0 40.0 35 33.53372067767159 38.0 33.0 39.0 22.0 40.0 36 31.45330147697654 35.0 30.0 38.0 10.0 39.0 37 31.084328844483057 35.0 30.0 38.0 4.0 39.0 38 30.664205039096437 35.0 30.0 38.0 4.0 39.0 39 28.79306038227628 33.0 26.0 38.0 4.0 39.0 40 25.159698088618594 31.0 11.0 37.0 4.0 39.0 41 22.044798001737618 26.0 9.0 33.0 4.0 38.0 42 25.476433536055605 30.0 20.0 34.0 4.0 38.0 43 22.068690269331018 26.0 8.0 33.0 4.0 37.0 44 23.094645960034754 28.0 10.0 33.0 4.0 37.0 45 25.138086446568202 30.0 19.0 35.0 4.0 38.0 46 25.150304083405736 30.0 12.0 36.0 4.0 38.0 47 24.43728279756733 30.0 7.0 35.0 4.0 38.0 48 24.217256733275413 30.0 6.0 35.0 4.0 38.0 49 22.00510425716768 27.0 4.0 35.0 4.0 38.0 50 24.138900955690705 30.0 4.0 35.0 4.0 38.0 51 25.527530408340574 32.0 4.0 38.0 4.0 39.0 52 25.354474370112946 32.0 4.0 38.0 4.0 40.0 53 24.878475238922675 31.0 4.0 37.0 4.0 39.0 54 24.14541702867072 31.0 4.0 37.0 4.0 39.0 55 24.328953084274545 31.0 4.0 37.0 4.0 39.0 56 24.254561251086013 32.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.9698686272354422 1 2 2.271282572925223 1 3 3.6082226742548116 1 4 2.547392446980453 1 5 2.3554647178253845 1 6 3.3904301375182513 1 7 1.6775162625534463 1 8 2.7554678177940772 1 9 2.029128973816512 1 10 3.0040473709226347 1 11 -3.4279764141639077 1 12 -5.2419947679136065 1 13 -20.19772438713948 1 14 -16.954165910537117 1 15 -8.869328293424175 1 16 -18.877382824418707 1 17 -19.195549254590453 1 18 -17.061116488148187 1 19 -6.605930839484714 1 20 -8.850324475805465 1 21 -6.385372637246377 1 22 -1.599736867702049 1 23 -4.819013573897145 1 24 -1.4690147963008862 1 25 -2.3847372307060013 1 26 1.0354780548674967 1 27 0.1544541413478271 1 28 0.8134062864691813 1 29 0.7689223182215841 1 30 1.3654369876079429 1 31 1.767930786109659 1 32 -11.948207099357518 1 33 -0.25426866420749406 1 34 -0.0366595125838316 1 35 0.8031183150345989 1 36 3.237819140052288 1 37 4.522236693243279 1 38 4.871228767560179 1 39 4.373823469862746 1 40 7.759100215163791 1 41 -5.0088557148727375 1 42 -10.157262092965821 1 43 -12.432756758109797 1 44 -16.04124973747447 1 45 -12.384847246843698 1 46 -17.170779891392126 1 47 -17.14324682668544 1 48 -16.06651464762617 1 49 -14.046571127279964 1 50 -15.397649596913233 1 51 -15.938151747454171 1 52 -16.312786524987736 1 53 -16.103014021214474 1 54 -16.6969711321685 1 55 -15.472090290598835 1 56 -14.898702026101127 2 1 2.094868627235442 2 2 2.0555962984154164 2 3 3.9121442428822633 2 4 2.407686564627511 2 5 3.6495823648842105 2 6 3.4320968041849156 2 7 3.2485946939259946 2 8 3.1525266413234903 2 9 2.7889328953851447 2 10 2.7270865866089125 2 11 -3.8225842573011626 2 12 -10.697877120854784 2 13 -13.744293014590458 2 14 -15.329165910537117 2 15 -6.384034175777117 2 16 -4.561206353830471 2 17 -2.2029021957669244 2 18 -10.438567468540345 2 19 -0.596126917916088 2 20 -2.838069573844681 2 21 2.96756853922421 2 22 3.7335964656312868 2 23 2.7373589751224614 2 24 2.305495007620685 2 25 2.9191843379214504 2 26 4.275674133298864 2 27 3.60298355311253 2 28 4.114876874704478 2 29 4.264020357437275 2 30 4.740436987607943 2 31 -0.22716725310602826 2 32 -9.737422785632027 2 33 -0.7273078798937682 2 34 -0.18371833611324462 2 35 0.11684380523067972 2 36 6.063799532209153 2 37 5.098217085400144 2 38 7.290346414619005 2 39 8.297843077705881 2 40 8.310570803399084 2 41 6.280359971401772 2 42 3.23979673056359 2 43 0.6603804967921647 2 44 -10.850073266886234 2 45 1.0906429492347343 2 46 -11.001662244333303 2 47 -10.91040368943054 2 48 -9.84102445154774 2 49 -11.235296617476044 2 50 -9.608433910638722 2 51 -11.20530861019927 2 52 -10.46719828969362 2 53 -9.20595519768506 2 54 -14.412657406678303 2 55 -9.116698133736088 2 56 -8.957525555512891 3 1 1.1886186272354422 3 2 2.701429631748752 3 3 4.6725609095489276 3 4 4.032686564627511 3 5 3.5558323648842105 3 6 3.0779301375182513 3 7 3.4256780272593303 3 8 4.34002664132349 3 9 4.455599562051809 3 10 4.393753253275577 3 11 2.2503324093655053 3 12 2.802122879145216 3 13 -1.0047096812571255 3 14 -3.2041659105371174 3 15 -0.7277841757771171 3 16 4.907543646169529 3 17 4.453347804233076 3 18 4.144765864792987 3 19 2.57053974875058 3 20 2.839013759488651 3 21 3.74881853922421 3 22 4.775263132297951 3 23 3.695692308455797 3 24 4.128411674287349 3 25 4.335851004588115 3 26 3.6090074666321996 3 27 4.41548355311253 3 28 4.927376874704478 3 29 3.993187024103939 3 30 2.740436987607943 3 31 4.731166080227304 3 32 3.8146605477013047 3 33 4.668525453439564 3 34 4.535031663886755 3 35 4.356427138564012 3 36 6.636716198875817 3 37 7.827383752066808 3 38 7.915346414619005 3 39 9.224926411039217 3 40 11.623070803399084 3 41 8.72827663806844 3 42 7.80229673056359 3 43 0.5562138301254969 3 44 1.962426733113766 3 45 7.642726282568066 3 46 1.8420877556666966 3 47 -3.358320356097206 3 48 -0.5076911182144066 3 49 -9.537379950809378 3 50 0.6832327560279445 3 51 -1.4032252768659355 3 52 -1.46719828969362 3 53 -2.2059551976850607 3 54 -2.8293240733449707 3 55 -2.1375314670694223 3 56 0.9799744444871088 4 1 1.9032019605687722 4 2 2.2222629650820878 4 3 3.7121442428822604 4 4 3.0493532312941767 4 5 4.282915698217543 4 6 3.5570968041849156 4 7 2.1402613605926604 4 8 3.035859974656823 4 9 2.705599562051809 4 10 2.7687532532755768 4 11 4.119082409365504 4 12 3.418789545811883 4 13 4.247373652076206 4 14 1.6791674227962154 4 15 6.065965824222886 4 16 6.430460312836196 4 17 5.855431137566406 4 18 5.611432531459656 4 19 4.270539748750583 4 20 4.753597092821984 4 21 3.53423520589088 4 22 3.8002631322979497 4 23 3.445692308455797 4 24 3.4054950076206794 4 25 2.0525176712547832 4 26 2.325674133298868 4 27 2.3779835531125286 4 28 2.256543541371144 4 29 3.380687024103942 4 30 1.1987703209412786 4 31 3.656166080227301 4 32 3.379243881034636 4 33 2.614358786772897 4 34 3.582948330553421 4 35 2.4168438052306804 4 36 4.688799532209153 4 37 3.839883752066811 4 38 4.098679747952335 4 39 6.422843077705881 4 40 6.2689041367324165 4 41 4.263693304735106 4 42 6.589796730563592 4 43 6.777047163458832 4 44 8.066593399780434 4 45 8.340642949234734 4 46 6.490004422333364 4 47 4.664596310569461 4 48 5.925642215118927 4 49 2.4730367158572903 4 50 7.2748994226946095 4 51 5.4696913898007296 4 52 6.391135043639714 4 53 5.144044802314941 4 54 1.3873425933216943 4 55 3.50830186626391 4 56 4.409141111153776 5 1 3.163417014332218 5 2 3.5502199543293997 5 3 4.91752058696828 5 4 5.008493016240415 5 5 4.77861462294873 5 6 4.229139814937604 5 7 4.33596028532385 5 8 4.273494383258971 5 9 4.270115691084065 5 10 4.284882285533641 5 11 5.606179183559057 5 12 7.439219653338768 5 13 8.536620963904163 5 14 7.5297050572048185 5 15 8.656288404868043 5 16 8.35411622681469 5 17 8.276936513910492 5 18 7.709281993825243 5 19 6.376991361653804 5 20 7.136392791746715 5 21 5.451439506966146 5 22 6.303488938749567 5 23 5.623111663294509 5 24 5.633451996867997 5 25 5.182625198136499 5 26 3.7944913375999434 5 27 3.429596456338338 5 28 5.118909132768998 5 29 3.438751540232971 5 30 4.365436987607943 5 31 4.614230596356336 5 32 5.24913635415292 5 33 4.013283517955692 5 34 3.703378438080307 5 35 3.19211262243498 5 36 5.000627489198401 5 37 4.123754719808744 5 38 4.907281898489973 5 39 5.159402217490829 5 40 2.7420224163023086 5 41 7.793800831616828 5 42 9.812377375724878 5 43 11.130810604319045 5 44 10.99670092666215 5 45 8.157847250310002 5 46 8.574950658892504 5 47 9.443090934225374 5 48 7.960050817269465 5 49 10.75798295241643 5 50 8.921135981834396 5 51 8.099798916682452 5 52 7.577156549016056 5 53 8.350496415218164 5 54 7.646482378267933 5 55 8.626581436156386 5 56 7.848926057390333 6 1 3.038834144476823 6 2 3.216515838645307 6 3 4.63053504747996 6 4 4.4723417370413046 6 5 4.166823744194552 6 6 4.149050827173426 6 7 4.103479751397259 6 8 4.574940434426935 6 9 4.481461631017325 6 10 5.251511873965235 6 11 5.1501168921241245 6 12 6.7762608101797 6 13 8.04162652563943 6 14 8.49410995153185 6 15 7.848724444912541 6 16 8.856897094445394 6 17 8.12899435595721 6 18 7.575800347551606 6 19 6.880884576336786 6 20 6.413367207764516 6 21 5.519292677155242 6 22 5.917504511608293 6 23 4.8077612739730355 6 24 5.782506501873556 6 25 5.120333763208805 6 26 4.92797298387358 6 27 5.219362863457356 6 28 3.9209113574630976 6 29 5.525514610310836 6 30 5.193023194504498 6 31 6.080304011261788 6 32 7.035565720115098 6 33 6.015508212060254 6 34 5.385247181128136 6 35 6.208797828219186 6 36 6.872707578186159 6 37 6.15712513137715 6 38 7.751553311170728 6 39 8.221693652418526 6 40 4.901088044778394 6 41 6.007371465654646 6 42 9.558762247804971 6 43 11.571300037022048 6 44 12.417168112424108 6 45 9.846390075671511 6 46 11.640579134977038 6 47 12.211722747351072 6 48 11.923343364544213 6 49 12.168439014707864 6 50 11.756508618096912 6 51 12.727162654168549 6 52 13.295732744789142 6 53 13.268182733349423 6 54 12.627572478379165 6 55 12.174968532930578 6 56 11.525233065176767 7 1 1.2098686272354442 7 2 2.0689296317487518 7 3 2.678810909548929 7 4 0.3826865646275124 7 5 1.9295823648842116 7 6 1.5504301375182479 7 7 0.5269280272593306 7 8 1.8825266413234871 7 9 0.8055995620518104 7 10 1.7287532532755776 7 11 1.0990824093655078 7 12 3.6921228791452165 7 13 5.174040318742875 7 14 3.925834089462885 7 15 4.925965824222885 7 16 6.383793646169529 7 17 5.442097804233079 7 18 5.204765864792989 7 19 4.2105397487505805 7 20 2.100263759488648 7 21 2.0475685392242085 7 22 2.2002631322979482 7 23 1.425692308455794 7 24 2.2321616742873474 7 25 1.405851004588115 7 26 1.219007466632199 7 27 -0.10201644688746825 7 28 1.5498768747044807 7 29 -0.339312975896064 7 30 -0.47456301239206056 7 31 1.4361660802273022 7 32 2.7459105477013033 7 33 0.02102545343956308 7 34 1.996281663886755 7 35 -0.7898228614359866 7 36 -2.764533801124184 7 37 -2.0401162479331916 7 38 -3.154653585380995 7 39 -0.06382358896078344 7 40 -6.584429196600915 7 41 -10.14297336193156 7 42 0.8897967305635888 7 43 3.2237138301254973 7 44 3.2999267331137645 7 45 -0.1260237174319343 7 46 1.5233377556666952 7 47 2.757929643902795 7 48 0.632308881785594 7 49 4.1863700491906215 7 50 2.1082327560279452 7 51 4.043024723134064 7 52 3.037801710306379 7 53 3.264044802314938 7 54 5.140675926655028 7 55 3.0549685329305767 7 56 3.5224744444871092 8 1 2.3247073369128586 8 2 2.4534457607810083 8 3 4.014294780516671 8 4 3.7181704355952547 8 5 3.8431307519809863 8 6 3.3904301375182513 8 7 2.3037022208077147 8 8 3.4025266413234903 8 9 3.0443092394711613 8 10 3.381656479082025 8 11 5.735211441623569 8 12 7.084380943661344 8 13 8.27855644777513 8 14 7.432930863656434 8 15 7.688546469384171 8 16 7.450890420363081 8 17 7.405968771975012 8 18 6.870572316405891 8 19 5.4737655552021955 8 20 5.910586340133811 8 21 4.451439506966146 8 22 5.561553454878599 8 23 4.494079405229989 8 24 4.633451996867997 8 25 2.5697219723300506 8 26 3.0202977892128473 8 27 3.8812093595641457 8 28 2.7963284876077026 8 29 3.696816056362003 8 30 2.5589853747047187 8 31 3.9690693060337523 8 32 4.474942805765824 8 33 2.3681222276331155 8 34 2.445313921951275 8 35 1.966306170822076 8 36 0.06514361823065684 8 37 -0.48914850599770787 8 38 -0.06046003699389857 8 39 1.9658538303940567 8 40 -2.3224937127299476 8 41 -10.367489490963818 8 42 3.231732214434558 8 43 7.034036410770657 8 44 5.48057189440409 8 45 2.6417182180519383 8 46 4.574950658892504 8 47 5.57212319228989 8 48 5.960050817269465 8 49 7.112821662093847 8 50 4.469523078608589 8 51 6.648186013456645 8 52 5.609414613532188 8 53 7.544044802314939 8 54 7.710998507300189 8 55 6.8846459522854175 8 56 6.848926057390333 9 1 2.7912971986640116 9 2 2.9246439174630368 9 3 4.150239480977497 9 4 3.9612579931989416 9 5 2.97101093631278 9 6 2.9975729946611054 9 7 3.5283565986878997 9 8 3.5096694984663444 9 9 3.9913138477660937 9 10 3.6616103961327227 9 11 4.25479669507979 9 12 7.552122879145216 9 13 8.285468890171444 9 14 7.902976946605737 9 15 7.830251538508598 9 16 7.135222217598098 9 17 7.600669232804506 9 18 8.001908721935841 9 19 5.891968320179149 9 20 5.477406616631505 9 21 4.860425682081356 9 22 3.5788345608693817 9 23 3.9099780227415124 9 24 4.222161674287349 9 25 3.5144224331595453 9 26 2.2161503237750537 9 27 4.0494121245410994 9 28 3.0970197318473325 9 29 3.6092584526753697 9 30 3.365436987607943 9 31 3.6775946516558733 9 32 5.953053404844159 9 33 4.338168310582418 9 34 4.494853092458186 9 35 3.5216057099925813 9 36 2.8197519131615323 9 37 3.7470266092096622 9 38 3.5582035574761512 9 39 5.970462125324932 9 40 0.4355708033990844 9 41 -3.4101162190744176 9 42 4.23979673056359 9 43 8.922285258696924 9 44 7.364212447399481 9 45 5.138261996853782 9 46 8.123337755666697 9 47 7.155072501045652 9 48 6.813737453214166 9 49 8.199227192047765 9 50 5.415375613170802 9 51 6.3244532945626375 9 52 6.800658853163522 9 53 7.044044802314939 9 54 7.420675926655029 9 55 6.81782567578772 9 56 5.721045873058536 10 1 2.969868627235442 10 2 3.588929631748755 10 3 4.838810909548926 10 4 4.582686564627508 10 5 4.769582364884208 10 6 4.550430137518248 10 7 4.0069280272593275 10 8 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15 -4.692857705188882 115 16 -7.5244416479481195 115 17 -8.01907866635516 115 18 -1.6493517822658355 115 19 -3.7235778983082426 115 20 -1.3209127110995844 115 21 -0.6794902843052029 115 22 0.10614548523912504 115 23 0.5927511319852101 115 24 1.7662793213461754 115 25 0.43879218105870166 115 26 -0.6115807686619164 115 27 -0.9631929174757019 115 28 -0.5983584194131666 115 29 -2.172254152366648 115 30 -0.5169159535685282 115 31 -1.879128037419754 115 32 -6.418795334651637 115 33 -0.37191572303102305 115 34 -1.2719536302308931 115 35 -0.6674699202595171 115 36 0.473113257699346 115 37 1.4634131638315147 115 38 1.6359346499131213 115 39 1.9032352345686299 115 40 4.994394332810849 115 41 4.814673696891969 115 42 2.372149671740061 115 43 2.096655006596084 115 44 0.1940443801725884 115 45 -0.6789648939025206 115 46 -2.9943093031568324 115 47 -3.7903056502148544 115 48 -6.772397000567349 115 49 -5.987747597868202 115 50 -2.397649596913233 115 51 -0.9969752768659355 115 52 -2.7833747602818555 115 53 -2.632425785920354 115 54 -2.9322652498155577 115 55 -1.8838549964811868 115 56 -3.722231437865833 116 1 1.2639862742942682 116 2 1.506576690572281 116 3 2.0199873801371595 116 4 1.959157152862808 116 5 1.0613470707665655 116 6 2.2139595492829542 116 7 2.4422221449063883 116 8 2.108408994264664 116 9 2.499717209110635 116 10 1.3569885473932217 116 11 -2.4279764141639077 116 12 -4.4184653561489 116 13 -6.197724387139477 116 14 -11.365930616419469 116 15 -10.575210646365353 116 16 -6.053853412654 116 17 -8.842608078119866 116 18 -4.237587076383484 116 19 -4.076519074778833 116 20 -5.144442122864291 116 21 1.4381567745183261 116 22 3.106145485239125 116 23 3.180986426102855 116 24 0.4133381448755813 116 25 2.0858510045881147 116 26 3.3295957019263156 116 27 2.742689435465472 116 28 3.1663474629397754 116 29 1.7100987888098231 116 30 3.600731105255001 116 31 -0.8203045080079896 116 32 -7.71291298171046 116 33 2.157496041674861 116 34 0.7868698991808714 116 35 -1.4321758026124591 116 36 0.884877963581701 116 37 2.5810602226550436 116 38 2.341817002854299 116 39 3.5502940580980393 116 40 6.347335509281436 116 41 3.1087913439507915 116 42 1.7250908482106482 116 43 -11.07981558163921 116 44 -3.8059556198274116 116 45 0.3798586355092439 116 46 -10.288426950215657 116 47 -12.672658591391324 116 48 -11.30180876527323 116 49 -9.987747597868202 116 50 -7.868237832207349 116 51 -4.5263870415718195 116 52 -4.312786524987736 116 53 -6.220661080038003 116 54 -5.402853485109677 116 55 -4.766207937657658 116 56 -3.839878496689362 117 1 1.414313071679885 117 2 1.833374076193195 117 3 3.745477576215592 117 4 3.227131009071954 117 5 3.316249031550875 117 6 2.9459856930738084 117 7 -0.015294194962891083 117 8 2.4025266413234903 117 9 0.7611551176073661 117 10 2.1020865866089125 117 11 -5.447584257301163 117 12 -3.392321565299227 117 13 -5.230404125701568 117 14 -8.065277021648228 117 15 -4.411811953554896 117 16 -5.380650798274914 117 17 -2.5223466402113672 117 18 -0.5774563574292344 117 19 0.23720641541724774 117 20 -0.2686251294002382 117 21 3.1897907614464316 117 22 3.844707576742394 117 23 3.0568034195669114 117 24 -6.305616103490429 117 25 2.641406560143672 117 26 0.41456302218775676 117 27 3.6446502197791943 117 28 2.878765763593364 117 29 3.625131468548382 117 30 3.2543258764968286 117 31 -0.08827836421713897 117 32 -3.2096450078542524 117 33 0.4254698978840068 117 34 0.7607261083311982 117 35 -0.8831561947693203 117 36 2.022132865542485 117 37 2.1954393076223653 117 38 0.6097908590634482 117 39 4.922843077705885 117 40 4.4911263589546415 117 41 2.363693304735108 117 42 0.26757450834136876 117 43 -1.922952836541171 117 44 -1.544517711330677 117 45 0.22953183812362354 117 46 -2.987773355444414 117 47 -5.535403689430538 117 48 -8.785468895992185 117 49 -5.6936299508093775 117 50 -2.469545021749834 117 51 -2.3303086101992676 117 52 0.26891282141749073 117 53 -1.2337329754628392 117 54 -6.023768517789415 117 55 -5.49169813373609 117 56 -1.068636666624002 118 1 1.354484011850829 118 2 1.6966219394410587 118 3 3.232657063395081 118 4 2.9365327184736643 118 5 2.957274672576517 118 6 2.621199368287485 118 7 2.6684664887977902 118 8 2.710218949015797 118 9 1.8979072543595024 118 10 2.38413786866019 118 11 -7.550148359865265 118 12 -7.563261736239401 118 13 -10.785959681257125 118 14 -1.6464736028448108 118 15 -17.634034175777117 118 16 -6.551590969215088 118 17 -8.154825272690001 118 18 -1.355234135207013 118 19 -3.275614097403267 118 20 -3.6874285482036555 118 21 3.1214146930703635 118 22 3.861801593836411 118 23 3.0226153853788773 118 24 -0.14322294109726386 118 25 3.162774081511195 118 26 4.243622851247586 118 27 4.362598937727917 118 28 2.759107643935245 118 29 4.488379331796246 118 30 4.75005237222333 118 31 2.971550695611917 118 32 -12.269474067683312 118 33 2.981025453439564 118 34 3.085512433117522 118 35 2.527100215487092 118 36 6.278543121952737 118 37 6.562960675143728 118 38 6.011500260772852 118 39 8.87156102642383 118 40 11.166340034168318 118 41 12.1585650996069 118 42 7.95133519210205 118 43 1.5898676762793436 118 44 6.2076190408060725 118 45 3.8278224364142197 118 46 -5.18435455202561 118 47 -6.971301125327976 118 48 -10.469229579752868 118 49 -2.539783796963224 118 50 -2.999459551664362 118 51 -1.9969752768659355 118 52 -1.9191213666166966 118 53 -4.225185966915831 118 54 -3.733170227191124 118 55 -4.286569928607882 118 56 -0.03444863243596785 119 1 1.803201960568778 119 2 2.2222629650820878 119 3 3.5788109095489276 119 4 3.3660198979608467 119 5 3.482915698217546 119 6 2.8070968041849156 119 7 2.6235946939259946 119 8 2.8191933079901546 119 9 2.705599562051809 119 10 2.2687532532755768 119 11 -7.614250923967827 119 12 -8.697877120854784 119 13 -0.9526263479237933 119 14 -0.37083257720378526 119 15 -6.800700842443785 119 16 -8.019539687163803 119 17 -1.9112355291002565 119 18 1.144765864792987 119 19 -0.34612691791608796 119 20 0.20359709282198324 119 21 2.71756853922421 119 22 2.4835964656312868 119 23 1.8623589751224614 119 24 -0.44450499237931496 119 25 2.4191843379214504 119 26 2.9423407999655353 119 27 2.8946502197791943 119 28 3.2398768747044784 119 29 2.930687024103939 119 30 3.1987703209412786 119 31 1.356166080227304 119 32 -5.154089452298695 119 33 1.981025453439564 119 34 1.7329483305534197 119 35 2.866843805230676 119 36 6.688799532209153 119 37 6.723217085400144 119 38 7.248679747952341 119 39 9.006176411039217 119 40 8.185570803399084 119 41 5.030359971401772 119 42 3.656463397230258 119 43 2.6603804967921647 119 44 -1.850073266886234 119 45 -3.5760237174319336 119 46 -6.126662244333303 119 47 -6.035403689430538 119 48 -7.3410244515477405 119 49 -6.9436299508093775 119 50 -7.525100577305389 119 51 -12.83030861019927 119 52 -13.34219828969362 119 53 -13.95595519768506 119 54 -10.57932407334497 119 55 -15.24169813373609 119 56 -13.374192222179557 120 1 2.1841543415211575 120 2 2.6032153460344674 120 3 1.9359537666917817 120 4 1.639829421770365 120 5 1.5067252220270646 120 6 1.0332872803753972 120 7 0.8497851701164763 120 8 1.1168123556092056 120 9 0.9198852763375243 120 10 0.9116103961327227 120 11 -9.209489019205922 120 12 -5.447877120854784 120 13 -6.07167396697141 120 14 -6.239880196251402 120 15 -5.20546274720569 120 16 -7.936206353830471 120 17 -3.7207593386240667 120 18 -3.6409484209212977 120 19 -3.0723173941065625 120 20 -3.7368790976542066 120 21 0.18185425350992546 120 22 1.6859774180122358 120 23 0.588549451312943 120 24 -1.0992668971412236 120 25 -0.057006138269031226 120 26 1.930436038060769 120 27 2.120840695969676 120 28 2.2041625889901937 120 29 2.466401309818224 120 30 -0.34884872667777245 120 31 -6.929548205486981 120 32 -12.154089452298695 120 33 -8.661831689417578 120 34 -9.89800405039896 120 35 -6.049822861435988 120 36 -3.715962372552756 120 37 -4.931544819361765 120 38 -3.2632250139524217 120 39 -2.67239501753221 120 40 -6.135857768029489 120 41 -5.945830504788702 120 42 -8.01020326943641 120 43 -7.613429027017361 120 44 -7.100073266886234 120 45 -8.826023717431934 120 46 -13.590947958619017 120 47 -14.416356070382921 120 48 -12.150548261071549 120 49 -15.193629950809378 120 50 -11.120338672543484 120 51 -11.782689562580222 120 52 -11.84219828969362 120 53 -11.38452662625649 120 54 -11.150752644773542 120 55 -12.68217432421228 120 56 -8.671811269798605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 461.0 5 37.0 6 12.0 7 18.0 8 46.0 9 40.0 10 35.0 11 30.0 12 34.0 13 47.0 14 62.0 15 87.0 16 110.0 17 122.0 18 177.0 19 180.0 20 233.0 21 329.0 22 378.0 23 409.0 24 463.0 25 501.0 26 499.0 27 522.0 28 564.0 29 599.0 30 727.0 31 856.0 32 1103.0 33 1339.0 34 1524.0 35 1749.0 36 1918.0 37 1724.0 38 1021.0 39 459.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.97371850564727 0.961120764552563 0.04887054735013033 0.01629018245004344 8 98.50673327541269 1.1348827106863597 0.1954821894005213 0.1629018245004344 9 93.2341442224153 5.082536924413553 0.9176802780191139 0.7656385751520417 10 55.39748045178106 25.27693310165074 11.082754126846222 8.24283231972198 11 36.7235013032146 27.057993049522157 18.17984361424848 18.03866203301477 12 27.492397914856646 20.536490008688098 21.090356211989576 30.88075586446568 13 25.352953953084274 17.99522154648132 23.034317984361426 33.61750651607298 14 17.962087882244308 23.605453261637066 24.490793547335834 33.941665308782795 15 21.395146370660573 24.458591794536947 25.623027532919796 28.523234301882688 16 28.973384030418252 26.442151004888647 19.97827267789245 24.606192286800653 17 25.152074733869217 30.2954594829459 20.274820769063652 24.277645014121223 18 23.05729025251154 36.54086342655444 19.630735813195766 20.771110507738257 19 25.2877932232841 34.0139009556907 17.843179843614248 22.855125977410946 20 22.54928583066312 37.07163417150926 20.127084125346222 20.251995872481398 21 22.41351219247271 33.93797860207462 21.989898441318633 21.658610764134036 22 21.99837089329351 37.588922074395875 20.56475699158295 19.847950040727667 23 20.987185056472633 39.51998262380539 20.047784535186793 19.445047784535184 24 21.06320590790617 39.454821894005214 19.18983492615117 20.292137271937445 25 23.837966985230235 38.11902693310165 18.538227628149436 19.50477845351868 26 21.90745473329346 37.28454135174813 19.090859659616115 21.717144255342287 27 19.530740821203562 38.23050184662177 19.487290897240932 22.75146643493374 28 19.096297101843295 41.074438584090046 19.150671524115058 20.678592789951608 29 20.59095106186519 37.28205963825973 23.176361957525394 18.950627342349684 30 20.2845815456471 36.01803073915168 26.70395915928963 16.993428555911585 31 23.717565141707013 36.35424033074035 22.694881140183863 17.233313387368764 32 24.28043879656783 35.065710872162484 20.267188009123494 20.386662322146194 33 22.289287656334963 33.59978249048396 22.191408374116367 21.91952147906471 34 22.752702188908806 37.569931019499215 23.00255282168269 16.674813969909295 35 21.55968285000543 37.41175192788096 25.708699902248288 15.31986531986532 36 22.59134406263593 38.739669421487605 21.520226185297954 17.148760330578515 37 20.125136017410227 37.49727965179543 26.501632208922743 15.8759521218716 38 22.78088818829157 40.550089688536175 21.970973528292657 14.698048594879602 39 18.727183566719336 30.61079932436114 19.173977006483952 31.488040102435573 40 14.135067205769861 21.28182712271883 17.812260955086874 46.770844716424435 41 14.615047639908004 23.787098893877996 20.10185083780528 41.49600262840872 42 19.647519582245433 38.642297650130544 23.689077458659703 18.021105308964316 43 11.235281290885304 19.390536415176623 19.42324465765373 49.950937636284344 44 13.029244871235267 23.826931470973374 17.4814491488433 45.662374508948055 45 21.756617207456927 34.75732376759606 26.10467960215229 17.38137942279472 46 18.035102972341466 35.342063793946636 26.120741183502687 20.502092050209207 47 15.968486824232544 32.55093724531377 25.851670741646295 25.628905188807387 48 17.64737842977452 38.63080684596577 23.34691659875034 20.37489812550937 49 12.79899743911077 23.51114259249169 19.9585898763145 43.73127009208304 50 21.51017299532151 36.08965292133609 24.774235665324774 17.625938418017626 51 18.659114427318773 41.71886062850607 23.958390065900552 15.663634878274605 52 21.665940292002613 37.53541076487252 24.373501852255394 16.425147090869473 53 18.913918815975624 36.20089237131353 27.930133855697033 16.95505495701382 54 16.108331520556884 31.884924951055037 21.75331738090059 30.253426147487495 55 21.32832898172324 37.27154046997389 23.455178416013926 17.944952132288947 56 22.713671726239053 36.33099396115554 23.018334149393397 17.937000163212012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.5 13 36.0 14 67.0 15 145.0 16 223.0 17 343.5 18 464.0 19 491.0 20 518.0 21 526.0 22 507.0 23 480.0 24 456.0 25 432.0 26 427.0 27 422.0 28 390.5 29 373.5 30 388.0 31 395.5 32 403.0 33 410.0 34 417.0 35 379.0 36 341.0 37 327.5 38 277.0 39 240.0 40 234.5 41 229.0 42 262.0 43 295.0 44 300.0 45 305.0 46 308.5 47 344.5 48 377.0 49 465.0 50 553.0 51 570.5 52 588.0 53 629.5 54 713.0 55 755.0 56 761.5 57 768.0 58 816.0 59 864.0 60 913.5 61 963.0 62 1026.5 63 1050.5 64 1011.0 65 975.0 66 939.0 67 890.0 68 841.0 69 763.0 70 685.0 71 595.5 72 447.5 73 389.0 74 324.0 75 259.0 76 217.5 77 176.0 78 153.5 79 95.0 80 59.0 81 44.5 82 30.0 83 21.0 84 12.0 85 6.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.027150304083405737 15 0.20634231103388356 16 0.03258036490008688 17 0.021720243266724587 18 0.005430060816681147 19 0.0 20 0.01629018245004344 21 0.01629018245004344 22 0.005430060816681147 23 0.0 24 0.0 25 0.0 26 0.13575152041702868 27 0.021720243266724587 28 0.13575152041702868 29 0.027150304083405737 30 0.01629018245004344 31 0.17919200695047785 32 0.010860121633362294 33 0.14118158123370983 34 0.027150304083405737 35 0.010860121633362294 36 0.13032145960034752 37 0.1954821894005213 38 0.10317115551694178 39 0.34209383145091227 40 0.6190269331016508 41 0.8362293657688966 42 0.1737619461337967 43 0.3909643788010426 44 0.47784535186794097 45 0.09231103388357949 46 0.07059079061685491 47 0.05973066898349262 48 0.05973066898349262 49 0.34209383145091227 50 0.18462206776715898 51 0.29865334491746304 52 0.3258036490008688 53 0.20634231103388356 54 0.1520417028670721 55 0.1737619461337967 56 0.19005212858384013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 18416.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.87641181581233 #Duplication Level Percentage of deduplicated Percentage of total 1 92.72209927284224 79.62641181581233 2 4.723363895036358 8.112510860121633 3 1.2835915270312994 3.3069070373588185 4 0.4362946569712299 1.4986967854039965 5 0.19601644008852356 0.8416594265855778 6 0.15807777426493835 0.814509122502172 7 0.11381599747075562 0.6841876629018245 8 0.10116977552956054 0.6950477845351868 9 0.025292443882390134 0.1954821894005213 >10 0.22763199494151123 3.41007819287576 >50 0.012646221941195067 0.814509122502172 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 95 0.5158557775847089 No Hit AAGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 55 0.29865334491746304 No Hit AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 34 0.18462206776715898 No Hit AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 30 0.1629018245004344 No Hit AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 29 0.15747176368375326 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 29 0.15747176368375326 No Hit AAGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 28 0.1520417028670721 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 28 0.1520417028670721 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 28 0.1520417028670721 No Hit AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 26 0.14118158123370983 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 24 0.13032145960034752 No Hit AAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACAAAAAAA 23 0.12489139878366637 No Hit AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 19 0.10317115551694178 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.005430060816681147 0.0 0.0 0.0 0.0 11 0.01629018245004344 0.0 0.0 0.0 0.0 12 0.03258036490008688 0.0 0.0 0.0 0.0 13 0.03258036490008688 0.0 0.0 0.0 0.0 14 0.03801042571676803 0.0 0.0 0.0 0.0 15 0.03801042571676803 0.0 0.0 0.0 0.0 16 0.03801042571676803 0.0 0.0 0.0 0.0 17 0.03801042571676803 0.0 0.0 0.0 0.0 18 0.05430060816681147 0.0 0.0 0.0 0.0 19 0.059730668983492614 0.0 0.0 0.0 0.0 20 0.059730668983492614 0.0 0.0 0.0 0.0 21 0.059730668983492614 0.0 0.0 0.0 0.0 22 0.059730668983492614 0.0 0.0 0.0 0.0 23 0.059730668983492614 0.0 0.0 0.0 0.0 24 0.07059079061685491 0.0 0.0 0.0 0.0 25 0.08145091225021721 0.0 0.0 0.0 0.0 26 0.08145091225021721 0.0 0.0 0.0 0.0 27 0.08688097306689835 0.0 0.0 0.0 0.0 28 0.0923110338835795 0.0 0.0 0.0 0.0 29 0.0923110338835795 0.0 0.0 0.0 0.0 30 0.0923110338835795 0.0 0.0 0.0 0.0 31 0.0923110338835795 0.0 0.0 0.0 0.0 32 0.0923110338835795 0.0 0.0 0.0 0.0 33 0.0923110338835795 0.0 0.0 0.0 0.0 34 0.0923110338835795 0.0 0.0 0.0 0.0 35 0.0923110338835795 0.0 0.0 0.0 0.0 36 0.0923110338835795 0.0 0.0 0.0 0.0 37 0.0923110338835795 0.0 0.0 0.0 0.0 38 0.0923110338835795 0.0 0.0 0.0 0.0 39 0.0923110338835795 0.0 0.0 0.0 0.0 40 0.0923110338835795 0.0 0.0 0.0 0.0 41 0.0923110338835795 0.0 0.0 0.0 0.0 42 0.09774109470026064 0.0 0.0 0.0 0.0 43 0.1031711555169418 0.0 0.0 0.0 0.0 44 0.1031711555169418 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTCT 15 0.007823413 50.750004 39 GCACCGA 15 0.007823413 50.750004 40 ACCAACC 20 3.7856345E-4 50.75 38 ACAAACC 25 1.8368171E-5 50.749996 38 CAACCAA 25 1.8368171E-5 50.749996 40 CAAACCA 25 1.8368171E-5 50.749996 39 TATGCTT 15 0.007910034 50.60942 35 ATGCTTG 15 0.007910034 50.60942 36 CATATGC 25 1.9617935E-5 50.19231 33 CCTCGCT 15 0.008174178 50.192307 44 GGTTGAT 25 1.994114E-5 50.054794 15 CCAACAC 45 1.200533E-10 50.054794 43 TTCGTGG 20 4.053794E-4 50.054794 15 AATTCGT 15 0.008263667 50.054794 13 GTACCTG 25 1.994114E-5 50.054794 9 AATTCCT 15 0.008263667 50.054794 13 GGACCCA 15 0.008263667 50.054794 9 CTCTTTC 15 0.008263667 50.054794 11 CATTTGA 15 0.008263667 50.054794 46 ACCTGGT 25 1.994114E-5 50.054794 11 >>END_MODULE