FastQCFastQC Report
Fri 17 Jun 2016
SRR1526275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15802
Sequences flagged as poor quality0
Sequence length56
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC810.5125933426148589No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC310.19617769902543983No Hit
TTTAGGGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA290.18352107328186304No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA270.17086444753828628No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC260.1645361346664979No Hit
TTTAGGGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC220.1392228831793444No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA200.12656625743576763No Hit
TTTAGGGGGCTCGTCTTAATCAGCAACATCTTTTGATACAAACCAAAACAAAAAAA200.12656625743576763No Hit
TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGCCCTGTGCGTACCCGTC180.11390963169219087No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA180.11390963169219087No Hit
TTTAGGGGGGCCGCCTAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC170.10758131882040248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATTT150.007717945650.8899738
CCAACCT150.007717945650.8899740
TCTTTCT203.71784E-450.8899738
CAACCTG150.00781764350.72580741
GCCGGCC203.777989E-450.72580335
GGCCGGC251.9014951E-550.40064234
CAGCATT204.0263642E-450.07961743
GCAGCAT204.0263642E-450.07961742
TATTTTC204.0263642E-450.07961743
CCAAAAC650.050.07961343
TGCACTC150.00833057349.92063550
CATTTGA204.0904398E-449.92063546
TGATATG309.938121E-749.92063550
AGCATTT204.0904398E-449.92063544
GCATTTG204.0904398E-449.92063545
TCCGACT150.00833057349.92063530
CCGACTC204.0904398E-449.92063531
ATTTTCT204.0904398E-449.92063544
CGACTCA204.0904398E-449.92063532
TTAGGGA355.0438757E-849.762662
Produced by FastQC (version 0.11.5)