FastQCFastQC Report
Fri 17 Jun 2016
SRR1526274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53944
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9541.7685006673587425No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2620.48568886252409904No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2460.456028473973009No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2340.4337831825596915No Hit
CCTTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC880.1631321370309951No Hit
CCTTTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC620.11493400563547382No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC600.11122645706658757No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG570.10566513421325818No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG540.10010381135992881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCG150.00812297850.4065739
CCCTCGC150.00812297850.4065739
CCTTCCG150.00815320950.35928741
CGTCTCA251.975094E-550.35928741
CCAAAAC505.456968E-1250.26498443
TGCTCGT251.9971667E-550.26498437
CGGAGAG150.00821392350.2649843
CACGACA150.00821392350.2649844
ACGATTC204.0786454E-450.17103248
CGATTCA204.0786454E-450.17103249
ACGATTA451.2732926E-1050.1710348
CGACAGA150.00827497250.17102446
TGCATGC150.00827497250.17102447
GCAGAAC150.00827497250.17102448
CAGAACC150.00827497250.17102449
CGCAACC150.00827497250.17102449
TATGCTC252.0306587E-550.12418435
ACGACAG150.00830562350.1241845
CAGGACG150.00830562350.1241836
TGGAAGG150.00836717850.03075415