##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526274_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53944 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.924347471451874 39.0 39.0 40.0 38.0 40.0 2 38.843541450393 39.0 39.0 40.0 38.0 40.0 3 38.66493029808691 39.0 39.0 40.0 38.0 40.0 4 38.71055168322705 39.0 39.0 40.0 38.0 40.0 5 38.71314696722527 39.0 39.0 40.0 38.0 40.0 6 38.26321741064808 39.0 38.0 40.0 35.0 40.0 7 38.47477013198873 39.0 38.0 40.0 36.0 40.0 8 38.460755598398336 39.0 38.0 40.0 36.0 40.0 9 38.54070888328637 39.0 38.0 40.0 36.0 40.0 10 38.097286074447574 39.0 38.0 40.0 35.0 40.0 11 36.896577932670915 39.0 36.0 40.0 32.0 40.0 12 35.008193682337236 38.0 35.0 40.0 24.0 40.0 13 34.79321147857037 38.0 34.0 40.0 23.0 40.0 14 34.77009491324336 38.0 33.0 40.0 23.0 40.0 15 34.658942607148155 38.0 33.0 40.0 23.0 40.0 16 34.06445573187009 38.0 33.0 40.0 22.0 40.0 17 34.0462702061397 38.0 32.0 39.0 22.0 40.0 18 33.55437119976272 38.0 31.0 39.0 19.0 40.0 19 34.45185748183301 38.0 33.0 39.0 24.0 40.0 20 34.49026768500667 38.0 33.0 39.0 25.0 40.0 21 35.3781699540264 38.0 35.0 40.0 27.0 40.0 22 35.02752854812398 38.0 34.0 39.0 25.0 40.0 23 35.538095061545306 38.0 35.0 40.0 27.0 40.0 24 35.06981313955213 38.0 34.0 40.0 25.0 40.0 25 35.306113747590096 38.0 35.0 40.0 27.0 40.0 26 35.854219190271394 38.0 35.0 40.0 29.0 40.0 27 35.721210885362595 38.0 35.0 40.0 29.0 40.0 28 35.6295046715112 38.0 35.0 39.0 28.0 40.0 29 35.49293711997627 38.0 35.0 39.0 28.0 40.0 30 35.27867788818033 38.0 35.0 39.0 27.0 40.0 31 35.67712813287854 38.0 35.0 39.0 29.0 40.0 32 35.34385659202135 38.0 35.0 39.0 28.0 40.0 33 35.551127094764944 38.0 35.0 39.0 29.0 40.0 34 35.54295195017055 38.0 35.0 39.0 29.0 40.0 35 35.372682782144445 38.0 35.0 39.0 29.0 40.0 36 34.811137475900935 38.0 34.0 39.0 28.0 40.0 37 34.60598027584162 38.0 34.0 39.0 28.0 40.0 38 34.35883508823966 38.0 33.0 39.0 27.0 40.0 39 33.96702135547976 37.0 33.0 39.0 26.0 40.0 40 30.658219635177222 35.0 27.0 39.0 10.0 40.0 41 27.44151342132582 33.0 17.0 38.0 7.0 40.0 42 31.559635918730535 35.0 30.0 38.0 17.0 40.0 43 29.963387957882247 34.0 27.0 38.0 8.0 40.0 44 30.031866379949577 35.0 27.0 38.0 7.0 40.0 45 31.30898709773098 35.0 30.0 38.0 13.0 40.0 46 30.95452691680261 35.0 29.0 38.0 4.0 40.0 47 30.529530624351178 35.0 28.0 38.0 4.0 40.0 48 30.576913095061546 35.0 28.0 38.0 4.0 40.0 49 28.045769687082903 34.0 18.0 38.0 4.0 40.0 50 30.2966038855109 35.0 28.0 38.0 4.0 40.0 51 30.644816847100696 35.0 29.0 39.0 4.0 40.0 52 30.39390849770132 35.0 29.0 39.0 4.0 40.0 53 29.94579563992288 35.0 28.0 39.0 4.0 40.0 54 28.931280587275694 35.0 24.0 39.0 4.0 40.0 55 29.39607741361412 35.0 27.0 39.0 4.0 40.0 56 28.499017499629247 35.0 23.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.516274101671101 1 2 -0.42896453671148294 1 3 -0.2564191802484146 1 4 -0.26383378071883357 1 5 -0.2579352818550049 1 6 0.0283641405107673 1 7 -0.06882381512797764 1 8 -0.09531998813591969 1 9 -0.21107411021345257 1 10 0.07411905312132205 1 11 -1.944876166839883 1 12 -12.537886214716472 1 13 -8.083606369441103 1 14 -3.8269972344378473 1 15 -5.551278628613222 1 16 -23.572109363709437 1 17 -4.418788992091471 1 18 -14.926674386342 1 19 -4.388716342021446 1 20 -4.769536922484292 1 21 -5.142592969773453 1 22 -1.7089826993673185 1 23 -2.3299790265231266 1 24 -2.4718306335690237 1 25 -2.151132269699062 1 26 2.369535264279982 1 27 2.442805021690077 1 28 2.4448849077121224 1 29 2.9560219064946764 1 30 2.3062974529805373 1 31 2.50989107741902 1 32 2.512237559319459 1 33 2.513207964164984 1 34 1.6499141655645104 1 35 1.4402432007603139 1 36 2.5695647634311243 1 37 2.7896175865145594 1 38 3.1792420174993197 1 39 3.363640405366077 1 40 7.237964057509959 1 41 -9.65886824307001 1 42 -8.81349868378284 1 43 -18.884633353850994 1 44 -22.580360185462943 1 45 -12.24291211569695 1 46 -18.733498973369578 1 47 -23.95413752966953 1 48 -16.03404054696359 1 49 -21.22529510968087 1 50 -15.26554482299254 1 51 -11.78682690543323 1 52 -12.312756299126566 1 53 -11.809019764956531 1 54 -10.903525981766848 1 55 -11.581974268555737 1 56 -6.854509355032405 2 1 0.04425657826256213 2 2 0.20101058766663016 2 3 0.2954050320235595 2 4 0.2689191214370581 2 5 -0.13977607787490598 2 6 0.46969913221889925 2 7 0.6229257702783286 2 8 0.4585771926269331 2 9 0.3737103010634897 2 10 -0.01792074787370268 2 11 0.7184737502413796 2 12 -3.177189697303536 2 13 -1.7050823230066072 2 14 2.2824555018805626 2 15 1.8071376234597452 2 16 2.5638773692922214 2 17 2.816492931623287 2 18 -1.3899828440534456 2 19 2.4033649183433994 2 20 1.9450153163216797 2 21 1.842274360243131 2 22 2.3196242658068087 2 23 1.5725914544055684 2 24 1.619379980029649 2 25 1.7812889574982833 2 26 2.2627773869997156 2 27 1.8762627331328616 2 28 2.1679363173306996 2 29 1.9798610441397884 2 30 2.360401930592481 2 31 1.1232824538700967 2 32 1.459169565952962 2 33 0.6498165877139073 2 34 0.972675359594362 2 35 1.1083609453705918 2 36 0.8525249624360995 2 37 1.161093540080067 2 38 1.148976677532488 2 39 1.8016586474888001 2 40 3.7607667109096248 2 41 -8.123005888177808 2 42 -7.662171983948678 2 43 -14.666142474912355 2 44 -14.985003634882514 2 45 -6.316087903424975 2 46 -10.334037945177208 2 47 -17.987512322372684 2 48 -7.946975870346677 2 49 -12.421812522616195 2 50 -7.273422103257879 2 51 -1.6792398407566154 2 52 -1.6276402128910767 2 53 -1.013829051855371 2 54 -0.2698195141051585 2 55 -0.8000505537962006 2 56 3.077289981617678 3 1 0.22203435604034638 3 2 0.2826772543332936 3 3 0.31651614313467036 3 4 0.34114134365927384 3 5 0.29689058879176145 3 6 0.5302546877744518 3 7 0.5057035480561041 3 8 0.4941327481824871 3 9 0.5031547455079348 3 10 0.09541258545962705 3 11 1.9540293057969293 3 12 3.6694769693631315 3 13 1.8338065658822842 3 14 3.1091221685472235 3 15 3.7165820679041914 3 16 4.159432924847778 3 17 4.542604042734396 3 18 4.8950171559465545 3 19 4.0172538072322865 3 20 3.9733486496550157 3 21 3.799496582465352 3 22 3.7029575991401416 3 23 3.707035898850009 3 24 3.8549355355852057 3 25 2.675733401942729 3 26 2.738888498110825 3 27 3.0179293997995273 3 28 2.3368252062195864 3 29 3.2459721552508967 3 30 3.0854019305924822 3 31 2.19606023164787 3 32 1.8280584548418446 3 33 1.8803721432694616 3 34 1.6176753595943651 3 35 1.314472056481705 3 36 3.137524962436096 3 37 3.232204651191182 3 38 3.863421121976934 3 39 3.151103091933244 3 40 6.872988933131847 3 41 7.5775496673777525 3 42 4.378939127162436 3 43 -1.471698030467909 3 44 -0.11389252377140124 3 45 4.663356541019464 3 46 2.8854064992672406 3 47 -1.688067877928237 3 48 1.899690796319991 3 49 3.838187477383805 3 50 1.6210223411865634 3 51 3.4290934925767225 3 52 3.9262486759978152 3 53 3.8550598370335187 3 54 4.368513819228173 3 55 4.3577272239815805 3 56 4.816734426062119 4 1 0.5303676893736764 4 2 0.5760105876666302 4 3 0.5731828098013381 4 4 0.6161413436592795 4 5 0.6518905887917654 4 6 0.9280324655522278 4 7 0.6645924369449929 4 8 0.8196883037380402 4 9 0.3737103010634897 4 10 0.8431903632374045 4 11 2.6073626391302653 4 12 4.2533658582520175 4 13 4.364362121437836 4 14 4.532455501880563 4 15 4.696026512348638 4 16 5.494432924847779 4 17 5.26093737606773 4 18 5.846128267057665 4 19 4.917253807232285 4 20 4.486681982988344 4 21 4.258941026909795 4 22 4.375179821362366 4 23 3.3781470099611255 4 24 4.327713313362985 4 25 4.170177846387169 4 26 3.1377773869997156 4 27 3.27904051091064 4 28 2.9734918728862496 4 29 3.2854165996953455 4 30 3.9437352639258165 4 31 3.109393564981204 4 32 2.8619473437307335 4 33 2.2609276988250215 4 34 2.1115642484832478 4 35 2.525027612037256 4 36 2.644191629102764 4 37 2.7444268734134027 4 38 3.7461988997547095 4 39 3.746103091933243 4 40 6.857988933131846 4 41 10.15477188959997 4 42 5.726716904940215 4 43 7.069968636198759 4 44 7.626107476228601 4 45 5.961689874352803 4 46 6.332628721489456 4 47 5.234709899849538 4 48 5.941913018542213 4 49 8.39763192182825 4 50 6.30991123007545 4 51 6.04298238146561 4 52 6.2334708982200375 4 53 5.416726503700186 4 54 7.119069374783731 4 55 6.644393890648242 4 56 5.868956648284346 5 1 0.737457241612482 5 2 0.809841433437775 5 3 0.9226852973635218 5 4 0.9898975625647495 5 5 0.9808706882942531 5 6 1.476125334540619 5 7 0.88640006546904 5 8 1.0987098626103489 5 9 0.8784781285925121 5 10 1.4024772620765447 5 11 2.7566163704735516 5 12 4.671069009164121 5 13 4.737289152946957 5 14 4.814793810338273 5 15 5.2994676400434955 5 16 5.935353322857729 5 17 5.9394199631324085 5 18 5.6852659121654625 5 19 4.3426269415606455 5 20 5.021507853635114 5 21 3.857407030226547 5 22 4.231315808095367 5 23 3.6550956003425483 5 24 4.155035038072768 5 25 3.6399125064203375 5 26 3.130729294130724 5 27 3.293551290346791 5 28 3.3404072957718256 5 29 3.015723399031991 5 30 3.3062974529805373 5 31 3.390488092344391 5 32 4.109252484692597 5 33 2.916193038791853 5 34 3.0678246133257048 5 35 2.9178551410588156 5 36 3.285982673878884 5 37 4.222453407410086 5 38 3.8061076891411076 5 39 4.0651329426795115 5 40 7.670799878405479 5 41 9.953072055437453 5 42 6.12679982367985 5 43 7.264620377492285 5 44 7.688296530954968 5 45 5.90634161564633 5 46 5.848590578869228 5 47 5.7473550076439 5 48 5.354019154528945 5 49 8.356794442557934 5 50 5.824007415813426 5 51 4.019143243820498 5 52 4.732019820276424 5 53 5.041726503700186 5 54 5.768115809277926 5 55 4.716533194130829 5 56 4.966386167355651 6 1 0.5928676893736764 6 2 0.43017725433329446 6 3 0.6304744764680024 6 4 0.6421830103259438 6 5 0.8810572554584297 6 6 1.217963021107785 6 7 0.6923702147227715 6 8 0.9776744148491545 6 9 1.000446412174604 6 10 0.7737459187929616 6 11 2.1386126391302653 6 12 4.421768636029796 6 13 4.501514899215614 6 14 4.777247168547227 6 15 4.959915401237524 6 16 5.176724591514443 6 17 5.375520709401066 6 18 6.167308822613222 6 19 4.854753807232285 6 20 4.38251531632168 6 21 3.8613715824653525 6 22 4.309207599140144 6 23 3.470160898850011 6 24 4.16972720225187 6 25 3.725733401942726 6 26 3.0353468314441585 6 27 3.2095960664661973 6 28 2.9943252062195853 6 29 3.7368054885842312 6 30 3.391651930592481 6 31 2.5677268983145396 6 32 3.3011834548418477 6 33 2.8459971432694644 6 34 3.332050359594362 6 35 3.4173887231483704 6 36 3.409816629102764 6 37 3.5013713178578456 6 38 3.827796121976931 6 39 4.417978091933243 6 40 8.285072266465182 6 41 5.073174667377749 6 42 6.115605793829101 6 43 7.236635302865423 6 44 7.339649142895265 6 45 5.675231541019468 6 46 6.349989832600571 6 47 6.165265455405095 6 48 6.125940796319991 6 49 8.744854144050471 6 50 5.780397341186564 6 51 6.4093018259100525 6 52 6.56854034266448 6 53 5.947976503700186 6 54 7.256222152561509 6 55 6.618352223981578 6 56 7.266526092728789 7 1 0.6397426893736764 7 2 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19 4.750587140565621 9 20 4.607894104200469 9 21 4.136466279435048 9 22 4.115078811261355 9 23 3.524611656425769 9 24 4.008268868918535 9 25 3.415127341336664 9 26 2.847373346595674 9 27 3.0845960664661973 9 28 3.1174312668256476 9 29 3.2841539734327156 9 30 3.3793413245318717 9 31 2.745757201344844 9 32 2.938967545750941 9 33 2.3227963856937066 9 34 2.17343293535194 9 35 3.064169026178675 9 36 2.7199492048603418 9 37 3.181295560282088 9 38 3.1919059704617823 9 39 2.901406122236274 9 40 5.899655599798518 9 41 2.224216334044417 9 42 4.8353027635260695 9 43 6.285877727107845 9 44 6.004895355016476 9 45 4.8669929046558345 9 46 4.546012559873297 9 47 4.99859878873843 9 48 5.6742362508654445 9 49 8.017581416777745 9 50 4.951325371489595 9 51 4.968487431970662 9 52 4.719582009331145 9 53 5.117484079457764 9 54 5.779422910137267 9 55 4.583787830042187 9 56 3.942188971516668 10 1 0.5887010227070064 10 2 0.7426772543332945 10 3 0.9898494764680024 10 4 0.8578080103259467 10 5 0.9435572554584297 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120 16 -20.331547467309086 120 17 -16.118964584716583 120 18 -17.510080883269133 120 19 -8.229805016297128 120 20 -14.415278801325378 120 21 -5.2957975351817055 120 22 -3.391160047918678 120 23 -4.174140571738224 120 24 -10.599574268336365 120 25 -3.906619539233745 120 26 1.2733983020323905 120 27 1.2610666547014944 120 28 0.9980016768078173 120 29 1.0051878415254052 120 30 1.5736372247101258 120 31 -0.8219789840384024 120 32 -11.210765074569917 120 33 -0.06668668025994862 120 34 -0.931736405111522 120 35 -1.5035671592045716 120 36 -0.5911024885442941 120 37 0.08593014138725863 120 38 -0.42128476037601104 120 39 0.371103091933243 120 40 2.654557560582827 120 41 -0.7071562149751927 120 42 -4.840276559112077 120 43 -11.697188226546341 120 44 -9.795461151222383 120 45 -9.173114047215826 120 46 -16.44596604975237 120 47 -16.481793368124315 120 48 -13.69207390956236 120 49 -18.372792914773058 120 50 -11.990742364695787 120 51 -11.90227905644289 120 52 -11.623555245570813 120 53 -12.487685261005698 120 54 -13.507564612144373 120 55 -11.963449246606658 120 56 -8.318951848447682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 36.0 5 7.0 6 16.0 7 12.0 8 58.0 9 185.0 10 149.0 11 104.0 12 80.0 13 59.0 14 60.0 15 76.0 16 92.0 17 120.0 18 200.0 19 226.0 20 300.0 21 389.0 22 488.0 23 568.0 24 724.0 25 900.0 26 947.0 27 1043.0 28 1273.0 29 1485.0 30 1897.0 31 2304.0 32 2569.0 33 2933.0 34 3581.0 35 4307.0 36 5422.0 37 7200.0 38 8013.0 39 6110.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.35303277472934 0.367047308319739 0.22986801127094764 0.050051905679964405 8 99.33449503188491 0.4337831825596915 0.14274061990212072 0.08898116565327006 9 98.2203766869346 0.7600474566216817 0.6358445795639923 0.38373127687972713 10 39.754189529882844 38.480646596470415 12.151490434524693 9.613673439122053 11 27.741732166691385 35.266201987246035 21.5742251223491 15.41784072371348 12 31.57348361263533 14.450170547234167 23.14993326412576 30.826412576004746 13 21.81457518399733 15.322296583303979 27.25941306242005 35.60371517027864 14 20.000741523459947 21.16864097287878 33.63550414326234 25.195113360398942 15 27.942761568481693 20.78981601932726 26.305519420182122 24.96190299200892 16 29.883692889869966 23.098183976701478 25.69886289858836 21.319260234840193 17 24.558801720302537 22.844060507192644 29.254411982796974 23.342725789707846 18 20.52204219346706 23.990582477475808 29.982203107040895 25.50517222201624 19 27.79510022271715 25.885300668151444 23.648849294729025 22.670749814402374 20 24.226450116009282 29.549883990719255 23.584222737819026 22.639443155452437 21 24.334495031884916 22.89596618715705 29.272949725641407 23.496589055316626 22 23.668990063769833 22.83479163577043 22.96084828711256 30.535370013347173 23 20.634361560136437 26.46633545899451 24.88691976864897 28.012383212220083 24 20.460106777398785 26.266127836274656 31.12857778436898 22.145187601957588 25 27.11986948945163 27.55923028437952 24.198212895332023 21.122687330836825 26 20.886944269346287 26.256071786124814 27.006933887055506 25.850050057473396 27 22.0197108335344 23.549063642606583 27.019803634068936 27.411421889790084 28 21.139780954148875 28.22350102097643 28.943753480601448 21.69296454427325 29 26.653808349251683 23.98879842732887 27.877821257024166 21.479571966395284 30 20.286281126582985 24.802996310236775 35.423581109895615 19.487141453284632 31 19.300880853036624 30.840982846546126 31.528975428836347 18.3291608715809 32 24.753228439958438 23.99250408193558 32.24914650437881 19.005120973727177 33 18.56171761821626 24.445600089149735 34.0886296942907 22.9040525983433 34 17.102895620397337 29.789089020386207 35.85672151217793 17.25129384703853 35 17.510341117768174 24.840941551816883 41.920943777707706 15.727773552707239 36 18.326471570045623 29.72812581135715 35.64778754497237 16.297615073624865 37 17.189735585409814 24.71895497705186 41.40513220729509 16.68617723024323 38 23.059471119872057 25.748502994011975 35.266113735262394 15.925912150853572 39 18.428677563150075 25.562778603268942 34.463224368499255 21.545319465081725 40 14.001045829753856 19.799798304261756 34.73462069995891 31.464535166025478 41 12.856635048923312 18.173654375035078 33.054573347551965 35.91513722848965 42 21.46955601359256 25.655023861251923 35.424210350398305 17.45120977475721 43 14.770974635324723 21.499581900956983 40.02415683359658 23.705286630121712 44 15.365267452909285 23.01902259981741 33.45630018817655 28.159409759096754 45 16.538740312598733 26.485401527682274 40.39437247941718 16.581485680301817 46 16.27375578219919 27.035612773783647 35.87285663861488 20.81777480540229 47 15.80109062581148 30.005193456245134 36.20951886337501 17.984197054568387 48 16.408237544335297 31.548160665540102 35.141408701788265 16.902193088336332 49 13.896498388028549 25.053577085779246 30.89208177260953 30.157842753582674 50 20.264071756207173 27.062712399487452 36.58378057159835 16.089435272707014 51 16.79936009524164 31.818520034227465 35.61888463112467 15.763235239406228 52 16.744021981286203 27.044036833506606 39.15973563047675 17.05220555473043 53 16.68245917172956 27.413930847407244 38.55043382940379 17.35317615145941 54 16.210792329418762 29.36673108369258 32.860115950646644 21.56236063624201 55 16.470828840218644 32.38946937864872 35.386531811252 15.753169969880638 56 21.101224201668185 27.315115825453734 34.53214690420018 17.0515130686779 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 5.5 7 3.0 8 3.5 9 4.0 10 15.5 11 27.0 12 96.0 13 260.0 14 355.0 15 425.5 16 496.0 17 535.5 18 575.0 19 592.0 20 609.0 21 624.5 22 775.5 23 911.0 24 1067.5 25 1224.0 26 1370.0 27 1516.0 28 1747.0 29 2082.5 30 2187.0 31 2288.0 32 2389.0 33 2235.5 34 2082.0 35 1792.0 36 1502.0 37 1412.5 38 1300.5 39 1278.0 40 1194.5 41 1111.0 42 1245.5 43 1380.0 44 1554.5 45 1729.0 46 2338.0 47 2890.5 48 2834.0 49 2443.0 50 2052.0 51 2051.5 52 2051.0 53 2142.0 54 2273.5 55 2314.0 56 2217.5 57 2121.0 58 2175.0 59 2229.0 60 2299.0 61 2369.0 62 2240.5 63 1969.0 64 1826.0 65 1666.0 66 1506.0 67 1323.5 68 1141.0 69 979.0 70 817.0 71 737.5 72 596.5 73 535.0 74 416.0 75 297.0 76 249.5 77 202.0 78 157.0 79 86.0 80 60.0 81 42.0 82 24.0 83 16.5 84 9.0 85 5.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.005561322853329379 14 0.001853774284443126 15 0.24840575411537888 16 0.06488209995550942 17 0.0 18 0.003707548568886252 19 0.11864155420436007 20 0.12791042562657573 21 0.0 22 0.0 23 0.0 24 0.0 25 0.003707548568886252 26 0.011122645706658758 27 0.12049532848880319 28 0.13717929704879134 29 0.04263680854219191 30 0.020391517128874388 31 0.035221711404419404 32 0.08898116565327006 33 0.18908497701319887 34 0.06488209995550942 35 0.06117455138662316 36 0.040783034257748776 37 0.235429334124277 38 0.3151416283553315 39 0.19279252558208512 40 0.7378021652083643 41 0.9139107222304612 42 0.1686934598843245 43 0.23913688269316327 44 0.5023728310840871 45 0.2539670769687083 46 0.2131840427109595 47 0.055613228533293785 48 0.17240100845321074 49 0.5246181224974047 50 0.176108557022097 51 0.3448020169064215 52 0.1483019427554501 53 0.22430668841761825 54 0.23728310840872013 55 0.29289633694201395 56 0.20947649414207326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 53944.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.51408868456177 #Duplication Level Percentage of deduplicated Percentage of total 1 85.39509676099905 61.923476197538186 2 8.714880998031546 12.639033071333234 3 2.8376409233836952 6.173068367195611 4 1.086484137331595 3.151416283553315 5 0.6263261497558607 2.2708734984428296 6 0.33233632436025257 1.4459439418656386 7 0.2326354270521768 1.1808542191902713 8 0.1942889280875323 1.127094764941421 9 0.0945880307794565 0.617306836719561 >10 0.46015798757573434 5.63362005042266 >50 0.01533859958585781 0.6933115823817292 >100 0.007669299792928905 1.3755005190567997 >500 0.0025564332643096353 1.7685006673587425 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 954 1.7685006673587425 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 262 0.48568886252409904 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 246 0.456028473973009 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 234 0.4337831825596915 No Hit CCTTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 88 0.1631321370309951 No Hit CCTTTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 62 0.11493400563547382 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 60 0.11122645706658757 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 57 0.10566513421325818 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 54 0.10010381135992881 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0018537742844431263 0.0 0.0 0.0 0.0 10 0.007415097137772505 0.0 0.0 0.0 0.0 11 0.011122645706658758 0.0 0.0 0.0 0.0 12 0.011122645706658758 0.0 0.0 0.0 0.0 13 0.011122645706658758 0.0 0.0 0.0 0.0 14 0.016683968559988136 0.0 0.0 0.0 0.0 15 0.022245291413317516 0.0 0.0 0.0 0.0 16 0.02409906569776064 0.0 0.0 0.0 0.0 17 0.025952839982203768 0.0 0.0 0.0 0.0 18 0.02966038855109002 0.0 0.0 0.0 0.0 19 0.03892925997330565 0.0 0.0 0.0 0.0 20 0.0426368085421919 0.0 0.0 0.0 0.0 21 0.046344357111078156 0.0 0.0 0.0 0.0 22 0.05375945424885066 0.0 0.0 0.0 0.0 23 0.061174551386623165 0.0 0.0 0.0 0.0 24 0.07600474566216818 0.0 0.0 0.0 0.0 25 0.08712739136882693 0.0 0.0 0.0 0.0 26 0.09083493993771319 0.0 0.0 0.0 0.0 27 0.09639626279104256 0.0 0.0 0.0 0.0 28 0.10010381135992881 0.0 0.0 0.0 0.0 29 0.10751890849770132 0.0 0.0 0.0 0.0 30 0.12420287705768945 0.0 0.0 0.0 0.0 31 0.1279104256265757 0.0 0.0 0.0 0.0 32 0.1279104256265757 0.0 0.0 0.0 0.0 33 0.12976419991101884 0.0 0.0 0.0 0.0 34 0.13161797419546195 0.0 0.0 0.0 0.0 35 0.1353255227643482 0.0 0.0 0.0 0.0 36 0.13717929704879134 0.0 0.0 0.0 0.0 37 0.13903307133323448 0.0 0.0 0.0 0.0 38 0.14274061990212072 0.0 0.0 0.0 0.0 39 0.14459439418656383 0.0 0.0 0.0 0.0 40 0.14459439418656383 0.0 0.0 0.0 0.0 41 0.14644816847100697 0.0 0.0 0.0 0.0 42 0.15200949132433636 0.0 0.0 0.0 0.0 43 0.15757081417766572 0.0 0.0 0.0 0.0 44 0.16127836274655197 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTCCG 15 0.008122978 50.40657 39 CCCTCGC 15 0.008122978 50.40657 39 CCTTCCG 15 0.008153209 50.359287 41 CGTCTCA 25 1.975094E-5 50.359287 41 CCAAAAC 50 5.456968E-12 50.264984 43 TGCTCGT 25 1.9971667E-5 50.264984 37 CGGAGAG 15 0.008213923 50.26498 43 CACGACA 15 0.008213923 50.26498 44 ACGATTC 20 4.0786454E-4 50.171032 48 CGATTCA 20 4.0786454E-4 50.171032 49 ACGATTA 45 1.2732926E-10 50.17103 48 CGACAGA 15 0.008274972 50.171024 46 TGCATGC 15 0.008274972 50.171024 47 GCAGAAC 15 0.008274972 50.171024 48 CAGAACC 15 0.008274972 50.171024 49 CGCAACC 15 0.008274972 50.171024 49 TATGCTC 25 2.0306587E-5 50.124184 35 ACGACAG 15 0.008305623 50.12418 45 CAGGACG 15 0.008305623 50.12418 36 TGGAAGG 15 0.008367178 50.030754 15 >>END_MODULE